Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00272
(754 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g38220.2 68417.m05395 aminoacylase, putative / N-acyl-L-amino... 101 4e-22
At4g38220.1 68417.m05394 aminoacylase, putative / N-acyl-L-amino... 101 4e-22
At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino... 101 4e-22
At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino... 98 5e-21
At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si... 44 1e-04
At1g56570.1 68414.m06506 pentatricopeptide (PPR) repeat-containi... 31 0.62
At2g27800.1 68415.m03370 pentatricopeptide (PPR) repeat-containi... 31 1.1
At4g36550.1 68417.m05190 U-box domain-containing protein low sim... 30 1.9
At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containi... 29 4.4
At3g62160.1 68416.m06984 transferase family protein low similari... 28 7.7
At1g79960.1 68414.m09346 ovate protein-related contains TIGRFAM ... 28 7.7
At1g16420.1 68414.m01964 latex-abundant protein, putative (AMC8)... 28 7.7
>At4g38220.2 68417.m05395 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 433
Score = 101 bits (243), Expect = 4e-22
Identities = 48/83 (57%), Positives = 62/83 (74%), Gaps = 1/83 (1%)
Frame = +2
Query: 257 LPSILLNSHMDVVPVFENSWKHPPFAAEIEDNV-IYARGVQDMKSVGIQYIEAVRRLKET 433
LP+ LLNSH DVVP ++ W H P A ++ + IYARG QDMK VG+QY+EA+R+L+ +
Sbjct: 84 LPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQAS 143
Query: 434 GVKLKRTVHLSFVPDEEIGGDTG 502
G K R+V+LSFVPDEEIGG G
Sbjct: 144 GFKPLRSVYLSFVPDEEIGGHDG 166
Score = 61.3 bits (142), Expect = 7e-10
Identities = 26/82 (31%), Positives = 50/82 (60%)
Frame = +3
Query: 24 LISAMLRIHTTSSVKIDPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVE 203
L+ ++ +H ++ D VS Q YLRI +V PN +Y + ++F+ ++A+ + L+ Q +E
Sbjct: 6 LLLVVVVLHLSAVAGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIE 65
Query: 204 PLPKKPTLVMTWLGEQPSCRAY 269
+ KP L++ W+G P+ A+
Sbjct: 66 FVKGKPLLLLKWVGSDPTLPAF 87
Score = 59.3 bits (137), Expect = 3e-09
Identities = 27/74 (36%), Positives = 41/74 (55%)
Frame = +1
Query: 508 EFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPDNCG 687
+F ++ +FK++N+ LDEG+ SP + Y VF GER W + I G GHG+ L ++
Sbjct: 169 KFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAM 228
Query: 688 EKLRYIIDKFMDLR 729
E L I+ R
Sbjct: 229 ENLLKSIESIRRFR 242
>At4g38220.1 68417.m05394 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 430
Score = 101 bits (243), Expect = 4e-22
Identities = 48/83 (57%), Positives = 62/83 (74%), Gaps = 1/83 (1%)
Frame = +2
Query: 257 LPSILLNSHMDVVPVFENSWKHPPFAAEIEDNV-IYARGVQDMKSVGIQYIEAVRRLKET 433
LP+ LLNSH DVVP ++ W H P A ++ + IYARG QDMK VG+QY+EA+R+L+ +
Sbjct: 84 LPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQAS 143
Query: 434 GVKLKRTVHLSFVPDEEIGGDTG 502
G K R+V+LSFVPDEEIGG G
Sbjct: 144 GFKPLRSVYLSFVPDEEIGGHDG 166
Score = 61.3 bits (142), Expect = 7e-10
Identities = 26/82 (31%), Positives = 50/82 (60%)
Frame = +3
Query: 24 LISAMLRIHTTSSVKIDPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVE 203
L+ ++ +H ++ D VS Q YLRI +V PN +Y + ++F+ ++A+ + L+ Q +E
Sbjct: 6 LLLVVVVLHLSAVAGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIE 65
Query: 204 PLPKKPTLVMTWLGEQPSCRAY 269
+ KP L++ W+G P+ A+
Sbjct: 66 FVKGKPLLLLKWVGSDPTLPAF 87
Score = 59.3 bits (137), Expect = 3e-09
Identities = 27/74 (36%), Positives = 41/74 (55%)
Frame = +1
