BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00191 (758 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 5e-09 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 44 2e-05 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 41 2e-04 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 38 0.002 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.008 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 35 0.011 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 29 0.54 SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr... 25 8.9 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 25 8.9 SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c... 25 8.9 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 56.0 bits (129), Expect = 5e-09 Identities = 23/48 (47%), Positives = 33/48 (68%) Frame = +2 Query: 65 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDS 208 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + SD+ Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDA 50 Score = 32.7 bits (71), Expect = 0.058 Identities = 12/26 (46%), Positives = 19/26 (73%) Frame = +1 Query: 256 EYNINSMPTFVFVKNGKKLDEFSGAN 333 E +++MP+F KNG+K++E GAN Sbjct: 66 EAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 44.4 bits (100), Expect = 2e-05 Identities = 17/44 (38%), Positives = 29/44 (65%) Frame = +2 Query: 65 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 196 ++ D TR++ DK+ V+DF A WCGPCK + P L++++ + Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKLSEQ 63 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 40.7 bits (91), Expect = 2e-04 Identities = 19/63 (30%), Positives = 33/63 (52%) Frame = +2 Query: 17 VILFLVSIYLPKMSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAE 196 VI L ++ + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A Sbjct: 9 VIFALFALVFASGVVELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGAL 67 Query: 197 MSD 205 D Sbjct: 68 FED 70 Score = 30.3 bits (65), Expect = 0.31 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +2 Query: 113 KLVVIDFMATWCGPCKMIGPKLDEI 187 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 37.9 bits (84), Expect = 0.002 Identities = 14/31 (45%), Positives = 21/31 (67%) Frame = +2 Query: 113 KLVVIDFMATWCGPCKMIGPKLDEIAAEMSD 205 K V+++F A WCG CK + P +++A E SD Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSD 404 Score = 36.3 bits (80), Expect = 0.005 Identities = 12/30 (40%), Positives = 20/30 (66%) Frame = +2 Query: 110 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM 199 DK++++ F A WCG CK + P+ + A E+ Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADEL 68 Score = 29.5 bits (63), Expect = 0.54 Identities = 11/24 (45%), Positives = 16/24 (66%) Frame = +1 Query: 256 EYNINSMPTFVFVKNGKKLDEFSG 327 EY+I PT KNGK++ ++SG Sbjct: 89 EYSIRGYPTLNVFKNGKQISQYSG 112 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 35.5 bits (78), Expect = 0.008 Identities = 13/23 (56%), Positives = 19/23 (82%) Frame = +1 Query: 265 INSMPTFVFVKNGKKLDEFSGAN 333 + +MPTFVF +NGK++D +GAN Sbjct: 72 VKAMPTFVFFENGKQIDMLTGAN 94 Score = 34.3 bits (75), Expect = 0.019 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +2 Query: 119 VVIDFMATWCGPCKMIGPKLDEIAAE 196 + +D A WCGPCK I P ++A++ Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASK 47 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 35.1 bits (77), Expect = 0.011 Identities = 12/53 (22%), Positives = 28/53 (52%) Frame = +2 Query: 53 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 211 MS+ I + + L +++++++F A W PCK + D+ A + +++ Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAV 53 Score = 27.5 bits (58), Expect = 2.2 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +1 Query: 259 YNINSMPTFVFVKNGKKLDEFSGAN 333 +++N++P FV + K L SGAN Sbjct: 69 FDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 29.5 bits (63), Expect = 0.54 Identities = 14/43 (32%), Positives = 23/43 (53%) Frame = +2 Query: 71 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEM 199 +S + + + G LVV F A WCG CK + P ++A+ + Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNL 77 >SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr 2|||Manual Length = 905 Score = 25.4 bits (53), Expect = 8.9 Identities = 15/47 (31%), Positives = 25/47 (53%) Frame = +2 Query: 71 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMSDSI 211 + D + RL EAG + +A CGPC + + D+IA+ ++I Sbjct: 431 ERDGITERLEEAGATV-----LANACGPCIGMWKRTDDIASGEPNAI 472 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 25.4 bits (53), Expect = 8.9 Identities = 12/36 (33%), Positives = 18/36 (50%) Frame = -2 Query: 499 NIFKFITDVKNIFSSFIKNVYMFFFF*FHLVSIGGC 392 N ++TD +NIF +F+ N + F L I C Sbjct: 76 NSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111 >SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 302 Score = 25.4 bits (53), Expect = 8.9 Identities = 10/22 (45%), Positives = 16/22 (72%) Frame = +3 Query: 429 KNMYTFLINEEKMFLTSVMNLK 494 +N Y +L+NE+ FLTS+ +K Sbjct: 262 RNHYIYLLNEQVDFLTSIEIMK 283 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,869,050 Number of Sequences: 5004 Number of extensions: 55300 Number of successful extensions: 164 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 150 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 164 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 363302114 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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