Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00255
(640 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g49290.1 68416.m05387 expressed protein 34 0.069
At4g17950.1 68417.m02673 DNA-binding family protein contains Pfa... 33 0.21
At1g23420.1 68414.m02934 inner no outer protein (INO) identical ... 33 0.21
At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 fac... 30 1.5
At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 fac... 30 1.5
At5g64540.1 68418.m08111 hypothetical protein 29 2.0
At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein si... 29 2.6
At3g18810.1 68416.m02389 protein kinase family protein contains ... 29 2.6
At5g11460.1 68418.m01338 senescence-associated protein-related s... 28 4.5
At1g56530.1 68414.m06501 hydroxyproline-rich glycoprotein family... 28 4.5
At1g17440.2 68414.m02133 transcription initiation factor IID (TF... 28 4.5
At1g17440.1 68414.m02132 transcription initiation factor IID (TF... 28 4.5
At4g16700.1 68417.m02523 phosphatidylserine decarboxylase simila... 28 6.0
At2g45430.1 68415.m05651 DNA-binding protein-related contains Pf... 28 6.0
At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH) domain... 27 7.9
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 27 7.9
At3g20540.1 68416.m02600 DNA-directed DNA polymerase family prot... 27 7.9
At2g42580.1 68415.m05269 tetratricopeptide repeat (TPR)-containi... 27 7.9
>At3g49290.1 68416.m05387 expressed protein
Length = 312
Score = 34.3 bits (75), Expect = 0.069
Identities = 18/53 (33%), Positives = 30/53 (56%)
Frame = +3
Query: 312 QFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDTRKR 470
QF N A+ + + PPPP+ +ST+ +++ PPQ + T + ST + KR
Sbjct: 165 QFRN-AVRATIRETPPPPVRKSTSQSSSPRQPPQRSATFSFTSTIPKKEQDKR 216
>At4g17950.1 68417.m02673 DNA-binding family protein contains Pfam
PF03479: Domain of unknown function (DUF296); contains
Pfam PF02178: AT hook motif;
Length = 439
Score = 32.7 bits (71), Expect = 0.21
Identities = 19/62 (30%), Positives = 28/62 (45%)
Frame = +3
Query: 288 KSQHQRNVQFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQSVDTRK 467
+ Q Q+ Q + QQPPP ++ S N+ N P + GH+TS S +
Sbjct: 11 QQQQQQQQQQQQQQQHLQQQQQPPPGMLMSHHNSYNRN-PNAAAAVLMGHNTSTSQAMHQ 69
Query: 468 RL 473
RL
Sbjct: 70 RL 71
>At1g23420.1 68414.m02934 inner no outer protein (INO) identical to
INNER NO OUTER (INO) [Arabidopsis thaliana] GI:6684816
Length = 231
Score = 32.7 bits (71), Expect = 0.21
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = +2
Query: 296 APEKRSVSEQCTNNGDGSAT-PSTSCEEHGQHNNYERASASDRHDEW 433
A KR+ S+QC D +A P E+H + NN R + RH W
Sbjct: 179 AHNKRAASDQCFCEEDNNAILPCNVFEDHEESNNGFRERKAQRHSIW 225
>At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 575
Score = 29.9 bits (64), Expect = 1.5
Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 4/91 (4%)
Frame = +3
Query: 282 PHKSQHQRNVQFPNSALTMAMGQQPP---PPLVRSTANTTTMNVPPQVTGTMNGHSTSQS 452
P K HQ +VQ P + + +QP P S+ NTTT++ PP MN S+
Sbjct: 173 PLKHHHQVSVQ-PITTNPTFLAKQPSSTTPFPFYSSNNTTTVSQPPISNDLMNNVSSLNL 231
Query: 453 VDTRKRLTPIPDINTTGNQ-RRIRYKRR*WK 542
+ + D +Q + R KR+ WK
Sbjct: 232 FSSSTSSSTASDEEEDHHQVKSSRKKRKYWK 262
