Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00271
(758 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella ve... 142 6e-33
UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Ma... 139 6e-32
UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52; cell... 136 6e-31
UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63; Euka... 133 5e-30
UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondr... 132 7e-30
UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplas... 131 2e-29
UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole geno... 130 4e-29
UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondri... 126 6e-28
UniRef50_A0L5P5 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 124 2e-27
UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|R... 123 4e-27
UniRef50_Q0AIF8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 120 3e-26
UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic... 116 5e-25
UniRef50_A7CWP9 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 116 6e-25
UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila melanogas... 116 6e-25
UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:... 112 8e-24
UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2; Ostr... 110 3e-23
UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor circinelloides... 110 3e-23
UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon nigrovirid... 110 4e-23
UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;... 109 6e-23
UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2; Ostre... 109 7e-23
UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep: ... 109 1e-22
UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep... 108 2e-22
UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:... 107 2e-22
UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;... 107 2e-22
UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Mali... 106 5e-22
UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187; cell... 105 9e-22
UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Ma... 105 1e-21
UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus californi... 104 3e-21
UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isofo... 102 8e-21
UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 102 1e-20
UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 100 3e-20
UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 100 3e-20
UniRef50_A7IMB8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 100 4e-20
UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20; Trypanoso... 99 1e-19
UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,... 97 3e-19
UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:... 97 4e-19
UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2; Mucoromyco... 96 7e-19
UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase (... 95 2e-18
UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2; Aeromo... 94 4e-18
UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 94 4e-18
UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme; ... 90 5e-17
UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n... 89 8e-17
UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2; ... 88 2e-16
UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon nigrovirid... 88 3e-16
UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:... 87 3e-16
UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6; Deinococci|... 87 6e-16
UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep: ... 85 1e-15
UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 85 2e-15
UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Mali... 85 2e-15
UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia intestinalis... 83 5e-15
UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10; Franc... 82 1e-14
UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium globosum|... 79 2e-13
UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep: ... 78 2e-13
UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|R... 77 4e-13
UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 77 5e-13
UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondri... 73 6e-12
UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococc... 72 2e-11
UniRef50_Q5C370 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 71 2e-11
UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 71 4e-11
UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3; Schizo... 65 2e-09
UniRef50_Q27113 Cluster: Malic enzyme; n=1; Trichomonas vaginali... 61 3e-08
UniRef50_A7R628 Cluster: Chromosome undetermined scaffold_1141, ... 52 1e-05
UniRef50_A5B3Q7 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 52 1e-05
UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n... 52 2e-05
UniRef50_Q3W7J3 Cluster: Malate dehydrogenase; n=1; Frankia sp. ... 43 0.007
UniRef50_A4EC16 Cluster: Putative uncharacterized protein; n=1; ... 42 0.017
UniRef50_Q8U225 Cluster: Malate oxidoreductase; n=41; cellular o... 42 0.022
UniRef50_Q5Y0M3 Cluster: Malate oxidoreductase; n=8; cellular or... 40 0.067
UniRef50_P54572 Cluster: Probable NAD-dependent malic enzyme 1; ... 40 0.067
UniRef50_Q2KKD0 Cluster: Oxalacetate decarboxylase; n=11; Lactob... 39 0.12
UniRef50_A0DE35 Cluster: Malic enzyme; n=3; Paramecium tetraurel... 39 0.12
UniRef50_Q8VW43 Cluster: Proline dehydrogenase; n=11; Proteobact... 37 0.47
UniRef50_Q9HKY7 Cluster: Malate oxidoreductase (Malic enzyme) re... 37 0.62
UniRef50_Q73UK5 Cluster: Putative uncharacterized protein; n=1; ... 36 0.82
UniRef50_A1B0A4 Cluster: Beta-ketoacyl synthase; n=1; Paracoccus... 36 1.1
UniRef50_Q2UES6 Cluster: Predicted protein; n=6; Trichocomaceae|... 36 1.4
UniRef50_Q8R7R6 Cluster: Malic enzyme; n=10; cellular organisms|... 35 2.5
UniRef50_Q0STR8 Cluster: Malate oxidoreductase; n=2; Clostridium... 35 2.5
UniRef50_Q2STW7 Cluster: Putative uncharacterized protein; n=1; ... 34 3.3
UniRef50_UPI00015555BF Cluster: PREDICTED: similar to leishmanol... 34 4.4
UniRef50_Q61829 Cluster: MOD-1 null malic enzyme; n=1; Mus muscu... 34 4.4
UniRef50_Q01PP2 Cluster: Heparinase II/III family protein; n=1; ... 34 4.4
UniRef50_UPI0000DD86B8 Cluster: PREDICTED: hypothetical protein;... 33 5.8
UniRef50_UPI0000DD8673 Cluster: PREDICTED: hypothetical protein;... 33 5.8
UniRef50_UPI0000D9B78D Cluster: PREDICTED: hypothetical protein;... 33 5.8
UniRef50_UPI00006CF21C Cluster: Bowman-Birk serine protease inhi... 33 7.7
UniRef50_Q6A639 Cluster: Conserved protein; n=3; Actinomycetales... 33 7.7
UniRef50_Q9FB23 Cluster: Peptide synthetase NRPS5-4-3; n=1; Stre... 33 7.7
UniRef50_Q2CHG8 Cluster: Type I secretion target repeat protein;... 33 7.7
UniRef50_A0UI99 Cluster: Putative uncharacterized protein; n=1; ... 33 7.7
UniRef50_Q7XBC7 Cluster: Putative transposase protein; n=2; Zea ... 33 7.7
UniRef50_A0DQ15 Cluster: Chromosome undetermined scaffold_6, who... 33 7.7
UniRef50_A6RMY7 Cluster: Predicted protein; n=1; Botryotinia fuc... 33 7.7
UniRef50_Q92797 Cluster: Symplekin; n=89; Eukaryota|Rep: Symplek... 33 7.7
UniRef50_P16468 Cluster: NAD-dependent malic enzyme; n=32; Bacte... 33 7.7
>UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 365
Score = 142 bits (345), Expect = 6e-33
Identities = 60/84 (71%), Positives = 71/84 (84%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ D++ NWP T+V+AIV+TDGERILGLGDLG CGMGIPVGKLALYT GGI P CLP+
Sbjct: 132 HIRDIVSNWPTTEVKAIVMTDGERILGLGDLGCCGMGIPVGKLALYTVCGGIDPEGCLPV 191
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +LDDP YIG+RQ+R
Sbjct: 192 MIDVGTNNEELLDDPFYIGVRQKR 215
Score = 45.6 bits (103), Expect = 0.001
Identities = 20/37 (54%), Positives = 29/37 (78%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNRPEEDLT 657
V+AMK EG E++A+ +IW+VDS+GL+VK+R LT
Sbjct: 252 VLAMKKEGLTEEQAKKKIWLVDSRGLVVKDRDCGGLT 288
>UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 613
Score = 139 bits (337), Expect = 6e-32
Identities = 62/83 (74%), Positives = 72/83 (86%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
V DVL+NWPE +++ IVVTDGERILGLGDLG GMGIPVGKL+LYTALGG++P CLPIT
Sbjct: 218 VLDVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPIT 277
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L+D YIGLRQRR
Sbjct: 278 IDVGTNNEQLLNDEFYIGLRQRR 300
Score = 73.3 bits (172), Expect = 6e-12
Identities = 35/82 (42%), Positives = 48/82 (58%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y EL++EFM AV + YG+ L+QFEDF N NAF LL KY + FNDDIQ
Sbjct: 303 GKEYHELMEEFMSAVKQIYGEKVLIQFEDFANHNAFDLLAKYSKSHLVFNDDIQGTASVV 362
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
SL++ L+++ +F
Sbjct: 363 LAGLLSSLKVVGGTLAEHTYLF 384
Score = 34.7 bits (76), Expect = 2.5
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 574 PEQEARCRIWMVDSKGLIVKNRPEEDLTFTR 666
P +E R ++W++DSKGLIV +R E F +
Sbjct: 409 PIEECRKKVWLLDSKGLIVNSRKESLQAFKK 439
Score = 34.7 bits (76), Expect = 2.5
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
L K+ +A +H PV TL + V+ +P+VLIG + +
Sbjct: 434 LQAFKKPWAHEHEPVTTLLDAVQSIKPTVLIGTSGV 469
>UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52;
cellular organisms|Rep: NADP-dependent malic enzyme -
Mus musculus (Mouse)
Length = 572
Score = 136 bits (329), Expect = 6e-31
Identities = 62/84 (73%), Positives = 68/84 (80%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ VL WPE V+AIVVTDGERILGLGDLG GMGIPVGKLALYTA GG+ P QCLPI
Sbjct: 132 HIASVLNAWPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPI 191
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
T+DVGT + +L DPLYIGLR RR
Sbjct: 192 TLDVGTENEELLKDPLYIGLRHRR 215
Score = 116 bits (278), Expect = 8e-25
Identities = 50/85 (58%), Positives = 60/85 (70%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RGP+YD LDEFM A +YG NCL+QFEDF N NAFRLL KYRNKYCTFNDDIQ
Sbjct: 216 VRGPEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTAS 275
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVFQ 508
+LR+T+ +LSD ++FQ
Sbjct: 276 VAVAGLLAALRITKNKLSDQTVLFQ 300
Score = 44.8 bits (101), Expect = 0.002
