Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00271
(758 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 138 4e-33
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 138 4e-33
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 136 2e-32
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 132 2e-31
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 106 1e-23
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 103 1e-22
At2g32250.2 68415.m03942 far-red impaired responsive protein, pu... 32 0.36
At2g32250.1 68415.m03941 far-red impaired responsive protein, pu... 32 0.36
At4g23790.1 68417.m03421 expressed protein many other Arabidopsi... 31 1.1
At1g78750.1 68414.m09178 F-box family protein contains F-box dom... 30 1.9
At2g29650.2 68415.m03604 inorganic phosphate transporter, putati... 29 2.5
At2g29650.1 68415.m03603 inorganic phosphate transporter, putati... 29 2.5
At5g56730.1 68418.m07080 peptidase M16 family protein / insulina... 28 7.7
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 138 bits (334), Expect = 4e-33
Identities = 60/83 (72%), Positives = 72/83 (86%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ DVLKNWPE +++ IVVTDGERILGLGDLG GMGIPVGKLALY+ALGG++P CLP+T
Sbjct: 160 ILDVLKNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVT 219
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L+D YIGLRQ+R
Sbjct: 220 IDVGTNNEKLLNDEFYIGLRQKR 242
Score = 77.4 bits (182), Expect = 1e-14
Identities = 36/82 (43%), Positives = 49/82 (59%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y ELL+EFM AV + YG+ L+QFEDF N NAF LL KY + + FNDDIQ
Sbjct: 245 GQEYSELLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVV 304
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+ +LT L+++ +F
Sbjct: 305 LAGLVSAQKLTNSPLAEHTFLF 326
Score = 33.5 bits (73), Expect = 0.16
Identities = 13/22 (59%), Positives = 19/22 (86%)
Frame = +1
Query: 580 QEARCRIWMVDSKGLIVKNRPE 645
+E+R +IW+VDSKGLIV +R +
Sbjct: 353 EESRKKIWLVDSKGLIVNSRKD 374
Score = 31.1 bits (67), Expect = 0.83
Identities = 11/32 (34%), Positives = 22/32 (68%)
Frame = +3
Query: 663 KERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
K+ +A +H PV+ L ++ +P+VLIG++ +
Sbjct: 380 KKPWAHEHEPVKDLLGAIKAIKPTVLIGSSGV 411
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 138 bits (334), Expect = 4e-33
Identities = 59/83 (71%), Positives = 73/83 (87%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+++VL+NWPE +++ IVVTDGERILGLGDLG GMGIPVGKL+LYTALGG++P CLP+T
Sbjct: 225 IHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVT 284
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L+D YIGLRQRR
Sbjct: 285 IDVGTNNEKLLNDEFYIGLRQRR 307
Score = 73.3 bits (172), Expect = 2e-13
Identities = 36/82 (43%), Positives = 46/82 (56%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y EL+ EFM AV + YG+ ++QFEDF N NAF LL KY + FNDDIQ
Sbjct: 310 GEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVV 369
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+LR LSD+ +F
Sbjct: 370 LAGLIAALRFVGGSLSDHRFLF 391
Score = 36.7 bits (81), Expect = 0.017
Identities = 16/24 (66%), Positives = 19/24 (79%)
Frame = +1
Query: 574 PEQEARCRIWMVDSKGLIVKNRPE 645
P +EAR IW+VDSKGLIV +R E
Sbjct: 416 PLEEARKNIWLVDSKGLIVSSRKE 439
Score = 35.5 bits (78), Expect = 0.039
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +3
Query: 663 KERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
K+ +A DH P+R L + V+ +P+VLIG + +
Sbjct: 445 KKPWAHDHEPIRELVDAVKAIKPTVLIGTSGV 476
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 136 bits (328), Expect = 2e-32
Identities = 60/83 (72%), Positives = 71/83 (85%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ +VLKNWP+ ++ IVVTDGERILGLGDLG GMGIPVGKL+LYTALGGI+P CLPIT
