BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00195 (705 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 4e-09 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 48 1e-06 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 41 2e-04 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 39 8e-04 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 38 0.002 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 30 0.28 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 27 2.0 SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizo... 27 3.5 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 26 4.6 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 56.4 bits (130), Expect = 4e-09 Identities = 23/51 (45%), Positives = 34/51 (66%) Frame = +3 Query: 102 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVV 254 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI 53 Score = 47.6 bits (108), Expect = 2e-06 Identities = 19/38 (50%), Positives = 29/38 (76%) Frame = +2 Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370 KVDVD+ +IA+E +++MP+F KNG+K++E GAN Sbjct: 54 KVDVDQLSEIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 48.0 bits (109), Expect = 1e-06 Identities = 21/38 (55%), Positives = 28/38 (73%) Frame = +2 Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370 KV+VDE IAS + +MPTFVF +NGK++D +GAN Sbjct: 57 KVNVDEQRQIASGLGVKAMPTFVFFENGKQIDMLTGAN 94 Score = 34.3 bits (75), Expect = 0.017 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +3 Query: 156 VVIDFMATWCGPCKMIGPKLDEIAAE 233 + +D A WCGPCK I P ++A++ Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 44.4 bits (100), Expect = 2e-05 Identities = 17/44 (38%), Positives = 29/44 (65%) Frame = +3 Query: 102 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 233 ++ D TR++ DK+ V+DF A WCGPCK + P L++++ + Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63 Score = 34.3 bits (75), Expect = 0.017 Identities = 14/38 (36%), Positives = 24/38 (63%) Frame = +2 Query: 260 VDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGANV 373 V+ D+ DIA + + ++PT V + G++LD GA+V Sbjct: 72 VNADKFSDIAQKNGVYALPTMVLFRKGQELDRIVGADV 109 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 40.7 bits (91), Expect = 2e-04 Identities = 19/63 (30%), Positives = 33/63 (52%) Frame = +3 Query: 54 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 233 VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67 Query: 234 MSD 242 D Sbjct: 68 FED 70 Score = 30.3 bits (65), Expect = 0.28 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +3 Query: 150 KLVVIDFMATWCGPCKMIGPKLDEI 224 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 Score = 29.9 bits (64), Expect = 0.37 Identities = 13/38 (34%), Positives = 22/38 (57%), Gaps = 1/38 (2%) Frame = +2 Query: 257 KVDVDECEDIASEYNINSMPTFV-FVKNGKKLDEFSGA 367 K+D D D+A +Y+I PT + F +G + ++S A Sbjct: 78 KIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 38.7 bits (86), Expect = 8e-04 Identities = 16/39 (41%), Positives = 27/39 (69%), Gaps = 2/39 (5%) Frame = +3 Query: 150 KLVVIDFMATWCGPCKMIGPKLDEIAAEMSD--SIVVSR 260 K V+++F A WCG CK + P +++A E SD ++VV++ Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAK 412 Score = 37.5 bits (83), Expect = 0.002 Identities = 16/36 (44%), Positives = 23/36 (63%) Frame = +2 Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSG 364 +VD E D+ SEY+I PT KNGK++ ++SG Sbjct: 77 EVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSG 112 Score = 36.3 bits (80), Expect = 0.004 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +3 Query: 147 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM 236 DK++++ F A WCG CK + P+ + A E+ Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADEL 68 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 37.5 bits (83), Expect = 0.002 Identities = 14/38 (36%), Positives = 24/38 (63%) Frame = +2 Query: 257 KVDVDECEDIASEYNINSMPTFVFVKNGKKLDEFSGAN 370 K++ ++ DIA +++N++P FV + K L SGAN Sbjct: 56 KIEAEKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93 Score = 35.1 bits (77), Expect = 0.010 Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = +3 Query: 90 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 248 MS+ I + + L +++++++F A W PCK + D+ A + +++ Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 30.3 bits (65), Expect = 0.28 Identities = 15/50 (30%), Positives = 26/50 (52%) Frame = +3 Query: 108 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVVS 257 +S + + + G LVV F A WCG CK + P ++A+ + + V+ Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVT 84 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 27.5 bits (58), Expect = 2.0 Identities = 16/50 (32%), Positives = 27/50 (54%) Frame = +3 Query: 108 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSIVVS 257 + D + RL EAG + +A CGPC + + D+IA+ ++I+ S Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAILTS 475 >SPBC16C6.09 |ogm4|oma4|protein O-mannosyltransferase Ogm4|Schizosaccharomyces pombe|chr 2|||Manual Length = 778 Score = 26.6 bits (56), Expect = 3.5 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 7/57 (12%) Frame = -3 Query: 607 YLLNCLITTGPI-FFAFQRLQTLKINIFKFITDVKT------FFLHLLKTYTCFFFF 458 + L+C I+T + FF F + ++ D+KT FF H L + C FF Sbjct: 237 FFLSCTISTKYVGFFTFLSIGLSVCLELWYLWDIKTGLTVERFFQHFLARFFCLIFF 293 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 26.2 bits (55), Expect = 4.6 Identities = 9/15 (60%), Positives = 13/15 (86%) Frame = -1 Query: 540 KLISSNSLQTLKHFF 496 KL+SSN+LQ + HF+ Sbjct: 412 KLVSSNTLQAMSHFY 426 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,668,874 Number of Sequences: 5004 Number of extensions: 50759 Number of successful extensions: 149 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 131 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 327172622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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