Identities = 20/31 (64%), Positives = 25/31 (80%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
VMAM+ EG ++ AR +IW+VDSKGLIVK R
Sbjct: 314 VMAMEKEGLSKENARKKIWLVDSKGLIVKGR 344
Score = 33.9 bits (74), Expect = 4.4
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
L KE FA +H ++ L +V+ +P+ LIG AAI
Sbjct: 347 LTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAI 382
>UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63;
Eukaryota|Rep: NADP-dependent malic enzyme - Homo
sapiens (Human)
Length = 572
Score = 133 bits (321), Expect = 5e-30
Identities = 59/84 (70%), Positives = 68/84 (80%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ VL WPE ++AIVVTDGERILGLGDLG GMGIPVGKLALYTA GG+ P +CLP+
Sbjct: 132 HIASVLNAWPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPV 191
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+DVGT + +L DPLYIGLRQRR
Sbjct: 192 ILDVGTENEELLKDPLYIGLRQRR 215
Score = 114 bits (275), Expect = 2e-24
Identities = 50/85 (58%), Positives = 61/85 (71%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RG +YD+ LDEFM AV +YG NCL+QFEDF N NAFRLL KYRN+YCTFNDDIQ
Sbjct: 216 VRGSEYDDFLDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTAS 275
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVFQ 508
+LR+T+ +LSD I+FQ
Sbjct: 276 VAVAGLLAALRITKNKLSDQTILFQ 300
Score = 44.0 bits (99), Expect = 0.004
Identities = 19/31 (61%), Positives = 26/31 (83%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
VMA++ EG P+++A +IW+VDSKGLIVK R
Sbjct: 314 VMALEKEGLPKEKAIKKIWLVDSKGLIVKGR 344
Score = 35.9 bits (79), Expect = 1.1
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
L KE+FA +H ++ L +V+ +P+ LIG AAI
Sbjct: 347 LTQEKEKFAHEHEEMKNLEAIVQEIKPTALIGVAAI 382
>UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondrial
precursor; n=15; Bilateria|Rep: NADP-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 604
Score = 132 bits (320), Expect = 7e-30
Identities = 56/84 (66%), Positives = 70/84 (83%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ +L +WPE +++A+VVTDGERILGLGDLG GMGIPVGKLALYTA GG+ P QCLP+
Sbjct: 167 HLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPV 226
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+DVGTN + +L DPLYIGL+ +R
Sbjct: 227 LLDVGTNNEELLRDPLYIGLKHQR 250
Score = 110 bits (265), Expect = 3e-23
Identities = 50/85 (58%), Positives = 61/85 (71%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+ G YD+LLDEFM+AV ++G NCL+QFEDF NANAFRLL KYRNKYC FNDDIQ
Sbjct: 251 VHGKAYDDLLDEFMQAVTDKFGINCLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTAS 310
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVFQ 508
+LR+T +LS++V VFQ
Sbjct: 311 VAVAGILAALRITNNKLSNHVFVFQ 335
Score = 46.8 bits (106), Expect = 6e-04
Identities = 21/31 (67%), Positives = 25/31 (80%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
VMA++ EG P+ EA +IWMVDSKGLIVK R
Sbjct: 349 VMALEKEGVPKAEATRKIWMVDSKGLIVKGR 379
Score = 44.0 bits (99), Expect = 0.004
Identities = 20/36 (55%), Positives = 25/36 (69%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
LN KE FAQDH V +L EVV + +P+ +IG AAI
Sbjct: 382 LNHEKEMFAQDHPEVNSLEEVVRLVKPTAIIGVAAI 417
>UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplast
precursor; n=79; Magnoliophyta|Rep: NADP-dependent malic
enzyme, chloroplast precursor - Zea mays (Maize)
Length = 636
Score = 131 bits (317), Expect = 2e-29
Identities = 59/83 (71%), Positives = 69/83 (83%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
V +VL+NWP +++ I VTDGERILGLGDLG GMGIPVGKLALYTALGG+ P CLPIT
Sbjct: 215 VLEVLRNWPHRNIQVICVTDGERILGLGDLGCQGMGIPVGKLALYTALGGVDPSVCLPIT 274
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L+D YIGLRQ+R
Sbjct: 275 IDVGTNNEFLLNDEFYIGLRQKR 297
Score = 77.4 bits (182), Expect = 4e-13
Identities = 36/82 (43%), Positives = 49/82 (59%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +YDEL++EFM AV + YG+ L+QFEDF N NAF LLEKY + FNDDIQ
Sbjct: 300 GEEYDELIEEFMSAVKQFYGEKVLIQFEDFANHNAFDLLEKYSKSHLVFNDDIQGTASVV 359
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+L++ L++ +F
Sbjct: 360 LAGLLAALKMVGGTLAEQTYLF 381
Score = 35.9 bits (79), Expect = 1.1
Identities = 14/38 (36%), Positives = 25/38 (65%)
Frame = +3
Query: 645 GGLNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
G L K+ +A +H P++TL + V+ +P+VLIG + +
Sbjct: 429 GSLQPFKKPWAHEHEPLKTLYDAVQSIKPTVLIGTSGV 466
Score = 34.3 bits (75), Expect = 3.3
Identities = 14/27 (51%), Positives = 19/27 (70%)
Frame = +1
Query: 559 KGEGTPEQEARCRIWMVDSKGLIVKNR 639
K P +E R ++W+VDSKGLIV +R
Sbjct: 401 KQTNAPIEECRKKVWLVDSKGLIVDSR 427
>UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole genome
shotgun sequence; n=3; Vitis vinifera|Rep: Chromosome
chr2 scaffold_11, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 573
Score = 130 bits (314), Expect = 4e-29
Identities = 57/83 (68%), Positives = 68/83 (81%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ +VLKNWPE ++ IVVT+GERILGLGDLG GMGIPVGKL+LYT LGG+ P CLP+T
Sbjct: 315 ILEVLKNWPERSIQVIVVTNGERILGLGDLGCQGMGIPVGKLSLYTTLGGLHPSVCLPVT 374
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L D YIGL+QRR
Sbjct: 375 IDVGTNNEQLLKDEFYIGLKQRR 397
Score = 33.9 bits (74), Expect = 4.4
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +3
Query: 663 KERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
K+ +A +H P+R L + V+ +P+VLIG+ I
Sbjct: 513 KKPWAHEHEPIRALVDAVQSIKPTVLIGSLGI 544
Score = 33.1 bits (72), Expect = 7.7
Identities = 14/24 (58%), Positives = 19/24 (79%)
Frame = +1
Query: 574 PEQEARCRIWMVDSKGLIVKNRPE 645
P +E R +IW+V+SKGLIV +R E
Sbjct: 484 PLEETRKKIWLVNSKGLIVHSRME 507
>UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=53; Eumetazoa|Rep: NAD-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 584
Score = 126 bits (304), Expect = 6e-28
Identities = 55/84 (65%), Positives = 67/84 (79%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
HV ++ NWPE V+A+VVTDGERILGLGDLG GMGIPVGKL LYTA GI+P +CLP+
Sbjct: 142 HVRSIVDNWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPV 201
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
IDVGT+ ++L DP Y+GL Q+R
Sbjct: 202 CIDVGTDNIALLKDPFYMGLYQKR 225
Score = 97.5 bits (232), Expect = 3e-19
Identities = 43/83 (51%), Positives = 56/83 (67%)
Frame = +2
Query: 257 RGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXX 436
R YD+L+DEFM+A+ RYG+N L+QFEDFGN NAFR L KYR KYCTFNDDIQ
Sbjct: 227 RTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAV 286
Query: 437 XXXXXXXSLRLTQKRLSDNVIVF 505
+ ++ K +S++ I+F
Sbjct: 287 ALAGLLAAQKVISKPISEHKILF 309
Score = 37.5 bits (83), Expect = 0.36
Identities = 17/31 (54%), Positives = 21/31 (67%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
VM+M G EQEA+ +IWM D GL+VK R
Sbjct: 324 VMSMVENGLSEQEAQKKIWMFDKYGLLVKGR 354
>UniRef50_A0L5P5 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Magnetococcus sp. MC-1|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Magnetococcus
sp. (strain MC-1)
Length = 556
Score = 124 bits (299), Expect = 2e-27
Identities = 56/83 (67%), Positives = 67/83 (80%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ ++L NW DVR IVVTDG RILGLGDLGA GMGIPVGKLALYTAL GI P CLP+T
Sbjct: 137 IAELLGNWVHKDVRVIVVTDGSRILGLGDLGAHGMGIPVGKLALYTALAGIPPIHCLPVT 196
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+D+GTN +++ +DPLY+GL Q R
Sbjct: 197 LDMGTNNEALRNDPLYVGLPQPR 219
Score = 76.6 bits (180), Expect = 6e-13
Identities = 37/88 (42%), Positives = 54/88 (61%)
Frame = +2
Query: 242 PAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
P + G YDE+++EF+ AV ++ + ++QFEDF N NAFRLLEKY+++ CTFNDDIQ
Sbjct: 216 PQPRVTGEIYDEMIEEFVLAVQEQFPR-AMIQFEDFSNDNAFRLLEKYQDRICTFNDDIQ 274
Query: 422 XXXXXXXXXXXXSLRLTQKRLSDNVIVF 505
+LR+T+ L +F
Sbjct: 275 GTAAVALAGILGALRITEADLCTQRYLF 302
>UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|Rep:
Malic enzyme - Bradyrhizobium japonicum
Length = 531
Score = 123 bits (297), Expect = 4e-27
Identities = 55/83 (66%), Positives = 67/83 (80%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ ++LKNWP R IVVTDGERILGLGDLGA GMGIPVGKL+LY+A G+ P CLPI
Sbjct: 118 IAEILKNWPYP-ARLIVVTDGERILGLGDLGANGMGIPVGKLSLYSACAGVHPEHCLPIV 176
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+DVGTN + +L+DP Y+GLR+RR
Sbjct: 177 LDVGTNNEELLNDPYYLGLRERR 199
Score = 71.7 bits (168), Expect = 2e-11
Identities = 37/84 (44%), Positives = 48/84 (57%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G YD +DEFM A R+ L+QFEDF N +AF+LL KYR++ C FNDDIQ
Sbjct: 200 LTGEAYDSFVDEFMVAA-RKTFPGVLIQFEDFANHSAFKLLHKYRDEACVFNDDIQGTAA 258
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
LR++ +L D I+F
Sbjct: 259 VALAGLFSGLRISGGKLKDQKILF 282
Score = 37.1 bits (82), Expect = 0.47
Identities = 17/31 (54%), Positives = 20/31 (64%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
V AM EG E EA R W+VDS+GL+V R
Sbjct: 297 VSAMMAEGATEAEALRRNWLVDSRGLVVSGR 327
>UniRef50_Q0AIF8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Nitrosomonas|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Nitrosomonas
eutropha (strain C71)
Length = 536
Score = 120 bits (290), Expect = 3e-26
Identities = 51/74 (68%), Positives = 63/74 (85%)
Frame = +3
Query: 18 LKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVG 197
LKNWPETDV+ IVVTDGERILGLGDLGA GMGIP+GK++LY A GI P +C+P+ +DVG
Sbjct: 128 LKNWPETDVQIIVVTDGERILGLGDLGANGMGIPIGKISLYVACAGIYPDRCMPVMLDVG 187
Query: 198 TNTQSMLDDPLYIG 239
T Q++ +DPLY+G
Sbjct: 188 TGNQALREDPLYLG 201
Score = 66.9 bits (156), Expect = 5e-10
Identities = 36/83 (43%), Positives = 45/83 (54%)
Frame = +2
Query: 242 PAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
P L G DY L+DEF+ AV RY + L+QFEDF + NAF LL++Y FNDDIQ
Sbjct: 203 PYPRLVGQDYLSLVDEFVEAVQHRY-PDALIQFEDFSSRNAFTLLDRYAASVRCFNDDIQ 261
Query: 422 XXXXXXXXXXXXSLRLTQKRLSD 490
S R+T K +D
Sbjct: 262 GTAAVTLAGIYASCRITHKSFAD 284
>UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic
enzyme - Mastigamoeba balamuthi (Phreatamoeba balamuthi)
Length = 568
Score = 116 bits (280), Expect = 5e-25
Identities = 52/84 (61%), Positives = 66/84 (78%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
