Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00223
(839 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino... 157 9e-39
At4g38220.2 68417.m05395 aminoacylase, putative / N-acyl-L-amino... 155 3e-38
At4g38220.1 68417.m05394 aminoacylase, putative / N-acyl-L-amino... 155 3e-38
At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino... 148 4e-36
At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein si... 43 2e-04
At2g27800.1 68415.m03370 pentatricopeptide (PPR) repeat-containi... 31 1.3
At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase, puta... 30 1.7
At4g36550.1 68417.m05190 U-box domain-containing protein low sim... 30 2.2
At1g56570.1 68414.m06506 pentatricopeptide (PPR) repeat-containi... 29 5.1
At5g27120.1 68418.m03237 SAR DNA-binding protein, putative stron... 28 6.7
At1g43190.1 68414.m04977 polypyrimidine tract-binding protein, p... 28 8.9
At1g16420.1 68414.m01964 latex-abundant protein, putative (AMC8)... 28 8.9
>At1g44820.1 68414.m05134 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 438
Score = 157 bits (381), Expect = 9e-39
Identities = 70/148 (47%), Positives = 99/148 (66%), Gaps = 1/148 (0%)
Frame = +2
Query: 68 TSSVKIDPSVSTLQNYIRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVM 247
+SS + D ++ Q Y+R + HPN +Y I+FL N+A+ IGL + +E + KP L++
Sbjct: 21 SSSSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIGLTTKTIEFISGKPILLI 80
Query: 248 TWVGEQPELPSILLNSHMDVVPVFENSWKHPPFAA-EIEDNVIYARGVQDMKSVGIQYIE 424
TW+G P LPSIL NSH+D VP W +PPF+A + D IYARG QD K +G+QY+E
Sbjct: 81 TWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHIYARGAQDDKCIGVQYLE 140
Query: 425 AVRRLKETGVKLKRTVHLSFVPDEEIGG 508
++R LK G RT+H+S+VP+EEIGG
Sbjct: 141 SIRNLKSRGFSPLRTIHISYVPEEEIGG 168
Score = 68.1 bits (159), Expect = 7e-12
Identities = 34/92 (36%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
Frame = +1
Query: 514 GMGKFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPD 693
GM KF + FK++N+GFA+DEG A+P D++ VF +R+ WH I G GHG+ L +
Sbjct: 171 GMMKFAASSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDN 230
Query: 694 NCGEKLR---YIIDKFMDLRQESVKKWLTTHS 780
+ E L +I +F + + + VK +S
Sbjct: 231 SAMENLMKSVELISRFRESQFDFVKAGKAAYS 262
>At4g38220.2 68417.m05395 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 433
Score = 155 bits (376), Expect = 3e-38
Identities = 72/158 (45%), Positives = 108/158 (68%), Gaps = 1/158 (0%)
Frame = +2
Query: 38 LISAMLRIHTTSSVKIDPSVSTLQNYIRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVE 217
L+ ++ +H ++ D VS Q Y+RI +V PN +Y + ++F+ ++A+ + L+ Q +E
Sbjct: 6 LLLVVVVLHLSAVAGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIE 65
Query: 218 PLPKKPTLVMTWVGEQPELPSILLNSHMDVVPVFENSWKHPPFAAEIEDN-VIYARGVQD 394
+ KP L++ WVG P LP+ LLNSH DVVP ++ W H P A ++ + IYARG QD
Sbjct: 66 FVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQD 125
Query: 395 MKSVGIQYIEAVRRLKETGVKLKRTVHLSFVPDEEIGG 508
MK VG+QY+EA+R+L+ +G K R+V+LSFVPDEEIGG
Sbjct: 126 MKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGG 163
Score = 64.9 bits (151), Expect = 6e-11
Identities = 30/79 (37%), Positives = 43/79 (54%)
Frame = +1
Query: 508 HTGMGKFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLL 687
H G KF ++ +FK++N+ LDEG+ SP + Y VF GER W + I G GHG+ L
Sbjct: 164 HDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLY 223
Query: 688 PDNCGEKLRYIIDKFMDLR 744
++ E L I+ R
Sbjct: 224 DNSAMENLLKSIESIRRFR 242
