SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00245
         (750 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo...    31   0.23 
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy...    30   0.31 
SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom...    30   0.40 
SPBC215.03c |csn1||COP9/signalosome complex subunit Csn1|Schizos...    28   1.2  
SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|...    27   2.2  
SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch...    27   3.8  
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb...    26   5.0  
SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S...    26   6.6  
SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo...    26   6.6  
SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ...    25   8.7  
SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces...    25   8.7  
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo...    25   8.7  

>SPCC1739.11c |cdc11||SIN component scaffold protein
           Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1045

 Score = 30.7 bits (66), Expect = 0.23
 Identities = 13/22 (59%), Positives = 16/22 (72%)
 Frame = +3

Query: 663 FSGLVYLSSLDVSYNDLDDLKG 728
           FS L+ L  LD+SYN L+DL G
Sbjct: 664 FSNLLNLQYLDISYNQLEDLTG 685



 Score = 28.3 bits (60), Expect = 1.2
 Identities = 17/62 (27%), Positives = 31/62 (50%)
 Frame = +1

Query: 31  HTGDMDTLDVSYNMLIEFNLNNTQISSLTKLLLNNNFIKTISDRTLLGLTSLKEIIVTHN 210
           H   +  L    N + E +  N+ +  L +LLL NN I+ I +  +  L +L  + + +N
Sbjct: 710 HLDGLLKLSACNNRIKELSFTNSNLHRLEELLLGNNEIEEIEE--ISSLQNLMVLQLDNN 767

Query: 211 KI 216
           K+
Sbjct: 768 KL 769



 Score = 25.4 bits (53), Expect = 8.7
 Identities = 8/18 (44%), Positives = 17/18 (94%)
 Frame = +2

Query: 515 AFSDLKSMRFLDLSYNKI 568
           +FS+L ++++LD+SYN++
Sbjct: 663 SFSNLLNLQYLDISYNQL 680


>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1010

 Score = 30.3 bits (65), Expect = 0.31
 Identities = 26/80 (32%), Positives = 36/80 (45%), Gaps = 2/80 (2%)
 Frame = +3

Query: 288 LAFNANIVKSSMYLNKLIISG--LEPNISLIGLGNSSVAILDISCSKITNVQKLHLNELK 461
           +  NAN  + S+ +N   I+      N S + L   S   LDI CSKI  VQ  +     
Sbjct: 361 MVVNANHKRQSIQVNHFDITSDACMLNFSHV-LSRLSEPFLDIGCSKIDRVQVEYFRRNP 419

Query: 462 RLDDLKLYKNNIIKLESERF 521
           R+D  +  K N  +  SE F
Sbjct: 420 RVDIKEETKLNADQKASESF 439


>SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 396

 Score = 29.9 bits (64), Expect = 0.40
 Identities = 19/47 (40%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
 Frame = +1

Query: 103 ISSLTKLLLNNNFIKTISDRTLLGLTSLKEI-IVTHNKIEHFSDNSL 240
           ISS+T +LLN   +KT+S R  +G+ SL  I  + ++K   FS  +L
Sbjct: 154 ISSVTNILLNIPTVKTLSIRWCVGVCSLSLIEFLQNSKSRTFSLENL 200


>SPBC215.03c |csn1||COP9/signalosome complex subunit
           Csn1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 422

 Score = 28.3 bits (60), Expect = 1.2
 Identities = 23/71 (32%), Positives = 39/71 (54%), Gaps = 1/71 (1%)
 Frame = +3

Query: 402 LDIS-CSKITNVQKLHLNELKRLDDLKLYKNNIIKLESERFRI*NL*DFSI*ATTKFLTS 578
           LD+     +T  +K +L +L+R  +LK YKNN+I+ ES R    +L  F      +F ++
Sbjct: 76  LDVEWVESVTFHRKQNLEQLRR--ELKAYKNNLIR-ESIRAAQLDLASFFA-DVGQFDSA 131

Query: 579 NLGYSRITPYC 611
              Y+++  YC
Sbjct: 132 LRSYAKVREYC 142


>SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 646

 Score = 27.5 bits (58), Expect = 2.2
 Identities = 11/33 (33%), Positives = 19/33 (57%)
 Frame = -2

Query: 179 VSPSKVLSDIVLIKLLFSNNFVKLEIWVLFKLN 81
           ++PS+   D V I LL + N   L++W++   N
Sbjct: 303 ITPSEQFLDKVFISLLSAKNSCSLKMWLIHSFN 335


>SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2176

 Score = 26.6 bits (56), Expect = 3.8
 Identities = 10/16 (62%), Positives = 12/16 (75%)
 Frame = -1

Query: 732  FHPSDRPNRCTIHLKN 685
            F P DRPN  TIHL++
Sbjct: 1552 FSPKDRPNPLTIHLQS 1567


>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 2685

 Score = 26.2 bits (55), Expect = 5.0
 Identities = 11/28 (39%), Positives = 17/28 (60%)
 Frame = -3

Query: 430  FVILEQEISRIATDEFPRPINDILGSKP 347
            F+I++ +   +   +FPR IN IL  KP
Sbjct: 1344 FMIVDSDKFYLTIPDFPRQINGILEGKP 1371


>SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein
           Pkl1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 832

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 12/20 (60%), Positives = 14/20 (70%)
 Frame = +3

Query: 450 NELKRLDDLKLYKNNIIKLE 509
           N LKR+ DLK    NII+LE
Sbjct: 133 NTLKRIKDLKSKPKNIIQLE 152


>SPAC1039.07c |||4-aminobutyrate aminotransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 448

 Score = 25.8 bits (54), Expect = 6.6
 Identities = 11/17 (64%), Positives = 12/17 (70%)
 Frame = -3

Query: 733 LSPFRSSKSLYDTSKEL 683
           LSPFR +K  YD  KEL
Sbjct: 181 LSPFRDAKGNYDWQKEL 197


>SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1429

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 15/49 (30%), Positives = 21/49 (42%)
 Frame = -1

Query: 552 KSRNLIDFKSENAPTPI*LYYFCTISNHLASLVRLNATSERLLFWNKKY 406
           ++ NLI  KSEN P P            L S +  +A S  ++ W   Y
Sbjct: 111 QNANLISSKSENVPEPAGEKVSMPEKQDLQSALPSDAVSNAVIGWKSIY 159


>SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 879

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%)
 Frame = +3

Query: 273 SFNDKLAFNANIVKSSMYL-NKLIISGLEPNISLIGLGNSSVAILDISCSKITNVQKL 443
           S   K + N +    ++ L N +++ G +P     GLGN     LDI+ + I N+ KL
Sbjct: 248 SMGSKTSGNTSATFRTVLLANNVVLLGNKP-----GLGNVGGGALDITDADIRNINKL 300


>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
            Wis4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1401

 Score = 25.4 bits (53), Expect = 8.7
 Identities = 16/58 (27%), Positives = 30/58 (51%)
 Frame = +3

Query: 429  NVQKLHLNELKRLDDLKLYKNNIIKLESERFRI*NL*DFSI*ATTKFLTSNLGYSRIT 602
            ++Q L    + R+D+L++ +NN +    ER  I ++ D S+     F+     +S IT
Sbjct: 980  SIQLLQKETMLRIDELEIERNNTL---LERLAIGHVLDDSVFRNRDFIKLASSFSNIT 1034


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,067,031
Number of Sequences: 5004
Number of extensions: 62281
Number of successful extensions: 187
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 357280532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -