Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00215X
(408 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD) 157 4e-39
At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB) si... 157 4e-39
At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA) si... 157 4e-39
At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC) ri... 155 8e-39
At1g78630.1 68414.m09164 ribosomal protein L13 family protein si... 37 0.005
At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR... 31 0.39
At3g15590.1 68416.m01975 DNA-binding protein, putative similar t... 29 1.2
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 29 1.6
At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing, put... 29 1.6
At5g04130.2 68418.m00400 DNA topoisomerase, ATP-hydrolyzing, put... 27 6.4
At5g04130.1 68418.m00399 DNA topoisomerase, ATP-hydrolyzing, put... 27 6.4
At3g46420.1 68416.m05032 leucine-rich repeat family protein / pr... 27 6.4
At3g15000.1 68416.m01897 expressed protein similar to DAG protei... 27 6.4
>At5g48760.1 68418.m06034 60S ribosomal protein L13A (RPL13aD)
Length = 206
Score = 157 bits (380), Expect = 4e-39
Identities = 71/122 (58%), Positives = 88/122 (72%)
Frame = +3
Query: 42 VVKMTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKLKLM 221
+V +G +K +V+D R H+LGRLA++ AK LL G KVV+V CE+I +SG R K+K M
Sbjct: 1 MVSGSGICSKRVVVDARHHMLGRLASITAKELLNGQKVVIVRCEEICLSGGLVRQKMKYM 60
Query: 222 SFLRKRCNVNPTRGPFHFRAPSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPPLDNR 401
FLRKR N P+ GP HFRAPSKI W+TVRGMIPHKT+RG AL RL+ YEG P P D
Sbjct: 61 RFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVYEGVPTPYDKI 120
Query: 402 RR 407
+R
Sbjct: 121 KR 122
>At3g24830.1 68416.m03115 60S ribosomal protein L13A (RPL13aB)
similar to 60S RIBOSOMAL PROTEIN L13A GB:P35427 from
[Rattus norvegicus]
Length = 206
Score = 157 bits (380), Expect = 4e-39
Identities = 71/122 (58%), Positives = 88/122 (72%)
Frame = +3
Query: 42 VVKMTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKLKLM 221
+V +G +K +V+D R H+ GRLA++IAK LL G VVVV CE+I +SG R K+K M
Sbjct: 1 MVSGSGICSKRVVVDARHHMCGRLASIIAKELLNGQSVVVVRCEEICLSGGLVRQKMKYM 60
Query: 222 SFLRKRCNVNPTRGPFHFRAPSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPPLDNR 401
FLRKR N P+ GP HFRAPSKI W+TVRGMIPHKT+RG AL RL+ +EG PPP D
Sbjct: 61 RFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVFEGVPPPYDKV 120
Query: 402 RR 407
+R
Sbjct: 121 KR 122
>At3g07110.1 68416.m00847 60S ribosomal protein L13A (RPL13aA)
similar to ribosomal protein L13A GB:O49885 [Lupinus
luteus]
Length = 206
Score = 157 bits (380), Expect = 4e-39
Identities = 71/122 (58%), Positives = 88/122 (72%)
Frame = +3
Query: 42 VVKMTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKLKLM 221
+V +G K +V+D R H+LGRLA+V+AK LL G +VVV CE+I +SG R K+K M
Sbjct: 1 MVSGSGICAKRVVVDARHHMLGRLASVVAKDLLNGQNIVVVRCEEICLSGGLVRQKMKYM 60
Query: 222 SFLRKRCNVNPTRGPFHFRAPSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPPLDNR 401
FLRKR N P+ GP HFRAPSKI W+TVRGMIPHKT+RG NAL RL+ +EG P P D
Sbjct: 61 RFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGANALARLKVFEGVPTPYDKI 120
Query: 402 RR 407
+R
Sbjct: 121 KR 122
>At4g13170.1 68417.m02049 60S ribosomal protein L13A (RPL13aC)
ribosomal protein L13a -Lupinus luteus,PID:e1237871
Length = 206
Score = 155 bits (377), Expect = 8e-39
Identities = 72/122 (59%), Positives = 88/122 (72%)
Frame = +3
Query: 42 VVKMTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKLKLM 221
+V +G K +V+DGR H+LGRLA+ AK LL G +VVVV CE+I +SG R K+K M
Sbjct: 1 MVSGSGICAKRVVVDGRHHMLGRLASNTAKELLNGQEVVVVRCEEICLSGGLVRQKMKYM 60
Query: 222 SFLRKRCNVNPTRGPFHFRAPSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPPLDNR 401
FLRKR N P+ GP HFRAPSKI W+TVRGMIPHKT+RG AL RL+ +EG PPP D
Sbjct: 61 RFLRKRMNTKPSHGPIHFRAPSKIFWRTVRGMIPHKTKRGAAALARLKVFEGIPPPYDKI 120
Query: 402 RR 407
+R
Sbjct: 121 KR 122
>At1g78630.1 68414.m09164 ribosomal protein L13 family protein
similar to ribosomal protein L13 GI:170132 from
[Spinacia oleracea]
Length = 241
Score = 37.1 bits (82), Expect = 0.005
Identities = 36/116 (31%), Positives = 49/116 (42%), Gaps = 12/116 (10%)
Frame = +3
Query: 78 VIDGRGHLLGRLAAVIA-----KVLLE-------GNKVVVVPCEQINISGNFFRNKLKLM 221
V+D +LGRLA+ IA K L G V+VV E++ +SG KL
Sbjct: 107 VVDATDKILGRLASTIANHIRGKNLASYTPSVDMGAFVIVVNAEKVAVSGKKRNQKLYRR 166
Query: 222 SFLRKRCNVNPTRGPFHFRAPSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPP 389
R T R P +I+ VRGM+P K G+ L+ Y+G P
