Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00268
(508 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A) ribo... 115 2e-26
At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B) 112 1e-25
At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B) 112 1e-25
At5g08440.1 68418.m00994 expressed protein 29 2.4
At5g51860.1 68418.m06429 MADS-box protein (AGL72) contains Pfam ... 28 4.2
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 5.5
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 27 7.3
At4g14740.2 68417.m02267 expressed protein 27 9.6
At4g14740.1 68417.m02266 expressed protein 27 9.6
At3g22810.1 68416.m02875 expressed protein ; expression support... 27 9.6
>At4g18100.1 68417.m02692 60S ribosomal protein L32 (RPL32A)
ribosomal protein L32, human, PIR1:R5HU32
Length = 133
Score = 115 bits (277), Expect = 2e-26
Identities = 54/74 (72%), Positives = 61/74 (82%)
Frame = +2
Query: 254 SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 433
S+KKTRH LPNGF+K +VHN ELE+LMM NR YCAEIAH VS+KKRK IVERA QL +
Sbjct: 60 SDKKTRHYLPNGFKKFVVHNTSELELLMMHNRTYCAEIAHNVSTKKRKAIVERASQLDVV 119
Query: 434 VTNAAARLRSQENE 475
VTN ARLRSQE+E
Sbjct: 120 VTNRLARLRSQEDE 133
Score = 74.5 bits (175), Expect = 4e-14
Identities = 31/60 (51%), Positives = 44/60 (73%)
Frame = +3
Query: 87 PVYRPTIXXKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGPTRR 266
P+ + KR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG ++
Sbjct: 4 PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYGSDKK 63
>At5g46430.2 68418.m05716 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 112 bits (269), Expect = 1e-25
Identities = 51/74 (68%), Positives = 60/74 (81%)
Frame = +2
Query: 254 SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 433
S+KKTRH LPNGF+K +VHN ELE+LMM NR YCAEIAH +S+KKRK IVERA QL +
Sbjct: 60 SDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVV 119
Query: 434 VTNAAARLRSQENE 475
V+N RLRSQE+E
Sbjct: 120 VSNKLGRLRSQEDE 133
Score = 74.5 bits (175), Expect = 4e-14
Identities = 31/60 (51%), Positives = 44/60 (73%)
Frame = +3
Query: 87 PVYRPTIXXKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGPTRR 266
P+ + KR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG ++
Sbjct: 4 PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYGSDKK 63
>At5g46430.1 68418.m05715 60S ribosomal protein L32 (RPL32B)
Length = 133
Score = 112 bits (269), Expect = 1e-25
Identities = 51/74 (68%), Positives = 60/74 (81%)
Frame = +2
Query: 254 SNKKTRHMLPNGFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIR 433
S+KKTRH LPNGF+K +VHN ELE+LMM NR YCAEIAH +S+KKRK IVERA QL +
Sbjct: 60 SDKKTRHYLPNGFKKFIVHNTSELELLMMHNRTYCAEIAHNISTKKRKAIVERASQLDVV 119
Query: 434 VTNAAARLRSQENE 475
V+N RLRSQE+E
Sbjct: 120 VSNKLGRLRSQEDE 133
Score = 74.5 bits (175), Expect = 4e-14
Identities = 31/60 (51%), Positives = 44/60 (73%)
Frame = +3
Query: 87 PVYRPTIXXKRTKRFIRHQSDRYDKLKRNWRKPRGIDNRVRRRFKGQYLMPNIGYGPTRR 266
P+ + KR+ +FIR QSDR +K +WR+P+GID+RVRR+FKG LMPN+GYG ++
