Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00196
(659 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g04430.2 68418.m00438 KH domain-containing protein NOVA, puta... 36 0.032
At5g04430.1 68418.m00437 KH domain-containing protein NOVA, puta... 36 0.032
At4g10070.1 68417.m01647 KH domain-containing protein DNA-direct... 36 0.032
At2g25970.1 68415.m03117 KH domain-containing protein 35 0.055
At3g12130.1 68416.m01509 KH domain-containing protein / zinc fin... 33 0.13
At1g14170.1 68414.m01675 KH domain-containing protein location o... 32 0.29
At2g38610.2 68415.m04743 KH domain-containing protein 31 0.51
At2g38610.1 68415.m04742 KH domain-containing protein 31 0.51
At5g06770.1 68418.m00765 KH domain-containing protein / zinc fin... 30 1.2
At3g08620.1 68416.m01001 KH domain-containing protein 30 1.2
At5g46190.1 68418.m05681 KH domain-containing protein strong sim... 29 2.1
At5g23690.1 68418.m02777 polynucleotide adenylyltransferase fami... 29 2.1
At1g75430.1 68414.m08762 homeodomain-containing protein contains... 29 2.7
At5g53060.1 68418.m06592 KH domain-containing protein 29 3.6
At5g09560.1 68418.m01107 KH domain-containing protein various pr... 28 4.8
At2g40116.1 68415.m04933 phosphoinositide-specific phospholipase... 28 4.8
At1g33680.1 68414.m04166 KH domain-containing protein similar to... 27 8.4
>At5g04430.2 68418.m00438 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 334
Score = 35.5 bits (78), Expect = 0.032
Identities = 24/90 (26%), Positives = 40/90 (44%), Gaps = 1/90 (1%)
Frame = +2
Query: 212 AERRQGRHGVRTAVADRPEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCELDRRCQ 391
A+R H A + + F + G VIG+ G+ I + A+SGA +L R +
Sbjct: 16 AKRSPEPHDSSEADSAEKPTHIRFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQE 75
Query: 392 SNDRNT-RTFIIRGHPEAVETCTRIIMEKV 478
T R +I G + V +I++K+
Sbjct: 76 FFPGTTDRIIMISGSIKEVVNGLELILDKL 105
Score = 31.9 bits (69), Expect = 0.39
Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 275 VTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCEL-DRRCQSNDRNTRTFIIRGHPEAVET 451
VT + GLV+GR G I +I +GA ++ DR + R I G A++
Sbjct: 257 VTIGVADEHIGLVLGRGGRNIMEITQMTGARIKISDRGDFMSGTTDRKVSITGPQRAIQQ 316
Query: 452 CTRIIMEKV 478
+I +KV
Sbjct: 317 AETMIKQKV 325
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/60 (25%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQEHDEEPG--DKLCYLQGKPHQLDQARQMIEDLISSVKRCEE 176
G I + QA +G R+Q ++ + PG D++ + G ++ ++I D + S E+
Sbjct: 54 GSTITEFQAKSGARIQLSRNQEFFPGTTDRIIMISGSIKEVVNGLELILDKLHSELHAED 113
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 263 PEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGA 364
P R+ + CG +IG+ GA IK +S A
Sbjct: 119 PRRRIRLVVPNSSCGGIIGKGGATIKSFIEESKA 152
>At5g04430.1 68418.m00437 KH domain-containing protein NOVA,
putative astrocytic NOVA-like RNA-binding protein, Homo
sapiens, U70477
Length = 313
Score = 35.5 bits (78), Expect = 0.032
Identities = 24/90 (26%), Positives = 40/90 (44%), Gaps = 1/90 (1%)
Frame = +2