Sbjct: 167 ILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPIT 226
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L+D YIGL+QRR
Sbjct: 227 IDVGTNNEKLLNDEFYIGLKQRR 249
Score = 74.5 bits (175), Expect = 7e-14
Identities = 36/82 (43%), Positives = 48/82 (58%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y E L EFM AV + YG+ LVQFEDF N NAF LL KY + + FNDDIQ
Sbjct: 252 GQEYAEFLHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVV 311
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+ ++ K+L+D+ +F
Sbjct: 312 LAGLIAAQKVLGKKLADHTFLF 333
Score = 37.9 bits (84), Expect = 0.007
Identities = 17/29 (58%), Positives = 20/29 (68%)
Frame = +1
Query: 559 KGEGTPEQEARCRIWMVDSKGLIVKNRPE 645
K G P E R +IW+VDSKGLIV +R E
Sbjct: 353 KETGAPITETRKKIWLVDSKGLIVSSRKE 381
Score = 31.1 bits (67), Expect = 0.83
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +3
Query: 663 KERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
K+ +A +H PV+ L V +P+VLIG + +
Sbjct: 387 KQPWAHEHKPVKDLIGAVNAIKPTVLIGTSGV 418
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 132 bits (320), Expect = 2e-31
Identities = 58/83 (69%), Positives = 71/83 (85%)
Frame = +3
Query: 6 VYDVLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPIT 185
+ +VLKNWP+ ++ IVVTDGERILGLGDLG GMGIPVGKL+LYTALGGI+P CLPIT
Sbjct: 167 ILEVLKNWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPIT 226
Query: 186 IDVGTNTQSMLDDPLYIGLRQRR 254
IDVGTN + +L++ YIGL+Q+R
Sbjct: 227 IDVGTNNEKLLNNEFYIGLKQKR 249
Score = 71.7 bits (168), Expect = 5e-13
Identities = 35/82 (42%), Positives = 47/82 (57%)
Frame = +2
Query: 260 GPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXXXX 439
G +Y E L EFM AV + YG+ LVQFEDF N +AF LL KY + + FNDDIQ
Sbjct: 252 GEEYAEFLQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVV 311
Query: 440 XXXXXXSLRLTQKRLSDNVIVF 505
+ ++ K L+D+ +F
Sbjct: 312 LAGLIAAQKVLGKSLADHTFLF 333
Score = 37.5 bits (83), Expect = 0.010
Identities = 17/29 (58%), Positives = 19/29 (65%)
Frame = +1
Query: 559 KGEGTPEQEARCRIWMVDSKGLIVKNRPE 645
K G P E R +IW+VDSKGLIV R E
Sbjct: 353 KETGKPIDETRKKIWLVDSKGLIVSERKE 381
Score = 33.1 bits (72), Expect = 0.21
Identities = 13/32 (40%), Positives = 20/32 (62%)
Frame = +3
Query: 663 KERFAQDHAPVRTLAEVVEVARPSVLIGAAAI 758
K+ +A DH PV+ L V +P+VLIG + +
Sbjct: 387 KQPWAHDHKPVKELLAAVNAIKPTVLIGTSGV 418
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 106 bits (255), Expect = 1e-23
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = +3
Query: 24 NWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTN 203
NWP V IVVTDG RILGLGDLG G+GI VGKL LY A GI P + LP+ IDVGTN
Sbjct: 180 NWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTN 239
Query: 204 TQSMLDDPLYIGLRQRR 254
+ + +DP+Y+GL+QRR
Sbjct: 240 NEKLRNDPMYLGLQQRR 256
Score = 58.4 bits (135), Expect = 5e-09
Identities = 28/56 (50%), Positives = 39/56 (69%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQ 421
L DY +++DEFM AV R+ + +VQFEDF + AF+LL++YR Y FNDD+Q
Sbjct: 257 LEDDDYIDVIDEFMEAVYTRW-PHVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQ 311
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 103 bits (247), Expect = 1e-22
Identities = 45/80 (56%), Positives = 58/80 (72%)
Frame = +3
Query: 15 VLKNWPETDVRAIVVTDGERILGLGDLGACGMGIPVGKLALYTALGGIKPHQCLPITIDV 194
++ NWP V IV+TDG RILGLGDLG G+GIP+GKL +Y A GI P + LPI +DV
Sbjct: 170 MIYNWPAPQVDMIVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDV 229
Query: 195 GTNTQSMLDDPLYIGLRQRR 254
GTN + +L + LY+G+RQ R
Sbjct: 230 GTNNEKLLQNDLYLGVRQPR 249
Score = 63.7 bits (148), Expect = 1e-10
Identities = 33/81 (40%), Positives = 46/81 (56%)
Frame = +2
Query: 254 LRGPDYDELLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYRNKYCTFNDDIQXXXX 433
L G +Y E++DEFM A R+ + +VQFEDF AF LE+YR K+C FNDD+Q
