BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00245 (750 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 31 0.23 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 30 0.31 SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pom... 30 0.40 SPBC215.03c |csn1||COP9/signalosome complex subunit Csn1|Schizos... 28 1.2 SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 27 2.2 SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosacch... 27 3.8 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.0 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 26 6.6 SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharo... 26 6.6 SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ... 25 8.7 SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces... 25 8.7 SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 25 8.7 >SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizosaccharomyces pombe|chr 3|||Manual Length = 1045 Score = 30.7 bits (66), Expect = 0.23 Identities = 13/22 (59%), Positives = 16/22 (72%) Frame = +3 Query: 663 FSGLVYLSSLDVSYNDLDDLKG 728 FS L+ L LD+SYN L+DL G Sbjct: 664 FSNLLNLQYLDISYNQLEDLTG 685 Score = 28.3 bits (60), Expect = 1.2 Identities = 17/62 (27%), Positives = 31/62 (50%) Frame = +1 Query: 31 HTGDMDTLDVSYNMLIEFNLNNTQISSLTKLLLNNNFIKTISDRTLLGLTSLKEIIVTHN 210 H + L N + E + N+ + L +LLL NN I+ I + + L +L + + +N Sbjct: 710 HLDGLLKLSACNNRIKELSFTNSNLHRLEELLLGNNEIEEIEE--ISSLQNLMVLQLDNN 767 Query: 211 KI 216 K+ Sbjct: 768 KL 769 Score = 25.4 bits (53), Expect = 8.7 Identities = 8/18 (44%), Positives = 17/18 (94%) Frame = +2 Query: 515 AFSDLKSMRFLDLSYNKI 568 +FS+L ++++LD+SYN++ Sbjct: 663 SFSNLLNLQYLDISYNQL 680 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 30.3 bits (65), Expect = 0.31 Identities = 26/80 (32%), Positives = 36/80 (45%), Gaps = 2/80 (2%) Frame = +3 Query: 288 LAFNANIVKSSMYLNKLIISG--LEPNISLIGLGNSSVAILDISCSKITNVQKLHLNELK 461 + NAN + S+ +N I+ N S + L S LDI CSKI VQ + Sbjct: 361 MVVNANHKRQSIQVNHFDITSDACMLNFSHV-LSRLSEPFLDIGCSKIDRVQVEYFRRNP 419 Query: 462 RLDDLKLYKNNIIKLESERF 521 R+D + K N + SE F Sbjct: 420 RVDIKEETKLNADQKASESF 439 >SPBC1271.01c |pof13||F-box protein Pof13|Schizosaccharomyces pombe|chr 2|||Manual Length = 396 Score = 29.9 bits (64), Expect = 0.40 Identities = 19/47 (40%), Positives = 29/47 (61%), Gaps = 1/47 (2%) Frame = +1 Query: 103 ISSLTKLLLNNNFIKTISDRTLLGLTSLKEI-IVTHNKIEHFSDNSL 240 ISS+T +LLN +KT+S R +G+ SL I + ++K FS +L Sbjct: 154 ISSVTNILLNIPTVKTLSIRWCVGVCSLSLIEFLQNSKSRTFSLENL 200 >SPBC215.03c |csn1||COP9/signalosome complex subunit Csn1|Schizosaccharomyces pombe|chr 2|||Manual Length = 422 Score = 28.3 bits (60), Expect = 1.2 Identities = 23/71 (32%), Positives = 39/71 (54%), Gaps = 1/71 (1%) Frame = +3 Query: 402 LDIS-CSKITNVQKLHLNELKRLDDLKLYKNNIIKLESERFRI*NL*DFSI*ATTKFLTS 578 LD+ +T +K +L +L+R +LK YKNN+I+ ES R +L F +F ++ Sbjct: 76 LDVEWVESVTFHRKQNLEQLRR--ELKAYKNNLIR-ESIRAAQLDLASFFA-DVGQFDSA 131 Query: 579 NLGYSRITPYC 611 Y+++ YC Sbjct: 132 LRSYAKVREYC 142 >SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 27.5 bits (58), Expect = 2.2 Identities = 11/33 (33%), Positives = 19/33 (57%) Frame = -2 Query: 179 VSPSKVLSDIVLIKLLFSNNFVKLEIWVLFKLN 81 ++PS+ D V I LL + N L++W++ N Sbjct: 303 ITPSEQFLDKVFISLLSAKNSCSLKMWLIHSFN 335 >SPAC9.03c |brr2|spp41|U5 snRNP complex subunit Brr2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2176 Score = 26.6 bits (56), Expect = 3.8 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = -1 Query: 732 FHPSDRPNRCTIHLKN 685 F P DRPN TIHL++ Sbjct: 1552 FSPKDRPNPLTIHLQS 1567 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 26.2 bits (55), Expect = 5.0 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = -3 Query: 430 FVILEQEISRIATDEFPRPINDILGSKP 347 F+I++ + + +FPR IN IL KP Sbjct: 1344 FMIVDSDKFYLTIPDFPRQINGILEGKP 1371 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 25.8 bits (54), Expect = 6.6 Identities = 12/20 (60%), Positives = 14/20 (70%) Frame = +3 Query: 450 NELKRLDDLKLYKNNIIKLE 509 N LKR+ DLK NII+LE Sbjct: 133 NTLKRIKDLKSKPKNIIQLE 152 >SPAC1039.07c |||4-aminobutyrate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 448 Score = 25.8 bits (54), Expect = 6.6 Identities = 11/17 (64%), Positives = 12/17 (70%) Frame = -3 Query: 733 LSPFRSSKSLYDTSKEL 683 LSPFR +K YD KEL Sbjct: 181 LSPFRDAKGNYDWQKEL 197 >SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1429 Score = 25.4 bits (53), Expect = 8.7 Identities = 15/49 (30%), Positives = 21/49 (42%) Frame = -1 Query: 552 KSRNLIDFKSENAPTPI*LYYFCTISNHLASLVRLNATSERLLFWNKKY 406 ++ NLI KSEN P P L S + +A S ++ W Y Sbjct: 111 QNANLISSKSENVPEPAGEKVSMPEKQDLQSALPSDAVSNAVIGWKSIY 159 >SPCC1682.02c |mcm3||MCM complex subunit Mcm3|Schizosaccharomyces pombe|chr 3|||Manual Length = 879 Score = 25.4 bits (53), Expect = 8.7 Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 1/58 (1%) Frame = +3 Query: 273 SFNDKLAFNANIVKSSMYL-NKLIISGLEPNISLIGLGNSSVAILDISCSKITNVQKL 443 S K + N + ++ L N +++ G +P GLGN LDI+ + I N+ KL Sbjct: 248 SMGSKTSGNTSATFRTVLLANNVVLLGNKP-----GLGNVGGGALDITDADIRNINKL 300 >SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1401 Score = 25.4 bits (53), Expect = 8.7 Identities = 16/58 (27%), Positives = 30/58 (51%) Frame = +3 Query: 429 NVQKLHLNELKRLDDLKLYKNNIIKLESERFRI*NL*DFSI*ATTKFLTSNLGYSRIT 602 ++Q L + R+D+L++ +NN + ER I ++ D S+ F+ +S IT Sbjct: 980 SIQLLQKETMLRIDELEIERNNTL---LERLAIGHVLDDSVFRNRDFIKLASSFSNIT 1034 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,067,031 Number of Sequences: 5004 Number of extensions: 62281 Number of successful extensions: 187 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 187 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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