Query: 212 AERRQGRHGVRTAVADRPEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCELDRRCQ 391
A+R H A + + F + G VIG+ G+ I + A+SGA +L R +
Sbjct: 16 AKRSPEPHDSSEADSAEKPTHIRFLVSNAAAGSVIGKGGSTITEFQAKSGARIQLSRNQE 75
Query: 392 SNDRNT-RTFIIRGHPEAVETCTRIIMEKV 478
T R +I G + V +I++K+
Sbjct: 76 FFPGTTDRIIMISGSIKEVVNGLELILDKL 105
Score = 31.9 bits (69), Expect = 0.39
Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 1/69 (1%)
Frame = +2
Query: 275 VTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCEL-DRRCQSNDRNTRTFIIRGHPEAVET 451
VT + GLV+GR G I +I +GA ++ DR + R I G A++
Sbjct: 236 VTIGVADEHIGLVLGRGGRNIMEITQMTGARIKISDRGDFMSGTTDRKVSITGPQRAIQQ 295
Query: 452 CTRIIMEKV 478
+I +KV
Sbjct: 296 AETMIKQKV 304
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/60 (25%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQEHDEEPG--DKLCYLQGKPHQLDQARQMIEDLISSVKRCEE 176
G I + QA +G R+Q ++ + PG D++ + G ++ ++I D + S E+
Sbjct: 54 GSTITEFQAKSGARIQLSRNQEFFPGTTDRIIMISGSIKEVVNGLELILDKLHSELHAED 113
Score = 27.9 bits (59), Expect = 6.3
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 263 PEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGA 364
P R+ + CG +IG+ GA IK +S A
Sbjct: 119 PRRRIRLVVPNSSCGGIIGKGGATIKSFIEESKA 152
>At4g10070.1 68417.m01647 KH domain-containing protein DNA-directed
RNA polymerase (EC 2.7.7.6) II largestchain - mouse,
PIR2:A28490
Length = 725
Score = 35.5 bits (78), Expect = 0.032
Identities = 21/71 (29%), Positives = 35/71 (49%), Gaps = 1/71 (1%)
Frame = +2
Query: 263 PEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCEL-DRRCQSNDRNTRTFIIRGHPE 439
PE ++ + K GL+IGR G IK + +SGA +L + + + RT I G
Sbjct: 271 PE-QIEIKVPNDKVGLIIGRGGETIKNMQTRSGARTQLIPQHAEGDGLKERTVRISGDKM 329
Query: 440 AVETCTRIIME 472
++ T +I +
Sbjct: 330 QIDIATDMIKD 340
>At2g25970.1 68415.m03117 KH domain-containing protein
Length = 632
Score = 34.7 bits (76), Expect = 0.055
Identities = 17/59 (28%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
Frame = +2
Query: 299 KCGLVIGRRGAIIKQINAQSGAHCELDRRCQSNDR-NTRTFIIRGHPEAVETCTRIIME 472
+ G++IG+ G IK + QSGA ++ R ++ TRT + G P+ + ++I +
Sbjct: 144 RVGVIIGKGGETIKYLQLQSGAKIQVTRDMDADPNCATRTVDLTGTPDQISKAEQLITD 202
Score = 32.3 bits (70), Expect = 0.29
Identities = 20/64 (31%), Positives = 32/64 (50%), Gaps = 2/64 (3%)
Frame = +2
Query: 299 KCGLVIGRRGAIIKQINAQSGAHCE-LDRRCQSNDRN-TRTFIIRGHPEAVETCTRIIME 472
K GL+IG+ G IK + A++GA + + D RT I G E +E +++ E
Sbjct: 240 KVGLIIGKGGETIKSMQAKTGARIQVIPLHLPPGDPTPERTLQIDGITEQIEHAKQLVNE 299
Query: 473 KVGG 484
+ G
Sbjct: 300 IISG 303
>At3g12130.1 68416.m01509 KH domain-containing protein / zinc finger
(CCCH type) family protein
Length = 248
Score = 33.5 bits (73), Expect = 0.13
Identities = 17/50 (34%), Positives = 28/50 (56%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQEHDEEPGDKLCYLQGKPHQLDQARQMIEDLI 152
G K+I TG ++ Q+H+ +P K L+G Q+ +A M++DLI
Sbjct: 133 GVSSKQICRQTGVKLSI-QDHERDPNLKNIVLEGTLEQISEASAMVKDLI 181
Score = 32.3 bits (70), Expect = 0.29
Identities = 16/67 (23%), Positives = 32/67 (47%)