>At4g38220.1 68417.m05394 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1)[Homo sapiens] SWISS-PROT:Q03154
Length = 430
Score = 155 bits (376), Expect = 3e-38
Identities = 72/158 (45%), Positives = 108/158 (68%), Gaps = 1/158 (0%)
Frame = +2
Query: 38 LISAMLRIHTTSSVKIDPSVSTLQNYIRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVE 217
L+ ++ +H ++ D VS Q Y+RI +V PN +Y + ++F+ ++A+ + L+ Q +E
Sbjct: 6 LLLVVVVLHLSAVAGDDAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIE 65
Query: 218 PLPKKPTLVMTWVGEQPELPSILLNSHMDVVPVFENSWKHPPFAAEIEDN-VIYARGVQD 394
+ KP L++ WVG P LP+ LLNSH DVVP ++ W H P A ++ + IYARG QD
Sbjct: 66 FVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQD 125
Query: 395 MKSVGIQYIEAVRRLKETGVKLKRTVHLSFVPDEEIGG 508
MK VG+QY+EA+R+L+ +G K R+V+LSFVPDEEIGG
Sbjct: 126 MKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGG 163
Score = 64.9 bits (151), Expect = 6e-11
Identities = 30/79 (37%), Positives = 43/79 (54%)
Frame = +1
Query: 508 HTGMGKFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLL 687
H G KF ++ +FK++N+ LDEG+ SP + Y VF GER W + I G GHG+ L
Sbjct: 164 HDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLY 223
Query: 688 PDNCGEKLRYIIDKFMDLR 744
++ E L I+ R
Sbjct: 224 DNSAMENLLKSIESIRRFR 242
>At1g44180.1 68414.m05103 aminoacylase, putative /
N-acyl-L-amino-acid amidohydrolase, putative similar to
aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
ACY-1) [Homo sapiens] SWISS-PROT:Q03154
Length = 435
Score = 148 bits (359), Expect = 4e-36
Identities = 67/142 (47%), Positives = 91/142 (64%), Gaps = 1/142 (0%)
Frame = +2
Query: 86 DPSVSTLQNYIRIRSVHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVMTWVGEQ 265
D ++ Q Y+R + HPN +Y I+FL ++A IGL + +E +P KP L++TW+G
Sbjct: 29 DTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIGLTSRTIEFVPGKPVLLLTWLGSN 88
Query: 266 PELPSILLNSHMDVVPVFENSWKHPPFAA-EIEDNVIYARGVQDMKSVGIQYIEAVRRLK 442
L SIL NSH+D VP W HPPF+A D IYARG QD K +G+QY+EA+R LK
Sbjct: 89 LNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHIYARGAQDDKCIGVQYLEAIRNLK 148
Query: 443 ETGVKLKRTVHLSFVPDEEIGG 508
RT+H+S+VP+EEIGG
Sbjct: 149 SRSFSPLRTIHISYVPEEEIGG 170
Score = 68.5 bits (160), Expect = 5e-12
Identities = 31/78 (39%), Positives = 45/78 (57%)
Frame = +1
Query: 514 GMGKFVQTDVFKNMNVGFALDEGVASPNDDYLVFNGERIIWHVKITCPGKSGHGSLLLPD 693
GM KF + FK++N+GF +DEG ASP D++ VF ER WH+ I G GHG+ L +
Sbjct: 173 GMMKFAASSEFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDN 232
Query: 694 NCGEKLRYIIDKFMDLRQ 747
+ E L ++ R+
Sbjct: 233 SAMENLMKSVELISRFRE 250
>At4g17830.1 68417.m02659 peptidase M20/M25/M40 family protein
similar to acetylornithine deacetylase
(Acetylornithinase, AO; N-acetylornithinase, NAO)
[Dictyostelium discoideum] SWISS-PROT:P54638
Length = 440
Score = 43.2 bits (97), Expect = 2e-04
Identities = 25/91 (27%), Positives = 40/91 (43%)
Frame = +2
Query: 239 LVMTWVGEQPELPSILLNSHMDVVPVFENSWKHPPFAAEIEDNVIYARGVQDMKSVGIQY 418
L++ + G P + HMDVV + W+ PF+ I+ + + RG D
Sbjct: 82 LIVEYPGSVPGKILSFVGMHMDVVTANPDDWEFDPFSLSIDGDKLRGRGTTDCLGHVALV 141
Query: 419 IEAVRRLKETGVKLKRTVHLSFVPDEEIGGI 511
E +++L + LK TV F+ EE I
Sbjct: 142 TELMKKLGQAKPALKSTVVAVFIASEENSSI 172
>At2g27800.1 68415.m03370 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 427
Score = 30.7 bits (66), Expect = 1.3
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -2
Query: 397 HILNSSSVNYIVFYFSSKGRMLPAVLEDRHHV 302
HI N + N I+FYF+ G+++ AV RH V
Sbjct: 188 HIGNENLYNSIIFYFTKAGKLIRAVNIFRHMV 219