Sbjct: 167 HSGRPGGMTVETFDQLQQRIPERIVEHAVRGMLP-KGRLGRALFNHLKVYKGPDHP 221
>At5g11250.1 68418.m01314 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1189
Score = 30.7 bits (66), Expect = 0.39
Identities = 18/46 (39%), Positives = 29/46 (63%)
Frame = -3
Query: 319 IIPLTVFHRILDGALKWKGPRVGFTLHLLRRNDISLSLFLKKLPEM 182
+I L + H LD + W+G + LH LR+ D+S S+ LK+LP++
Sbjct: 655 LIELNLTHSKLD--MLWEGVK---PLHNLRQMDLSYSVNLKELPDL 695
>At3g15590.1 68416.m01975 DNA-binding protein, putative similar to
DNA-binding protein [Triticum aestivum] GI:6958202;
contains Pfam profile: PF01535 PPR repeat
Length = 610
Score = 29.1 bits (62), Expect = 1.2
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +3
Query: 159 VVPCEQINISGNFF-RNKLKLMSFLRKRCNVNPTRGPFHFRAPSKILWKTVRGM 317
V C Q+ + + R K+ + L +R N+ P+R +HF SK L + GM
Sbjct: 277 VFACNQLLLLYSMHDRKKISDVLLLMERENIKPSRATYHFLINSKGLAGDITGM 330
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.7 bits (61), Expect = 1.6
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -2
Query: 161 HNNFVPFEKDLGDYCRQ 111
HN F F DL DYC Q
Sbjct: 414 HNGFASFNNDLDDYCNQ 430
>At3g10270.1 68416.m01231 DNA topoisomerase, ATP-hydrolyzing,
putative / DNA topoisomerase II, putative / DNA gyrase,
putative similar to SP|O50627 DNA gyrase subunit B (EC
5.99.1.3) {Bacillus halodurans}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00204: DNA topoisomerase II
(N-terminal region), PF00986: DNA gyrase, B subunit,
carboxyl terminus, PF01751: Toprim domain
Length = 657
Score = 28.7 bits (61), Expect = 1.6
Identities = 16/54 (29%), Positives = 27/54 (50%)
Frame = +3
Query: 51 MTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKL 212
M G++N IDG H+ G A++ + K+ V+ + I++SG R L
Sbjct: 286 MLGYANSIRTIDGGTHIEGVKASLTRTLNSLAKKLKVIKEKDISLSGEHVREGL 339
>At5g04130.2 68418.m00400 DNA topoisomerase, ATP-hydrolyzing,
putative / DNA topoisomerase II, putative / DNA gyrase,
putative similar to SP|O50627 DNA gyrase subunit B (EC
5.99.1.3) {Bacillus halodurans}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00204: DNA topoisomerase II
(N-terminal region), PF00986: DNA gyrase, B subunit,
carboxyl terminus, PF01751: Toprim domain
Length = 519
Score = 26.6 bits (56), Expect = 6.4
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = +3
Query: 51 MTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKL 212
M G++N IDG H+ G A++ + K V + I++SG R L
Sbjct: 361 MLGYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGL 414
>At5g04130.1 68418.m00399 DNA topoisomerase, ATP-hydrolyzing,
putative / DNA topoisomerase II, putative / DNA gyrase,
putative similar to SP|O50627 DNA gyrase subunit B (EC
5.99.1.3) {Bacillus halodurans}; contains Pfam profiles
PF02518: ATPase, histidine kinase-, DNA gyrase B-, and
HSP90-like domain protein, PF00204: DNA topoisomerase II
(N-terminal region), PF00986: DNA gyrase, B subunit,
carboxyl terminus, PF01751: Toprim domain
Length = 732
Score = 26.6 bits (56), Expect = 6.4
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = +3
Query: 51 MTGFSNKAIVIDGRGHLLGRLAAVIAKVLLEGNKVVVVPCEQINISGNFFRNKL 212
M G++N IDG H+ G A++ + K V + I++SG R L
Sbjct: 361 MLGYANSIRTIDGGTHIEGVKASLTRTLNTLAKKSKTVKEKDISLSGEHVREGL 414
>At3g46420.1 68416.m05032 leucine-rich repeat family protein /
protein kinase family protein contains leucine rich
repeat (LRR) domains, INTERPRO:IPR001611; contains
serine/threonine protein kinases active-site signature,
Prosite:PS00108
Length = 838
Score = 26.6 bits (56), Expect = 6.4
Identities = 14/40 (35%), Positives = 20/40 (50%)
Frame = +3
Query: 279 APSKILWKTVRGMIPHKTERGKNALRRLRTYEGGPPPLDN 398
+P + KT+R P ERG+ L ++T PPL N
Sbjct: 308 SPVYLQTKTIRNPSPVTCERGECILEMIKTERSTLPPLLN 347
>At3g15000.1 68416.m01897 expressed protein similar to DAG protein
(required for chloroplast differentiation and palisade
development) GB:Q38732 [Antirrhinum majus]
Length = 395
Score = 26.6 bits (56), Expect = 6.4
Identities = 10/23 (43%), Positives = 12/23 (52%)
Frame = +3
Query: 321 PHKTERGKNALRRLRTYEGGPPP 389
P +R +N RR GGPPP
Sbjct: 221 PRNNDRSRNFERRRENMAGGPPP 243
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,790,957
Number of Sequences: 28952
Number of extensions: 177087
Number of successful extensions: 487
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 479
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 486
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 605614832
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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