Frame = +2
Query: 281 FTIHGPKCGLVIGRRGAIIKQINAQSGAHCELDRRCQSNDRNTRTFIIRGHPEAVETCTR 460
F++ G +IG+ G KQI Q+G +L + D N + ++ G E + +
Sbjct: 118 FSVDASLAGAIIGKGGVSSKQICRQTGV--KLSIQDHERDPNLKNIVLEGTLEQISEASA 175
Query: 461 IIMEKVG 481
++ + +G
Sbjct: 176 MVKDLIG 182
>At1g14170.1 68414.m01675 KH domain-containing protein location of
EST 219C14T7 , gb|N38506
Length = 454
Score = 32.3 bits (70), Expect = 0.29
Identities = 17/44 (38%), Positives = 22/44 (50%)
Frame = +2
Query: 233 HGVRTAVADRPEMRVTFTIHGPKCGLVIGRRGAIIKQINAQSGA 364
+ VR +AD E V F G VIG+ G I QI ++GA
Sbjct: 232 YAVRRDIADAREFCVCFICPAENVGGVIGKGGGFINQIRQETGA 275
>At2g38610.2 68415.m04743 KH domain-containing protein
Length = 286
Score = 31.5 bits (68), Expect = 0.51
Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQE---HDEEPGDKLCYLQGKPHQLDQARQMIE-DLISSV 161
G +K+++A TGCRV + D E DKL G H +Q +IE DL +S+
Sbjct: 161 GNSLKRVEATTGCRVFIRGKGSIKDPEKEDKLRGRPGYEHLNEQLHILIEADLPASI 217
>At2g38610.1 68415.m04742 KH domain-containing protein
Length = 286
Score = 31.5 bits (68), Expect = 0.51
Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 4/57 (7%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQE---HDEEPGDKLCYLQGKPHQLDQARQMIE-DLISSV 161
G +K+++A TGCRV + D E DKL G H +Q +IE DL +S+
Sbjct: 161 GNSLKRVEATTGCRVFIRGKGSIKDPEKEDKLRGRPGYEHLNEQLHILIEADLPASI 217
>At5g06770.1 68418.m00765 KH domain-containing protein / zinc finger
(CCCH type) family protein contains Pfam domains
PF00013: KH domain and PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 240
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/46 (30%), Positives = 28/46 (60%)
Frame = +3
Query: 15 KKIQADTGCRVQFNQEHDEEPGDKLCYLQGKPHQLDQARQMIEDLI 152
K+I +TG ++ ++H+ +P K+ L+G Q++ A M+ +LI
Sbjct: 137 KQICRETGAKLSI-KDHERDPNLKIIELEGTFEQINVASGMVRELI 181
Score = 27.9 bits (59), Expect = 6.3
Identities = 16/59 (27%), Positives = 29/59 (49%)
Frame = +2
Query: 305 GLVIGRRGAIIKQINAQSGAHCELDRRCQSNDRNTRTFIIRGHPEAVETCTRIIMEKVG 481
G +IG+ G KQI ++GA +L + D N + + G E + + ++ E +G
Sbjct: 126 GAIIGKGGIHSKQICRETGA--KLSIKDHERDPNLKIIELEGTFEQINVASGMVRELIG 182
>At3g08620.1 68416.m01001 KH domain-containing protein
Length = 283
Score = 30.3 bits (65), Expect = 1.2
Identities = 17/50 (34%), Positives = 27/50 (54%), Gaps = 3/50 (6%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQEHDEEPGDKLCYLQGKP---HQLDQARQMIE 143
G +K+++A TGCRV + + +K L+GKP H +Q +IE
Sbjct: 160 GNSLKRVEATTGCRVYIRGKGSIKDPEKEEKLKGKPGYEHLNEQLHILIE 209
>At5g46190.1 68418.m05681 KH domain-containing protein strong
similarity to unknown protein (pir||T04533)
Length = 644
Score = 29.5 bits (63), Expect = 2.1
Identities = 16/35 (45%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = +2
Query: 299 KCGLVIGRRGAIIKQINAQSGAHCEL-DRRCQSND 400
K G VIG+ G IK I SG+H E+ D R +D
Sbjct: 327 KIGRVIGKGGLTIKGIRQASGSHIEVNDSRTNHDD 361
Score = 29.1 bits (62), Expect = 2.7
Identities = 15/52 (28%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
Frame = +2
Query: 257 DRPEMR-VTFTIHGPKCGLVIGRRGAIIKQINAQSGAHCELDRRCQSNDRNT 409
