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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00221
         (765 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g35930.1 68414.m04462 replication protein-related weak simila...    31   1.1  
At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative ...    30   1.5  
At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p...    30   1.9  
At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5) M...    28   5.9  
At3g17560.1 68416.m02242 F-box family protein contains F-box dom...    28   5.9  
At4g39640.2 68417.m05603 gamma-glutamyltranspeptidase family pro...    28   7.8  
At4g39640.1 68417.m05602 gamma-glutamyltranspeptidase family pro...    28   7.8  

>At1g35930.1 68414.m04462 replication protein-related weak
           similarity to Replication Protein A 70  kDa DNA-binding
           subunit (RP-A) (RF-A) (Replication factor-A protein 1)
           (Single-stranded DNA-binding protein) (DMRPA1)
           (GI:2498844)(SP:Q24492) [Drosophila melanogaster]
          Length = 261

 Score = 30.7 bits (66), Expect = 1.1
 Identities = 14/49 (28%), Positives = 24/49 (48%)
 Frame = -1

Query: 687 IVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEPF*DFLEFASSDVRMLCL 541
           +V  V  W+   +D   +   EF C R+  +P   + +FAS   +ML +
Sbjct: 211 VVCAVRFWRIASVDGENVLKSEFGCSRLHIDPSFPYFDFASGVSKMLMI 259


>At4g11130.1 68417.m01805 RNA-dependent RNA polymerase, putative
           similar to RNA-directed RNA polymerase [Lycopersicon
           esculentum] gi|4038592|emb|CAA71421
          Length = 1133

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 14/53 (26%), Positives = 26/53 (49%), Gaps = 5/53 (9%)
 Frame = +3

Query: 297 FWFWQSCHCLSLKIFLKRLISTM-----GSPRRLEIFPLNYCITPRIWSRVKV 440
           FW W+S  C  +++  + +I T+     G    ++ F L     P+++ RV V
Sbjct: 145 FWVWESGDCYKIEVRFEDIIETLSCCVNGDASEIDAFLLKLKYGPKVFKRVTV 197


>At1g11160.1 68414.m01278 WD-40 repeat family protein / katanin p80
            subunit, putative similar to contains 6 WD-40 repeats
            (PF00400); katanin p80 subunit (GI:3005601)
            [Strongylocentrotus purpuratus]
          Length = 974

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 25/111 (22%), Positives = 48/111 (43%)
 Frame = +2

Query: 326  FAQNIPKATNLHNGLTEKIGNFSIELLYHTSNLEQSKGNLIMSPITVWTVLAVIAEGASG 505
            F+Q +P  T+L    TE+  N S+++L     L    G +I S ++   ++ V       
Sbjct: 870  FSQLVPVLTSLLGSRTERPVNVSLDMLL---KLVAVFGTVIRSTVSAPRIVGV---DLHA 923

Query: 506  NRDGRSIMRYGYKQSIRTSLEANSKKSQNGSESTRTQ*NSPKSMPLLSISN 658
            N      +  G  Q  + S  +  K+  N  + +RT+ +S +++      N
Sbjct: 924  NERNLPNLFCGTAQDSKNSSSSRKKRRSNNKKGSRTKPSSSRTIAASKTKN 974


>At5g51710.1 68418.m06413 K+ efflux antiporter, putative (KEA5)
           Monovalent cation:proton antiporter family 2 (CPA2
           family) member, PMID:11500563; related to
           glutathione-regulated potassium-efflux system protein
           [Escherichia coli] GP|606284|gb|AAA58147
          Length = 568

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 17/60 (28%), Positives = 32/60 (53%), Gaps = 1/60 (1%)
 Frame = +2

Query: 275 LIMCLLKFLVLAIVPLSFAQNIPKATNLHNG-LTEKIGNFSIELLYHTSNLEQSKGNLIM 451
           +++ ++K  + A+V  +F  N+    + H G L  +IG F+  LL   SNL   +G + +
Sbjct: 453 ILVIVIKTAIAAVVVKAFRYNM--RISFHVGVLLAQIGEFAFVLLSRASNLHVIEGKMYL 510


>At3g17560.1 68416.m02242 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 413

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 14/41 (34%), Positives = 22/41 (53%)
 Frame = -1

Query: 714 HISFVIGLCIVMKVLLWQTLLIDNNGIDFGEFYCVRVDSEP 592
           H+S +   C  +KV +W T  ID   + + +F  + VD EP
Sbjct: 307 HLSLLYQSCETLKVEIWMTKEIDTTFVSWKKF--LTVDLEP 345


>At4g39640.2 68417.m05603 gamma-glutamyltranspeptidase family
           protein similar to SP|P19440
           Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2)
           (Gamma-glutamyltransferase 1) (CD224 antigen) {Homo
           sapiens}; contains Pfam profilePF01019:
           Gamma-glutamyltranspeptidase
          Length = 572

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 20/71 (28%), Positives = 33/71 (46%)
 Frame = +2

Query: 311 IVPLSFAQNIPKATNLHNGLTEKIGNFSIELLYHTSNLEQSKGNLIMSPITVWTVLAVIA 490
           + P  + Q IP   +  N  T  + N   E+   T  + + KG+ ++SPI   T+   I 
Sbjct: 488 LAPRIYHQLIPNRASYENWTT--VFNDHFEIPKATRVVLEKKGH-VLSPIAGGTIAQFIV 544

Query: 491 EGASGNRDGRS 523
           + +  N  GRS
Sbjct: 545 QESGENSGGRS 555


>At4g39640.1 68417.m05602 gamma-glutamyltranspeptidase family
           protein similar to SP|P19440
           Gamma-glutamyltranspeptidase 1 precursor (EC 2.3.2.2)
           (Gamma-glutamyltransferase 1) (CD224 antigen) {Homo
           sapiens}; contains Pfam profilePF01019:
           Gamma-glutamyltranspeptidase
          Length = 572

 Score = 27.9 bits (59), Expect = 7.8
 Identities = 20/71 (28%), Positives = 33/71 (46%)
 Frame = +2

Query: 311 IVPLSFAQNIPKATNLHNGLTEKIGNFSIELLYHTSNLEQSKGNLIMSPITVWTVLAVIA 490
           + P  + Q IP   +  N  T  + N   E+   T  + + KG+ ++SPI   T+   I 
Sbjct: 488 LAPRIYHQLIPNRASYENWTT--VFNDHFEIPKATRVVLEKKGH-VLSPIAGGTIAQFIV 544

Query: 491 EGASGNRDGRS 523
           + +  N  GRS
Sbjct: 545 QESGENSGGRS 555


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,813,219
Number of Sequences: 28952
Number of extensions: 321207
Number of successful extensions: 776
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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