Query: 508 EFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPDNCG 687
+F ++ +FK++N+ LDEG+ SP + Y VF GER W + I G GHG+ L ++
Sbjct: 169 KFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAM 228
Query: 688 EKLRYIIDKFMDLR 729
E L I+ R
Sbjct: 229 ENLLKSIESIRRFR 242
>At1g44820.1 68414.m05134 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 438
Score = 101 bits (243), Expect = 4e-22
Identities = 46/84 (54%), Positives = 59/84 (70%), Gaps = 1/84 (1%)
Frame = +2
Query: 257 LPSILLNSHMDVVPVFENSWKHPPFAA-EIEDNVIYARGVQDMKSVGIQYIEAVRRLKET 433
LPSIL NSH+D VP W +PPF+A + D IYARG QD K +G+QY+E++R LK
Sbjct: 89 LPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLESIRNLKSR 148
Query: 434 GVKLKRTVHLSFVPDEEIGGDTGM 505
G RT+H+S+VP+EEIGG GM
Sbjct: 149 GFSPLRTIHISYVPEEEIGGFDGM 172
Score = 64.9 bits (151), Expect = 5e-11
Identities = 28/68 (41%), Positives = 43/68 (63%)
Frame = +3
Query: 54 TSSVKIDPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVM 233
+SS + D ++ Q YLR + HPN +Y I+FL N+A+ IGL + +E + KP L++
Sbjct: 21 SSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKTIEFISGKPILLI 80
Query: 234 TWLGEQPS 257
TWLG P+
Sbjct: 81 TWLGSNPN 88
Score = 62.9 bits (146), Expect = 2e-10
Identities = 27/75 (36%), Positives = 44/75 (58%)
Frame = +1
Query: 508 EFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPDNCG 687
+F + FK++N+GFA+DEG A+P D++ VF +R+ WH I G GHG+ L ++
Sbjct: 174 KFAASSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAM 233
Query: 688 EKLRYIIDKFMDLRQ 732
E L ++ R+
Sbjct: 234 ENLMKSVELISRFRE 248
>At1g44180.1 68414.m05103 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1) [Homo sapiens] SWISS-PROT:Q03154
Length = 435
Score = 98.3 bits (234), Expect = 5e-21
Identities = 46/84 (54%), Positives = 56/84 (66%), Gaps = 1/84 (1%)
Frame = +2
Query: 257 LPSILLNSHMDVVPVFENSWKHPPFAAEIE-DNVIYARGVQDMKSVGIQYIEAVRRLKET 433
L SIL NSH+D VP W HPPF+A D IYARG QD K +G+QY+EA+R LK
Sbjct: 91 LSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIRNLKSR 150
Query: 434 GVKLKRTVHLSFVPDEEIGGDTGM 505
RT+H+S+VP+EEIGG GM
Sbjct: 151 SFSPLRTIHISYVPEEEIGGFGGM 174
Score = 63.7 bits (148), Expect = 1e-10
Identities = 28/75 (37%), Positives = 43/75 (57%)
Frame = +1
Query: 508 EFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPDNCG 687
+F + FK++N+GF +DEG ASP D++ VF ER WH+ I G GHG+ L ++
Sbjct: 176 KFAASSEFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAM 235
Query: 688 EKLRYIIDKFMDLRQ 732
E L ++ R+
Sbjct: 236 ENLMKSVELISRFRE 250
Score = 60.1 bits (139), Expect = 2e-09
Identities = 25/58 (43%), Positives = 37/58 (63%)
Frame = +3
Query: 72 DPSVSTLQNYLRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVMTWLG 245
D ++ Q YLR + HPN +Y I+FL ++A IGL + +E +P KP L++TWLG
Sbjct: 29 DTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTSRTIEFVPGKPVLLLTWLG 86
>At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein
similar to acetylornithine deacetylase
(Acetylornithinase, AO; N-acetylornithinase, NAO)
[Dictyostelium discoideum] SWISS-PROT:P54638
Length = 440
Score = 43.6 bits (98), Expect = 1e-04
Identities = 28/97 (28%), Positives = 44/97 (45%), Gaps = 3/97 (3%)
Frame = +2
Query: 227 GNDLVRRAA*LPSILLN---SHMDVVPVFENSWKHPPFAAEIEDNVIYARGVQDMKSVGI 397
GN +V +P +L+ HMDVV + W+ PF+ I+ + + RG D
Sbjct: 80 GNLIVEYPGSVPGKILSFVGMHMDVVTANPDDWEFDPFSLSIDGDKLRGRGTTDCLGHVA 139
Query: 398 QYIEAVRRLKETGVKLKRTVHLSFVPDEEIGGDTGMG 508
E +++L + LK TV F+ EE G+G
