Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00209
(748 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 118 3e-27
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 118 3e-27
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 31 1.1
At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like... 30 1.9
At1g26400.1 68414.m03220 FAD-binding domain-containing protein s... 29 2.5
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 3.3
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 3.3
At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin; g... 29 3.3
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 29 4.3
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 29 4.3
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 29 4.3
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 29 4.3
At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein 29 4.3
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 7.6
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 7.6
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 10.0
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 118 bits (285), Expect = 3e-27
Identities = 54/92 (58%), Positives = 58/92 (63%)
Frame = +1
Query: 256 HQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXX 435
HQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 66 HQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNM 125
Query: 436 XXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531
ARGH ++ P P
Sbjct: 126 KRHAIVSAIAATAVPALVMARGHKIENVPEMP 157
Score = 80.2 bits (189), Expect = 1e-15
Identities = 37/80 (46%), Positives = 53/80 (66%), Gaps = 1/80 (1%)
Frame = +3
Query: 510 EKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQR 689
E +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I R
Sbjct: 151 ENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISR 210
Query: 690 KGPLIIFNKDQG-LTRAFRN 746
KGPL++F + + +AFRN
Sbjct: 211 KGPLVVFGTEGAKIVKAFRN 230
Score = 60.1 bits (139), Expect = 2e-09
Identities = 31/60 (51%), Positives = 38/60 (63%), Gaps = 2/60 (3%)
Frame = +2
Query: 80 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEA 253
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY VSK+A
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSKKA 64
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 118 bits (285), Expect = 3e-27
Identities = 54/92 (58%), Positives = 58/92 (63%)
Frame = +1
Query: 256 HQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHXXXXXXX 435
HQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWH
Sbjct: 65 HQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVNVNM 124
Query: 436 XXXXXXXXXXXXXXXXXXQARGHIMKRFPSFP 531
ARGH ++ P P
Sbjct: 125 KRHAIVSAIAATAVPALVMARGHKIENVPEMP 156
Score = 79.0 bits (186), Expect = 3e-15
Identities = 36/80 (45%), Positives = 53/80 (66%), Gaps = 1/80 (1%)
Frame = +3
Query: 510 EKIPELPLVVADKVQEINKTKQAVIFLRRLKAWSDILKVYKSQRLRAGKGKMRNRRRIQR 689
E +PE+PLVV+D + + KT A+ L+++ A+ D K S +R GKGKMRNRR I R
Sbjct: 150 ENVPEMPLVVSDSAEAVEKTSAAIKVLKQIGAYDDAEKAKNSIGIRPGKGKMRNRRYISR 209
Query: 690 KGPLIIFNKD-QGLTRAFRN 746
KGPL+++ + + +AFRN
Sbjct: 210 KGPLVVYGTEGSKIVKAFRN 229
Score = 62.1 bits (144), Expect = 4e-10
Identities = 31/63 (49%), Positives = 42/63 (66%), Gaps = 2/63 (3%)
Frame = +2
Query: 71 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVS 244
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY VS
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 245 KEA 253
K+A
Sbjct: 61 KKA 63
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 30.7 bits (66), Expect = 1.1
Identities = 21/70 (30%), Positives = 35/70 (50%)
Frame = +2
Query: 95 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAVTKPVLN 274
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+ + V
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICIYALGRMRSVWG 408
Query: 275 HGVLDVLSPE 304
LD PE
Sbjct: 409 EDALD-FKPE 417
>At1g49740.1 68414.m05578 expressed protein similar to MAP3K-like
protein kinase GB:CAB16796 GI:4006878 from [Arabidopsis
thaliana]
Length = 359
Score = 29.9 bits (64), Expect = 1.9
Identities = 19/57 (33%), Positives = 26/57 (45%)