>At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 498
Score = 29.9 bits (64), Expect = 1.5
Identities = 27/91 (29%), Positives = 40/91 (43%), Gaps = 4/91 (4%)
Frame = +3
Query: 282 PHKSQHQRNVQFPNSALTMAMGQQPP---PPLVRSTANTTTMNVPPQVTGTMNGHSTSQS 452
P K HQ +VQ P + + +QP P S+ NTTT++ PP MN S+
Sbjct: 96 PLKHHHQVSVQ-PITTNPTFLAKQPSSTTPFPFYSSNNTTTVSQPPISNDLMNNVSSLNL 154
Query: 453 VDTRKRLTPIPDINTTGNQ-RRIRYKRR*WK 542
+ + D +Q + R KR+ WK
Sbjct: 155 FSSSTSSSTASDEEEDHHQVKSSRKKRKYWK 185
>At5g64540.1 68418.m08111 hypothetical protein
Length = 440
Score = 29.5 bits (63), Expect = 2.0
Identities = 14/41 (34%), Positives = 24/41 (58%)
Frame = +3
Query: 378 TANTTTMNVPPQVTGTMNGHSTSQSVDTRKRLTPIPDINTT 500
T +TT+ V + T + HS+S SVD + IPD++++
Sbjct: 305 TPISTTLTVDLNTSTTTSTHSSSPSVDRLDTVAQIPDLSSS 345
>At5g45300.1 68418.m05561 glycosyl hydrolase family 14 protein
similar to beta-amylase GI:13560977 from [Castanea
crenata]
Length = 689
Score = 29.1 bits (62), Expect = 2.6
Identities = 15/42 (35%), Positives = 19/42 (45%)
Frame = +3
Query: 114 RRTIGCLRSSARQQLVSTRNSYSGANAASAQVCSGSSGGAGG 239
RR G ++A + T N + N A GSSGG GG
Sbjct: 39 RRPRGFAAAAAAASIAPTENDVNNGNIAGIGGGEGSSGGGGG 80
>At3g18810.1 68416.m02389 protein kinase family protein contains
Pfam PF00069: Protein kinase domain
Length = 700
Score = 29.1 bits (62), Expect = 2.6
Identities = 20/60 (33%), Positives = 29/60 (48%), Gaps = 2/60 (3%)
Frame = +3
Query: 279 FPHKSQHQRNV--QFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQVTGTMNGHSTSQS 452
+ ++S H N+ Q+P S PPPP S + PP V+G MNG+S+ S
Sbjct: 240 YNNQSDHVMNLSQQYPGSNGNNNWMNSPPPPPPGSW-QPSPPPPPPPVSGGMNGNSSDFS 298
>At5g11460.1 68418.m01338 senescence-associated protein-related
similar to senescence-associated protein SAG102
(GI:22331931) [Arabidopsis thaliana]
Length = 344
Score = 28.3 bits (60), Expect = 4.5
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +2
Query: 383 QHNNYERASASDRHDEWPLNLSIRGH 460
QH+NY+ +ASD + P+ +IR H
Sbjct: 3 QHSNYQMTTASDYYSTKPVLSAIRSH 28
>At1g56530.1 68414.m06501 hydroxyproline-rich glycoprotein family
protein contains proline-rich extensin domains,
INTERPRO:IPR002965;
Length = 185
Score = 28.3 bits (60), Expect = 4.5
Identities = 19/55 (34%), Positives = 25/55 (45%), Gaps = 2/55 (3%)
Frame = +3
Query: 330 LTMAMGQQPPPPLVRSTANTTT-MNVPPQVTGTMNGHSTS-QSVDTRKRLTPIPD 488
LT A GQ PPPP TTT + PQ N + D R++ P+P+
Sbjct: 41 LTTAAGQPPPPPANHLRRRTTTAVAGQPQPPSPENRRRRNHHHNDHRRQPPPLPE 95
>At1g17440.2 68414.m02133 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +3
Query: 276 GFPHKSQHQRNVQFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQV 416
G +SQ + + PN + +GQ P++ A MN+ PQ+
Sbjct: 213 GSVQQSQQNQQMVNPNMSRAGLVGQSGHLPMLNGAAGAAQMNIQPQL 259
>At1g17440.1 68414.m02132 transcription initiation factor IID
(TFIID) subunit A family protein similar to SP|Q16514
Transcription initiation factor TFIID 20/15 kDa subunits
(TAFII-20/TAFII-15) {Homo sapiens}; contains Pfam
profile PF03847: Transcription initiation factor TFIID
subunit A
Length = 683
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/47 (27%), Positives = 22/47 (46%)
Frame = +3
Query: 276 GFPHKSQHQRNVQFPNSALTMAMGQQPPPPLVRSTANTTTMNVPPQV 416
