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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00205
         (481 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein L27|Schizosacch...   113   2e-26
SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein L27|Schizosac...   108   4e-25
SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces p...    31   0.068
SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce...    27   1.1  
SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit ...    27   1.9  
SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces pomb...    26   2.6  
SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10 |...    26   3.4  
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr...    26   3.4  
SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase Ceg1|Schizosacc...    25   4.5  
SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr 1|||...    25   5.9  
SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyce...    25   5.9  
SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr ...    25   5.9  
SPBC211.03c |||guanyl-nucleotide exchange factor|Schizosaccharom...    25   5.9  
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr...    25   7.8  

>SPCC74.05 |rpl2702|rpl27-2|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 136

 Score =  113 bits (271), Expect = 2e-26
 Identities = 46/79 (58%), Positives = 63/79 (79%)
 Frame = +2

Query: 17  MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 196
           M KI+KPGKV LV  GR+AG+K ++++N D+G+   P+GHA VAG++RYP KV K MG  
Sbjct: 1   MVKILKPGKVALVTRGRFAGKKVVILQNVDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60

Query: 197 KIHKRSKIKPFVKVVNYNH 253
           +I KRS++KPF+KV+NYNH
Sbjct: 61  RIAKRSRVKPFIKVINYNH 79



 Score = 46.0 bits (104), Expect = 3e-06
 Identities = 21/57 (36%), Positives = 33/57 (57%), Gaps = 2/57 (3%)
 Frame = +1

Query: 253 LMPTRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 417
           LMPTRY ++    K   +     +P++R   +   +  FEE+Y++GK+ WFF  LRF
Sbjct: 80  LMPTRYALELDNLKGLVTPTTFSEPSQRSAAKKTVKNTFEEKYQTGKSAWFFTPLRF 136


>SPBC685.07c |rpl2701|rpl27-1|60S ribosomal protein
           L27|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 136

 Score =  108 bits (260), Expect = 4e-25
 Identities = 44/79 (55%), Positives = 62/79 (78%)
 Frame = +2

Query: 17  MGKIMKPGKVVLVLSGRYAGRKAIVVKNYDEGTSDKPYGHAFVAGIDRYPRKVHKRMGKN 196
           M KI+KPGKV L+  GR+AG+K ++++  D+G+   P+GHA VAG++RYP KV K MG  
Sbjct: 1   MVKILKPGKVALITRGRFAGKKVVILQAIDQGSKSHPFGHAVVAGVERYPLKVTKSMGAK 60

Query: 197 KIHKRSKIKPFVKVVNYNH 253
           +I +RS++KPF+KVVNYNH
Sbjct: 61  RIARRSRVKPFIKVVNYNH 79



 Score = 52.8 bits (121), Expect = 3e-08
 Identities = 25/57 (43%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
 Frame = +1

Query: 253 LMPTRYTVDFSFEK--FSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLRF 417
           LMPTRY ++    K   +A   K+P +R   R   +  FEE+Y+SGK+ WFF  LRF
Sbjct: 80  LMPTRYALELDNLKGLITADTFKEPTQRSAARKTVKKTFEEKYQSGKSAWFFTPLRF 136


>SPCC622.18 |rpl6||60S ribosomal protein L6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 195

 Score = 31.5 bits (68), Expect = 0.068
 Identities = 10/34 (29%), Positives = 23/34 (67%)
 Frame = +2

Query: 8   PSKMGKIMKPGKVVLVLSGRYAGRKAIVVKNYDE 109
           P+K+   + PG V ++L+GR+ G++ +V+   ++
Sbjct: 45  PTKLRASLAPGTVCILLAGRFRGKRVVVLSQLED 78


>SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 134

 Score = 27.5 bits (58), Expect = 1.1
 Identities = 11/28 (39%), Positives = 18/28 (64%)
 Frame = +2

Query: 23  KIMKPGKVVLVLSGRYAGRKAIVVKNYD 106
           + ++ G+VVLV  G Y G+ A++V   D
Sbjct: 6   RYVEVGRVVLVTKGEYTGKLAVIVDIVD 33


>SPBC106.06 |cct4||chaperonin-containing T-complex delta subunit
           Cct4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 527

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = +3

Query: 384 EEQVVLPEA*VLSVILKENKRYVSNEKKKKKK 479
           E QVV+ +   +  ILKE ++Y+ N  KK KK
Sbjct: 250 ENQVVVNDYRQMDKILKEERQYLLNMCKKIKK 281


>SPAC144.08 |||DNAJ domain protein Jac1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 225