HV ++L+NWP +V IVVTDG RILGLGDLG+ GMGIP+GKL LY A G +P + LP+
Sbjct: 152 HVAEILENWPH-EVEIIVVTDGGRILGLGDLGSNGMGIPIGKLHLYIACAGFRPDRTLPV 210
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN Q +LDDP+Y+G+R+ R
Sbjct: 211 MIDVGTNRQELLDDPMYLGVRKAR 234
Score = 56.0 bits (129), Expect = 9e-07
Identities = 32/86 (37%), Positives = 47/86 (54%)
Frame = +2
Query: 248 AALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXX 427
A L +Y LL+EFM AV ++ + CLVQFEDF N F+LL+ + + FNDDIQ
Sbjct: 233 ARLGDAEYFALLEEFMTAVRAKWPR-CLVQFEDFPNPRCFQLLDTWFGRQFCFNDDIQGT 291
Query: 428 XXXXXXXXXXSLRLTQKRLSDNVIVF 505
++R T ++D+ +F
Sbjct: 292 GAVILAGVINAVRATGVPVADHRFMF 317
>UniRef50_A7CWP9 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Opitutaceae bacterium TAV2|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Opitutaceae
bacterium TAV2
Length = 561
Score = 116 bits (279), Expect = 6e-25
Identities = 50/83 (60%), Positives = 65/83 (78%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ ++L++WP TDVR IVVTDGERILGLGDLG GMGIPVGKLALY+A G+ P CLPI
Sbjct: 142 IAEILRHWPITDVRMIVVTDGERILGLGDLGVLGMGIPVGKLALYSACAGLHPSYCLPIA 201
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+D G + +++ +DPLY+G +R
Sbjct: 202 LDAGIDNETLRNDPLYLGHPMKR 224
Score = 73.7 bits (173), Expect = 4e-12
Identities = 36/88 (40%), Positives = 53/88 (60%)
Frame = +2
Query: 242 PAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
P +RG DYD L+EF+ AV + + ++Q+EDFGN NAFRLL + R++ C+FNDDIQ
Sbjct: 221 PMKRVRGADYDAFLNEFVEAVKTVFPKT-MIQWEDFGNTNAFRLLHENRSRVCSFNDDIQ 279
Query: 422 XXXXXXXXXXXXSLRLTQKRLSDNVIVF 505
+L T +L+D ++F
Sbjct: 280 GTAAVALAGVISALNSTGGKLADQKLLF 307
Score = 40.7 bits (91), Expect = 0.038
Identities = 17/32 (53%), Positives = 22/32 (68%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNRP 642
V A + G E++AR R W VDS+GL+VK RP
Sbjct: 322 VAAAREAGLSEEQARARCWFVDSQGLVVKTRP 353
Score = 38.7 bits (86), Expect = 0.15
Identities = 19/37 (51%), Positives = 24/37 (64%)
Frame = +3
Query: 642 GGGLNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAA 752
G L HK +A D A V TLAE VEV +P++LIG +
Sbjct: 354 GRALAHHKLPYAHDAAHVATLAEAVEVVKPTILIGVS 390
>UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila
melanogaster|Rep: Malic enzyme - Drosophila melanogaster
(Fruit fly)
Length = 610
Score = 116 bits (279), Expect = 6e-25
Identities = 55/85 (64%), Positives = 64/85 (75%), Gaps = 1/85 (1%)
Frame = +3
Query: 3 HVYDVLKNWP-ETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLP 179
H+ +L NWP V+AI VTDG+RILGLGDLGA GMGI VGK+ LYTAL GI P LP
Sbjct: 163 HMKQILSNWPMRRSVKAICVTDGQRILGLGDLGANGMGIAVGKMELYTALAGIPPSMLLP 222
Query: 180 ITIDVGTNTQSMLDDPLYIGLRQRR 254
I +DVGTN +S+ +DPLYIGLR R
Sbjct: 223 ICLDVGTNNKSLHEDPLYIGLRDER 247
Score = 77.4 bits (182), Expect = 4e-13
Identities = 37/84 (44%), Positives = 49/84 (58%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L+G +Y +DEFM AVV +G L+ FEDF NAF L +Y++ YC FNDDIQ
Sbjct: 248 LKGDEYVCFVDEFMEAVVSTFGDQTLIHFEDFATPNAFMFLNRYQHCYCHFNDDIQGTAA 307
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
R+T+K L ++VIVF
Sbjct: 308 VGLGGLLGIQRITKKPLEEHVIVF 331
>UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:
Malic enzyme - Botryotinia fuckeliana B05.10
Length = 685
Score = 112 bits (270), Expect = 8e-24
Identities = 51/81 (62%), Positives = 61/81 (75%)
Frame = +3
Query: 12 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 191
+VL NW ++DV VVTDG RILGLGDLG GMGIPVGKL+LYT GI P + LPIT+D
Sbjct: 210 EVLGNWRQSDVEMTVVTDGSRILGLGDLGVNGMGIPVGKLSLYTGCAGIHPSKTLPITLD 269
Query: 192 VGTNTQSMLDDPLYIGLRQRR 254
+GTN + L DPLY+G R +R
Sbjct: 270 LGTNNEKFLKDPLYMGNRMKR 290
Score = 45.6 bits (103), Expect = 0.001
Identities = 29/77 (37%), Positives = 39/77 (50%)
Frame = +2
Query: 275 ELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXXXXX 454
E ++E M A+ + + +VQ+EDF N F L KY+NKY FNDDIQ
Sbjct: 298 EFMEELMVALNKMW-PGIIVQYEDF--KNPFPSLAKYQNKYPCFNDDIQGTGAVILAGII 354
Query: 455 XSLRLTQKRLSDNVIVF 505
+LR T + D VF
Sbjct: 355 NALRRTGVPVKDQRAVF 371
>UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP+-dependent malic enzyme -
Ostreococcus tauri
Length = 580
Score = 110 bits (265), Expect = 3e-23
Identities = 50/84 (59%), Positives = 63/84 (75%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
+V +++NWP TDV+ V+TDGERILGLGD GA GMGI GK +Y A G+ P LPI
Sbjct: 163 NVKQLIRNWPATDVKIAVITDGERILGLGDQGANGMGISAGKSMVYAAC-GVPPSALLPI 221
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+D GTN Q++LDDPLYIGL+Q+R
Sbjct: 222 QVDTGTNNQTLLDDPLYIGLKQKR 245
Score = 55.6 bits (128), Expect = 1e-06
Identities = 26/53 (49%), Positives = 36/53 (67%), Gaps = 2/53 (3%)
Frame = +2
Query: 269 YDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRN--KYCTFNDDIQ 421
YD LLDE + AV RYGQ ++ +EDF NAFR+L +++ + T+NDDIQ
Sbjct: 251 YDALLDEIVVAVRSRYGQKTIIHWEDFAPKNAFRVLRRFQQAPEVVTYNDDIQ 303
Score = 38.7 bits (86), Expect = 0.15
Identities = 15/34 (44%), Positives = 24/34 (70%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNRPEE 648
V A+ G E+EA+ R+W+ DSKGL+V++R +
Sbjct: 346 VSALVQRGVSEEEAKKRVWLFDSKGLVVRSRASQ 379
>UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor
circinelloides|Rep: Malic enzyme - Mucor circinelloides
Length = 617
Score = 110 bits (265), Expect = 3e-23
Identities = 48/77 (62%), Positives = 58/77 (75%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
NWP V IVVTDG R+LGLGDLGA GM IP+GKL+LY A GGI+P LP+ +DVGTN
Sbjct: 174 NWPHDKVDVIVVTDGSRVLGLGDLGANGMEIPIGKLSLYVAAGGIRPRAVLPVVLDVGTN 233
Query: 204 TQSMLDDPLYIGLRQRR 254
Q +L+DPLY+G+ R
Sbjct: 234 NQDLLNDPLYLGMSHPR 250
Score = 57.6 bits (133), Expect = 3e-07
Identities = 32/86 (37%), Positives = 49/86 (56%), Gaps = 3/86 (3%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G +Y +DE++ A+ R+ + L+QFEDF +A+ LL KYRN+ FNDDIQ
Sbjct: 251 LEGEEYYSFIDEWVTAITSRW-PDTLIQFEDFKYPHAYNLLAKYRNRITCFNDDIQSTSS 309
Query: 434 XXXXXXXXSLR---LTQKRLSDNVIV 502
SL+ ++Q+ LS+ I+
Sbjct: 310 ITLAGILASLKSRGMSQEDLSNERII 335
>UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 694
Score = 110 bits (264), Expect = 4e-23
Identities = 59/124 (47%), Positives = 72/124 (58%), Gaps = 10/124 (8%)
Frame = +2
Query: 80 GPGRFGRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHP--VDAG--------RPA 229
G G G G P G+ LHR RR A++V RG+ P V G P
Sbjct: 205 GLGDLGSYGMGIPVGKLALLHRLRRRAAAAVPPGAAGRGHRQPGSVPGGVCVQVLLDDPL 264
Query: 230 VHRAPAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFN 409
+RG DYD L++EFM+AV +YG NCL+QFEDF N+NAFR+L KYRN+YCTFN
Sbjct: 265 YIGLKHKRVRGKDYDGLIEEFMQAVTDKYGMNCLIQFEDFANSNAFRILNKYRNRYCTFN 324
Query: 410 DDIQ 421
DDIQ
Sbjct: 325 DDIQ 328
Score = 91.9 bits (218), Expect = 2e-17
Identities = 48/94 (51%), Positives = 61/94 (64%), Gaps = 10/94 (10%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALY-----TALGGIKPH 167
H+ +L +WPE D++A+VVTDGERILGLGDLG+ GMGIPVGKLAL A + P
Sbjct: 179 HIATMLNSWPEEDIKAVVVTDGERILGLGDLGSYGMGIPVGKLALLHRLRRRAAAAVPPG 238
Query: 168 QC-----LPITIDVGTNTQSMLDDPLYIGLRQRR 254
P ++ G Q +LDDPLYIGL+ +R
Sbjct: 239 AAGRGHRQPGSVPGGVCVQVLLDDPLYIGLKHKR 272
Score = 44.4 bits (100), Expect = 0.003
Identities = 20/31 (64%), Positives = 24/31 (77%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
+MAM EG ++EA RIWMVDS+GLIVK R
Sbjct: 410 IMAMAKEGLSKEEAARRIWMVDSRGLIVKGR 440
Score = 41.1 bits (92), Expect = 0.029
Identities = 18/36 (50%), Positives = 24/36 (66%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
LN KE FA DH +RTL EVV RP+ +IG +++
Sbjct: 443 LNHEKEEFAHDHPHLRTLEEVVHTIRPTAIIGKSSL 478
>UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;
n=1; Neocallimastix frontalis|Rep: Malic enzyme,
hydrogenosomal precursor - Neocallimastix frontalis
(Rumen fungus)
Length = 592
Score = 109 bits (263), Expect = 6e-23
Identities = 50/80 (62%), Positives = 58/80 (72%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
V NWP DV IVVTDG RILGLGDLGA GM IP+GKL LY GGI P LPI +DV
Sbjct: 168 VAANWPYDDVDVIVVTDGSRILGLGDLGAGGMQIPIGKLTLYVCGGGINPRNVLPIVLDV 227
Query: 195 GTNTQSMLDDPLYIGLRQRR 254
GTN + +L+DPLY+G++ R
Sbjct: 228 GTNNKELLNDPLYLGMQHPR 247
Score = 53.2 bits (122), Expect = 7e-06
Identities = 24/56 (42%), Positives = 38/56 (67%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
L+G ++ +DE++ A+ R+ + ++QFEDF NA LL KY+++ C FNDDIQ
Sbjct: 248 LQGEEFHAFVDEWVSAITDRFPK-AVIQFEDFMMPNALDLLLKYKDQICMFNDDIQ 302
>UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP dependent malic enzyme -
Ostreococcus tauri
Length = 641
Score = 109 bits (262), Expect = 7e-23
Identities = 48/83 (57%), Positives = 64/83 (77%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
VY +LKNWP +V+A+V+TDG+R+ G+GDLG GM V K +L+TALGG+ P LPI
Sbjct: 201 VYRLLKNWPVRNVKAVVLTDGQRVTGIGDLGVQGMPAAVSKASLFTALGGLDPADVLPIC 260
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGT+ Q++L+D YIGLRQ+R
Sbjct: 261 IDVGTDNQTLLEDKFYIGLRQKR 283
Score = 53.6 bits (123), Expect = 5e-06
Identities = 24/54 (44%), Positives = 34/54 (62%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
G Y+ELLDE + + RR+G L+ FE+F NANA +LL +Y + DD+Q
Sbjct: 286 GEAYEELLDEVVSGLKRRFGPRVLLCFEEFSNANAKKLLSRYNRDSVAYVDDLQ 339
>UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 1023
Score = 109 bits (261), Expect = 1e-22
Identities = 49/80 (61%), Positives = 59/80 (73%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
+L+NWP VVTDG RILGLGDLG G+GIP+GKLALYTA GI P + LPI +D
Sbjct: 593 ILRNWPYPQPEICVVTDGSRILGLGDLGVNGVGIPIGKLALYTAAAGIHPDKTLPIVLDC 652
Query: 195 GTNTQSMLDDPLYIGLRQRR 254
GT ++ L DPLY+GLRQ+R
Sbjct: 653 GTANETNLKDPLYLGLRQKR 672
Score = 54.0 bits (124), Expect = 4e-06
Identities = 27/80 (33%), Positives = 43/80 (53%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
+ E +DEFM A + + +VQFEDF + AF L++YR++Y FNDDIQ
Sbjct: 677 EQQEFMDEFMEAAAEVF-PDMVVQFEDFESEKAFNYLDRYRDQYKCFNDDIQGTGAVVLA 735
Query: 446 XXXXSLRLTQKRLSDNVIVF 505
++ L+ + + +VF
Sbjct: 736 GYIGAVNLSNVPIEEQRLVF 755
Score = 34.3 bits (75), Expect = 3.3
Identities = 12/27 (44%), Positives = 21/27 (77%)
Frame = +1
Query: 568 GTPEQEARCRIWMVDSKGLIVKNRPEE 648
G EQ+A+ + W+VD+KGL+ K+R ++
Sbjct: 777 GLSEQQAKDKFWLVDTKGLVTKDRGDK 803
>UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep:
Malic enzyme - Trichomonas vaginalis G3
Length = 567
Score = 108 bits (259), Expect = 2e-22
Identities = 48/83 (57%), Positives = 62/83 (74%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ D+L+N+P D+R IVVTD RILGLGDLGA G+GIPVGKL LYT +G + P Q LP+
Sbjct: 143 IKDILRNYPRQDIRCIVVTDAGRILGLGDLGASGLGIPVGKLMLYTLIGQVDPDQTLPVQ 202
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+D+GT+ + +L DPLY G R R
Sbjct: 203 LDMGTDRKEILADPLYHGWRHPR 225
Score = 56.0 bits (129), Expect = 9e-07
Identities = 27/66 (40%), Positives = 39/66 (59%)
Frame = +2
Query: 224 PAVHRAPAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCT 403
P H +RG ++ + + EF+ AV +G CLVQFEDF AF+LL+ +R +
Sbjct: 216 PLYHGWRHPRVRGAEHLKFVTEFVEAVKEVFGDTCLVQFEDFEMETAFKLLDHFRWRCNC 275
Query: 404 FNDDIQ 421
FNDDI+
Sbjct: 276 FNDDIE 281
>UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:
Malic enzyme - Cryptosporidium parvum Iowa II
Length = 614
Score = 107 bits (258), Expect = 2e-22
Identities = 46/83 (55%), Positives = 64/83 (77%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+Y++LK++ D+ IV+TDG RILGLGDLG GMGIP+GKL+LY LGGI P + LPI+
Sbjct: 188 IYEILKDFKSDDIEIIVLTDGGRILGLGDLGLNGMGIPMGKLSLYITLGGIDPSKVLPIS 247
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+D+GTNT +L D Y+G+ ++R
Sbjct: 248 LDIGTNTNDILSDKYYLGICEKR 270
Score = 49.6 bits (113), Expect = 8e-05
Identities = 25/79 (31%), Positives = 43/79 (54%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
+Y L+DE +A+ +R+ N ++Q+ED + A +L+ Y++K+ FNDDIQ
Sbjct: 275 EYFPLMDEITKAIFKRW-PNTVLQWEDLTTSRAIDILDIYQDKFRCFNDDIQGTASIVLA 333
Query: 446 XXXXSLRLTQKRLSDNVIV 502
SL++ K D I+
Sbjct: 334 GLVSSLKIAGKNFEDQRIL 352
Score = 33.5 bits (73), Expect = 5.8
Identities = 14/31 (45%), Positives = 21/31 (67%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
+ +M+ +G P Q A + W+VDSKGLI +R
Sbjct: 368 IKSMEIKGIPYQSALEKFWLVDSKGLITNSR 398
>UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;
Pezizomycotina|Rep: NADP-dependent malic enzyme MaeA -
Aspergillus fumigatus (Sartorya fumigata)
Length = 661
Score = 107 bits (258), Expect = 2e-22
Identities = 45/78 (57%), Positives = 61/78 (78%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
V+ NWP+ +V +TDG RILGLGDLG GMGIP+GKLALYTA GI+P LP+T+D+
Sbjct: 203 VIANWPQPNVEITCITDGSRILGLGDLGINGMGIPIGKLALYTACAGIRPEATLPLTLDL 262
Query: 195 GTNTQSMLDDPLYIGLRQ 248
GT+ +++ +DPLY+G R+
Sbjct: 263 GTSNKALREDPLYMGTRR 280
Score = 47.6 bits (108), Expect = 3e-04
Identities = 24/49 (48%), Positives = 31/49 (63%)
Frame = +2
Query: 275 ELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
E +DE M A+ R+ ++QFEDF N F LE+YR+ Y FNDDIQ
Sbjct: 290 EFMDELMAALTERW-PGIVIQFEDF--KNPFPALERYRDAYTCFNDDIQ 335
>UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 603
Score = 106 bits (255), Expect = 5e-22
Identities = 45/81 (55%), Positives = 61/81 (75%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ D++ NW + V+AI VTDG R+LGLGD+GA MGI +GK+ LYTALG I P +P+
Sbjct: 155 HIRDLMHNWVDEGVKAICVTDGGRVLGLGDMGANAMGISLGKMILYTALGSIPPSTLMPV 214
Query: 183 TIDVGTNTQSMLDDPLYIGLR 245
+DVGT+ Q++L DPLY+G R
Sbjct: 215 CLDVGTDNQALLQDPLYVGAR 235
Score = 88.2 bits (209), Expect = 2e-16
Identities = 38/84 (45%), Positives = 52/84 (61%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
++GP+YDEL+DEFM + V+ +G N + FEDF NA + LEKY+ KYC FNDDIQ
Sbjct: 239 VKGPEYDELVDEFMESAVKCFGNNTFIHFEDFATPNALKFLEKYQYKYCCFNDDIQGTGA 298
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
R+T ++L D V +F
Sbjct: 299 TGLAAFINVERITGRKLEDTVFLF 322
>UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187;
cellular organisms|Rep: NAD-dependent malic enzyme -
Vibrio vulnificus
Length = 562
Score = 105 bits (253), Expect = 9e-22
Identities = 50/81 (61%), Positives = 58/81 (71%)
Frame = +3
Query: 12 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 191
D+L N +V+ IVVTDGERILGLGD G GMGIP+GKL+LYTA GGI P LPI +D
Sbjct: 134 DILNNASNHNVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPIVLD 193
Query: 192 VGTNTQSMLDDPLYIGLRQRR 254
VGTN L DP+Y+G R R
Sbjct: 194 VGTNNPQRLADPMYMGWRHPR 214
Score = 70.9 bits (166), Expect = 3e-11
Identities = 34/84 (40%), Positives = 49/84 (58%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+ G DYD ++EF++AV RR+ + L+QFEDF NA LLE+Y+N+ C FNDDIQ
Sbjct: 215 ITGADYDAFVEEFIQAVQRRW-PDALIQFEDFAQKNAMPLLERYKNRICCFNDDIQGTAA 273
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
+ + +LS+ I F
Sbjct: 274 VTVGSLMAACQAAGSKLSEQRITF 297
>UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 635
Score = 105 bits (252), Expect = 1e-21
Identities = 47/77 (61%), Positives = 56/77 (72%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
NWP V IVVTDG RILGLGDLG G+GI +GKL LY A GI P + LP+ IDVGTN
Sbjct: 217 NWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTN 276
Query: 204 TQSMLDDPLYIGLRQRR 254
+ +L DPLY+GL++ R
Sbjct: 277 NEKLLKDPLYLGLQEHR 293
Score = 63.3 bits (147), Expect = 6e-09
Identities = 30/56 (53%), Positives = 39/56 (69%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
L G +Y ++DEFM AV R+ N +VQFEDF + AFRLL++YR Y FNDD+Q
Sbjct: 294 LEGEEYVAIIDEFMEAVFARW-PNVIVQFEDFQSKWAFRLLQRYRKTYRMFNDDVQ 348
>UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus
californicus|Rep: Malic enzyme - Tigriopus californicus
(Marine copepod)
Length = 322
Score = 104 bits (249), Expect = 3e-21
Identities = 44/77 (57%), Positives = 57/77 (74%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
NW +V A+V+TDG RILGLGDLG G+GI +GKL LY A GG P + LP+ +D+GTN
Sbjct: 188 NWESDEVDAVVITDGSRILGLGDLGLGGLGISIGKLDLYVAAGGFHPRRVLPVVLDIGTN 247
Query: 204 TQSMLDDPLYIGLRQRR 254
Q +L+DP Y+GL+Q R
Sbjct: 248 NQKLLNDPNYLGLKQPR 264
Score = 51.6 bits (118), Expect = 2e-05
Identities = 24/54 (44%), Positives = 36/54 (66%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
G +Y ++DE M A R+ + L+Q EDF + +A LL++YR++Y FNDDIQ
Sbjct: 267 GEEYYAIMDELMAATKLRWPR-ALIQHEDFXSKHAVNLLKRYRSEYLMFNDDIQ 319
>UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isoform,
mitochondrial precursor; n=41; Eukaryota|Rep:
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor - Solanum tuberosum (Potato)
Length = 626
Score = 102 bits (245), Expect = 8e-21
Identities = 46/77 (59%), Positives = 56/77 (72%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
NWP V IVVTDG RILGLGDLG G+GI +GKL LY A GI P + LP+ IDVGT+
Sbjct: 183 NWPADQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTD 242
Query: 204 TQSMLDDPLYIGLRQRR 254
+++L DPLY+GL+ R
Sbjct: 243 NENLLKDPLYLGLQDHR 259
Score = 64.5 bits (150), Expect = 3e-09
Identities = 31/56 (55%), Positives = 41/56 (73%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
L G +Y E++DEFM AV R+ + +VQFEDF + AF+LL++YRN Y FNDDIQ
Sbjct: 260 LDGEEYIEVIDEFMEAVFTRW-PHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDIQ 314
>UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 600
Score = 102 bits (244), Expect = 1e-20
Identities = 45/68 (66%), Positives = 55/68 (80%)
Frame = +3
Query: 51 IVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPL 230
+VVTDG RILGLGDLG GMGI VGKL LY A GG+ PH CLP+ +D+GTN +++ +DPL
Sbjct: 187 LVVTDGSRILGLGDLGIGGMGISVGKLNLYVAGGGVNPHGCLPVVLDMGTNNEAVRNDPL 246
Query: 231 YIGLRQRR 254
YIGL+Q R
Sbjct: 247 YIGLKQPR 254
Score = 41.9 bits (94), Expect = 0.017
Identities = 20/49 (40%), Positives = 29/49 (59%)
Frame = +2
Query: 275 ELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
E +D FM A + + ++Q EDF + AF L +Y+ KY FNDDI+
Sbjct: 262 EFMDAFMAAASEAFPK-AVIQHEDFYSEAAFDFLARYKEKYRMFNDDIE 309
Score = 33.9 bits (74), Expect = 4.4
Identities = 13/27 (48%), Positives = 18/27 (66%)
Frame = +1
Query: 565 EGTPEQEARCRIWMVDSKGLIVKNRPE 645
+G E EAR R W++D+KGLI R +
Sbjct: 362 QGLTEDEARERFWLIDTKGLITSTRAD 388
>UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 498
Score = 100 bits (240), Expect = 3e-20
Identities = 46/84 (54%), Positives = 59/84 (70%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H++ + + V IV+TDG RILGLGDLG G+GIP+GKL +Y A GI P + LPI
Sbjct: 28 HIWSDIGSXDLCQVDMIVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPI 87
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+DVGTN Q +L+D LY+GLRQ R
Sbjct: 88 MLDVGTNNQRLLEDRLYLGLRQPR 111
Score = 44.4 bits (100), Expect = 0.003
Identities = 22/54 (40%), Positives = 29/54 (53%)
Frame = +2
Query: 335 QFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXXXXXXSLRLTQKRLSDNV 496
QFEDF AF L++YR K+C FNDDIQ ++R + L+D V
Sbjct: 167 QFEDFQMKWAFETLQRYRKKFCMFNDDIQGTAGVALAGLLGTVRAQGRPLTDFV 220
>UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 539
Score = 100 bits (240), Expect = 3e-20
Identities = 46/71 (64%), Positives = 53/71 (74%)
Frame = +3
Query: 42 VRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLD 221
V VVTDGERILGLGDLGA GMGI GK+ LYT G++P CLP+ +DVGTN Q +LD
Sbjct: 124 VAVAVVTDGERILGLGDLGAGGMGISEGKILLYTVCAGVRPSACLPVCLDVGTNNQRLLD 183
Query: 222 DPLYIGLRQRR 254
DP Y GLR+ R
Sbjct: 184 DPNYKGLRRTR 194
Score = 86.2 bits (204), Expect = 8e-16
Identities = 44/84 (52%), Positives = 53/84 (63%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L+G YD L+DEFMR + R + CLVQFEDFGNANAFR+LEKYR FNDDIQ
Sbjct: 195 LKGEAYDALVDEFMREM-RAWQPRCLVQFEDFGNANAFRILEKYRTTGPCFNDDIQGTAA 253
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
SLR T+ +L D ++F
Sbjct: 254 ITLAALLSSLRATKGKLEDQKVLF 277
>UniRef50_A7IMB8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Alphaproteobacteria|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Xanthobacter
sp. (strain Py2)
Length = 550
Score = 100 bits (239), Expect = 4e-20
Identities = 41/79 (51%), Positives = 57/79 (72%)
Frame = +3
Query: 12 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 191
+VL+NWP+ D+R + V+ G RILGLGD+GA GMGIP+GKL LYTA + P LP+ D
Sbjct: 139 EVLRNWPQKDIRFVCVSTGGRILGLGDIGANGMGIPIGKLQLYTACAAVPPDVLLPVLFD 198
Query: 192 VGTNTQSMLDDPLYIGLRQ 248
+GT+ + + DP Y+G R+
Sbjct: 199 IGTSNEHLRADPFYLGTRE 217
Score = 54.4 bits (125), Expect = 3e-06
Identities = 27/80 (33%), Positives = 45/80 (56%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
+ DEL +EF++AV + C V FED+ +A R+L++Y++K +NDDIQ
Sbjct: 224 ELDELTEEFVQAVNEVF-PGCCVHFEDWKGTDAIRMLDRYKDKILCYNDDIQGTASVALA 282
Query: 446 XXXXSLRLTQKRLSDNVIVF 505
+++ T +L D I+F
Sbjct: 283 GIAAAMKQTGGKLKDQRILF 302
>UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20;
Trypanosomatidae|Rep: Malic enzyme, putative -
Leishmania major
Length = 573
Score = 99.1 bits (236), Expect = 1e-19
Identities = 42/80 (52%), Positives = 62/80 (77%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
+++N +T++ IV+TDG RILGLGDLG+ G+GI +GK +LY A GG+KP + LP+ +DV
Sbjct: 140 LIQNLRKTNIDVIVITDGSRILGLGDLGSNGIGISIGKCSLYVAAGGVKPSRVLPVVMDV 199
Query: 195 GTNTQSMLDDPLYIGLRQRR 254
GTN + ++PLY+GLR+ R
Sbjct: 200 GTNNLELRNNPLYLGLRKPR 219
Score = 60.9 bits (141), Expect = 3e-08
Identities = 33/80 (41%), Positives = 46/80 (57%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
D+ LLDEFM AV + + +VQFEDF N + F +LE+Y+ KY FNDDIQ
Sbjct: 224 DFYALLDEFMEAVKDTW-PSAVVQFEDFSNNHCFDMLERYQKKYRCFNDDIQGTGAVIAA 282
Query: 446 XXXXSLRLTQKRLSDNVIVF 505
+++L++ L IVF
Sbjct: 283 GFHTAVKLSKIPLEQQRIVF 302
>UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,
partial; n=1; Macaca mulatta|Rep: PREDICTED: similar to
Y48B6A.12, partial - Macaca mulatta
Length = 456
Score = 97.5 bits (232), Expect = 3e-19
Identities = 43/72 (59%), Positives = 54/72 (75%)
Frame = +3
Query: 39 DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSML 218
D IVVTDG RILGLGDLG GMGIP+GKL+LY A GI P + +P+T+DVGTN +L
Sbjct: 136 DADIIVVTDGSRILGLGDLGTNGMGIPIGKLSLYVACAGINPGRTVPVTLDVGTNNPDLL 195
Query: 219 DDPLYIGLRQRR 254
+D +Y+G R +R
Sbjct: 196 NDDMYLGERHKR 207
Score = 60.5 bits (140), Expect = 4e-08
Identities = 32/82 (39%), Positives = 47/82 (57%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y +DEF+ AV R+ L+QFEDF N +AF LL++Y+ FNDDIQ
Sbjct: 210 GEEYYAAVDEFVDAVRSRW-PGVLIQFEDFTNDHAFPLLKRYQENILCFNDDIQGTGSVA 268
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
++R+ +++L D IVF
Sbjct: 269 LAGLVSAMRVKKEKLEDQRIVF 290
Score = 33.1 bits (72), Expect = 7.7
Identities = 15/36 (41%), Positives = 24/36 (66%), Gaps = 1/36 (2%)
Frame = +3
Query: 642 GGGLNVHKERFAQDHA-PVRTLAEVVEVARPSVLIG 746
GG + HK + +D A P+ +L EVV+ +P+VL+G
Sbjct: 336 GGNIQDHKVPYCRDDAEPMTSLLEVVKAIKPTVLLG 371
>UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:
Malic enzyme - Ustilago maydis (Smut fungus)
Length = 634
Score = 97.1 bits (231), Expect = 4e-19
Identities = 45/85 (52%), Positives = 58/85 (68%), Gaps = 2/85 (2%)
Frame = +3
Query: 6 VYDVLKNWPET--DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLP 179
+ +++NWP R V+TDG RILGLGDLG G GI +GKL+LY A GI P LP
Sbjct: 195 IASIVENWPVPAGSPRIAVITDGSRILGLGDLGWNGQGISIGKLSLYVAGAGIHPRATLP 254
Query: 180 ITIDVGTNTQSMLDDPLYIGLRQRR 254
I +D+GTN + L+DPLY+GLR+ R
Sbjct: 255 IVVDLGTNNKKNLEDPLYLGLRRER 279
Score = 62.9 bits (146), Expect = 8e-09
Identities = 29/52 (55%), Positives = 37/52 (71%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
+Y E +DE M A+ RY N ++QFEDF + NAFR LE+Y+ KY FNDDIQ
Sbjct: 284 EYIEFMDEVMHALHTRY-PNLIIQFEDFTSENAFRFLERYQGKYPMFNDDIQ 334
>UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2;
Mucoromycotina|Rep: Malic enzyme protein 2 - Mortierella
alpina (Mortierella renispora)
Length = 669
Score = 96.3 bits (229), Expect = 7e-19
Identities = 44/75 (58%), Positives = 53/75 (70%)
Frame = +3
Query: 30 PETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQ 209
P + V+TDG RILGLGDLG GMGIPVGKL LY A GI P + LPIT+D+GTN +
Sbjct: 235 PSLTPQIAVITDGSRILGLGDLGVGGMGIPVGKLQLYVAGAGIDPRRTLPITLDLGTNNE 294
Query: 210 SMLDDPLYIGLRQRR 254
L D Y+GLR++R
Sbjct: 295 DKLKDEFYLGLREKR 309
Score = 46.8 bits (106), Expect = 6e-04
Identities = 26/75 (34%), Positives = 40/75 (53%)
Frame = +2
Query: 281 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXXXXXXS 460
+D + A+ Y + L+QFEDF + +AF+LL+KY+ K FNDDIQ +
Sbjct: 319 VDAVIEALTSLYPK-LLIQFEDFSSEHAFQLLDKYQPKKFCFNDDIQGTGAVILAGFMNA 377
Query: 461 LRLTQKRLSDNVIVF 505
+ L D+ I+F
Sbjct: 378 INLAGIPAKDHKILF 392
Score = 37.9 bits (84), Expect = 0.27
Identities = 20/47 (42%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Frame = +1
Query: 568 GTPEQEARCRIWMVDSKGLIVKNR----PEEDLTFTRRDSLRTTRPS 696
G E EAR +W+VDSKGL+ +R E L F R+D+ + PS
Sbjct: 415 GMTEDEAREMVWLVDSKGLVTLDRGDKLAEHKLYFARKDNAGSQYPS 461
>UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=4;
Gammaproteobacteria|Rep: Malic enzyme aka malate
dehydrogenase (Oxaloacetate-decarboxylating) (NADP(+)) -
Psychromonas ingrahamii (strain 37)
Length = 571
Score = 95.1 bits (226), Expect = 2e-18
Identities = 39/72 (54%), Positives = 55/72 (76%)
Frame = +3
Query: 39 DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSML 218
D++ IVVTD + ILG+GD G GMGIP+GKL+LYT GI P C+PI +D+GT+ Q +L
Sbjct: 153 DIQIIVVTDSQGILGIGDQGVGGMGIPIGKLSLYTLGAGIHPDHCMPIALDIGTDNQDLL 212
Query: 219 DDPLYIGLRQRR 254
DP+Y+G+ ++R
Sbjct: 213 ADPMYLGIPKKR 224
Score = 40.7 bits (91), Expect = 0.038
Identities = 20/60 (33%), Positives = 32/60 (53%)
Frame = +2
Query: 242 PAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
P L G Y + F+ V + + ++Q+EDF +NAF L Y ++ +FNDD+Q
Sbjct: 221 PKKRLTGEPYKAFIKTFVEQVKLHFPK-AVLQWEDFSKSNAFDNLSDYEDELPSFNDDVQ 279
>UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2;
Aeromonas|Rep: NAD-dependent malic enzyme - Aeromonas
salmonicida (strain A449)
Length = 516
Score = 93.9 bits (223), Expect = 4e-18
Identities = 42/74 (56%), Positives = 55/74 (74%)
Frame = +3
Query: 33 ETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQS 212
E +V IV++DGER+LGLGDLG GMGI +GKLALY+A GGI P + LP+ +DVGTN
Sbjct: 121 EQEVDVIVISDGERVLGLGDLGIGGMGICIGKLALYSAAGGINPARTLPLCVDVGTNNPE 180
Query: 213 MLDDPLYIGLRQRR 254
+L+D Y+G + R
Sbjct: 181 LLEDDSYLGWQANR 194
Score = 49.6 bits (113), Expect = 8e-05
Identities = 23/54 (42%), Positives = 34/54 (62%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
G Y +D+ + A+ R+ Q ++QFEDF +A LL +YR++ C FNDDIQ
Sbjct: 197 GESYYHFMDKVVAAIRTRWPQ-VVLQFEDFAGKHAANLLARYRDELCMFNDDIQ 249
>UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus tauri
Length = 639
Score = 93.9 bits (223), Expect = 4e-18
Identities = 43/83 (51%), Positives = 57/83 (68%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ ++ NW + V IVVTDG RILGLGDLG GMGI GK+ LY A GG P LP+
Sbjct: 199 INSMMFNWKRS-VSVIVVTDGSRILGLGDLGTNGMGISQGKVDLYVAGGGFDPQHVLPVV 257
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+DVGTN + +L+DP Y+G+ ++R
Sbjct: 258 LDVGTNNEDLLNDPYYLGVPRKR 280
Score = 58.0 bits (134), Expect = 2e-07
Identities = 27/60 (45%), Positives = 38/60 (63%)
Frame = +2
Query: 242 PAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
P L G +Y ++EF+R V R+ N L+QFEDF +A ++L++YRN FNDDIQ
Sbjct: 277 PRKRLEGEEYYAFIEEFIRGVRNRW-PNALIQFEDFQTKHANKILQRYRNDVLCFNDDIQ 335
>UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme;
n=1; Desulfotalea psychrophila|Rep: Related to
NAD-dependent malic enzyme - Desulfotalea psychrophila
Length = 578
Score = 90.2 bits (214), Expect = 5e-17
Identities = 40/81 (49%), Positives = 57/81 (70%)
Frame = +3
Query: 12 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 191
D+L+ + D+R VVTD + ILG+GD GA G+ I +GKL LYT GI P CLPI++D
Sbjct: 145 DILRRFAHRDIRVAVVTDNQGILGIGDQGAGGIAICLGKLMLYTQGAGIAPWHCLPISLD 204
Query: 192 VGTNTQSMLDDPLYIGLRQRR 254
+GT+ +++L D Y+G RQ+R
Sbjct: 205 IGTDNEALLADKHYLGWRQKR 225
Score = 56.4 bits (130), Expect = 7e-07
Identities = 28/83 (33%), Positives = 44/83 (53%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G +Y +D F RA R N L Q+EDF NAF + +KYR+ +FNDD+Q
Sbjct: 226 LVGDEYTAFIDRFARAF-RNVFPNALCQWEDFSKQNAFDIRDKYRHSMVSFNDDVQGTGA 284
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
++++ + +L+D V +
Sbjct: 285 ITFSAILSAMKVKRAKLTDQVFL 307
>UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n=2;
Filobasidiella neoformans|Rep: Nad-dependent malic
enzyme, putative - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 584
Score = 89.4 bits (212), Expect = 8e-17
Identities = 38/72 (52%), Positives = 54/72 (75%)
Frame = +3
Query: 39 DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSML 218
++ IVV+DGE ILG+GD G+ G+GI K +Y+ + G+ P +CL IT+DVGTN Q +L
Sbjct: 158 ELELIVVSDGEAILGIGDQGSGGIGISGAKAVIYSLIAGVDPAKCLAITLDVGTNNQDLL 217
Query: 219 DDPLYIGLRQRR 254
+DPLYIG R++R
Sbjct: 218 NDPLYIGYREKR 229
Score = 37.5 bits (83), Expect = 0.36
Identities = 24/83 (28%), Positives = 40/83 (48%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
LR YD+ +D+F+ +V+++ CL+ FED + L + + FNDDIQ
Sbjct: 230 LRDDRYDQFVDKFV-GLVKKHQPECLLHFEDLVSP---MLNDSSEDIGARFNDDIQGTGA 285
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
++ +T+ L D IV
Sbjct: 286 VTLAAVQAAISVTKSSLKDQRIV 308
>UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2;
n=37; Bacteria|Rep: Probable NAD-dependent malic enzyme
2 - Bacillus subtilis
Length = 582
Score = 88.2 bits (209), Expect = 2e-16
Identities = 38/72 (52%), Positives = 51/72 (70%)
Frame = +3
Query: 39 DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSML 218
D+ IV TD E ILG+GD G G+ I +GKLA+YTA GI P + +P+ +DVGTN + +L
Sbjct: 166 DIDLIVATDSESILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLL 225
Query: 219 DDPLYIGLRQRR 254
+DPLYIG + R
Sbjct: 226 NDPLYIGNKHER 237
Score = 50.8 bits (116), Expect = 4e-05
Identities = 24/85 (28%), Positives = 48/85 (56%), Gaps = 1/85 (1%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
++G Y+ +D +++A ++ + + L+ +ED GN NA +++KY ++ TFNDDIQ
Sbjct: 238 VQGERYEAFIDAYVKAALKFFPK-ALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGA 296
Query: 434 XXXXXXXXSLRLTQKRLSD-NVIVF 505
+++ T + D V++F
Sbjct: 297 ITLAGVLAAMKKTGASIKDQRVVIF 321
>UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 306
Score = 87.8 bits (208), Expect = 3e-16
Identities = 38/66 (57%), Positives = 47/66 (71%)
Frame = +2
Query: 311 RYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXXXXXXSLRLTQKRLSD 490
RYG +CL+QFEDF N NAFRLL KYR+ YCTFNDDIQ +LR+T+ ++SD
Sbjct: 1 RYGMDCLIQFEDFANINAFRLLSKYRDMYCTFNDDIQGTAAVAVAGLLAALRITETKMSD 60
Query: 491 NVIVFQ 508
+ IVFQ
Sbjct: 61 HTIVFQ 66
Score = 49.6 bits (113), Expect = 8e-05