G +SQ + + PN + +GQ P++ A MN+ PQ+
Sbjct: 213 GSVQQSQQNQQMVNPNMSRAGLVGQSGHLPMLNGAAGAAQMNIQPQL 259
>At4g16700.1 68417.m02523 phosphatidylserine decarboxylase similar
to SP|P27465 Phosphatidylserine decarboxylase proenzyme
(EC 4.1.1.65 {Cricetulus griseus}; contains Pfam profile
PF02666: phosphatidylserine decarboxylase
Length = 453
Score = 27.9 bits (59), Expect = 6.0
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +3
Query: 156 LVSTRNSYSGANAASAQVCSGSSGGAGG 239
L + R++YSGA A+ SG+ GAGG
Sbjct: 33 LNNIRSNYSGARASPLGGSSGAGAGAGG 60
>At2g45430.1 68415.m05651 DNA-binding protein-related contains Pfam
domain PF03479: Domain of unknown function (DUF296),
found in AT-hook motifs Pfam:PF02178
Length = 317
Score = 27.9 bits (59), Expect = 6.0
Identities = 16/58 (27%), Positives = 25/58 (43%)
Frame = +3
Query: 66 QHTEAHSYRCHHADVPRRTIGCLRSSARQQLVSTRNSYSGANAASAQVCSGSSGGAGG 239
QH + + H D+ + IG L+ + +S +G + S G SGG GG
Sbjct: 26 QHQQQQQQQNHGHDIDQHRIGGLKRDRDADIDPNEHSSAGKD-QSTPGSGGESGGGGG 82
>At5g11710.1 68418.m01368 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related contains Pfam PF01417: ENTH domain. ENTH
(Epsin N-terminal homology) domain; similar to
Af10-protein (GI:1724114) [Avena fatua]; similar to
clathrin assembly protein AP180 (GI:6492344) [Xenopus
laevis]; Af10-protein, Avena fatua, EMBL:U80041
Length = 560
Score = 27.5 bits (58), Expect = 7.9
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = +3
Query: 138 SSARQQLVSTRNSYSGANAASAQVCSGSSGGAGG 239
S R + V+ RN Y G ++ SGSS GG
Sbjct: 149 SEIRDKAVANRNKYVGLSSTGITYKSGSSASFGG 182
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 27.5 bits (58), Expect = 7.9
Identities = 11/19 (57%), Positives = 12/19 (63%)
Frame = +3
Query: 354 PPPPLVRSTANTTTMNVPP 410
PPPP TA TT N+PP
Sbjct: 236 PPPPPPPVTAKATTTNLPP 254
>At3g20540.1 68416.m02600 DNA-directed DNA polymerase family protein
similar to PolI-like DNA polymerase [Oryza sativa]
GI:19912795; contains Pfam profiles PF01612: 3'-5'
exonuclease, PF00476: DNA polymerase I family A
Length = 1034
Score = 27.5 bits (58), Expect = 7.9
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 2/38 (5%)
Frame = +2
Query: 338 GDGSATPSTSCE-EHG-QHNNYERASASDRHDEWPLNL 445
G+ + +T C HG H+ ++R+S+ + EW LNL
Sbjct: 46 GNAGYSTATDCGGSHGFHHSGHQRSSSVEFSGEWKLNL 83
>At2g42580.1 68415.m05269 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515 TPR Domain
Length = 691
Score = 27.5 bits (58), Expect = 7.9
Identities = 18/55 (32%), Positives = 24/55 (43%)
Frame = +3
Query: 168 RNSYSGANAASAQVCSGSSGGAGGRPVQHXXXXXXXGFPHKSQHQRNVQFPNSAL 332
R S S + AA+ SGSSG A G+P +KS H + P S +
Sbjct: 50 RGSASSSAAATPTSSSGSSGSASGKPSVSSQMAKRLDDAYKS-HSGELSSPGSGM 103
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,197,172
Number of Sequences: 28952
Number of extensions: 333086
Number of successful extensions: 1247
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1140
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1241
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1314848736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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