BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00258 (746 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 31 0.17 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 26 0.77 SPCC417.08 |tef3||translation elongation factor eEF3|Schizosacch... 27 2.1 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 31.1 bits (67), Expect = 0.17 Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 1/52 (1%) Frame = +1 Query: 16 QEVSNGPDMGRKIPNRIPVRDENDCDTRAYIKDDSVKIVTLMSAPI-IPNSA 168 Q+ S P G ++PN +N+ + + +K+DS K SAPI +P S+ Sbjct: 28 QKKSISPSSGSELPNFKTTISQNNEEVKTSLKEDSSKFHPSASAPIFVPTSS 79 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 26.2 bits (55), Expect(2) = 0.77 Identities = 9/17 (52%), Positives = 12/17 (70%) Frame = -3 Query: 585 LTSEGKKSLTISWCPAD 535 LT K +L++SWCP D Sbjct: 256 LTGHQKAALSLSWCPED 272 Score = 21.0 bits (42), Expect(2) = 0.77 Identities = 7/14 (50%), Positives = 10/14 (71%) Frame = -3 Query: 552 SWCPADHNLSAYAN 511 SWCP++ N A A+ Sbjct: 310 SWCPSNSNRVAVAS 323 >SPCC417.08 |tef3||translation elongation factor eEF3|Schizosaccharomyces pombe|chr 3|||Manual Length = 1047 Score = 27.5 bits (58), Expect = 2.1 Identities = 17/53 (32%), Positives = 29/53 (54%) Frame = +1 Query: 52 IPNRIPVRDENDCDTRAYIKDDSVKIVTLMSAPIIPNSARDITRIVNERVGMV 210 +P IPV E+ DT+A +K S + +T + +I N+ DI R + E + + Sbjct: 173 LPQIIPVVSESMWDTKAEVKKQSKETMTKV-CTLIANA--DIDRFIPELINCI 222 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,849,371 Number of Sequences: 5004 Number of extensions: 55090 Number of successful extensions: 200 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 191 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 200 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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