Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00271
         (758 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|...    65   1e-11
SPAC10F6.15 |||S. pombe specific UPF0300 family protein 1|Schizo...    28   1.7  
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa...    28   1.7  
SPAC25B8.13c |isp7||2-OG-Fe|Schizosaccharomyces pombe|chr 1|||Ma...    27   3.8  
SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c...    26   6.7  
SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subuni...    25   8.9  

>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 565

 Score = 64.9 bits (151), Expect = 1e-11
 Identities = 34/85 (40%), Positives = 52/85 (61%), Gaps = 1/85 (1%)
 Frame = +3

Query: 3   HVYDVLKNWPETD-VRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLP 179
           ++   L  + ++D V  I++TD E ILG+GD G  G+ I V K  L T   G+ P++ LP
Sbjct: 135 YIKQQLSEFGKSDSVEYIIITDSEGILGIGDQGVGGVLISVAKGHLMTLCAGLDPNRFLP 194

Query: 180 ITIDVGTNTQSMLDDPLYIGLRQRR 254
           I +DVGTN ++   +  Y+GLR+ R
Sbjct: 195 IVLDVGTNNETHRKNHQYMGLRKDR 219



 Score = 56.8 bits (131), Expect = 3e-09
 Identities = 30/83 (36%), Positives = 43/83 (51%)
 Frame = +2

Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
           +RG  YD  LD  ++A+ R       + FEDFG ANA R+L+ YR     FNDDIQ    
Sbjct: 220 VRGEQYDSFLDNVIKAI-REVFPEAFIHFEDFGLANAKRILDHYRPDIACFNDDIQGTGA 278

Query: 434 XXXXXXXXSLRLTQKRLSDNVIV 502
                   +L +T+  L++  I+
Sbjct: 279 VALAAIIGALHVTKSPLTEQRIM 301


>SPAC10F6.15 |||S. pombe specific UPF0300 family protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 432

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 15/43 (34%), Positives = 18/43 (41%)
 Frame = +2

Query: 83  PGRFGRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYEHPV 211
           P +   + D H C QT   H  R HQA       Y R  E P+
Sbjct: 128 PDQVYHLIDNHSCIQTAVSHLLRNHQALFKYLCDYLRSGEFPL 170


>SPAC23C4.19 |spt5||transcription elongation factor
           Spt5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 990

 Score = 27.9 bits (59), Expect = 1.7
 Identities = 18/41 (43%), Positives = 23/41 (56%), Gaps = 1/41 (2%)
 Frame = -1

Query: 233 VQRVVQHRLG-VRTHVDSDG*TLMRLDASERGVEREFAHRD 114
           V  VV++  G V T V SDG   +RLD   RG+ + F H D
Sbjct: 488 VSGVVENVRGSVITMVSSDG---LRLDVPSRGLRKRFRHGD 525


>SPAC25B8.13c |isp7||2-OG-Fe|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 397

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 13/27 (48%), Positives = 19/27 (70%)
 Frame = -1

Query: 215 HRLGVRTHVDSDG*TLMRLDASERGVE 135
           +RLGV+ H D+D  TLM  D + +G+E
Sbjct: 269 NRLGVQEHTDADALTLMSQD-NVKGLE 294


>SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 6.7
 Identities = 12/21 (57%), Positives = 13/21 (61%)
 Frame = -3

Query: 90  RPGPKYVLRRLQQWRGRPSPA 28
           R  PK  LRR +QW  R SPA
Sbjct: 85  RDSPKIWLRRERQWLQRHSPA 105


>SPBC29A10.15 |orc1|orp1, cdc30|origin recognition complex subunit
           Orc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 707

 Score = 25.4 bits (53), Expect = 8.9
 Identities = 9/27 (33%), Positives = 17/27 (62%)
 Frame = +2

Query: 131 RALHRARRHQASSVSTHHYRRGYEHPV 211
           R  H+  RH +S++++   R GY+ P+
Sbjct: 276 RRRHKRSRHNSSNLASTPKRNGYKQPL 302


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,717,486
Number of Sequences: 5004
Number of extensions: 49746
Number of successful extensions: 171
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 165
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 363302114
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)