Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00264
(643 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC806.07 |ndk1||nucleoside diphosphate kinase|Schizosaccharomy... 116 2e-27
SPBC428.14 |||1-acylglycerol-3-phosphate acyltransferase |Schizo... 27 1.7
SPCC1235.12c |mug146||meiotically upregulated gene Mug46|Schizos... 27 2.3
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 27 2.3
SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyc... 26 4.0
SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 26 4.0
SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces pomb... 26 4.0
SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo... 26 4.0
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 5.3
SPAC8E11.04c |||phospholipase |Schizosaccharomyces pombe|chr 1||... 25 9.3
SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit L37|... 25 9.3
SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb... 25 9.3
>SPAC806.07 |ndk1||nucleoside diphosphate kinase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 151
Score = 116 bits (280), Expect = 2e-27
Identities = 51/82 (62%), Positives = 62/82 (75%)
Frame = +1
Query: 262 RPFFPGLVKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVGRNII 441
+PF+ LV +M+SGPV M+WEG VKTGR MLGA+NP DS PGTIRGD I +GRN+
Sbjct: 57 KPFYEKLVGFMASGPVCAMIWEGKQAVKTGRLMLGASNPLDSAPGTIRGDYGIDLGRNVC 116
Query: 442 HGSDSVESAKKEIGLWFTDKEV 507
HGSDS+ESA +EI LWF E+
Sbjct: 117 HGSDSIESANREIKLWFQPSEI 138
Score = 71.3 bits (167), Expect = 1e-13
Identities = 32/54 (59%), Positives = 43/54 (79%)
Frame = +2
Query: 104 ERTFIMVKPDGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDWHPG 265
E+TFI VKPD VQRGL+G II +FE KG+KL LKF+ PS +L+++HY++ H G
Sbjct: 4 EQTFIAVKPDAVQRGLIGYIISKFELKGYKLRALKFLVPSRDLVEEHYAE-HKG 56
>SPBC428.14 |||1-acylglycerol-3-phosphate acyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 350
Score = 27.5 bits (58), Expect = 1.7
Identities = 19/60 (31%), Positives = 28/60 (46%), Gaps = 2/60 (3%)
Frame = -1
Query: 235 EKFF*WPYE--FQTD*FEAFLFETFNNGAHKTTLYTIRLNHNKSTLTLFRHHEILLTCSI 62
++F W Y+ ++ D L ET N K T+RL H L+LF +L TC +
Sbjct: 283 DQFTDWLYQRWYEKDKLIDTLLETGNFPGPKKLHTTVRLKHRLEILSLF---SVLFTCIV 339
>SPCC1235.12c |mug146||meiotically upregulated gene
Mug46|Schizosaccharomyces pombe|chr 3|||Manual
Length = 311
Score = 27.1 bits (57), Expect = 2.3
Identities = 20/69 (28%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
Frame = -2
Query: 507 NFFVRKPEAYFLFSRFNAVRTMDDVTPNLNAEITADSAGLRVSWVSC-AKHLTASLHNIK 331
N FV P R + RT+ TP+L E ++ R+S V C H +S N
Sbjct: 5 NTFVDYPTTQVRKERVHTYRTLTSATPSL--EFFSNENTNRLSEVQCKLTHFLSSSENSS 62
Query: 330 ALPYHRDHR 304
++ R H+
Sbjct: 63 SVRNTRTHK 71
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 27.1 bits (57), Expect = 2.3
Identities = 10/48 (20%), Positives = 25/48 (52%)
Frame = +2
Query: 131 DGVQRGLVGTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDWHPGLSS 274
+ V + ++++ + K L+G + ++ ++Q + +DW LSS
Sbjct: 86 ENVLNSQIRFLLDKLDTKAKDLLGEEMIFELASIVQDYLNDWQSDLSS 133
>SPAC11G7.02 |pub1||ubiquitin-protein ligase E3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 767
Score = 26.2 bits (55), Expect = 4.0