Sbjct: 4 PLLTKKVVKKRSAKFIRPQSDRRITVKESWRRPKGIDSRVRRKFKGVTLMPNVGYGSDKK 63
>At5g08440.1 68418.m00994 expressed protein
Length = 726
Score = 28.7 bits (61), Expect = 2.4
Identities = 17/71 (23%), Positives = 37/71 (52%), Gaps = 9/71 (12%)
Frame = +2
Query: 227 ILDAQHRLRSNKKTRHMLPNG--------FRKVLVHNVKELEILMMQNRKYCAEIA-HGV 379
+L+ H L N + ++P G +++ L+H V+E + +++ RKY A+ + V
Sbjct: 186 LLEGDHDLHINSSSHELMPVGEVNNSGTAWKQELIHKVQEQDQEILRLRKYLADYSTKEV 245
Query: 380 SSKKRKLIVER 412
+ K ++E+
Sbjct: 246 QIRNEKYVLEK 256
>At5g51860.1 68418.m06429 MADS-box protein (AGL72) contains Pfam
profile PF00319: SRF-type transcription factor
(DNA-binding and dimerisation domain);
Length = 211
Score = 27.9 bits (59), Expect = 4.2
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = +2
Query: 287 GFRKVLVHNVKELEILMMQNRKYCAEIAHGVSSKKRKLIVERAQQLSIRVTNAAARLRSQ 466
G +K +V VK++E+L + NRK + S K+ I + ++ V A+L
Sbjct: 90 GLKKEMVTMVKKIEVLEVHNRKMMGQSLDSCSVKELSEIATQIEKSLHMVRLRKAKLYED 149
Query: 467 E 469
E
Sbjct: 150 E 150
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.5 bits (58), Expect = 5.5
Identities = 15/48 (31%), Positives = 25/48 (52%), Gaps = 4/48 (8%)
Frame = +3
Query: 57 LKKHT-RWTIR---PVYRPTIXXKRTKRFIRHQSDRYDKLKRNWRKPR 188
L+ H WT R P + + +K +++H + R DKL++ KPR
Sbjct: 183 LRNHRDTWTFRAVDPNSGTAVLMEASKSYLQHIAQRLDKLQKRGWKPR 230
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam domain
PF04931: DNA polymerase V
Length = 1306
Score = 27.1 bits (57), Expect = 7.3
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +2
Query: 296 KVLVHNVKELEILMMQNRKYCAEIAHGVSSKK 391
K LV N+ E ++ Q RK+C I VSS K
Sbjct: 1242 KELVGNMPEAKVRRAQVRKFCGRIFQMVSSLK 1273
>At4g14740.2 68417.m02267 expressed protein
Length = 475
Score = 26.6 bits (56), Expect = 9.6
Identities = 13/38 (34%), Positives = 17/38 (44%), Gaps = 2/38 (5%)
Frame = -1
Query: 466 LGPEASGRIRHSDAELLGSFHDQLPLL--RRDTVSDLC 359
+ P SGR+ HS L GS D P+ D + C
Sbjct: 107 VSPRTSGRLSHSSGPLNGSLTDSPPVSPPESDDIKQFC 144
>At4g14740.1 68417.m02266 expressed protein
Length = 475
Score = 26.6 bits (56), Expect = 9.6
Identities = 13/38 (34%), Positives = 17/38 (44%), Gaps = 2/38 (5%)
Frame = -1
Query: 466 LGPEASGRIRHSDAELLGSFHDQLPLL--RRDTVSDLC 359
+ P SGR+ HS L GS D P+ D + C
Sbjct: 107 VSPRTSGRLSHSSGPLNGSLTDSPPVSPPESDDIKQFC 144
>At3g22810.1 68416.m02875 expressed protein ; expression supported
by MPSS
Length = 472
Score = 26.6 bits (56), Expect = 9.6
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -1
Query: 466 LGPEASGRIRHSDAELLGSFHDQLPL 389
+ P SGR+ HS L GS D P+
Sbjct: 106 VSPRTSGRLSHSSGPLNGSLTDSPPI 131
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,505,140
Number of Sequences: 28952
Number of extensions: 206473
Number of successful extensions: 657
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 637
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 657
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 908059136
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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