Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00250
(505 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32060.3 68415.m03918 40S ribosomal protein S12 (RPS12C) 89 2e-18
At2g32060.2 68415.m03917 40S ribosomal protein S12 (RPS12C) 89 2e-18
At2g32060.1 68415.m03916 40S ribosomal protein S12 (RPS12C) 89 2e-18
At1g15930.2 68414.m01912 40S ribosomal protein S12 (RPS12A) simi... 85 2e-17
At1g15930.1 68414.m01911 40S ribosomal protein S12 (RPS12A) simi... 85 2e-17
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 32 0.19
At5g45640.1 68418.m05612 subtilase family protein contains Pfam ... 28 3.1
At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein si... 27 5.4
At5g05930.2 68418.m00655 guanylyl cyclase-related (GC1) similar ... 27 5.4
At5g05930.1 68418.m00656 guanylyl cyclase-related (GC1) similar ... 27 5.4
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 27 9.5
At2g04740.1 68415.m00484 ankyrin repeat family protein contains ... 27 9.5
At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containi... 27 9.5
At1g78700.1 68414.m09173 brassinosteroid signalling positive reg... 27 9.5
>At2g32060.3 68415.m03918 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 89.0 bits (211), Expect = 2e-18
Identities = 38/57 (66%), Positives = 47/57 (82%)
Frame = +2
Query: 257 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 427
H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 85 HSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 68.9 bits (161), Expect = 2e-12
Identities = 30/59 (50%), Positives = 45/59 (76%)
Frame = +3
Query: 75 NMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLAENCDEAAYKKLVQALC 251
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+ALC
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALC 82
>At2g32060.2 68415.m03917 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 89.0 bits (211), Expect = 2e-18
Identities = 38/57 (66%), Positives = 47/57 (82%)
Frame = +2
Query: 257 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 427
H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 85 HSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 68.9 bits (161), Expect = 2e-12
Identities = 30/59 (50%), Positives = 45/59 (76%)
Frame = +3
Query: 75 NMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLAENCDEAAYKKLVQALC 251
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+ALC
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALC 82
>At2g32060.1 68415.m03916 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 89.0 bits (211), Expect = 2e-18
Identities = 38/57 (66%), Positives = 47/57 (82%)
Frame = +2
Query: 257 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 427
H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 85 HSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 68.9 bits (161), Expect = 2e-12
Identities = 30/59 (50%), Positives = 45/59 (76%)
Frame = +3
Query: 75 NMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLAENCDEAAYKKLVQALC 251
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+ALC
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALC 82
>At1g15930.2 68414.m01912 40S ribosomal protein S12 (RPS12A) similar
to 40S ribosomal protein S12 GI:4263712 from
[Arabidopsis thaliana]
Length = 144
Score = 85.4 bits (202), Expect = 2e-17
Identities = 34/57 (59%), Positives = 46/57 (80%)
Frame = +2
Query: 257 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 427
H++ L+ V + K LGEWAGLCKID +G ARK+VGCSC+V+KDFGEET AL ++ ++
Sbjct: 85 HEVRLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVVKDFGEETTALSIVNKHI 141
Score = 66.1 bits (154), Expect = 1e-11
Identities = 40/91 (43%), Positives = 53/91 (58%), Gaps = 1/91 (1%)
Frame = +3
Query: 27 ADVEVEVPT-NPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLA 203
A V V P P +MD+ AL+ L+ A +GG+V GLHE K ++KR A L VLA
Sbjct: 7 APVVVPPPVAEPAAIPEDMDLMTALELTLRKARAYGGVVRGLHECAKLIEKRVAQLVVLA 66
Query: 204 ENCDEAAYKKLVQALCNNIRFHWSRLTTTKS 296
E+C++ Y KLV+ALC + H RL T S
Sbjct: 67 EDCNQPDYVKLVKALCAD---HEVRLLTVPS 94
>At1g15930.1 68414.m01911 40S ribosomal protein S12 (RPS12A) similar
to 40S ribosomal protein S12 GI:4263712 from
[Arabidopsis thaliana]
Length = 144
Score = 85.4 bits (202), Expect = 2e-17
Identities = 34/57 (59%), Positives = 46/57 (80%)
Frame = +2
Query: 257 HQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 427
H++ L+ V + K LGEWAGLCKID +G ARK+VGCSC+V+KDFGEET AL ++ ++
