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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00203
         (762 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g38220.1 68417.m05394 aminoacylase, putative / N-acyl-L-amino...    72   4e-13
At4g38220.2 68417.m05395 aminoacylase, putative / N-acyl-L-amino...    71   8e-13
At1g44820.1 68414.m05134 aminoacylase, putative / N-acyl-L-amino...    69   3e-12
At1g44180.1 68414.m05103 aminoacylase, putative / N-acyl-L-amino...    66   2e-11
At2g12505.1 68415.m01352 hypothetical protein                          31   0.63 
At3g47230.1 68416.m05128 expressed protein                             28   5.9  

>At4g38220.1 68417.m05394 aminoacylase, putative /
           N-acyl-L-amino-acid amidohydrolase, putative similar to
           aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
           ACY-1)[Homo sapiens] SWISS-PROT:Q03154
          Length = 430

 Score = 72.1 bits (169), Expect = 4e-13
 Identities = 35/80 (43%), Positives = 48/80 (60%), Gaps = 2/80 (2%)
 Frame = +2

Query: 2   YEFEQK-DPYTTPTQLDDANIYWKAFKQTAQELR-MSIKPQTFTGGTDSRYLRELGIPAL 175
           +EF+QK       T  DD+N +W   +   +E    + KP+ F   TD+RY R+ G+PA 
Sbjct: 320 FEFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPAF 379

Query: 176 GFSPIHNTTPALHEHNEHLG 235
           GFSPI NT   LH+HNE+LG
Sbjct: 380 GFSPISNTPSLLHDHNEYLG 399


>At4g38220.2 68417.m05395 aminoacylase, putative /
           N-acyl-L-amino-acid amidohydrolase, putative similar to
           aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
           ACY-1)[Homo sapiens] SWISS-PROT:Q03154
          Length = 433

 Score = 70.9 bits (166), Expect = 8e-13
 Identities = 31/67 (46%), Positives = 42/67 (62%), Gaps = 1/67 (1%)
 Frame = +2

Query: 38  TQLDDANIYWKAFKQTAQELR-MSIKPQTFTGGTDSRYLRELGIPALGFSPIHNTTPALH 214
           T  DD+N +W   +   +E    + KP+ F   TD+RY R+ G+PA GFSPI NT   LH
Sbjct: 336 TAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLH 395

Query: 215 EHNEHLG 235
           +HNE+LG
Sbjct: 396 DHNEYLG 402


>At1g44820.1 68414.m05134 aminoacylase, putative /
           N-acyl-L-amino-acid amidohydrolase, putative similar to
           aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase
           ACY-1)[Homo sapiens] SWISS-PROT:Q03154
          Length = 438

 Score = 69.3 bits (162), Expect = 3e-12
 Identities = 32/72 (44%), Positives = 45/72 (62%), Gaps = 1/72 (1%)
 Frame = +2

Query: 38  TQLDDANIYWKAFKQTAQELRMSI-KPQTFTGGTDSRYLRELGIPALGFSPIHNTTPALH 214
           T ++D+N +W  FKQ  + +   + KP+     TDSR++R LGIP  GFSP+ NT   LH
Sbjct: 343 TPVNDSNPWWSIFKQAVEAMGGKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLH 402

Query: 215 EHNEHLGLDVFI 250
           +HNE L   VF+
Sbjct: 403 DHNEFLKDTVFM 414


>At1g44180.1 68414.m05103 aminoacylase, putative /
           N-acyl-L-amino-acid amidohydrolase, putative similar to
           aminoacylase-1 (N-acyl-L-amino-acid amidohydrolase,
           ACY-1) [Homo sapiens] SWISS-PROT:Q03154
          Length = 435

 Score = 66.5 bits (155), Expect = 2e-11
 Identities = 32/72 (44%), Positives = 43/72 (59%), Gaps = 1/72 (1%)
 Frame = +2

Query: 38  TQLDDANIYWKAFKQTAQELRMSI-KPQTFTGGTDSRYLRELGIPALGFSPIHNTTPALH 214
           T  +D N +W  FKQ  +     + KP+     TDSR++R LGIP LGFSP+ NT   +H
Sbjct: 345 TTTNDTNPWWSIFKQAVEATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVH 404

Query: 215 EHNEHLGLDVFI 250
           +HNE L   VF+
Sbjct: 405 DHNEFLKDTVFM 416


>At2g12505.1 68415.m01352 hypothetical protein
          Length = 344

 Score = 31.5 bits (68), Expect = 0.63
 Identities = 24/73 (32%), Positives = 33/73 (45%), Gaps = 8/73 (10%)
 Frame = +3

Query: 369 PIRPIVSRITIHWPSFYNV-VTGKTLALPNLIALQHIPLSPAG------VIAKSPHRSPF 527
           PIRP + +      S Y +    KT  LP+  +  H  + P G      V   SP R+PF
Sbjct: 183 PIRPTIGQADPIRRSVYQIRFVPKTNGLPSPFSTLHRTVGPGGSMRISSVELYSPSRTPF 242

Query: 528 PT-VAHLNGEWQI 563
           PT    + GEW +
Sbjct: 243 PTNNFRMWGEWML 255


>At3g47230.1 68416.m05128 expressed protein
          Length = 277

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 22/66 (33%), Positives = 28/66 (42%), Gaps = 7/66 (10%)
 Frame = +3

Query: 357 GARYPIRPIVSRITIHWPSFYNV-VTGKTLALPNLIALQHIPLSPAG------VIAKSPH 515
           G   PIRP +        S Y +    KT  LP+  +  H  + P G      V   SP 
Sbjct: 32  GLADPIRPTIGLADPIRRSVYQIRFVSKTNGLPSPSSTLHRTVGPGGSMRISSVELYSPS 91

Query: 516 RSPFPT 533
           R+PFPT
Sbjct: 92  RTPFPT 97


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,135,665
Number of Sequences: 28952
Number of extensions: 331779
Number of successful extensions: 793
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 773
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 791
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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