Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00232
(523 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g32060.3 68415.m03918 40S ribosomal protein S12 (RPS12C) 93 1e-19
At2g32060.2 68415.m03917 40S ribosomal protein S12 (RPS12C) 93 1e-19
At2g32060.1 68415.m03916 40S ribosomal protein S12 (RPS12C) 93 1e-19
At1g15930.2 68414.m01912 40S ribosomal protein S12 (RPS12A) simi... 89 2e-18
At1g15930.1 68414.m01911 40S ribosomal protein S12 (RPS12A) simi... 89 2e-18
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 30 1.1
At5g45640.1 68418.m05612 subtilase family protein contains Pfam ... 29 2.5
At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein si... 27 5.8
>At2g32060.3 68415.m03918 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 92.7 bits (220), Expect = 1e-19
Identities = 39/60 (65%), Positives = 49/60 (81%)
Frame = +3
Query: 255 CNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 434
C +H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 82 CADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 66.5 bits (155), Expect = 1e-11
Identities = 33/74 (44%), Positives = 50/74 (67%)
Frame = +1
Query: 82 NMDVNVALQEVLKTALIHGGLVHGLHEAXKALDKRQAVLCVLAENCDEAAYKKLVQALAT 261
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+AL
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCA 83
Query: 262 NIRFHWSRLTTTKS 303
+ H +L T S
Sbjct: 84 D---HSIKLLTVPS 94
>At2g32060.2 68415.m03917 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 92.7 bits (220), Expect = 1e-19
Identities = 39/60 (65%), Positives = 49/60 (81%)
Frame = +3
Query: 255 CNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 434
C +H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 82 CADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 66.5 bits (155), Expect = 1e-11
Identities = 33/74 (44%), Positives = 50/74 (67%)
Frame = +1
Query: 82 NMDVNVALQEVLKTALIHGGLVHGLHEAXKALDKRQAVLCVLAENCDEAAYKKLVQALAT 261
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+AL
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCA 83
Query: 262 NIRFHWSRLTTTKS 303
+ H +L T S
Sbjct: 84 D---HSIKLLTVPS 94
>At2g32060.1 68415.m03916 40S ribosomal protein S12 (RPS12C)
Length = 144
Score = 92.7 bits (220), Expect = 1e-19
Identities = 39/60 (65%), Positives = 49/60 (81%)
Frame = +3
Query: 255 CNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 434
C +H I L+ V + K LGEWAGLCKID +G ARK+VGCSC+VIKDFGEET AL+++K +L
Sbjct: 82 CADHSIKLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVIKDFGEETTALNIVKKHL 141
Score = 66.5 bits (155), Expect = 1e-11
Identities = 33/74 (44%), Positives = 50/74 (67%)
Frame = +1
Query: 82 NMDVNVALQEVLKTALIHGGLVHGLHEAXKALDKRQAVLCVLAENCDEAAYKKLVQALAT 261
+MDV+ AL+ ++ + +GG+V GLHE+ K ++KR A LCVLAE+C++ Y KLV+AL
Sbjct: 24 DMDVSTALELTVRKSRAYGGVVRGLHESAKLIEKRNAQLCVLAEDCNQPDYVKLVKALCA 83
Query: 262 NIRFHWSRLTTTKS 303
+ H +L T S
Sbjct: 84 D---HSIKLLTVPS 94
>At1g15930.2 68414.m01912 40S ribosomal protein S12 (RPS12A) similar
to 40S ribosomal protein S12 GI:4263712 from
[Arabidopsis thaliana]
Length = 144
Score = 89.0 bits (211), Expect = 2e-18
Identities = 35/60 (58%), Positives = 48/60 (80%)
Frame = +3
Query: 255 CNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 434
