BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00250 (505 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 80 2e-16 SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 79 3e-16 SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 1.6 SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 26 2.8 SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosa... 26 3.7 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 25 4.9 SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 4.9 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 25 4.9 SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccha... 25 6.5 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 25 6.5 SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|ch... 25 8.5 SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch... 25 8.5 SPBC2G2.08 |ade9||C-1-tetrahydrofolatesynthase/methylenetetrahyd... 25 8.5 >SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 3|||Manual Length = 145 Score = 80.2 bits (189), Expect = 2e-16 Identities = 37/58 (63%), Positives = 45/58 (77%) Frame = +3 Query: 78 MDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLAENCDEAAYKKLVQALC 251 + V AL+EVLK AL+H GL G+ EA KALD+RQA LCVL E+CD+ AY KLV+ALC Sbjct: 27 VSVEDALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALC 84 Score = 77.0 bits (181), Expect = 2e-15 Identities = 31/54 (57%), Positives = 42/54 (77%) Frame = +2 Query: 254 QHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 415 + + PL+KV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 86 ESETPLIKVADPKVLGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSAALQKL 139 >SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 79.4 bits (187), Expect = 3e-16 Identities = 36/58 (62%), Positives = 45/58 (77%) Frame = +3 Query: 78 MDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLAENCDEAAYKKLVQALC 251 + V +L+EVLK AL+H GL G+ EA KALD+RQA LCVL E+CD+ AY KLV+ALC Sbjct: 30 LSVEDSLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALC 87 Score = 78.6 bits (185), Expect = 5e-16 Identities = 33/54 (61%), Positives = 42/54 (77%) Frame = +2 Query: 254 QHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVL 415 + Q PLVKV + K LGEWAGLC +D+DG ARK+VGCSCV + D+GE++ AL L Sbjct: 89 ESQTPLVKVADPKILGEWAGLCVLDRDGNARKVVGCSCVAVTDYGEDSVALQTL 142 >SPBC106.13 |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 404 Score = 27.1 bits (57), Expect = 1.6 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = -1 Query: 403 RWSFLAEIFDDNTGAADNFPCLAIL 329 RW +LA +F N A N P + +L Sbjct: 257 RWEYLASLFTSNFTAVHNIPSVPLL 281 >SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual Length = 111 Score = 26.2 bits (55), Expect = 2.8 Identities = 11/22 (50%), Positives = 14/22 (63%) Frame = -3 Query: 197 NT*YCLPLVKSFIGFVKTVHKT 132 N YCLP +FI FVK + +T Sbjct: 90 NRLYCLPFCNAFIEFVKKLTET 111 >SPAC17G8.03c |dpb3||DNA polymerase epsilon subunit Dpb3|Schizosaccharomyces pombe|chr 1|||Manual Length = 199 Score = 25.8 bits (54), Expect = 3.7 Identities = 13/55 (23%), Positives = 25/55 (45%) Frame = +3 Query: 153 EADKALDKRQAVLCVLAENCDEAAYKKLVQALCNNIRFHWSRLTTTKSLENGLVS 317 +AD+ + K V V+ E + ++Q C R H ++ T L++ + S Sbjct: 34 QADQDVGKVAQVTPVIMSKALELFMQSIIQESCKQTRLHQAKRVTVSHLKHAVQS 88 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 25.4 bits (53), Expect = 4.9 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -2 Query: 228 CMRLHHSSQPKHIVLLASCQELYRLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 64 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ubp21|Schizosaccharomyces pombe|chr 2|||Manual Length = 1129 Score = 25.4 bits (53), Expect = 4.9 Identities = 14/38 (36%), Positives = 20/38 (52%) Frame = +3 Query: 39 VEVPTNPILSGNNMDVNVALQEVLKTALIHGGLVHGLH 152 +E+ P LS D + + VL L+HGG +HG H Sbjct: 445 LEIDLEPYLS-ETADKSESHVYVLHGVLVHGGDLHGGH 481 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 25.4 bits (53), Expect = 4.9 Identities = 15/55 (27%), Positives = 24/55 (43%) Frame = -2 Query: 228 CMRLHHSSQPKHIVLLASCQELYRLREDRAQDHHGLKLSLAPLAVLHSRPCCYPT 64 C+ S +HIV A L +D +DH G+ L ++P V + P+ Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVVSYRESVSEPS 571 >SPAPJ696.01c |vps17||retromer complex subunit Vps17|Schizosaccharomyces pombe|chr 1|||Manual Length = 549 Score = 25.0 bits (52), Expect = 6.5 Identities = 10/28 (35%), Positives = 20/28 (71%), Gaps = 1/28 (3%) Frame = -3 Query: 371 QHRSSRQFSLPCHPCQSCRD-QPILQAF 291 + ++ +QF LP PCQ+ + +PI+++F Sbjct: 226 KRKALKQFPLPPDPCQALANLRPIVKSF 253 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 25.0 bits (52), Expect = 6.5 Identities = 11/32 (34%), Positives = 15/32 (46%) Frame = +3 Query: 213 DEAAYKKLVQALCNNIRFHWSRLTTTKSLENG 308 DE V L + ++ W R TT S E+G Sbjct: 14 DEPTTSSRVSMLVDRVKLLWKRYTTPLSTEDG 45 >SPCC576.17c |||membrane transporter|Schizosaccharomyces pombe|chr 3|||Manual Length = 525 Score = 24.6 bits (51), Expect = 8.5 Identities = 11/40 (27%), Positives = 18/40 (45%) Frame = -2 Query: 168 ELYRLREDRAQDHHGLKLSLAPLAVLHSRPCCYPTRWGWW 49 + Y L E+ + KL+ + + P +P WGWW Sbjct: 44 DTYELEENEDISDYAYKLA----GISNEHPA-HPQNWGWW 78 >SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1133 Score = 24.6 bits (51), Expect = 8.5 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -2 Query: 135 DHHGLKLSLAPLAVLHSRPC 76 D GL ++ L+++HSRPC Sbjct: 529 DEMGLGKTIEVLSLIHSRPC 548 >SPBC2G2.08 |ade9||C-1- tetrahydrofolatesynthase/methylenetetrahydrofolatedehydr ogenase/methylenetetrahydrofolatecyclohydrolase/formylte trahydrofolatesynthetase|Schizosaccharomyces pombe|chr 2|||Manual Length = 969 Score = 24.6 bits (51), Expect = 8.5 Identities = 11/20 (55%), Positives = 15/20 (75%) Frame = +3 Query: 345 GKLSAAPVLSSKISARKLQR 404 G S+A +L+ K+ ARKLQR Sbjct: 28 GTSSSANILNGKLLARKLQR 47 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,989,483 Number of Sequences: 5004 Number of extensions: 38103 Number of successful extensions: 138 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 138 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 200198394 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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