>At5g43600.1 68418.m05330 N-carbamyl-L-amino acid hydrolase,
putative similar to N-carbamoyl-L-amino acid hydrolase
[Bacillus stearothermophilus] SWISS-PROT:Q53389
Length = 476
Score = 30.3 bits (65), Expect = 1.7
Identities = 25/83 (30%), Positives = 37/83 (44%)
Frame = +2
Query: 251 WVGEQPELPSILLNSHMDVVPVFENSWKHPPFAAEIEDNVIYARGVQDMKSVGIQYIEAV 430
W G +P LP++ SH+D +P S K+ D V+ G IEA+
Sbjct: 123 WDGLEPNLPAVATGSHIDAIPY---SGKY--------DGVVGVLGA----------IEAI 161
Query: 431 RRLKETGVKLKRTVHLSFVPDEE 499
LK +G K KR++ + EE
Sbjct: 162 NVLKRSGFKPKRSLEIILFTSEE 184
>At4g36550.1 68417.m05190 U-box domain-containing protein low
similarity to immediate-early fungal elicitor protein
CMPG1 [Petroselinum crispum] GI:14582200; contains Pfam
profile PF04564: U-box domain
Length = 577
Score = 29.9 bits (64), Expect = 2.2
Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 2/61 (3%)
Frame = -3
Query: 201 CSPIFSASFLRKLMHSL*STFGCTERILI*--FCKVETLGSILTELVVCILSIAEIRQNG 28
C + S F++KL L C I+I C E +TE C+ SIAE+ ++
Sbjct: 396 CLEMVSLDFIQKLTSFLQQKVFCKHSIIILKNLCSTEKGRGCITETPDCLASIAELLESN 455
Query: 27 V 25
V
Sbjct: 456 V 456
>At1g56570.1 68414.m06506 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 611
Score = 28.7 bits (61), Expect = 5.1
Identities = 15/41 (36%), Positives = 22/41 (53%)
Frame = +2
Query: 131 VHPNVDYNECINFLKNEAEKIGLQVQVVEPLPKKPTLVMTW 253
++P+ D C+ L A KIG ++VE +P KP TW
Sbjct: 447 INPDRDIYNCVVDLLGRAGKIGEAYELVERMPFKPD-ESTW 486
>At5g27120.1 68418.m03237 SAR DNA-binding protein, putative strong
similarity to SAR DNA-binding protein-1 [Pisum sativum]
GI:3132696; contains Pfam profile PF01798: Putative
snoRNA binding domain
Length = 533
Score = 28.3 bits (60), Expect = 6.7
Identities = 17/57 (29%), Positives = 28/57 (49%)
Frame = +2
Query: 227 KKPTLVMTWVGEQPELPSILLNSHMDVVPVFENSWKHPPFAAEIEDNVIYARGVQDM 397
K T+++ +G +L L M V F W P A ++DN++YA+ V+ M
Sbjct: 155 KVDTMIIQAIGLLDDLDKELNTYAMRVREWF--GWHFPELAKIVQDNILYAKAVKLM 209
>At1g43190.1 68414.m04977 polypyrimidine tract-binding protein,
putative / heterogeneous nuclear ribonucleoprotein,
putative similar to Polypyrimidine tract-binding protein
1 (PTB) (Heterogeneous nuclear ribonucleoprotein I)
(hnRNP I) from {Rattus norvegicus} SP|Q00438, {Homo
sapiens} SP|P26599, [Homo sapiens] GI:35770; contains
InterPro entry IPR000504: RNA-binding region RNP-1 (RNA
recognition motif) (RRM)
Length = 432
Score = 27.9 bits (59), Expect = 8.9
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = -3
Query: 510 MPPISSSGTNDRCTVLLS 457
+PP +GTNDRCTVL+S
Sbjct: 234 LPP-GITGTNDRCTVLVS 250
>At1g16420.1 68414.m01964 latex-abundant protein, putative (AMC8) /
caspase family protein similar to latex-abundant protein
[Hevea brasiliensis] gb:AAD13216; contains Pfam profile
PF00656: ICE-like protease (caspase) p20 domain
Length = 381
Score = 27.9 bits (59), Expect = 8.9
Identities = 14/49 (28%), Positives = 26/49 (53%)
Frame = -2
Query: 460 ELDTGFLQPPHGLNVLNSNRLHILNSSSVNYIVFYFSSKGRMLPAVLED 314
+ D +QP G N+ + I + S +++VF++S G +P +ED
Sbjct: 49 DTDKSCIQPT-GKNICDELDNLIASGQSGDFLVFHYSGHGTRIPPGIED 96
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,765,637
Number of Sequences: 28952
Number of extensions: 416016
Number of successful extensions: 1156
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1152
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1941125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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