Sbjct: 85 HEVRLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVVKDFGEETTALSIVNKHI 141
Score = 66.1 bits (154), Expect = 1e-11
Identities = 40/91 (43%), Positives = 53/91 (58%), Gaps = 1/91 (1%)
Frame = +3
Query: 27 ADVEVEVPT-NPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLCVLA 203
A V V P P +MD+ AL+ L+ A +GG+V GLHE K ++KR A L VLA
Sbjct: 7 APVVVPPPVAEPAAIPEDMDLMTALELTLRKARAYGGVVRGLHECAKLIEKRVAQLVVLA 66
Query: 204 ENCDEAAYKKLVQALCNNIRFHWSRLTTTKS 296
E+C++ Y KLV+ALC + H RL T S
Sbjct: 67 EDCNQPDYVKLVKALCAD---HEVRLLTVPS 94
>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|O04630
Threonyl-tRNA synthetase, mitochondrial precursor (EC
6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
thaliana}; contains Pfam profiles PF00587: tRNA
synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain, PF02824: TGS domain
Length = 458
Score = 32.3 bits (70), Expect = 0.19
Identities = 17/52 (32%), Positives = 29/52 (55%)
Frame = +3
Query: 168 LDKRQAVLCVLAENCDEAAYKKLVQALCNNIRFHWSRLTTTKSLENGLVSAR 323
L RQA++C L+E+C ++Y K VQ N + ++ + +SL + AR
Sbjct: 355 LSPRQAIVCSLSEDC--SSYAKQVQKQINEVGYYVDIDESDRSLRKKVADAR 404
>At5g45640.1 68418.m05612 subtilase family protein contains Pfam
domain, PF00082: Subtilase family; contains Pfam domain,
PF02225: protease associated (PA) domain
Length = 754
Score = 28.3 bits (60), Expect = 3.1
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -2
Query: 192 IVLLASCQELYRLREDRAQDHHGLKLSLAP 103
+ LLASC + +LRE+RA +G L P
Sbjct: 18 VPLLASCTKEKQLREERASSINGFAAELTP 47
>At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein
similar to beta-glucan-elicitor receptor GI:1752734 from
[Glycine max]
Length = 745
Score = 27.5 bits (58), Expect = 5.4
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +1
Query: 391 GNSSVGCAQGLPQVVELILRCNANPY 468
G SS+ C G ++ +++ N NPY
Sbjct: 233 GVSSINCGDGFSGIIRIVVLPNPNPY 258
>At5g05930.2 68418.m00655 guanylyl cyclase-related (GC1) similar to
guanylyl cyclase (GC1) GI:21436486 from [Arabidopsis
thaliana]; identical to cDNA putative guanylate cyclase
GI:21436486
Length = 210
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/45 (28%), Positives = 23/45 (51%)
Frame = +3
Query: 60 ILSGNNMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLC 194
+LSGN + + + Q+ L + + LV GLH ++ V+C
Sbjct: 111 LLSGNYIAIALVDQDKLSKSWLEEVLVSGLHSSNSCYTGHYVVIC 155
>At5g05930.1 68418.m00656 guanylyl cyclase-related (GC1) similar to
guanylyl cyclase (GC1) GI:21436486 from [Arabidopsis
thaliana]; identical to cDNA putative guanylate cyclase
GI:21436486
Length = 274
Score = 27.5 bits (58), Expect = 5.4
Identities = 13/45 (28%), Positives = 23/45 (51%)
Frame = +3
Query: 60 ILSGNNMDVNVALQEVLKTALIHGGLVHGLHEADKALDKRQAVLC 194
+LSGN + + + Q+ L + + LV GLH ++ V+C
Sbjct: 175 LLSGNYIAIALVDQDKLSKSWLEEVLVSGLHSSNSCYTGHYVVIC 219
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 26.6 bits (56), Expect = 9.5
Identities = 11/20 (55%), Positives = 16/20 (80%)
Frame = -1
Query: 103 SCSATFTSMLLPDKMGLVGT 44
SC+ TF S LPD++G++GT
Sbjct: 423 SCAGTFVS--LPDELGMLGT 440
>At2g04740.1 68415.m00484 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 578
Score = 26.6 bits (56), Expect = 9.5
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2
Query: 228 CMRLHHSSQPKHIVLLASCQELYRLREDRAQDHH 127
C+R+ + H V+LAS E +R R R D H
Sbjct: 341 CVRVDKRNFYCHQVILASRSEYFRARLSRVNDFH 374
>At2g02980.1 68415.m00250 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 603
Score = 26.6 bits (56), Expect = 9.5
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 287 NKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEE 394
NKK L K+ KD KA K+ GCS + + + E
Sbjct: 446 NKKWEYVDSLRKVMKDRKAVKVPGCSSIEVNNVVHE 481
>At1g78700.1 68414.m09173 brassinosteroid signalling positive
regulator-related contains similarity to BZR1 protein
[Arabidopsis thaliana] gi|20270971|gb|AAM18490
Length = 325
Score = 26.6 bits (56), Expect = 9.5
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = -1
Query: 130 PWIKAVFSTSCSATFTSMLLPDKMGLVGTSTS 35
PW+K + +TS S+ +S LP+ + + G S S
Sbjct: 134 PWLKHLSTTSSSSASSSSRLPNYLYIPGGSIS 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,730,907
Number of Sequences: 28952
Number of extensions: 209848
Number of successful extensions: 672
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 656
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 672
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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