BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00227X (431 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 117 7e-28 SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 66 3e-12 SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 62 4e-11 SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 59 3e-10 SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 40 2e-04 SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 39 4e-04 SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 38 5e-04 SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|c... 27 1.6 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 26 2.2 SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosacc... 26 2.2 SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 26 2.8 SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual 25 3.8 SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 25 5.0 SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 25 6.6 SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosacc... 24 8.7 SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosa... 24 8.7 SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 24 8.7 SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 24 8.7 >SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2|||Manual Length = 375 Score = 117 bits (282), Expect = 7e-28 Identities = 52/59 (88%), Positives = 54/59 (91%) Frame = +2 Query: 254 NEAQSKRGILTLKYPIVHGIVTNWDDMEMIWHHTF*NELRVAPEEHPVLLTEAPLNPKA 430 +EAQSKRGILTLKYPI HGIV NWDDME IWHHTF NELRVAPEEHP LLTEAPLNPK+ Sbjct: 56 DEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKS 114 Score = 110 bits (265), Expect = 8e-26 Identities = 48/60 (80%), Positives = 56/60 (93%) Frame = +3 Query: 93 DEEVAALVVDHGSGMCKAGYAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGMRHRAK 272 +EE+AALV+D+GSGMCKAG+AGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVG ++K Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61 >SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 379 Score = 65.7 bits (153), Expect = 3e-12 Identities = 29/56 (51%), Positives = 40/56 (71%), Gaps = 1/56 (1%) Frame = +2 Query: 254 NEAQSKRGILTLKYPIVHGIVTNWDDMEMIWHHTF*N-ELRVAPEEHPVLLTEAPL 418 +EAQ+ RG+L ++ PI GI+ NW DME IW + + + +L PEEHP+LLTE PL Sbjct: 60 SEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPL 115 Score = 60.5 bits (140), Expect = 1e-10 Identities = 25/48 (52%), Positives = 35/48 (72%) Frame = +3 Query: 111 LVVDHGSGMCKAGYAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVG 254 + +D+GSG KAG+AGDD P+ +FP+ VGR +H+ VM QKD +VG Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVG 59 >SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 62.1 bits (144), Expect = 4e-11 Identities = 24/72 (33%), Positives = 47/72 (65%) Frame = +2 Query: 215 RDGRYGTEGLLCRNEAQSKRGILTLKYPIVHGIVTNWDDMEMIWHHTF*NELRVAPEEHP 394 + G + ++ +EA++ R +L +KYP+ +GI+ ++++M +W +TF +L++ P Sbjct: 43 KTGNVQIKDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRK 102 Query: 395 VLLTEAPLNPKA 430 +LLTE P+NP A Sbjct: 103 ILLTEPPMNPVA 114 Score = 47.6 bits (108), Expect = 8e-07 Identities = 21/35 (60%), Positives = 26/35 (74%) Frame = +3 Query: 99 EVAALVVDHGSGMCKAGYAGDDAPRAVFPSIVGRP 203 E A +V+D+G+G K GYA D+ PR FPSIVGRP Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36 >SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces pombe|chr 1|||Manual Length = 427 Score = 58.8 bits (136), Expect = 3e-10 Identities = 28/58 (48%), Positives = 33/58 (56%), Gaps = 1/58 (1%) Frame = +2 Query: 254 NEAQSKRGI-LTLKYPIVHGIVTNWDDMEMIWHHTF*NELRVAPEEHPVLLTEAPLNP 424 N+A K +L YPI HG + NWD ME W + LR PE+H LLTE PLNP Sbjct: 78 NDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNP 135 Score = 42.3 bits (95), Expect = 3e-05 Identities = 20/52 (38%), Positives = 32/52 (61%), Gaps = 2/52 (3%) Frame = +3 Query: 111 LVVDHGSGMCKAGYAGDDAPRAVFPSIVGRPRHQGVMVG--MGQKDSYVGMR 260 +++D+G+G K GYAG+DAP VFP+++ R G G + K SY+ + Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIA-TRSAGASSGPAVSSKPSYMASK 58 >SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 39.9 bits (89), Expect = 2e-04 Identities = 18/48 (37%), Positives = 27/48 (56%) Frame = +2 Query: 281 LTLKYPIVHGIVTNWDDMEMIWHHTF*NELRVAPEEHPVLLTEAPLNP 424 + +K I +G V NWD +W + +L+ P EHP+L+TE NP Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNP 110 Score = 39.1 bits (87), Expect = 3e-04 Identities = 15/34 (44%), Positives = 24/34 (70%) Frame = +3 Query: 96 EEVAALVVDHGSGMCKAGYAGDDAPRAVFPSIVG 197 +EV+A+V+D GS + G++G+D P+ V PS G Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42 >SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 721 Score = 38.7 bits (86), Expect = 4e-04 Identities = 21/71 (29%), Positives = 36/71 (50%), Gaps = 1/71 (1%) Frame = +2 Query: 215 RDGRYGTEGLLCRNEAQSKRGILTL-KYPIVHGIVTNWDDMEMIWHHTF*NELRVAPEEH 391 RD + L N+ + G ++ + P +++NWD ME + +TF +L + EH Sbjct: 58 RDRKLSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTF-LKLGIDRMEH 116 Query: 392 PVLLTEAPLNP 424 P+ +TE NP Sbjct: 117 PICMTEPLANP 127 Score = 34.3 bits (75), Expect = 0.008 Identities = 14/32 (43%), Positives = 22/32 (68%) Frame = +3 Query: 111 LVVDHGSGMCKAGYAGDDAPRAVFPSIVGRPR 206 LV+D+GS +AG+ G+ P+ VF ++V R R Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58 >SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Schizosaccharomyces pombe|chr 1|||Manual Length = 430 Score = 38.3 bits (85), Expect = 5e-04 Identities = 15/34 (44%), Positives = 23/34 (67%) Frame = +3 Query: 96 EEVAALVVDHGSGMCKAGYAGDDAPRAVFPSIVG 197 EE+ +LV+D GS + GYAG+++P + PS G Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41 Score = 33.1 bits (72), Expect = 0.019 Identities = 12/42 (28%), Positives = 24/42 (57%) Frame = +2 Query: 305 HGIVTNWDDMEMIWHHTF*NELRVAPEEHPVLLTEAPLNPKA 430 +GI+ +W+ W +L+V P E+ +++TE NP++ Sbjct: 74 NGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQS 115 >SPAC23C4.03 |||haspin related kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 488 Score = 26.6 bits (56), Expect = 1.6 Identities = 13/45 (28%), Positives = 23/45 (51%) Frame = -1 Query: 263 VPHSYIRVLLSHTDHHALMAGPSHDRGEHGARSIISCVTGLAHTG 129 V +Y +LL D + + G +DR + + + + CV L H+G Sbjct: 223 VKGTYPSLLLEEWDRYLMQNGSENDRPDSYSSTQLYCVLCLDHSG 267 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 26.2 bits (55), Expect = 2.2 Identities = 8/25 (32%), Positives = 14/25 (56%) Frame = -2 Query: 376 DTQLILEGMMPNHLHVIPVSDDSVY 302 D Q ++ NH H +P+ D ++Y Sbjct: 376 DMQTFFSDLVSNHQHYVPIIDAAIY 400 >SPBC19C2.11c |||mitochondrial outer membrane protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 452 Score = 26.2 bits (55), Expect = 2.2 Identities = 13/42 (30%), Positives = 23/42 (54%) Frame = -3 Query: 339 ISMSSQLVTIPCTMGYLRVRIPLLLCASFLHKSPSVPYRPSR 214 + +SS +IP +L+ I + L + F + PS+ Y+ SR Sbjct: 155 VRVSSSFDSIPAIARFLQREIEVQLVSLFQEELPSIIYKMSR 196 >SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizosaccharomyces pombe|chr 1|||Manual Length = 927 Score = 25.8 bits (54), Expect = 2.8 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = -3 Query: 252 LHKSPSVPYRPSRPDGGAFPRSRGTR 175 + K+PS PY SRP A S TR Sbjct: 310 ISKAPSYPYSSSRPSASASLASSPTR 335 >SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1828 Score = 25.4 bits (53), Expect = 3.8 Identities = 10/17 (58%), Positives = 11/17 (64%) Frame = -3 Query: 138 TYRSHDLLPTRQLLRRT 88 TYR HD +PT LL T Sbjct: 168 TYRDHDAIPTATLLNPT 184 >SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 25.0 bits (52), Expect = 5.0 Identities = 13/31 (41%), Positives = 16/31 (51%) Frame = -2 Query: 232 PIPTITP*WRGLPTIEGNTARGASSPA*PAL 140 P+PT P LPT NT + P+ PAL Sbjct: 74 PVPTGAP---SLPTSSNNTQQAEERPSMPAL 101 >SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr 1|||Manual Length = 1517 Score = 24.6 bits (51), Expect = 6.6 Identities = 9/25 (36%), Positives = 15/25 (60%) Frame = +3 Query: 156 GDDAPRAVFPSIVGRPRHQGVMVGM 230 G+ PRA F ++ P H G+++ M Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM 526 >SPCP1E11.04c |pal1||membrane associated protein Pal1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 425 Score = 24.2 bits (50), Expect = 8.7 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -3 Query: 150 NRPCTYRSHDLLPTRQ 103 NRP YRSH + TR+ Sbjct: 323 NRPSNYRSHSSMGTRR 338 >SPBC2G2.02 |syj1||inositol-polyphosphate 5-phosphatase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1076 Score = 24.2 bits (50), Expect = 8.7 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 263 VPHSYIRVLLSHTDHHALMA 204 VPHSY V L ++DH + A Sbjct: 825 VPHSYQSVPLYYSDHRPIYA 844 >SPAC7D4.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 551 Score = 24.2 bits (50), Expect = 8.7 Identities = 9/17 (52%), Positives = 12/17 (70%) Frame = +3 Query: 375 SPPRNTQSCSLRLPSTP 425 SPP++T+ S R PS P Sbjct: 285 SPPQSTRPSSTRFPSVP 301 >SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosaccharomyces pombe|chr 1|||Manual Length = 523 Score = 24.2 bits (50), Expect = 8.7 Identities = 7/21 (33%), Positives = 12/21 (57%) Frame = +2 Query: 296 PIVHGIVTNWDDMEMIWHHTF 358 PI G V +W+ ++ W H + Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,877,553 Number of Sequences: 5004 Number of extensions: 40507 Number of successful extensions: 108 Number of sequences better than 10.0: 18 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 106 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 154067960 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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