BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00202 (791 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13210.1 68414.m01532 haloacid dehalogenase-like hydrolase fa... 30 2.0 At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearl... 28 6.2 At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 d... 28 8.2 At3g25610.1 68416.m03188 haloacid dehalogenase-like hydrolase fa... 28 8.2 >At1g13210.1 68414.m01532 haloacid dehalogenase-like hydrolase family protein similar to Potential phospholipid-transporting ATPase (EC 3.6.3.1) (Chromaffin granule ATPase) from {Homo sapiens} SP|Q9Y2Q0, {Mus musculus} SP|P98200, {Bos taurus} SP|Q29449; contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase; ESTs gb|T45045 and gb|AA394473 come from this gene Length = 1203 Score = 29.9 bits (64), Expect = 2.0 Identities = 15/35 (42%), Positives = 20/35 (57%), Gaps = 2/35 (5%) Frame = +3 Query: 576 TFHFH--KFPTSVLQAHSRDSKLSNVQNINTVIIF 674 T HF + P S+ Q RDSKL N + I V++F Sbjct: 241 TLHFEEQRLPLSITQLLLRDSKLRNTEYIYGVVVF 275 >At1g70320.1 68414.m08090 ubiquitin-protein ligase 2 (UPL2) nearly identical to ubiquitin-protein ligase 2 [Arabidopsis thaliana] GI:7108523; E3, HECT-domain protein family; similar to ubiquitin-protein ligase 2 GI:7108523 from [Arabidopsis thaliana] Length = 3658 Score = 28.3 bits (60), Expect = 6.2 Identities = 16/60 (26%), Positives = 25/60 (41%) Frame = -1 Query: 713 IFTFIRKKKSFYQEYDDSVDILDI*QFRISRMCLKH*SRKLMEMKRQPKIRATVTSSGHK 534 + +F + F E V D +I +CL+ + RQP + VTS GH+ Sbjct: 286 VVSFFNGEPEFVNELVTLVSYEDTVPAKIRILCLQSLVALSQDRTRQPTVLTAVTSGGHR 345 >At3g60240.1 68416.m06732 MIF4G domain-containing protein / MA3 domain-containing protein similar to eukaryotic protein synthesis initiation factor [Homo sapiens] GI:3941724; contains Pfam profiles PF02854: MIF4G domain, PF02847: MA3 domain Length = 1544 Score = 27.9 bits (59), Expect = 8.2 Identities = 15/26 (57%), Positives = 18/26 (69%) Frame = -1 Query: 128 VTHYRSAPQGRCTALPHRTQILNLSR 51 VTH RS+PQ R TA HR + +LSR Sbjct: 1298 VTHGRSSPQERGTAYVHR-EFASLSR 1322 >At3g25610.1 68416.m03188 haloacid dehalogenase-like hydrolase family protein similar to Potential phospholipid-transporting ATPase (EC 3.6.3.1) from Mus musculus [SP|P98200, SP|P70704], {Bos taurus} SP|Q29449; contains InterPro accession IPR005834: Haloacid dehalogenase-like hydrolase Length = 1202 Score = 27.9 bits (59), Expect = 8.2 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +3 Query: 573 LTFHFHKFPTSVLQAHSRDSKLSNVQNINTVIIF 674 L +FP S+ Q RDSKL N + + ++F Sbjct: 244 LALEEERFPLSIQQILLRDSKLRNTEYVYGAVVF 277 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,885,881 Number of Sequences: 28952 Number of extensions: 270789 Number of successful extensions: 484 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 473 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 484 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1785055200 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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