BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00255 (640 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces p... 30 0.32 SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.32 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.43 SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizos... 28 0.99 SPAC222.11 |hem13||coproporphyrinogen III oxidase |Schizosacchar... 28 1.3 SPBC21C3.04c |||mitochondrial ribosomal protein subunit L34|Schi... 27 1.7 SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|... 27 3.0 SPBC359.03c |||amino acid permease, unknown 8|Schizosaccharomyce... 26 4.0 SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Sc... 26 4.0 SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase comp... 26 4.0 SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |S... 26 5.3 SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 5.3 SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc... 26 5.3 SPBC16G5.05c |||MSP domain|Schizosaccharomyces pombe|chr 2|||Manual 26 5.3 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 5.3 SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr ... 25 7.0 SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosa... 25 9.2 SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces p... 25 9.2 >SPAC22F8.08 |||COPII vesicle coat protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 926 Score = 29.9 bits (64), Expect = 0.32 Identities = 40/162 (24%), Positives = 58/162 (35%), Gaps = 9/162 (5%) Frame = +3 Query: 15 GGRPQHRQQPVDTRPPGQHTEAHSYRCHHADVPRRTIGCLRSSARQQLVSTRNSYSGANA 194 G P + VD +PP HT S R + A+V T S A L + SY A Sbjct: 67 GAAPTNIIGSVDQQPPVSHTSHKSRRQYPAEVFELTNTLAASPAPPSL--SEPSYVMART 124 Query: 195 ASAQVCSGSSGGAGGRPVQHXXXXXXXGFPHKSQHQRN----VQFPNSALTMAMGQQPPP 362 S+ G + + G + N V N ++ PPP Sbjct: 125 PSSSPYPHDRAEVGTKSLSAQFGGMSLGADGAAAMPTNELVSVDLYNQTPEISDLTSPPP 184 Query: 363 ----PLVRSTANTTTMNVPPQ-VTGTMNGHSTSQSVDTRKRL 473 PL S T N PP+ V T+N T+ S+ + ++ Sbjct: 185 PINLPLSYSATGAATSNCPPKYVRSTINCVPTTNSLLKKSKI 226 >SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 800 Score = 29.9 bits (64), Expect = 0.32 Identities = 16/51 (31%), Positives = 23/51 (45%) Frame = +3 Query: 354 PPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDTRKRLTPIPDINTTGN 506 P P V T+ ++T PP + + G S+S + T T I TGN Sbjct: 354 PVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLLSTSTSCTTSTSIPPTGN 404 Score = 28.7 bits (61), Expect = 0.75 Identities = 16/51 (31%), Positives = 22/51 (43%) Frame = +3 Query: 354 PPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDTRKRLTPIPDINTTGN 506 P P V T+ ++T PP + + G S+S T T I TGN Sbjct: 300 PVTPTVPPTSTSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGN 350 Score = 25.0 bits (52), Expect = 9.2 Identities = 13/50 (26%), Positives = 24/50 (48%), Gaps = 1/50 (2%) Frame = +3 Query: 354 PPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDTRKRLTP-IPDINTT 500 PPPP S+ T++ +P T S + ++ +TP +P +T+ Sbjct: 316 PPPPASTSSTGTSSSPLPSTSTSCTTSTSIPPTGNSTTPVTPTVPPTSTS 365 