BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00270 (662 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 110 7e-25 At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 109 1e-24 At2g22830.1 68415.m02711 squalene monooxygenase, putative / squa... 30 1.6 At1g73260.1 68414.m08478 trypsin and protease inhibitor family p... 28 4.8 >At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal protein S8 - Zea mays, PIR:T04088 Length = 222 Score = 110 bits (265), Expect = 7e-25 Identities = 50/75 (66%), Positives = 61/75 (81%), Gaps = 1/75 (1%) Frame = +3 Query: 33 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 209 GK+ RKKRKYE+GR ANT+L + + +R RGGN K+RALRLDTGN+SWGSE +TR Sbjct: 16 GKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNYSWGSEATTR 75 Query: 210 KTRIIDVVYNASNNE 254 KTR++DVVYNASNNE Sbjct: 76 KTRVLDVVYNASNNE 90 Score = 82.2 bits (194), Expect = 3e-16 Identities = 48/95 (50%), Positives = 60/95 (63%), Gaps = 10/95 (10%) Frame = +2 Query: 254 MVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGR-KKGAKLT-----EAEEAII----NK 403 +VRTKTLVK+AIV VDA PF+QWY SHY + LGR KK A T E EEA + Sbjct: 91 LVRTKTLVKSAIVQVDAAPFKQWYLSHYGVELGRKKKSASSTKKDGEEGEEAAVAAPEEV 150 Query: 404 KRSQKTARKYLARQRLAKVEGALEEQFHTGRLLAC 508 K+S RK +RQ ++ +E+QF +GRLLAC Sbjct: 151 KKSNHLLRKIASRQEGRSLDSHIEDQFASGRLLAC 185 Score = 65.7 bits (153), Expect = 3e-11 Identities = 27/40 (67%), Positives = 33/40 (82%) Frame = +1 Query: 490 GAFAGLLASRPGQCGRADGYILEGKELEFYLRKIKSKRAK 609 G ++SRPGQCGRADGYILEGKELEFY++KI+ K+ K Sbjct: 180 GRLLACISSRPGQCGRADGYILEGKELEFYMKKIQKKKGK 219 >At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ribosomal protein S8, Prunus armeniaca, EMBL:AF071889 Length = 210 Score = 109 bits (263), Expect = 1e-24 Identities = 52/75 (69%), Positives = 59/75 (78%), Gaps = 1/75 (1%) Frame = +3 Query: 33 GKRAPIRKKRKYELGRPAANTRLGPQR-IHSVRSRGGNTKYRALRLDTGNFSWGSECSTR 209 GK+ RKKRKYELGR ANT+L + + +R RGGN K+RALRLDTGNFSWGSE TR Sbjct: 16 GKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWRALRLDTGNFSWGSEAVTR 75 Query: 210 KTRIIDVVYNASNNE 254 KTRI+DV YNASNNE Sbjct: 76 KTRILDVAYNASNNE 90 Score = 78.2 bits (184), Expect = 5e-15 Identities = 42/85 (49%), Positives = 53/85 (62%) Frame = +2 Query: 254 MVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRKKGAKLTEAEEAIINKKRSQKTARKY 433 +VRT+TLVK+AIV VDA PF+Q Y HY + +GRKK + EE K+S RK Sbjct: 91 LVRTQTLVKSAIVQVDAAPFKQGYLQHYGVDIGRKKKGEAVTTEEV----KKSNHVQRKL 146 Query: 434 LARQRLAKVEGALEEQFHTGRLLAC 508 RQ ++ LEEQF +GRLLAC Sbjct: 147 EMRQEGRALDSHLEEQFSSGRLLAC 171 Score = 66.1 bits (154), Expect = 2e-11 Identities = 27/40 (67%), Positives = 33/40 (82%) Frame = +1 Query: 490 GAFAGLLASRPGQCGRADGYILEGKELEFYLRKIKSKRAK 609 G +ASRPGQCGRADGYILEGKELEFY++K++ K+ K Sbjct: 166 GRLLACIASRPGQCGRADGYILEGKELEFYMKKLQKKKGK 205 >At2g22830.1 68415.m02711 squalene monooxygenase, putative / squalene epoxidase, putative similar to SP|O65404 (SE 1,1), SP|O65402 (SE 1,2), SP|O65403 (SE 2) Length = 585 Score = 29.9 bits (64), Expect = 1.6 Identities = 15/49 (30%), Positives = 23/49 (46%) Frame = -3 Query: 333 CDSYHCLNGVASTTTIAFLTRVFVRTIHC*MHYTQHQ*YGFCELNIPIP 187 C Y L GV S+ +A L+ + R + +H+ Y C L +P P Sbjct: 492 CFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPFP 540 >At1g73260.1 68414.m08478 trypsin and protease inhibitor family protein / Kunitz family protein similar to trypsin inhibitor propeptide [Brassica oleracea] GI:841208; contains Pfam profile PF00197: Trypsin and protease inhibitor Length = 215 Score = 28.3 bits (60), Expect = 4.8 Identities = 17/41 (41%), Positives = 22/41 (53%), Gaps = 1/41 (2%) Frame = +2 Query: 263 TKTLVKNAI-VVVDATPFRQWYESHYTLPLGRKKGAKLTEA 382 T L NA VVD ++ES+Y LP+ R +G LT A Sbjct: 19 TAVLASNAYGAVVDIDGNAMFHESYYVLPVIRGRGGGLTLA 59 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,369,624 Number of Sequences: 28952 Number of extensions: 263690 Number of successful extensions: 760 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 738 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 756 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1393347168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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