C +H++ L+ V + K LGEWAGLCKID +G ARK+VGCSC+V+KDFGEET AL ++ ++
Sbjct: 82 CADHEVRLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVVKDFGEETTALSIVNKHI 141
Score = 63.7 bits (148), Expect = 7e-11
Identities = 39/91 (42%), Positives = 52/91 (57%), Gaps = 1/91 (1%)
Frame = +1
Query: 34 ADVEVEVPT-NPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEAXKALDKRQAVLCVLA 210
A V V P P +MD+ AL+ L+ A +GG+V GLHE K ++KR A L VLA
Sbjct: 7 APVVVPPPVAEPAAIPEDMDLMTALELTLRKARAYGGVVRGLHECAKLIEKRVAQLVVLA 66
Query: 211 ENCDEAAYKKLVQALATNIRFHWSRLTTTKS 303
E+C++ Y KLV+AL + H RL T S
Sbjct: 67 EDCNQPDYVKLVKALCAD---HEVRLLTVPS 94
>At1g15930.1 68414.m01911 40S ribosomal protein S12 (RPS12A) similar
to 40S ribosomal protein S12 GI:4263712 from
[Arabidopsis thaliana]
Length = 144
Score = 89.0 bits (211), Expect = 2e-18
Identities = 35/60 (58%), Positives = 48/60 (80%)
Frame = +3
Query: 255 CNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKIVGCSCVVIKDFGEETPALDVLKDYL 434
C +H++ L+ V + K LGEWAGLCKID +G ARK+VGCSC+V+KDFGEET AL ++ ++
Sbjct: 82 CADHEVRLLTVPSAKTLGEWAGLCKIDSEGNARKVVGCSCLVVKDFGEETTALSIVNKHI 141
Score = 63.7 bits (148), Expect = 7e-11
Identities = 39/91 (42%), Positives = 52/91 (57%), Gaps = 1/91 (1%)
Frame = +1
Query: 34 ADVEVEVPT-NPILSGNNMDVNVALQEVLKTALIHGGLVHGLHEAXKALDKRQAVLCVLA 210
A V V P P +MD+ AL+ L+ A +GG+V GLHE K ++KR A L VLA
Sbjct: 7 APVVVPPPVAEPAAIPEDMDLMTALELTLRKARAYGGVVRGLHECAKLIEKRVAQLVVLA 66
Query: 211 ENCDEAAYKKLVQALATNIRFHWSRLTTTKS 303
E+C++ Y KLV+AL + H RL T S
Sbjct: 67 EDCNQPDYVKLVKALCAD---HEVRLLTVPS 94
>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|O04630
Threonyl-tRNA synthetase, mitochondrial precursor (EC
6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
thaliana}; contains Pfam profiles PF00587: tRNA
synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain, PF02824: TGS domain
Length = 458
Score = 29.9 bits (64), Expect = 1.1
Identities = 16/52 (30%), Positives = 28/52 (53%)
Frame = +1
Query: 175 LDKRQAVLCVLAENCDEAAYKKLVQALATNIRFHWSRLTTTKSLENGLVSAR 330
L RQA++C L+E+C ++Y K VQ + ++ + +SL + AR
Sbjct: 355 LSPRQAIVCSLSEDC--SSYAKQVQKQINEVGYYVDIDESDRSLRKKVADAR 404
>At5g45640.1 68418.m05612 subtilase family protein contains Pfam
domain, PF00082: Subtilase family; contains Pfam domain,
PF02225: protease associated (PA) domain
Length = 754
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 199 IVLLASCQELXRLREDRAQDHHGLKLSLAP 110
+ LLASC + +LRE+RA +G L P
Sbjct: 18 VPLLASCTKEKQLREERASSINGFAAELTP 47
>At5g15870.1 68418.m01857 glycosyl hydrolase family 81 protein
similar to beta-glucan-elicitor receptor GI:1752734 from
[Glycine max]
Length = 745
Score = 27.5 bits (58), Expect = 5.8
Identities = 9/26 (34%), Positives = 15/26 (57%)
Frame = +2
Query: 398 GNSSVGCAQGLPQVVELILRCNANPY 475
G SS+ C G ++ +++ N NPY
Sbjct: 233 GVSSINCGDGFSGIIRIVVLPNPNPY 258
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,591,844
Number of Sequences: 28952
Number of extensions: 203562
Number of successful extensions: 629
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 617
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 629
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 957410176
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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