Identities = 23/30 (76%), Positives = 24/30 (80%)
Frame = +1
Query: 550 MAMKGEGTPEQEARCRIWMVDSKGLIVKNR 639
MAMK EG PEQE +IWMVDSKGLIVK R
Sbjct: 81 MAMKKEGLPEQECLKKIWMVDSKGLIVKGR 110
>UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 611
Score = 87.4 bits (207), Expect = 3e-16
Identities = 43/81 (53%), Positives = 55/81 (67%), Gaps = 2/81 (2%)
Frame = +3
Query: 18 LKNW--PETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITID 191
L W PE D+ IVVTDGE ILG+GD G G+ I + KL L T GGI P++ LP+ +D
Sbjct: 157 LAQWGKPE-DIDYIVVTDGEEILGIGDQGCGGILISIAKLVLMTICGGIHPNRVLPVVLD 215
Query: 192 VGTNTQSMLDDPLYIGLRQRR 254
GTN + +L D LY+GLRQ+R
Sbjct: 216 CGTNNEELLKDDLYLGLRQKR 236
Score = 58.8 bits (136), Expect = 1e-07
Identities = 31/84 (36%), Positives = 48/84 (57%), Gaps = 1/84 (1%)
Frame = +2
Query: 257 RGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXX 436
RG YD+ ++ F++A Y + + FEDFG NA RLLE+YR + FNDD+Q
Sbjct: 238 RGDKYDDFVETFIKAARELYPR-AYIHFEDFGLDNARRLLERYRPQMPCFNDDVQGTGCV 296
Query: 437 XXXXXXXSLRLTQKRLSD-NVIVF 505
L +++++LSD ++VF
Sbjct: 297 TLAAIMAGLHISKQKLSDVRMVVF 320
>UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6;
Deinococci|Rep: Malate oxidoreductase - Deinococcus
radiodurans
Length = 580
Score = 86.6 bits (205), Expect = 6e-16
Identities = 39/79 (49%), Positives = 51/79 (64%)
Frame = +3
Query: 18 LKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVG 197
L N P DVR IV TD ILG+GD G GM I +GKL+LYT GG+ P + LP+ +DVG
Sbjct: 159 LANVPLNDVRIIVATDSSAILGIGDQGFGGMAISIGKLSLYTVAGGVGPDKTLPVELDVG 218
Query: 198 TNTQSMLDDPLYIGLRQRR 254
T + +DP Y+G++ R
Sbjct: 219 TGRADLREDPHYLGVKHER 237
Score = 56.4 bits (130), Expect = 7e-07
Identities = 28/83 (33%), Positives = 45/83 (54%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G +Y +D+F+ A ++RY + ++Q+EDF AF +L +YR +FNDDIQ
Sbjct: 238 LTGEEYFAFIDKFVEATIQRYPK-AIIQWEDFSKDAAFEVLARYRKVVPSFNDDIQGTGA 296
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
+ R+ + L D V+V
Sbjct: 297 VVLAGVLNACRIKGEALKDQVVV 319
>UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep:
Lmo1915 protein - Listeria monocytogenes
Length = 547
Score = 85.4 bits (202), Expect = 1e-15
Identities = 39/78 (50%), Positives = 55/78 (70%)
Frame = +3
Query: 21 KNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGT 200
KN P D+ IV+TDGE +LG+GD G+ I VGKLA+YT G+ P + LP+ ID GT
Sbjct: 134 KNNPNIDM--IVITDGEGVLGIGDWSVNGVKIAVGKLAVYTVAAGLAPDRVLPVVIDAGT 191
Query: 201 NTQSMLDDPLYIGLRQRR 254
N +++L+DPLY+G ++ R
Sbjct: 192 NNETLLNDPLYLGNKRPR 209
Score = 56.8 bits (131), Expect = 5e-07
Identities = 27/79 (34%), Positives = 45/79 (56%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
+YD + F+ V++ ++ +EDFG ANA R+L YR+K CTFNDDIQ
Sbjct: 214 EYDAFIASFVN-VMKEVFPKAILHWEDFGRANASRILHNYRDKICTFNDDIQGTGAMVVA 272
Query: 446 XXXXSLRLTQKRLSDNVIV 502
++++++ LS+ I+
Sbjct: 273 AVLATIQVSRIPLSEQKII 291
>UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 549
Score = 84.6 bits (200), Expect = 2e-15
Identities = 37/80 (46%), Positives = 52/80 (65%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
+L NWP + IVVTDG R+LGLGDLG GM I VGK++LY A GG P + +P+ +D+
Sbjct: 130 MLDNWPSPETEIIVVTDGGRVLGLGDLGTNGMAISVGKVSLYVASGGFDPAKSMPVCLDL 189
Query: 195 GTNTQSMLDDPLYIGLRQRR 254
GT+ ++ Y+G + R
Sbjct: 190 GTSNVALRAHDFYLGADEPR 209
Score = 53.2 bits (122), Expect = 7e-06
Identities = 31/84 (36%), Positives = 43/84 (51%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G + ++ EF AV ++ NCL+QFEDF +AFR+L+ R+K FNDDIQ
Sbjct: 210 LTGDAHLAVVTEFCLAVKDKW-PNCLIQFEDFKTEDAFRILDHMRHKVLCFNDDIQGTGA 268
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
+LR D I+F
Sbjct: 269 TILAGLINALRAQGTPTKDARIIF 292
>UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 633
Score = 84.6 bits (200), Expect = 2e-15
Identities = 38/84 (45%), Positives = 52/84 (61%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
HV +L NWP VR ++V++G +L +GDLG M I L GGI P CL +
Sbjct: 189 HVSQILSNWPFRCVRCLLVSNGASVLSVGDLGVDEMPILFSNLHQNVVYGGIHPAYCLAV 248
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+DVGTN + +L+DP+Y GLR+RR
Sbjct: 249 MLDVGTNNEELLNDPMYTGLRERR 272
Score = 47.2 bits (107), Expect = 4e-04
Identities = 26/83 (31%), Positives = 39/83 (46%)
Frame = +2
Query: 269 YDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXXX 448
YD+L +EF +V+++YG L+ +DF A + LE YR + C + D Q
Sbjct: 278 YDQLFEEFTLSVMQQYGPRALILCKDFEAQKAKKQLELYRERQCIVDVDFQCFAAVALAG 337
Query: 449 XXXSLRLTQKRLSDNVIVFQEPE 517
RL + S NV +F E
Sbjct: 338 VIVCNRLKRVFFSSNVFLFYGAE 360
Score = 38.3 bits (85), Expect = 0.20
Identities = 21/49 (42%), Positives = 31/49 (63%), Gaps = 4/49 (8%)
Frame = +1
Query: 544 CVMAMKGEGTPEQEARCRIWMVDSKGLIV---KNRPEEDLTF-TRRDSL 678
C++ +K EG E +AR +IW D+ GL+V K+ PEE L F +RD +
Sbjct: 370 CMVLLKREGLIEMKAREKIWFFDANGLVVLGRKDIPEELLEFANQRDPI 418
>UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia
intestinalis|Rep: Malic enzyme - Giardia lamblia
(Giardia intestinalis)
Length = 557
Score = 83.4 bits (197), Expect = 5e-15
Identities = 41/83 (49%), Positives = 51/83 (61%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
V + WP + R IV TDG RILGLGDLG G I VGKL LY+ GG P LPI+
Sbjct: 124 VKQIFDQWPYSP-RIIVATDGTRILGLGDLGTGGHQICVGKLTLYSLGGGFAPEHTLPIS 182
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
D G NT + +DP Y+G+ ++R
Sbjct: 183 FDFGCNTDKIREDPHYLGIPEKR 205
Score = 46.4 bits (105), Expect = 8e-04
Identities = 23/51 (45%), Positives = 33/51 (64%)
Frame = +2
Query: 269 YDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
Y ++ E + AV ++ +C+ QFEDF N AF LL+K+R+ FNDDIQ
Sbjct: 211 YYGIIKETIEAVHSKW-PDCIFQFEDFSNDTAFTLLKKHRDAGPVFNDDIQ 260
>UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10;
Francisella tularensis|Rep: NAD-dependent malic enzyme -
Francisella tularensis subsp. novicida (strain U112)
Length = 604
Score = 82.2 bits (194), Expect = 1e-14
Identities = 34/84 (40%), Positives = 55/84 (65%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPI 182
H+ +L+ + + ++VTDGE +LG+GD G GM I +GK+ +Y A GI P + LP+
Sbjct: 144 HIARILERYEYNSIDLVLVTDGEAVLGIGDQGIGGMNISIGKIMVYVAASGIDPARVLPV 203
Query: 183 TIDVGTNTQSMLDDPLYIGLRQRR 254
+D+GTN ++L+ P Y+G+R R
Sbjct: 204 QLDMGTNNDALLNAPGYLGVRLPR 227
Score = 61.3 bits (142), Expect = 3e-08
Identities = 27/54 (50%), Positives = 37/54 (68%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
G YDE ++EF+ V R+ N + +ED G NA R+LEKY++K CTFNDDI+
Sbjct: 230 GETYDEFIEEFVTQVKARF-PNVFLHWEDLGRDNATRILEKYKDKLCTFNDDIE 282
>UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium
globosum|Rep: Malic enzyme - Chaetomium globosum (Soil
fungus)
Length = 586
Score = 78.6 bits (185), Expect = 2e-13
Identities = 34/64 (53%), Positives = 48/64 (75%)
Frame = +3
Query: 63 DGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLDDPLYIGL 242
DGE ILG+GD G G+ I V KLAL T GI P++ LP+ +D GT+ +++L+DPLY+GL
Sbjct: 182 DGEEILGIGDQGCGGILISVAKLALMTLCAGIHPNRVLPVVLDCGTDNETLLNDPLYLGL 241
Query: 243 RQRR 254
R++R
Sbjct: 242 REKR 245
>UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep:
Malolactic enzyme - Oenococcus oeni (Leuconostoc oenos)
Length = 541
Score = 78.2 bits (184), Expect = 2e-13
Identities = 37/85 (43%), Positives = 52/85 (61%), Gaps = 1/85 (1%)
Frame = +3
Query: 3 HVYDVLKNWPE-TDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLP 179
++ LKN D++ +VV+D E ILG+GD G G+ I VGKL +YT GI P L
Sbjct: 122 NIQSTLKNAANGRDIKLLVVSDAEGILGIGDWGVQGVDIAVGKLMVYTVAAGIDPSTVLA 181
Query: 180 ITIDVGTNTQSMLDDPLYIGLRQRR 254
+ ID GTN + +L DP+Y+G + R
Sbjct: 182 VVIDAGTNNEKLLKDPMYLGNKFNR 206
Score = 53.2 bits (122), Expect = 7e-06
Identities = 25/79 (31%), Positives = 44/79 (55%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RG Y + +D+F+ + N + +EDFG +NA +L Y++K TFNDDIQ
Sbjct: 207 VRGDKYYDFIDKFVNHAESLF-PNLYLHWEDFGRSNASNILNSYKDKIATFNDDIQGTGI 265
Query: 434 XXXXXXXXSLRLTQKRLSD 490
+L+++ ++L+D
Sbjct: 266 VVLAGVLGALKISGQKLTD 284
>UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|Rep:
Malic enzyme - Aspergillus niger
Length = 609
Score = 77.4 bits (182), Expect = 4e-13
Identities = 38/72 (52%), Positives = 49/72 (68%)
Frame = +3
Query: 39 DVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSML 218
+V IVV+DGE ILG+GD G + I V KLAL T GI P + LP+ +D GT+ +S+L
Sbjct: 187 EVDYIVVSDGEEILGIGDQGVGAILISVAKLALTTLCAGIHPSRQLPVVLDCGTDNESLL 246
Query: 219 DDPLYIGLRQRR 254
D LY+GLRQ R
Sbjct: 247 TDELYLGLRQSR 258
Score = 52.8 bits (121), Expect = 9e-06
Identities = 22/56 (39%), Positives = 39/56 (69%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
+RG +YD+ +++F+ +R+ + + FEDFG NA R+L+K++++ FNDDIQ
Sbjct: 259 VRGEEYDQFVEKFVETARKRFPR-AYIHFEDFGLHNAKRILDKFKSRIPCFNDDIQ 313
>UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 629
Score = 77.0 bits (181), Expect = 5e-13
Identities = 34/77 (44%), Positives = 49/77 (63%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
N P+ + +VVTD E ILG+GD G G+ I K ALYT GI P++ LP+ +D GT+
Sbjct: 192 NKPDDAIDLVVVTDAEAILGIGDQGVGGITISTSKAALYTLGAGINPNRILPVVLDCGTD 251
Query: 204 TQSMLDDPLYIGLRQRR 254
++ DPLY+G ++ R
Sbjct: 252 NHALFSDPLYMGWKRTR 268
Score = 65.3 bits (152), Expect = 2e-09
Identities = 34/83 (40%), Positives = 48/83 (57%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RG +YD+ +D F+ R N ++ FEDFG ANA+RL+EKY+N FNDDIQ
Sbjct: 269 IRGKNYDQFIDRFITNC-RELFPNAIIHFEDFGMANAYRLMEKYKN-IPMFNDDIQGTGA 326
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
++++ RLSD IV
Sbjct: 327 VALAALLSAIKVAGSRLSDQRIV 349
>UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=15; Saccharomycetales|Rep: NAD-dependent
malic enzyme, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 669
Score = 73.3 bits (172), Expect = 6e-12
Identities = 35/69 (50%), Positives = 46/69 (66%)
Frame = +3
Query: 33 ETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQS 212
+ DV IVV+D E ILG+GD G G+ I + KLAL T GGI P + LP+ +DVGTN +
Sbjct: 228 DKDVDYIVVSDSEGILGIGDQGIGGVRIAISKLALMTLCGGIHPGRVLPVCLDVGTNNKK 287
Query: 213 MLDDPLYIG 239
+ D LY+G
Sbjct: 288 LARDELYMG 296
Score = 64.5 bits (150), Expect = 3e-09
Identities = 33/83 (39%), Positives = 50/83 (60%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RG YD+ L++F++AV + Y + ++ FEDFG NA RLLEKYR + +FNDDIQ
Sbjct: 302 IRGKQYDDFLEKFIKAVKKVY-PSAVLHFEDFGVKNARRLLEKYRYELPSFNDDIQGTGA 360
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
+L+ T + L D ++
Sbjct: 361 VVMASLIAALKHTNRDLKDTRVL 383
>UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococcus
sp. WH 5701|Rep: Malate oxidoreductase - Synechococcus
sp. WH 5701
Length = 517
Score = 71.7 bits (168), Expect = 2e-11
Identities = 35/83 (42%), Positives = 51/83 (61%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ VL V I++TD + ILG+GD G G+ I +GKLA+YT G+ P + LP+
Sbjct: 87 IESVLSQAATGPVDLILITDSQGILGIGDQGIGGIEICLGKLAVYTLCAGLDPARVLPLV 146
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
+DVGT+ +L++PLY G R R
Sbjct: 147 LDVGTDRVELLENPLYPGWRHPR 169
Score = 50.0 bits (114), Expect = 6e-05
Identities = 25/56 (44%), Positives = 36/56 (64%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
L G DYD L + A+ R++ L+ +EDFG +A +LLE+YR + +FNDDIQ
Sbjct: 170 LDGLDYDRFLARSVEALQRQF-PGALLHWEDFGTGHAHQLLERYRLQLPSFNDDIQ 224
>UniRef50_Q5C370 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 264
Score = 71.3 bits (167), Expect = 2e-11
Identities = 32/82 (39%), Positives = 47/82 (57%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y +DE + ++ YG N + F D N+NAF+LLE+YR Y TFNDDIQ
Sbjct: 3 GEEYISFMDEVVESLSAHYGPNVCLHFADLKNSNAFQLLERYRPNYITFNDDIQGSAVVI 62
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+ R+T+K++S N +F
Sbjct: 63 VSGLLTASRITKKKISQNTYLF 84
Score = 37.9 bits (84), Expect = 0.27
Identities = 21/49 (42%), Positives = 31/49 (63%)
Frame = +1
Query: 547 VMAMKGEGTPEQEARCRIWMVDSKGLIVKNRPEEDLTFTRRDSLRTTRP 693
V A+ EG E EA+ RI+M+DS+GL+V NR +LT + + R+ P
Sbjct: 99 VQAIIEEGFTEDEAKSRIFMMDSRGLLVTNR---ELTKAKSEFARSDYP 144
>UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 216
Score = 70.5 bits (165), Expect = 4e-11
Identities = 30/41 (73%), Positives = 35/41 (85%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVG 125
H+ ++L NWPE V+AIV TDGERILGLGDLGA GMGIP+G
Sbjct: 171 HIPEILNNWPEPIVKAIVFTDGERILGLGDLGAYGMGIPIG 211
>UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3;
Schizosaccharomyces pombe|Rep: NAD-dependent malic
enzyme - Schizosaccharomyces pombe (Fission yeast)
Length = 565
Score = 64.9 bits (151), Expect = 2e-09
Identities = 34/85 (40%), Positives = 52/85 (61%), Gaps = 1/85 (1%)
Frame = +3
Query: 3 HVYDVLKNWPETD-VRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLP 179
++ L + ++D V I++TD E ILG+GD G G+ I V K L T G+ P++ LP
Sbjct: 135 YIKQQLSEFGKSDSVEYIIITDSEGILGIGDQGVGGVLISVAKGHLMTLCAGLDPNRFLP 194
Query: 180 ITIDVGTNTQSMLDDPLYIGLRQRR 254
I +DVGTN ++ + Y+GLR+ R
Sbjct: 195 IVLDVGTNNETHRKNHQYMGLRKDR 219
Score = 56.8 bits (131), Expect = 5e-07
Identities = 30/83 (36%), Positives = 43/83 (51%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
+RG YD LD ++A+ R + FEDFG ANA R+L+ YR FNDDIQ
Sbjct: 220 VRGEQYDSFLDNVIKAI-REVFPEAFIHFEDFGLANAKRILDHYRPDIACFNDDIQGTGA 278
Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
+L +T+ L++ I+
Sbjct: 279 VALAAIIGALHVTKSPLTEQRIM 301
>UniRef50_Q27113 Cluster: Malic enzyme; n=1; Trichomonas
vaginalis|Rep: Malic enzyme - Trichomonas vaginalis
Length = 434
Score = 61.3 bits (142), Expect = 3e-08
Identities = 33/96 (34%), Positives = 50/96 (52%)
Frame = +2
Query: 134 ALHRARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGPDYDELLDEFMRAVVRR 313
+LH R S ++ R GY + RP + R + ++ + + EF+ AV
Sbjct: 61 SLHTYRPSXPRSDTSSPVRYGYRQKGNPRRPTLPRLAPSTCTCAEHLKFVTEFVEAVKEV 120
Query: 314 YGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
+G CLVQFEDF AF+LL+ +R + FNDDI+
Sbjct: 121 FGDTCLVQFEDFEMETAFKLLDHFRWRCNCFNDDIE 156
>UniRef50_A7R628 Cluster: Chromosome undetermined scaffold_1141,
whole genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome undetermined scaffold_1141, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 236
Score = 52.4 bits (120), Expect = 1e-05
Identities = 28/80 (35%), Positives = 42/80 (52%)
Frame = +2
Query: 266 DYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXXXX 445
+Y E L EFM AV + YG+ + +FED+ N NAF LL +Y + N DIQ
Sbjct: 129 EYSEFLHEFMSAVNQNYGEK-VTRFEDYANHNAFELLARYNTTHLVPNGDIQGTTYVVLS 187
Query: 446 XXXXSLRLTQKRLSDNVIVF 505
+L+L L+++ +F
Sbjct: 188 GLVAALKLFGGTLANHKFLF 207
>UniRef50_A5B3Q7 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 176
Score = 52.4 bits (120), Expect = 1e-05
Identities = 29/84 (34%), Positives = 44/84 (52%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G +Y E L +FM V + YG+ + +FED+ N NAF LL +Y + N DIQ
Sbjct: 76 LPGKEYSEFLHKFMSTVKQNYGEK-VTRFEDYANHNAFELLARYNTTHLVPNGDIQXTTY 134
Query: 434 XXXXXXXXSLRLTQKRLSDNVIVF 505
+L+L L+++ +F
Sbjct: 135 VVLSGLVAALKLFGGTLANHKFLF 158
>UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n=1;
Zea mays|Rep: Putative NADP-dependent malic enzyme - Zea
mays (Maize)
Length = 309
Score = 51.6 bits (118), Expect = 2e-05
Identities = 22/31 (70%), Positives = 25/31 (80%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLG 98
V DVLKNWP +++ I VTD ERILGLGDLG
Sbjct: 251 VLDVLKNWPHRNIQVIFVTDSERILGLGDLG 281
>UniRef50_Q3W7J3 Cluster: Malate dehydrogenase; n=1; Frankia sp.
EAN1pec|Rep: Malate dehydrogenase - Frankia sp. EAN1pec
Length = 464
Score = 43.2 bits (97), Expect = 0.007
Identities = 24/58 (41%), Positives = 36/58 (62%), Gaps = 1/58 (1%)
Frame = +3
Query: 51 IVVTDGERILGLGDLG-ACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLD 221
IVVTDG +LGLGD+G A + + GK AL+ GG+ +PI +D T+ + ++D
Sbjct: 142 IVVTDGTAVLGLGDIGPAAALPVMEGKAALFKHFGGV---DAVPICLDC-TDVEEIVD 195
>UniRef50_A4EC16 Cluster: Putative uncharacterized protein; n=1;
Collinsella aerofaciens ATCC 25986|Rep: Putative
uncharacterized protein - Collinsella aerofaciens ATCC
25986
Length = 298
Score = 41.9 bits (94), Expect = 0.017
Identities = 33/76 (43%), Positives = 38/76 (50%), Gaps = 4/76 (5%)
Frame = +2
Query: 113 HPCGQTRA-LHRARRHQASSVSTHHYR---RGYEHPVDAGRPAVHRAPAAALRGPDYDEL 280
+PC TRA LH A AS T YR RG A R A+ RAPA LR P D+L
Sbjct: 82 YPCFCTRAELHAASAPHASD-GTPIYRGACRGLSAEEVARRSAL-RAPATRLRVPAVDDL 139
Query: 281 LDEFMRAVVRRYGQNC 328
D+ + V R YG C
Sbjct: 140 ADDVVEFVDRTYGAQC 155
>UniRef50_Q8U225 Cluster: Malate oxidoreductase; n=41; cellular
organisms|Rep: Malate oxidoreductase - Pyrococcus
furiosus
Length = 435
Score = 41.5 bits (93), Expect = 0.022
Identities = 24/47 (51%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = +3
Query: 54 VVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 191
VVTDG ILGLG++G GM + GK L+ AL GI PI ID
Sbjct: 77 VVTDGSAILGLGNIGVLAGMPVMEGKCVLFKALAGI---DAFPILID 120
>UniRef50_Q5Y0M3 Cluster: Malate oxidoreductase; n=8; cellular
organisms|Rep: Malate oxidoreductase - uncultured
archaeon GZfos12E1
Length = 444
Score = 39.9 bits (89), Expect = 0.067
Identities = 20/47 (42%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +3
Query: 54 VVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 191
+VTDG R+LGLGD+GA + + GK L+ LGG+ P+ +D
Sbjct: 84 IVTDGTRVLGLGDVGAEAALPVMEGKAMLFKYLGGV---DAFPLCLD 127
>UniRef50_P54572 Cluster: Probable NAD-dependent malic enzyme 1;
n=4; Bacteria|Rep: Probable NAD-dependent malic enzyme 1
- Bacillus subtilis
Length = 439
Score = 39.9 bits (89), Expect = 0.067
Identities = 22/47 (46%), Positives = 30/47 (63%), Gaps = 1/47 (2%)
Frame = +3
Query: 54 VVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 191
+VTDG ILGLG++G+ GM + GK AL+ L GI +PI +D
Sbjct: 142 IVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISG---IPILLD 185
>UniRef50_Q2KKD0 Cluster: Oxalacetate decarboxylase; n=11;
Lactobacillales|Rep: Oxalacetate decarboxylase -
Enterococcus faecalis (Streptococcus faecalis)
Length = 390
Score = 39.1 bits (87), Expect = 0.12
Identities = 18/51 (35%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = +3
Query: 9 YDVLKNWPETDVRAIVVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGI 158
+++ + W + V+TDG +LGLG++G G+ I GK LY L G+
Sbjct: 49 HELAREWTISGKLIAVITDGSAVLGLGNMGTQAGLPIVEGKALLYKNLAGV 99
>UniRef50_A0DE35 Cluster: Malic enzyme; n=3; Paramecium
tetraurelia|Rep: Malic enzyme - Paramecium tetraurelia
Length = 687
Score = 39.1 bits (87), Expect = 0.12
Identities = 18/38 (47%), Positives = 27/38 (71%), Gaps = 1/38 (2%)
Frame = +3
Query: 48 AIVVTDGERILGLGDLG-ACGMGIPVGKLALYTALGGI 158
A ++T+G ILGLG++G + G+ + GK L+ ALGGI
Sbjct: 337 AAIITNGTAILGLGNIGPSAGLPVMEGKSVLFNALGGI 374
>UniRef50_Q8VW43 Cluster: Proline dehydrogenase; n=11;
Proteobacteria|Rep: Proline dehydrogenase -
Bradyrhizobium japonicum
Length = 1017
Score = 37.1 bits (82), Expect = 0.47
Identities = 21/60 (35%), Positives = 28/60 (46%)
Frame = +2
Query: 86 GRFGRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGP 265
GR R HP + + HR R H+ + +RR H +D GRP H+ AA GP
Sbjct: 692 GRSPAARSRHPQERAASRHRRRPHRRRADRASRHRRRRLHRLDRGRPQ-HQRTLAAKDGP 750
>UniRef50_Q9HKY7 Cluster: Malate oxidoreductase (Malic enzyme)
related protein; n=17; Archaea|Rep: Malate
oxidoreductase (Malic enzyme) related protein -
Thermoplasma acidophilum
Length = 467
Score = 36.7 bits (81), Expect = 0.62
Identities = 19/48 (39%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +3
Query: 54 VVTDGERILGLGDLG-ACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
++TDG R+LGLG++G M + GK ++ LGG+ +PI I V
Sbjct: 94 ILTDGTRVLGLGNIGPEAAMPVMEGKALIFNYLGGV---NAIPIPIRV 138
>UniRef50_Q73UK5 Cluster: Putative uncharacterized protein; n=1;
Mycobacterium avium subsp. paratuberculosis|Rep:
Putative uncharacterized protein - Mycobacterium
paratuberculosis
Length = 291
Score = 36.3 bits (80), Expect = 0.82
Identities = 25/62 (40%), Positives = 30/62 (48%), Gaps = 3/62 (4%)
Frame = +2
Query: 32 GDGRPRHCCNRRRTYFGPGRFGRV---RDGHPCGQTRALHRARRHQASSVSTHHYRRGYE 202
G GRPR R+ PGR +V R G P G A HR RR + HH +RG+
Sbjct: 143 GPGRPR----RQHLRVVPGRLPQVLRRRGGRPAGDLAA-HR-RRQARPVAADHHRQRGHH 196
Query: 203 HP 208
HP
Sbjct: 197 HP 198
>UniRef50_A1B0A4 Cluster: Beta-ketoacyl synthase; n=1; Paracoccus
denitrificans PD1222|Rep: Beta-ketoacyl synthase -
Paracoccus denitrificans (strain Pd 1222)
Length = 2093
Score = 35.9 bits (79), Expect = 1.1
Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 3/41 (7%)
Frame = +1
Query: 595 RIWMVDSKGLIVKNRPEEDLTFTRRD---SLRTTRPSGPWL 708
R W + ++GL+ ++P EDL RD LR +P GP+L
Sbjct: 1850 RFWGIQARGLLGDDKPHEDLAEAARDYIEELRQVQPEGPYL 1890
>UniRef50_Q2UES6 Cluster: Predicted protein; n=6;
Trichocomaceae|Rep: Predicted protein - Aspergillus
oryzae
Length = 139
Score = 35.5 bits (78), Expect = 1.4
Identities = 28/70 (40%), Positives = 34/70 (48%), Gaps = 5/70 (7%)
Frame = +2
Query: 128 TRALHRARRHQASSVSTHHYRRGYEHPVDAGRPAV-----HRAPAAALRGPDYDELLDEF 292
TRA+ RAR S+S H RRG PV P V H P +R D DE + EF
Sbjct: 37 TRAVSRAR-----SISVHGRRRGRSSPVRMVEPYVESSSSHAGPLIVVRPRDSDEDIREF 91
Query: 293 MRAVVRRYGQ 322
M + RR G+
Sbjct: 92 M-PLARRSGE 100
>UniRef50_Q8R7R6 Cluster: Malic enzyme; n=10; cellular
organisms|Rep: Malic enzyme - Thermoanaerobacter
tengcongensis
Length = 394
Score = 34.7 bits (76), Expect = 2.5
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +3
Query: 54 VVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLPITIDVGTNTQSMLD 221
VVTDG +LGLG++G + + GK L+ GGI T DV +++L+
Sbjct: 66 VVTDGSAVLGLGNIGPYAALPVMEGKAVLFKEFGGIDAIPICLATQDVDEIIRTVLN 122
>UniRef50_Q0STR8 Cluster: Malate oxidoreductase; n=2; Clostridium
perfringens|Rep: Malate oxidoreductase - Clostridium
perfringens (strain SM101 / Type A)
Length = 381
Score = 34.7 bits (76), Expect = 2.5
Identities = 22/69 (31%), Positives = 33/69 (47%), Gaps = 2/69 (2%)
Frame = +3
Query: 3 HVYDVLKNWPETDVRAIVVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLP 179
H +LK V+TDG +LGLG++G G I K +Y L G+ +P
Sbjct: 48 HHPSMLKTLTSVGNSIAVITDGTAVLGLGNIGTLAGYPIVEAKALVYKDLAGV---NAIP 104
Query: 180 ITID-VGTN 203
+ +D +G N
Sbjct: 105 LCVDQIGCN 113
>UniRef50_Q2STW7 Cluster: Putative uncharacterized protein; n=1;
Burkholderia thailandensis E264|Rep: Putative
uncharacterized protein - Burkholderia thailandensis
(strain E264 / ATCC 700388 / DSM 13276 /CIP 106301)
Length = 647
Score = 34.3 bits (75), Expect = 3.3
Identities = 17/43 (39%), Positives = 22/43 (51%)
Frame = +3
Query: 12 DVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALY 140
DVL+ +P T V DG + GLG+L CG P +L Y
Sbjct: 97 DVLRQFPHTYVERSPSGDGLHLFGLGELVECGKAGPGNRLECY 139
>UniRef50_UPI00015555BF Cluster: PREDICTED: similar to
leishmanolysin-like (metallopeptidase M8 family),
partial; n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
similar to leishmanolysin-like (metallopeptidase M8
family), partial - Ornithorhynchus anatinus
Length = 639
Score = 33.9 bits (74), Expect = 4.4
Identities = 13/45 (28%), Positives = 22/45 (48%)
Frame = +1
Query: 118 LWANSRSTPRSEASSLISVYPSLSTWVRTPSRCWTTRCTSGSGSG 252
LW+++ P ++ ++ +P W T SRCW C + SG
Sbjct: 171 LWSSTYDLPAPREAAWLAWWPPSGFWWPTSSRCWPASCCACGTSG 215
>UniRef50_Q61829 Cluster: MOD-1 null malic enzyme; n=1; Mus
musculus|Rep: MOD-1 null malic enzyme - Mus musculus
(Mouse)
Length = 332
Score = 33.9 bits (74), Expect = 4.4
Identities = 15/36 (41%), Positives = 22/36 (61%)
Frame = +3
Query: 651 LNVHKERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
L KE FA +H ++ L +V+ +P+ LIG AAI
Sbjct: 105 LTEEKEVFAHEHEEMKNLEAIVQKIKPTALIGVAAI 140
>UniRef50_Q01PP2 Cluster: Heparinase II/III family protein; n=1;
Solibacter usitatus Ellin6076|Rep: Heparinase II/III
family protein - Solibacter usitatus (strain Ellin6076)
Length = 641
Score = 33.9 bits (74), Expect = 4.4
Identities = 17/59 (28%), Positives = 28/59 (47%)
Frame = -1
Query: 704 QGPDGRVVLSESLLVNVKSSSGLFLTMRPFESTIQMRHLASCSGVPSPFIAITHNSAIP 528
+GP G L + ++ + +G F P + R LA CS P+P + + SA+P
Sbjct: 541 EGPAGEHTLEQFWHLSALADAGRFSFSAPARTVESWRSLALCSRQPAPVLTVELRSALP 599
>UniRef50_UPI0000DD86B8 Cluster: PREDICTED: hypothetical protein;
n=1; Homo sapiens|Rep: PREDICTED: hypothetical protein -
Homo sapiens
Length = 646
Score = 33.5 bits (73), Expect = 5.8
Identities = 20/58 (34%), Positives = 24/58 (41%)
Frame = +2
Query: 98 RVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGPDY 271
RV HP + + AR Q + ST R AGRP V R P LR P +
Sbjct: 111 RVASAHPAERLQPRASARARQLAVQSTRPSRPPGFRVSRAGRPGVQRGPGLRLRAPSW 168
>UniRef50_UPI0000DD8673 Cluster: PREDICTED: hypothetical protein;
n=1; Homo sapiens|Rep: PREDICTED: hypothetical protein -
Homo sapiens
Length = 694
Score = 33.5 bits (73), Expect = 5.8
Identities = 20/58 (34%), Positives = 24/58 (41%)
Frame = +2
Query: 98 RVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGPDY 271
RV HP + + AR Q + ST R AGRP V R P LR P +
Sbjct: 60 RVASAHPAERLQPRASARARQLAVQSTRPSRPPGFRVSRAGRPGVQRGPGLRLRAPSW 117
>UniRef50_UPI0000D9B78D Cluster: PREDICTED: hypothetical protein;
n=2; Catarrhini|Rep: PREDICTED: hypothetical protein -
Macaca mulatta
Length = 686
Score = 33.5 bits (73), Expect = 5.8
Identities = 24/71 (33%), Positives = 28/71 (39%)
Frame = +2
Query: 53 CCNRRRTYFGPGRFGRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHPVDAGRPAV 232
CC RRR GP F + R H + AL ++ S R P
Sbjct: 170 CCRRRRRLPGPRSFPQRRQ-HLPAEPIALINIQQQAKGEGSERDLPRPIPPPPPPPHTPT 228
Query: 233 HRAPAAALRGP 265
RAPAAAL GP
Sbjct: 229 ARAPAAALSGP 239
>UniRef50_UPI00006CF21C Cluster: Bowman-Birk serine protease
inhibitor family protein; n=1; Tetrahymena thermophila
SB210|Rep: Bowman-Birk serine protease inhibitor family
protein - Tetrahymena thermophila SB210
Length = 1467
Score = 33.1 bits (72), Expect = 7.7
Identities = 20/59 (33%), Positives = 26/59 (44%)
Frame = +3
Query: 18 LKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
L NW IVV+ G + L LG G +P + + G+ PH L TIDV
Sbjct: 208 LVNWTCLQKNVIVVSGGIQNWALTSLGPLGQNVPASR-----TISGLTPHNQLIFTIDV 261
>UniRef50_Q6A639 Cluster: Conserved protein; n=3;
Actinomycetales|Rep: Conserved protein -
Propionibacterium acnes
Length = 506
Score = 33.1 bits (72), Expect = 7.7
Identities = 20/56 (35%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Frame = -2
Query: 409 VESAVLVPILLE-EPKSVSVPEVLELHETVLPVAAHDGAHELVEQLVVVGAAQRRC 245
+++ V VPILL E S + L++ V VAAHDG ++ + A Q RC
Sbjct: 203 IKAMVTVPILLPGEKTSTRIEPAASLYDMVPEVAAHDGVTDVGVWIGFAWADQPRC 258
>UniRef50_Q9FB23 Cluster: Peptide synthetase NRPS5-4-3; n=1;
Streptomyces verticillus|Rep: Peptide synthetase
NRPS5-4-3 - Streptomyces verticillus
Length = 2675
Score = 33.1 bits (72), Expect = 7.7
Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +2
Query: 176 THHYRR-GYEHPVDAGRPAVHRAPAAALRGPDYDELLDEFMRAVVRRY 316
TH R YE + A H A ALRGP +E L E +R VVRR+
Sbjct: 668 THGQRALWYEQALAPHAAAYHLVRALALRGPVDEEALAEAVRRVVRRH 715
>UniRef50_Q2CHG8 Cluster: Type I secretion target repeat protein; n=1;
Oceanicola granulosus HTCC2516|Rep: Type I secretion
target repeat protein - Oceanicola granulosus HTCC2516
Length = 11286
Score = 33.1 bits (72), Expect = 7.7
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = +2
Query: 140 HRARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGPDYDELLD 286
H H HH G+ + A RPA APA+ LR + +LD
Sbjct: 11045 HHGHGHHGHGHHGHHGHHGHHGHLVASRPAAGAAPASTLRAAEASFMLD 11093
>UniRef50_A0UI99 Cluster: Putative uncharacterized protein; n=1;
Burkholderia multivorans ATCC 17616|Rep: Putative
uncharacterized protein - Burkholderia multivorans ATCC
17616
Length = 530
Score = 33.1 bits (72), Expect = 7.7
Identities = 19/46 (41%), Positives = 23/46 (50%)
Frame = +2
Query: 143 RARRHQASSVSTHHYRRGYEHPVDAGRPAVHRAPAAALRGPDYDEL 280
RA RH VS +R E+ V A+ R P +ALR PD EL
Sbjct: 54 RAARHAVRHVSPPRFRSRRENRVRPADVALPRRPRSALRDPDAGEL 99
>UniRef50_Q7XBC7 Cluster: Putative transposase protein; n=2; Zea
mays|Rep: Putative transposase protein - Zea mays
(Maize)
Length = 757
Score = 33.1 bits (72), Expect = 7.7
Identities = 22/73 (30%), Positives = 34/73 (46%), Gaps = 3/73 (4%)
Frame = +2
Query: 110 GHPCGQTRALHRARRHQASSVSTHHYRRG---YEHPVDAGRPAVHRAPAAALRGPDYDEL 280
G PC A A + +A+S T++ G Y +P R + + A A + GPDYD
Sbjct: 622 GAPCSTFSAYAMAHKGKATSDVTYNPDDGPEAYTNPAIHSRLSEYTAMAKEVHGPDYDPR 681
Query: 281 LDEFMRAVVRRYG 319
++ V+ R G
Sbjct: 682 TEDIDGDVLMRVG 694
>UniRef50_A0DQ15 Cluster: Chromosome undetermined scaffold_6, whole
genome shotgun sequence; n=3; Paramecium
tetraurelia|Rep: Chromosome undetermined scaffold_6,
whole genome shotgun sequence - Paramecium tetraurelia
Length = 238
Score = 33.1 bits (72), Expect = 7.7
Identities = 13/29 (44%), Positives = 21/29 (72%)
Frame = +1
Query: 577 EQEARCRIWMVDSKGLIVKNRPEEDLTFT 663
E+ CR++++ + LI+KN+ EE LTFT
Sbjct: 7 EEMIPCRLYLLKTDKLIIKNKQEEGLTFT 35
>UniRef50_A6RMY7 Cluster: Predicted protein; n=1; Botryotinia
fuckeliana B05.10|Rep: Predicted protein - Botryotinia
fuckeliana B05.10
Length = 226
Score = 33.1 bits (72), Expect = 7.7
Identities = 20/55 (36%), Positives = 31/55 (56%), Gaps = 3/55 (5%)
Frame = -1
Query: 683 VLSESLLVNVKSSSGLFLTMRPF--ESTIQMRHLA-SCSGVPSPFIAITHNSAIP 528
+LS LL+++ + LFLT+RP ES + +RHL S P +++ T IP
Sbjct: 87 LLSPLLLISISIALLLFLTLRPHTSESLLILRHLGLQISSSPKTYLSTTKTRFIP 141
>UniRef50_Q92797 Cluster: Symplekin; n=89; Eukaryota|Rep: Symplekin -
Homo sapiens (Human)
Length = 1274
Score = 33.1 bits (72), Expect = 7.7
Identities = 16/32 (50%), Positives = 19/32 (59%)
Frame = +3
Query: 600 LDGGLEGPHRQEQTGGGLNVHKERFAQDHAPV 695
LD LEGP +E GGL + +ER Q APV
Sbjct: 1219 LDSSLEGPLPKETAAGGLTLKEERSPQTLAPV 1250
>UniRef50_P16468 Cluster: NAD-dependent malic enzyme; n=32;
Bacteria|Rep: NAD-dependent malic enzyme - Bacillus
stearothermophilus (Geobacillus stearothermophilus)
Length = 478
Score = 33.1 bits (72), Expect = 7.7
Identities = 18/47 (38%), Positives = 25/47 (53%), Gaps = 1/47 (2%)
Frame = +3
Query: 54 VVTDGERILGLGDLGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 191
VV+DG +LGLGD+G M + GK L+ G+ PI +D
Sbjct: 144 VVSDGTAVLGLGDIGPYAAMPVMEGKAMLFKEFAGV---DAFPICLD 187
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 705,887,111
Number of Sequences: 1657284
Number of extensions: 14039820
Number of successful extensions: 51074
Number of sequences better than 10.0: 95
Number of HSP's better than 10.0 without gapping: 48413
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 51031
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 62969581935
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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