 Score = 26.2 bits (55), Expect = 2.6
 Identities = 11/22 (50%), Positives = 13/22 (59%)
 Frame = +2

Query: 188 GKNKIHKRSKIKPFVKVVNYNH 253
           G + I  R K  P  K+VNYNH
Sbjct: 28  GNHWIFARFKFYPLNKIVNYNH 49


>SPAC25G10.04c |rec10|rec20|meiotic recombination protein Rec10
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 791

 Score = 25.8 bits (54), Expect = 3.4
 Identities = 11/30 (36%), Positives = 15/30 (50%)
 Frame = -2

Query: 123 LSEVPSS*FLTTIALRPAYRPLKTSTTLPG 34
           L E+P + F  T+    AY P   S T+ G
Sbjct: 689 LEEIPYNKFFKTVEKNEAYNPSSKSATIDG 718


>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1517

 Score = 25.8 bits (54), Expect = 3.4
 Identities = 15/38 (39%), Positives = 19/38 (50%)
 Frame = -1

Query: 217  LGPLVDFILSHPLVHFPGVPVDTSDESMPVRLVGGTFV 104
            LGP+ +    + L HFP    DT + S P    GGT V
Sbjct: 1364 LGPIGETFEVNGLSHFPSDIEDTIERSHPRIARGGTAV 1401


>SPBC2F12.08c |ceg1|pce1|mRNA guanylyltransferase
           Ceg1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 402

 Score = 25.4 bits (53), Expect = 4.5
 Identities = 14/45 (31%), Positives = 19/45 (42%)
 Frame = +1

Query: 280 FSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKNKWFFQKLR 414
           +SF  F   D K+  K K        R  E Y   +N+W F + R
Sbjct: 284 YSFFAFMYVDEKEWEKLKSFNVPLSERIVECYLDDENRWRFLRFR 328


>SPAC11D3.14c |||oxoprolinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1260

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 11/24 (45%), Positives = 15/24 (62%)
 Frame = +2

Query: 170 KVHKRMGKNKIHKRSKIKPFVKVV 241
           K+ KR+G   I   SK+ P VK+V
Sbjct: 205 KIAKRIGFTDISLSSKLTPMVKIV 228


>SPAC22F3.06c |lon1||Lon protease homolog Lon1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1067

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 23/105 (21%), Positives = 45/105 (42%), Gaps = 10/105 (9%)
 Frame = +1

Query: 67   VRGSQGYRSQELRRRYLRQAVRACFRRWYRQVPPE----------SAQEDGKE*NPQEVQ 216
            ++G   Y + E   R L++++   FR+    +  E          S  + GK+ +PQ  +
Sbjct: 751  IKGLISYYAHESGVRNLKKSIEKIFRKTSFSIVKEIDDELNSKEKSTGKSGKKTSPQSSE 810

Query: 217  DKAFREGCKL*SLMPTRYTVDFSFEKFSAKDLKDPAKRKKLRFNT 351
            D A +E   +   +P +  ++   EK   K L  P    +  ++T
Sbjct: 811  DAANKEASSVPLKVPDKVNIEIE-EKDLTKYLGPPIYTSQRLYDT 854


>SPAC57A7.05 |||conserved protein |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1337

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 11/39 (28%), Positives = 19/39 (48%)
 Frame = +1

Query: 274 VDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEERYKSGKN 390
           + F       K L DP+ R+K  F ++   + +Y+  KN
Sbjct: 150 LQFQIPSNEKKSLDDPSPRRKPSFFSKSNLKRKYRYLKN 188


>SPBC211.03c |||guanyl-nucleotide exchange
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1462

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 10/33 (30%), Positives = 18/33 (54%)
 Frame = +1

Query: 274 VDFSFEKFSAKDLKDPAKRKKLRFNTRVRFEER 372
           VD++ E+F      DP+     + NT ++ E+R
Sbjct: 821 VDYAIERFCQFTALDPSSVPGTQLNTAIKVEDR 853


>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
            3|||Manual
          Length = 1516

 Score = 24.6 bits (51), Expect = 7.8
 Identities = 10/30 (33%), Positives = 18/30 (60%)
 Frame = -3

Query: 305  FALNFSKLKSTV*RVGISDYSLQPSRKALS 216
            +ALNF ++K+ + R   +DY     +K +S
Sbjct: 1393 YALNFLQIKTLLHRYDYADYEAHVPKKTMS 1422


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,934,783
Number of Sequences: 5004
Number of extensions: 39361
Number of successful extensions: 139
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 134
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 139
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 184020746
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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