Sbjct: 250 LEGEEYLEIIDEFMEAAFTRWPK-AVVQFEDFQAKWAFGTLERYRKKFCMFNDDVQGTAG 308
Query: 434 XXXXXXXXSLRLTQKRLSDNV 496
++R + +SD V
Sbjct: 309 VALAGLLGTVRAQGRPISDFV 329
>At2g32250.2 68415.m03942 far-red impaired responsive protein,
putative similar to far-red impaired response protein
FAR1 [Arabidopsis thaliana] gi|5764395|gb|AAD51282;
contains Pfam:PF03101 domain: FAR1 family
Length = 805
Score = 32.3 bits (70), Expect = 0.36
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +2
Query: 191 RGYEHPVDAGRPAVHRAPAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFE-DFGNANAF 367
RG P AG A+ + AL D LL+ FM ++ G V F+ D N F
Sbjct: 146 RGKNKP--AGALAIKKGLQLALEEEDLKLLLEHFMEMQDKQPGFFYAVDFDSDKRVRNVF 203
Query: 368 RLLEKYRNKYCTFND 412
L K ++ YC+F+D
Sbjct: 204 WLDAKAKHDYCSFSD 218
>At2g32250.1 68415.m03941 far-red impaired responsive protein,
putative similar to far-red impaired response protein
FAR1 [Arabidopsis thaliana] gi|5764395|gb|AAD51282;
contains Pfam:PF03101 domain: FAR1 family
Length = 807
Score = 32.3 bits (70), Expect = 0.36
Identities = 25/75 (33%), Positives = 35/75 (46%), Gaps = 1/75 (1%)
Frame = +2
Query: 191 RGYEHPVDAGRPAVHRAPAAALRGPDYDELLDEFMRAVVRRYGQNCLVQFE-DFGNANAF 367
RG P AG A+ + AL D LL+ FM ++ G V F+ D N F
Sbjct: 146 RGKNKP--AGALAIKKGLQLALEEEDLKLLLEHFMEMQDKQPGFFYAVDFDSDKRVRNVF 203
Query: 368 RLLEKYRNKYCTFND 412
L K ++ YC+F+D
Sbjct: 204 WLDAKAKHDYCSFSD 218
>At4g23790.1 68417.m03421 expressed protein many other Arabidopsis
putative proteins
Length = 430
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = +1
Query: 601 WMVDSKGLIVKNRPEEDLTFTRRDSLRTTRPSGPWLKLWKWPDRRCLLER 750
W+ DS G I N+ L ++ + RP +L WKW CLL R
Sbjct: 86 WIPDSVGPIYTNKSCGSLIDGHQNCITNGRPDLDFL-YWKWKPHDCLLPR 134
>At1g78750.1 68414.m09178 F-box family protein contains F-box domain
Pfam:PF00646
Length = 458
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = -2
Query: 379 LEEPKSVSVPEVLELHETVLPVAAHDGAHELVEQLVVV 266
L PK VS+P + ELH +++ A H L+ Q V+
Sbjct: 165 LASPKFVSLPSLKELHLSIVKFADHMALETLISQCPVL 202
>At2g29650.2 68415.m03604 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 398
Score = 29.5 bits (63), Expect = 2.5
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +1
Query: 586 ARCRIWMVDSKGLIVKNRPEEDLTFTRRDSLRTTRPSGPWLKLWKWPDRR 735
AR + + L NRP E + +R SLR +PS L++W +P R
Sbjct: 3 ARALLCSSNIHSLYTSNRPPEKTSSSR--SLRNLKPSPKSLRVWIYPRNR 50
>At2g29650.1 68415.m03603 inorganic phosphate transporter, putative
similar to brain specific Na+-dependent inorganic
phosphate cotransporter [Rattus norvegicus] GI:507415;
contains Pfam profile PF00083: major facilitator
superfamily protein
Length = 512
Score = 29.5 bits (63), Expect = 2.5
Identities = 17/50 (34%), Positives = 25/50 (50%)
Frame = +1
Query: 586 ARCRIWMVDSKGLIVKNRPEEDLTFTRRDSLRTTRPSGPWLKLWKWPDRR 735
AR + + L NRP E + +R SLR +PS L++W +P R
Sbjct: 3 ARALLCSSNIHSLYTSNRPPEKTSSSR--SLRNLKPSPKSLRVWIYPRNR 50
>At5g56730.1 68418.m07080 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 956
Score = 27.9 bits (59), Expect = 7.7
Identities = 21/82 (25%), Positives = 38/82 (46%)
Frame = -2
Query: 439 RHGRRPLDVVVESAVLVPILLEEPKSVSVPEVLELHETVLPVAAHDGAHELVEQLVVVGA 260
R+ + + + AV V +LEE V ++E H + H++V+ L +GA
Sbjct: 55 RNSKPRMRAALALAVKVGSVLEEEDQRGVAHIVE-HLAFSATTRYTN-HDIVKFLESIGA 112
Query: 259 AQRRCRSPMYSGSSSIDWVFVP 194
C++ M + +I +FVP
Sbjct: 113 EFGPCQNAMTTADETIYELFVP 134
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,932,562
Number of Sequences: 28952
Number of extensions: 293256
Number of successful extensions: 992
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 950
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 991
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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