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = +2
Query: 155 GTIIERFEKKGFKLVGLKFVWPSEELLQQHYSDWHPGLSSLV 280
G IE E+ + V L VW ++ +++ ++ +H G S L+
Sbjct: 588 GRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELI 629
>SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase
Ppk30|Schizosaccharomyces pombe|chr 2|||Manual
Length = 953
Score = 26.2 bits (55), Expect = 4.0
Identities = 14/52 (26%), Positives = 23/52 (44%)
Frame = -3
Query: 380 AGLVAPSI*RPVFTTLRPSHTIGTTGPELMYFTRPGKKGRDANRCSVAGEVL 225
+G + P RP T+L P T+ TT + RP K + + ++ L
Sbjct: 358 SGSLTPLSSRPAHTSLPPIPTVQTTSSNVPPVNRPSLKSKSPSVSNILSNQL 409
>SPBC1703.06 |pof10||F-box protein Pof10|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 662
Score = 26.2 bits (55), Expect = 4.0
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2
Query: 189 KPFFSKRSIMVPTRPRCTPSGLT 121
KPF +KRS ++P RP T L+
Sbjct: 512 KPFVNKRSKVLPLRPSVTHDNLS 534
>SPAC1039.07c |||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 448
Score = 26.2 bits (55), Expect = 4.0
Identities = 17/59 (28%), Positives = 33/59 (55%), Gaps = 4/59 (6%)
Frame = +1
Query: 280 LVKYMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQP--GTIRGDLCIQVGR--NIIH 444
L++ P++ V GL +++ G ++ T+P+ GT+ GD C+++G NI+H
Sbjct: 349 LLRLKDKHPLIVDV-RGLGLLQ-GIEIASCTDPSKPSDFLGTVIGDKCLELGMNCNIVH 405
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 5.3
Identities = 15/60 (25%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = -3
Query: 536 PVFICRCPAPTSLSVNQRPISFLADSTLSEPWMMLRPT*MQRSP-RIVPGCESAGLVAPS 360
P + PAP+S P+S L + + P + P+ Q+ P + P + + PS
Sbjct: 1463 PGMVTNAPAPSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLPPS 1522
>SPAC8E11.04c |||phospholipase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 224
Score = 25.0 bits (52), Expect = 9.3
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +1
Query: 235 PATLQRLASRPFFPGLVKYMSSGPVVPMV 321
P+ L R+A P L+ YM+ P+VP V
Sbjct: 150 PSALSRVAKE--IPILLTYMTEDPIVPSV 176
>SPCC645.09 |mrpl37||mitochondrial ribosomal protein subunit
L37|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 9.3
Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 2/65 (3%)
Frame = +1
Query: 241 TLQRLASRPFFPGLVK--YMSSGPVVPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDL 414
TL +SR LVK Y+SS + + +++ T+ + PGTI L
Sbjct: 27 TLVLRSSRNSSSSLVKRSYVSSRVSPKKPQHNSDATSSAQKVANKTHTSSVLPGTILKGL 86
Query: 415 CIQVG 429
CI+ G
Sbjct: 87 CIKAG 91
>SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 574
Score = 25.0 bits (52), Expect = 9.3
Identities = 17/70 (24%), Positives = 29/70 (41%), Gaps = 4/70 (5%)
Frame = +1
Query: 310 VPMVWEGLNVVKTGRQMLGATNPADSQPGTIRGDLCIQVGRNI----IHGSDSVESAKKE 477
+ V EG N + GRQ+L T+P + + +G I H SD + + +
Sbjct: 388 ITWVSEGANTMDRGRQLLEVTHPRGRLDAGTMSTMGVGMGYAIASAFAHSSDKIVVVEGD 447
Query: 478 IGLWFTDKEV 507
F+ E+
Sbjct: 448 SAFGFSAMEL 457
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,602,105
Number of Sequences: 5004
Number of extensions: 52483
Number of successful extensions: 134
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 287744314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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