DR + R + +C +VIG+ G+IIK I ++ A+ ++ + S+ +T
Sbjct: 145 DRDDFRECRLLVPSSQCSIVIGKSGSIIKNIRGRTRANVKVVSKDVSDPSHT 196
>At5g23690.1 68418.m02777 polynucleotide adenylyltransferase family
protein low similarity to SP|P13685 Poly(A) polymerase
(EC 2.7.7.19) {Escherichia coli O157:H7}; contains Pfam
profile PF01743: polyA polymerase family protein
Length = 527
Score = 29.5 bits (63), Expect = 2.1
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = -3
Query: 342 CFMMAPRLPMTRPHLGPWMVKVTRISGRSATAVRTPCRPCRRSAGPD 202
C ++ R P+ H+G +++V+ S + + R C+ S+G D
Sbjct: 134 CEIVGRRFPICHVHIGDDLIEVSSFSTSAQNSSRNTRTECKESSGSD 180
>At1g75430.1 68414.m08762 homeodomain-containing protein contains
'Homeobox' domain signature, Prosite:PS00027
Length = 297
Score = 29.1 bits (62), Expect = 2.7
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = -2
Query: 367 VGSGLSVYLLYDGTTSANDQAALGSVDGEGDAHLW 263
+ SGLS L+DG T+++ LG V G H W
Sbjct: 170 ISSGLSQLSLFDGNTTSSSLQRLGLVQGP-QRHAW 203
>At5g53060.1 68418.m06592 KH domain-containing protein
Length = 652
Score = 28.7 bits (61), Expect = 3.6
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = +2
Query: 290 HGPKCGLVIGRRGAIIKQINAQSGA 364
H K G VIG+ G IIK I +GA
Sbjct: 75 HDAKAGGVIGKSGTIIKSIRQHTGA 99
>At5g09560.1 68418.m01107 KH domain-containing protein various
predicted RNA binding proteins, Arabidopsis thaliana
Length = 563
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/60 (26%), Positives = 33/60 (55%), Gaps = 4/60 (6%)
Frame = +3
Query: 3 GKMIKKIQADTGCRVQFNQEHD----EEPGDKLCYLQGKPHQLDQARQMIEDLISSVKRC 170
G+++++I +TGC VQ + HD PGD + ++G ++L + + + S ++ C
Sbjct: 156 GELMQRILEETGCNVQL-RSHDLSICTNPGDVVLKIEG--NRLSAVMKALVSISSRLQAC 212
>At2g40116.1 68415.m04933 phosphoinositide-specific phospholipase C
family protein contains Pfam profile: PF00388
phosphatidylinositol-specific phospholipase C
Length = 613
Score = 28.3 bits (60), Expect = 4.8
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 1/57 (1%)
Frame = +2
Query: 260 RPEMRVTFTIH-GPKCGLVIGRRGAIIKQINAQSGAHCELDRRCQSNDRNTRTFIIR 427
+P + TIH G G V ++ ++ S + ELDR C SN ++ F R
Sbjct: 345 KPAYKRLITIHAGKPKGTVKEEMKVVVDKVRRLSLSEQELDRTCSSNSQDVVRFTQR 401
>At1g33680.1 68414.m04166 KH domain-containing protein similar to
FUSE binding protein 2 GB:AAC50892 GI:1575607 from [Homo
sapiens]
Length = 759
Score = 27.5 bits (58), Expect = 8.4
Identities = 14/57 (24%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +2
Query: 299 KCGLVIGRRGAIIKQINAQSGAHCELDRRCQSNDRNT-RTFIIRGHPEAVETCTRII 466
K G +IG+ G +++ + SGA ++ R +++ + R I G +E ++I
Sbjct: 242 KVGTLIGKGGEMVRYLQVNSGAKIQIRRDAEADPSSALRPVEIIGTVSCIEKAEKLI 298
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,054,642
Number of Sequences: 28952
Number of extensions: 211230
Number of successful extensions: 640
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 608
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 640
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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