BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00270 (662 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 114 9e-27 SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 114 9e-27 SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 29 0.60 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 27 2.4 SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharom... 27 3.2 SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Ph... 25 7.4 SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit ... 25 9.7 SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 25 9.7 >SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 114 bits (275), Expect = 9e-27 Identities = 52/73 (71%), Positives = 61/73 (83%) Frame = +3 Query: 36 KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKT 215 KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++KT Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKKT 76 Query: 216 RIIDVVYNASNNE 254 RII V Y+ SNNE Sbjct: 77 RIIQVAYHPSNNE 89 Score = 71.7 bits (168), Expect = 9e-14 Identities = 40/84 (47%), Positives = 51/84 (60%) Frame = +2 Query: 254 MVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKY 433 +VRT TL K+AIV +DA PFR WYE+HY + +G KG K T +S+ RK+ Sbjct: 90 LVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATSTP-----NPKSKHVQRKH 143 Query: 434 LARQRLAKVEGALEEQFHTGRLLA 505 AR +KV+ ALE QF GRL A Sbjct: 144 SARLGDSKVDSALETQFAAGRLYA 167 Score = 52.8 bits (121), Expect = 4e-08 Identities = 22/38 (57%), Positives = 29/38 (76%) Frame = +1 Query: 490 GAFAGLLASRPGQCGRADGYILEGKELEFYLRKIKSKR 603 G +++SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 163 GRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosaccharomyces pombe|chr 1|||Manual Length = 200 Score = 114 bits (275), Expect = 9e-27 Identities = 52/73 (71%), Positives = 61/73 (83%) Frame = +3 Query: 36 KRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKT 215 KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRLD+GNFSWGSE ++KT Sbjct: 17 KRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRLDSGNFSWGSEGVSKKT 76 Query: 216 RIIDVVYNASNNE 254 RII V Y+ SNNE Sbjct: 77 RIIQVAYHPSNNE 89 Score = 70.9 bits (166), Expect = 1e-13 Identities = 40/84 (47%), Positives = 51/84 (60%) Frame = +2 Query: 254 MVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKY 433 +VRT TL K+AIV +DA PFR WYE+HY + +G KG K T +S+ RK+ Sbjct: 90 LVRTNTLTKSAIVQIDAAPFRVWYETHYGILMG-SKGKKATATP-----TPKSKHVQRKH 143 Query: 434 LARQRLAKVEGALEEQFHTGRLLA 505 AR +KV+ ALE QF GRL A Sbjct: 144 SARLGDSKVDSALETQFAAGRLYA 167 Score = 52.8 bits (121), Expect = 4e-08 Identities = 22/38 (57%), Positives = 29/38 (76%) Frame = +1 Query: 490 GAFAGLLASRPGQCGRADGYILEGKELEFYLRKIKSKR 603 G +++SRPGQ GR DGYILEG+EL FYLR++ K+ Sbjct: 163 GRLYAVVSSRPGQSGRCDGYILEGEELHFYLRRMAPKK 200 >SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|||Manual Length = 207 Score = 29.1 bits (62), Expect = 0.60 Identities = 16/50 (32%), Positives = 25/50 (50%) Frame = +3 Query: 15 SEGPPDGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 164 S+G K+ +KRK L R A RL + + S+ S+GG +A + Sbjct: 35 SKGVKTNKKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.1 bits (57), Expect = 2.4 Identities = 17/51 (33%), Positives = 24/51 (47%) Frame = +3 Query: 69 ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKTRI 221 EL +N + Q+ + S T YRA+ LDTG+ E + K RI Sbjct: 1108 ELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDTGDLMAVKEVALHKPRI 1158 >SPCC132.01c ||SPCC1322.17c|DUF814 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1021 Score = 26.6 bits (56), Expect = 3.2 Identities = 16/68 (23%), Positives = 31/68 (45%) Frame = +2 Query: 302 ATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKYLARQRLAKVEGALEEQ 481 +TP + H KKG+K+ A++ ++R + AR+ A + K ++E+ Sbjct: 754 STPDTTGSDIHIVSEKRGKKGSKVITAKKVSAKERREARRARRQTALEESLKAPISIEDA 813 Query: 482 FHTGRLLA 505 +LA Sbjct: 814 TDPQTILA 821 >SPAC30D11.08c |phf2|swp2, saf60|PHD finger containing protein Phf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 538 Score = 25.4 bits (53), Expect = 7.4 Identities = 13/29 (44%), Positives = 16/29 (55%) Frame = -2 Query: 247 LDALYTTSMIRVLRVEHSDPQEKLPVSRR 161 L+ + T S +V R H DP KLP RR Sbjct: 193 LEGVKTKSGRKVHRPNHFDPLVKLPTRRR 221 >SPAC24C9.07c |bgs2|meu21, pgs2|1,3-beta-glucan synthase subunit Bgs2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1894 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/32 (34%), Positives = 16/32 (50%) Frame = +1 Query: 94 PGSVLSVSTPFVHVVEILSTVRCVWTPVTSLG 189 P VLS P++ + L + C+W P LG Sbjct: 682 PILVLSTMKPYLCNITFLGSHLCIWQPKILLG 713 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 25.0 bits (52), Expect = 9.7 Identities = 11/27 (40%), Positives = 13/27 (48%) Frame = -3 Query: 540 GATTLTWATRQQASKRPVWNCSSRAPS 460 G+ T W SK P WN SR P+ Sbjct: 833 GSRTPAW---NSGSKTPAWNSGSRTPA 856 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,495,510 Number of Sequences: 5004 Number of extensions: 48456 Number of successful extensions: 138 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 127 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 136 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 301829700 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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