Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00209
(748 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 116 3e-27
SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 116 3e-27
SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|c... 26 5.0
SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces ... 26 6.6
SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein Urb1|Schi... 25 8.7
SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9 |Schizos... 25 8.7
>SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 116 bits (280), Expect = 3e-27
Identities = 55/97 (56%), Positives = 60/97 (61%)
Frame = +1
Query: 241 EQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXX 420
E+ G HQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 56 EKAG-HQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVK 114
Query: 421 XXXXXXXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531
ARGH ++ P P
Sbjct: 115 VNQNEKRYAIASAVAASGVPSLLLARGHRIEEIPEVP 151
Score = 111 bits (266), Expect = 1e-25
Identities = 47/79 (59%), Positives = 65/79 (82%)
Frame = +3
Query: 510 EKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQR 689
E+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR
Sbjct: 145 EEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQR 204
Query: 690 KGPLIIFNKDQGLTRAFRN 746
+GPL++FN+D G+ +AFRN
Sbjct: 205 RGPLVVFNEDTGIVKAFRN 223
Score = 71.3 bits (167), Expect = 1e-13
Identities = 33/59 (55%), Positives = 43/59 (72%)
Frame = +2
Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 253
++ ARP VS+Y+ K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++A
Sbjct: 1 MAAARPTVSIYN-KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKA 58
>SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein
L2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 363
Score = 116 bits (280), Expect = 3e-27
Identities = 55/97 (56%), Positives = 60/97 (61%)
Frame = +1
Query: 241 EQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXX 420
E+ G HQTSAESWGTGRA+ARIPRV GGGTHRSGQ AFGNMCR GRMFAPTK WR+WH
Sbjct: 56 EKAG-HQTSAESWGTGRALARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVK 114
Query: 421 XXXXXXXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531
ARGH ++ P P
Sbjct: 115 VNQNEKRYAISSAVAASGVPSLLLARGHRIEEIPEVP 151
Score = 110 bits (265), Expect = 2e-25
Identities = 47/79 (59%), Positives = 65/79 (82%)
Frame = +3
Query: 510 EKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQR 689
E+IPE+PLVV D VQ KTK+AV L+ +KA+ D++KV S++LRAGKGK+RNRR +QR
Sbjct: 145 EEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQR 204
Query: 690 KGPLIIFNKDQGLTRAFRN 746
+GPL++FN+D G+ +AFRN
Sbjct: 205 RGPLVVFNEDAGIVKAFRN 223
Score = 72.1 bits (169), Expect = 8e-14
Identities = 34/59 (57%), Positives = 43/59 (72%)
Frame = +2
Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 253
++ ARP VS+YS K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++A
Sbjct: 1 MAAARPTVSIYS-KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKA 58
>SPBC577.12 |mug71||endoribonuclease |Schizosaccharomyces pombe|chr
2|||Manual
Length = 606
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = -2
Query: 138 APCTVSLFSEYTDTKGRATDRLISLL 61
A C V ++SEYT G ++ L++L+
Sbjct: 490 ADCVVKIWSEYTKNTGESSPVLVALV 515
>SPAC1D4.02c |||human GRASP protein homolog |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 345
Score = 25.8 bits (54), Expect = 6.6
Identities = 12/41 (29%), Positives = 17/41 (41%)
Frame = -2
Query: 522 RESFHNVSSSLNERWDAGSSNGCRQGRSPLSEVDATVPTPP 400
R+S V+ N W + GC G L + A + PP
Sbjct: 178 RDSTRQVTIVPNRHWGGNGAIGCGVGHGVLHRLPAPLSGPP 218
>SPCC14G10.02 ||SPCC18B5.13|ribosome biogenesis protein
Urb1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1568
Score = 25.4 bits (53), Expect = 8.7
Identities = 21/79 (26%), Positives = 37/79 (46%), Gaps = 2/79 (2%)
Frame = +2
Query: 77 LSVARPLVSVYSEKSETVQGAAKPLPFVFKAPI--RPDLVNDVHVSMSKNSRQPYCVSKE 250
L + R LV K ET+ + P+ + A I RP L+ + ++S + + + +
Sbjct: 753 LELIRELVEF--SKEETMATSQSPIRTLLSACINLRPFLLQEQYISFNMDFAEFLIDTNA 810
Query: 251 AVTKPVLNHGVLDVLSPEF 307
++ V+N VLD L F
Sbjct: 811 SIKGSVVNTCVLDFLYKSF 829
>SPAC23H4.02 |ppk9||serine/threonine protein kinase Ppk9
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 532
Score = 25.4 bits (53), Expect = 8.7
Identities = 12/37 (32%), Positives = 19/37 (51%)
Frame = +1
Query: 76 SIGSPTFSVGVLREE*DGAGCSQAPPVRVQGAHPSGP 186
SI SP+ SVG + + D + S + P+ + G P
Sbjct: 312 SITSPSSSVGQIPQPTDHSALSPSKPMSISGTESPNP 348
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,865,736
Number of Sequences: 5004
Number of extensions: 56007
Number of successful extensions: 149
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 137
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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