Sbjct: 140 LVTELMKKLGQAKPALKSTVVAVFIASEENSSIPGVG 176
>At1g56570.1 68414.m06506 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 611
Score = 31.5 bits (68), Expect = 0.62
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +3
Query: 117 VHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVMTWLGEQPSCRAY 269
++P+ D C+ L A KIG ++VE +P KP TW +C+A+
Sbjct: 447 INPDRDIYNCVVDLLGRAGKIGEAYELVERMPFKPD-ESTWGAILGACKAH 496
>At2g27800.1 68415.m03370 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 427
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -1
Query: 382 HILNSSSVNYIVFYFSSKGRMLPAVLEDRHHV 287
HI N + N I+FYF+ G+++ AV RH V
Sbjct: 188 HIGNENLYNSIIFYFTKAGKLIRAVNIFRHMV 219
>At4g36550.1 68417.m05190 U-box domain-containing protein low
similarity to immediate-early fungal elicitor protein
CMPG1 [Petroselinum crispum] GI:14582200; contains Pfam
profile PF04564: U-box domain
Length = 577
Score = 29.9 bits (64), Expect = 1.9
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = -1
Query: 187 CSPIFSASFLRKLMHSL*STFGCTERI--LR*FCKVETLGSILTELVVCILSIAEIRQNG 14
C + S F++KL L C I L+ C E +TE C+ SIAE+ ++
Sbjct: 396 CLEMVSLDFIQKLTSFLQQKVFCKHSIIILKNLCSTEKGRGCITETPDCLASIAELLESN 455
Query: 13 V 11
V
Sbjct: 456 V 456
>At3g14330.1 68416.m01812 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 710
Score = 28.7 bits (61), Expect = 4.4
Identities = 16/51 (31%), Positives = 26/51 (50%)
Frame = +3
Query: 117 VHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVMTWLGEQPSCRAY 269
V P +++ C+ + A KI V+V+E +P KP+ W SCR +
Sbjct: 470 VSPALEHYACLVDILGRAGKIKEAVKVIETMPFKPS-ASIWGSLLNSCRLH 519
>At3g62160.1 68416.m06984 transferase family protein low similarity
to Taxus cuspidata transferases: 10-deacetylbaccatin
III-10-O-acetyl transferase GI:6746554, taxadienol
acetyl transferase GI:6978038,
2-debenzoyl-7,13-diacetylbaccatin III-2-O-benzoyl
transferase GI:11559716; contains Pfam profile PF02458
transferase family
Length = 428
Score = 27.9 bits (59), Expect = 7.7
Identities = 10/22 (45%), Positives = 14/22 (63%)
Frame = +3
Query: 204 PLPKKPTLVMTWLGEQPSCRAY 269
PLPKK +MTW E+ C+ +
Sbjct: 393 PLPKKGVRLMTWCVEETHCQLF 414
>At1g79960.1 68414.m09346 ovate protein-related contains TIGRFAM
TIGR01568 : uncharacterized plant-specific domain
TIGR01568
Length = 294
Score = 27.9 bits (59), Expect = 7.7
Identities = 13/39 (33%), Positives = 20/39 (51%)
Frame = +1
Query: 634 TCPGKSGHGSLLLPDNCGEKLRYIIDKFMDLRQESVKKL 750
T + +L+LP+NC LRY + D RQ V+ +
Sbjct: 176 TSSERQSRSTLVLPENCIAVLRYTDEPQEDFRQSMVEMM 214
>At1g16420.1 68414.m01964 latex-abundant protein, putative (AMC8) /
caspase family protein similar to latex-abundant protein
[Hevea brasiliensis] gb:AAD13216; contains Pfam profile
PF00656: ICE-like protease (caspase) p20 domain
Length = 381
Score = 27.9 bits (59), Expect = 7.7
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = -1
Query: 445 ELDTGFLQPPHGLNVLNSNRLHILNSSSVNYIVFYFSSKGRMLPAVLED 299
+ D +QP G N+ + I + S +++VF++S G +P +ED
Sbjct: 49 DTDKSCIQPT-GKNICDELDNLIASGQSGDFLVFHYSGHGTRIPPGIED 96
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,718,565
Number of Sequences: 28952
Number of extensions: 358767
Number of successful extensions: 1006
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 961
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1002
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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