BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00195
(705 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 4e-09
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 48 1e-06
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 41 2e-04
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 39 8e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.002
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 30 0.28
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 27 2.0
SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 27 3.5
SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 26 4.6
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 56.4 bits (130), Expect = 4e-09
Identities = 23/51 (45%), Positives = 34/51 (66%)
Frame = +3
Query: 102 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVV 254
+ DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ +
Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI 53
Score = 47.6 bits (108), Expect = 2e-06
Identities = 19/38 (50%), Positives = 29/38 (76%)
Frame = +2
Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370
KVDVD+ +IA+E +++MP+F KNG+K++E GAN
Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 48.0 bits (109), Expect = 1e-06
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +2
Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370
KV+VDE IAS + +MPTFVF +NGK++D +GAN
Sbjct: 57 KVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGAN 94
Score = 34.3 bits (75), Expect = 0.017
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +3
Query: 156 VVIDFMATWCGPCKMIGPKLDEIAAE 233
+ +D A WCGPCK I P ++A++
Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 44.4 bits (100), Expect = 2e-05
Identities = 17/44 (38%), Positives = 29/44 (65%)
Frame = +3
Query: 102 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 233
++ D TR++ DK+ V+DF A WCGPCK + P L++++ +
Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63
Score = 34.3 bits (75), Expect = 0.017
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = +2
Query: 260 VDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGANV 373
V+ D+ DIA + + ++PT V + G++LD GA+V
Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADV 109
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 40.7 bits (91), Expect = 2e-04
Identities = 19/63 (30%), Positives = 33/63 (52%)
Frame = +3
Query: 54 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 233
VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A
Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67
Query: 234 MSD 242
D
Sbjct: 68 FED 70
Score = 30.3 bits (65), Expect = 0.28
Identities = 10/25 (40%), Positives = 16/25 (64%)
Frame = +3
Query: 150 KLVVIDFMATWCGPCKMIGPKLDEI 224
K V+++F A WCG CK + P + +
Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183
Score = 29.9 bits (64), Expect = 0.37
Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%)
Frame = +2
Query: 257 KVDVDECEDIASEYNINSMPTFV-FVKNGKKLDEFSGA 367
K+D D D+A +Y+I PT + F +G + ++S A
Sbjct: 78 KIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 38.7 bits (86), Expect = 8e-04
Identities = 16/39 (41%), Positives = 27/39 (69%), Gaps = 2/39 (5%)
Frame = +3
Query: 150 KLVVIDFMATWCGPCKMIGPKLDEIAAEMSD--SIVVSR 260
K V+++F A WCG CK + P +++A E SD ++VV++
Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAK 412
Score = 37.5 bits (83), Expect = 0.002
Identities = 16/36 (44%), Positives = 23/36 (63%)
Frame = +2
Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSG 364
+VD E D+ SEY+I PT KNGK++ ++SG
Sbjct: 77 EVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSG 112
Score = 36.3 bits (80), Expect = 0.004
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = +3
Query: 147 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM 236
DK++++ F A WCG CK + P+ + A E+
Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADEL 68
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 37.5 bits (83), Expect = 0.002
Identities = 14/38 (36%), Positives = 24/38 (63%)
Frame = +2
Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370
K++ ++ DIA +++N++P FV + K L SGAN
Sbjct: 56 KIEAEKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93
Score = 35.1 bits (77), Expect = 0.010
Identities = 12/53 (22%), Positives = 28/53 (52%)
Frame = +3
Query: 90 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 248
MS+ I + + L +++++++F A W PCK + D+ A + +++
Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 30.3 bits (65), Expect = 0.28
Identities = 15/50 (30%), Positives = 26/50 (52%)
Frame = +3
Query: 108 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVVS 257
+S + + + G LVV F A WCG CK + P ++A+ + + V+
Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVT 84
>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 905
Score = 27.5 bits (58), Expect = 2.0
Identities = 16/50 (32%), Positives = 27/50 (54%)
Frame = +3
Query: 108 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVVS 257
+ D + RL EAG + +A CGPC + + D+IA+ ++I+ S
Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAILTS 475
>SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase
Ogm4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 778
Score = 26.6 bits (56), Expect = 3.5
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 7/57 (12%)
Frame = -3
Query: 607 YLLNCLITTGPI-FFAFQRLQTLKINIFKFITDVKT------FFLHLLKTYTCFFFF 458
+ L+C I+T + FF F + ++ D+KT FF H L + C FF
Sbjct: 237 FFLSCTISTKYVGFFTFLSIGLSVCLELWYLWDIKTGLTVERFFQHFLARFFCLIFF 293
>SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1029
Score = 26.2 bits (55), Expect = 4.6
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = -1
Query: 540 KLISSNSLQTLKHFF 496
KL+SSN+LQ + HF+
Sbjct: 412 KLVSSNTLQAMSHFY 426
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,668,874
Number of Sequences: 5004
Number of extensions: 50759
Number of successful extensions: 149
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 131
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 149
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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