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 29.5 bits (63), Expect = 0.43 Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 2/54 (3%) Frame = +3 Query: 306 NVQF--PNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDT 461 NV F P+S L + +P P + N T ++ P T T GH T +V T Sbjct: 851 NVDFYEPHSYLE-SPAPEPQPSYEEESFNATVIHAPTPSTATFQGHPTISNVAT 903 >SPBC6B1.02 |ppk30||Ark1/Prk1 family protein kinase Ppk30|Schizosaccharomyces pombe|chr 2|||Manual Length = 953 Score = 28.3 bits (60), Expect = 0.99 Identities = 27/100 (27%), Positives = 43/100 (43%) Frame = +3 Query: 282 PHKSQHQRNVQFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDT 461 P ++ QR + L+ PP+ T TT+ NVPP ++ S S S Sbjct: 348 PPRAPLQRTPSGSLTPLSSRPAHTSLPPI--PTVQTTSSNVPPVNRPSLKSKSPSVSNIL 405 Query: 462 RKRLTPIPDINTTGNQRRIRYKRR*WKTQWCLASIRSPTS 581 +L+PI N R++ K T+ A+I++P S Sbjct: 406 SNQLSPISSAN-NDVMARLQPKSPIPATKSYSATIQTPRS 444 >SPAC222.11 |hem13||coproporphyrinogen III oxidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 312 Score = 27.9 bits (59), Expect = 1.3 Identities = 13/30 (43%), Positives = 15/30 (50%) Frame = +2 Query: 338 GDGSATPSTSCEEHGQHNNYERASASDRHD 427 G TPS EE G+H + A DRHD Sbjct: 139 GGADLTPSILFEEDGKHFHKLHKEACDRHD 168 >SPBC21C3.04c |||mitochondrial ribosomal protein subunit L34|Schizosaccharomyces pombe|chr 2|||Manual Length = 108 Score = 27.5 bits (58), Expect = 1.7 Identities = 11/36 (30%), Positives = 19/36 (52%) Frame = +3 Query: 420 GTMNGHSTSQSVDTRKRLTPIPDINTTGNQRRIRYK 527 GTM G T +T + +P +N G +++R+K Sbjct: 28 GTMRGFPTRNPFNTATLYSSLPTMNIFGQMQQVRWK 63 >SPBC1685.05 |||serine protease |Schizosaccharomyces pombe|chr 2|||Manual Length = 997 Score = 26.6 bits (56), Expect = 3.0 Identities = 11/26 (42%), Positives = 16/26 (61%) Frame = +3 Query: 171 NSYSGANAASAQVCSGSSGGAGGRPV 248 ++Y+ N Q SG+SGG+ G PV Sbjct: 210 DNYNDFNTFYYQAASGTSGGSSGSPV 235 >SPBC359.03c |||amino acid permease, unknown 8|Schizosaccharomyces pombe|chr 2|||Manual Length = 579 Score = 26.2 bits (55), Expect = 4.0 Identities = 14/50 (28%), Positives = 27/50 (54%) Frame = -1 Query: 508 WLPVVLMSGIGVSLLRVSTD*EVEWPFIVPVTCGGTFIVVVLAVLLTRGG 359 W+ + L+ IG++L V EVE+ + + T ++LA+++ GG Sbjct: 192 WISIFLVVVIGINLFGVRVFGEVEF-VLALIKVVATVGFIILAIIINCGG 240 >SPAC23E2.01 |fep1|gaf2|iron-sensing transcription factor Fep1|Schizosaccharomyces pombe|chr 1|||Manual Length = 564 Score = 26.2 bits (55), Expect = 4.0 Identities = 11/36 (30%), Positives = 22/36 (61%) Frame = +3 Query: 318 PNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGT 425 P S ++ + QQ P L++S+ + ++ PP+V G+ Sbjct: 463 PKSEVSPSQSQQAP--LIQSSTSPVSLQFPPEVQGS 496 >SPBP23A10.15c |qcr1|mas1|mitochondrial processing peptidase complex beta subunit Qcr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 457 Score = 26.2 bits (55), Expect = 4.0 Identities = 18/48 (37%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Frame = +1 Query: 448 NPWTLAKGLHRY-QTSTLLATNAV*GTKGVSGKHNGALHQFEALPVHG 588 N T+A H Y QT+T+L G++ + K+NGA H E L G Sbjct: 29 NGLTVATEHHPYAQTATVLVGVDA-GSRAETAKNNGAAHFLEHLAFKG 75 >SPAC14C4.03 |mek1||Cds1/Rad53/Chk2 family protein kinase Mek1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 445 Score = 25.8 bits (54), Expect = 5.3 Identities = 9/20 (45%), Positives = 13/20 (65%) Frame = +2 Query: 245 GTTRCHRSGRRIST*IAAPE 304 G RC + G+R+ST + PE Sbjct: 305 GVARCMQKGKRLSTFVGTPE 324 >SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccharomyces pombe|chr 2|||Manual Length = 884 Score = 25.8 bits (54), Expect = 5.3 Identities = 12/30 (40%), Positives = 16/30 (53%) Frame = +3 Query: 318 PNSALTMAMGQQPPPPLVRSTANTTTMNVP 407 P A T A+ PPPP VRS+ + + P Sbjct: 732 PTPAPTPAVKHHPPPPPVRSSISPSMPPAP 761 Score = 25.4 bits (53), Expect = 7.0 Identities = 11/21 (52%), Positives = 12/21 (57%) Frame = +1 Query: 256 LPSQRPPDFHINRSTRETFSF 318 LP Q D HI RS+ ET F Sbjct: 98 LPKQAKDDHHIKRSSSETADF 118 >SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1063 Score = 25.8 bits (54), Expect = 5.3 Identities = 14/38 (36%), Positives = 19/38 (50%) Frame = +3 Query: 21 RPQHRQQPVDTRPPGQHTEAHSYRCHHADVPRRTIGCL 134 +PQ QQP + P +TEA+ R VP R + L Sbjct: 167 QPQQTQQPQASSPTAPNTEANQQR--SGSVPGRIVPAL 202 >SPBC16G5.05c |||MSP domain|Schizosaccharomyces pombe|chr 2|||Manual Length = 383 Score = 25.8 bits (54), Expect = 5.3 Identities = 11/28 (39%), Positives = 15/28 (53%) Frame = +3 Query: 348 QQPPPPLVRSTANTTTMNVPPQVTGTMN 431 +QPP + NT+T N P V G+ N Sbjct: 127 KQPPQSADKQVENTSTSNPPVSVEGSEN 154 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 25.8 bits (54), Expect = 5.3 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +1 Query: 580 VHGAADDPAGPWQPLLPA 633 VHG + A P+QPL PA Sbjct: 1718 VHGVGAEMASPYQPLQPA 1735 >SPBC21B10.03c |||ataxin-2 homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 791 Score = 25.4 bits (53), Expect = 7.0 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = -1 Query: 517 IRRWLPVVLMSGIGVSLLRVSTD*EVEW 434 +RRW+P +G+ ++ L STD VEW Sbjct: 120 LRRWMPDPEDAGVPLAGLEESTD-NVEW 146 >SPBC2G2.01c |liz1|SPBC4B4.13c|pantothenate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 514 Score = 25.0 bits (52), Expect = 9.2 Identities = 17/49 (34%), Positives = 22/49 (44%), Gaps = 4/49 (8%) Frame = -3 Query: 485 WYR----CKPFASVHGLRG*VAIHRAGHLRRHVHSCCVGRAPHKRWRGL 351 WY+ CK A + G V AG+L+ VHS G+ WR L Sbjct: 148 WYKESELCKR-AGIFSASGLVGTMFAGYLQTAVHSSLNGKGGLSGWRWL 195 >SPAC4D7.10c |||SAGA complex subunit Spt20 |Schizosaccharomyces pombe|chr 1|||Manual Length = 473 Score = 25.0 bits (52), Expect = 9.2 Identities = 9/23 (39%), Positives = 12/23 (52%) Frame = +3 Query: 354 PPPPLVRSTANTTTMNVPPQVTG 422 PP P+ + AN + PP TG Sbjct: 357 PPKPMQKFPANAASSESPPNATG 379 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,690,056 Number of Sequences: 5004 Number of extensions: 54669 Number of successful extensions: 203 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 190 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 202 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 285732116 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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