Frame = +1
Query: 226 AALLREQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTK 396
A+LL C+Q + + W AV R GGG ++ A GN+ G FA K
Sbjct: 297 ASLLESIKTCYQAAGQRWPNFIAVDFYKRSDGGGAPQAVDVANGNLICGCDNFAACK 353
>At1g26400.1 68414.m03220 FAD-binding domain-containing protein
similar to SP|P30986 reticuline oxidase precursor
(Berberine-bridge-forming enzyme) (BBE)
(Tetrahydroprotoberberine synthase) [Eschscholzia
californica]; contains PF01565 FAD binding domain
Length = 527
Score = 29.5 bits (63), Expect = 2.5
Identities = 13/25 (52%), Positives = 18/25 (72%)
Frame = -1
Query: 574 CLVLLISWTLSATTRGSSGIFS*CV 500
CLVLL+S +A T+ SGIF+ C+
Sbjct: 9 CLVLLVSILRAAVTKPDSGIFTGCL 33
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 385 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 266
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 3.3
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -1
Query: 385 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 266
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g07520.1 68418.m00861 glycine-rich protein (GRP18) Oleosin;
glycine-rich protein 18 (GRP18) PMID:11431566;
Length = 228
Score = 29.1 bits (62), Expect = 3.3
Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 3/64 (4%)
Frame = +1
Query: 220 LEAALLREQGGCHQTSAESWGTGRAVARIPRVRGGGTHRSGQ---GAFGNMCRGGRMFAP 390
+ AL+R + G T AE + + + ++P G G G+ G FGN GG F
Sbjct: 76 MAVALIRRRMGAKPT-AEGTSSAQPLLKLPVYGGYGGFWGGKKFSGTFGNKPGGGNPFGD 134
Query: 391 TKPW 402
W
Sbjct: 135 ISKW 138
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 146 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAVTKPVLNHGVLDVLSPE 304
P+PF K+P +PD++ H + NSR +C+ K V ++ PE
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCLYSLGRMKSVWGEDAME-FKPE 434
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 146 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAVTKPVLNHGVLDVLSPE 304
P+PF K+P +PD++ H + NS+ +C+ K V L+ PE
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCLYSLGRMKSVWGEDALE-FKPE 907
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 28.7 bits (61), Expect = 4.3
Identities = 18/46 (39%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = -2
Query: 522 RESFHNVSSSLN-ERWDAGSSNGCRQGRSPLSEVDATVP-TPPGLR 391
+E N+ +L E W+ SNG ++ +SP S AT TPP LR
Sbjct: 645 KEQIENLKRALGTEEWN-NVSNGSKEIKSPFSRPIATTERTPPRLR 689
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +3
Query: 369 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 467
WW +V LA+ APS P + L SR C
Sbjct: 465 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 497
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 28.7 bits (61), Expect = 4.3
Identities = 13/33 (39%), Positives = 16/33 (48%)
Frame = +3
Query: 369 WWTYVRPHEALAALAPSRQPPTAESGLGGSRCC 467
WW +V LA+ APS P + L SR C
Sbjct: 464 WWNWVDRQNPLASPAPSYSQPQRDFRLTPSRLC 496
>At1g53120.1 68414.m06015 RNA-binding S4 domain-containing protein
Length = 320
Score = 28.7 bits (61), Expect = 4.3
Identities = 15/63 (23%), Positives = 31/63 (49%)
Frame = -3
Query: 701 EGSLTLDTTTVAHFTLTSTKTLRLVHLKDIRPCLEAPQEDDSLFGLVDLLDFVGYNQGKL 522
E SL +D A F ++ +K + L+ KD+R ++ ++ D++ G + K+
Sbjct: 242 EASLRIDAVASAGFKISRSKLVDLISSKDVRVNWATVTKNGTIVKTGDVVSVSGKGRLKI 301
Query: 521 GNL 513
G +
Sbjct: 302 GEI 304
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -1
Query: 373 HHDTCYRRHPDRTYEYHHHGHAEF 302
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 7.6
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -3
Query: 149 GAWLHPAPSHSSLSTPTLKVGLPID--SFRYFSEAIPPKY 36
G W+H SH+ S+ L VG+ + Y+S + P +Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 10.0
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -1
Query: 373 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 290
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,791,919
Number of Sequences: 28952
Number of extensions: 335937
Number of successful extensions: 1155
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1073
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1143
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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