BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00189X (548 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|... 133 1e-32 SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schiz... 133 1e-32 SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schi... 133 1e-32 SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||M... 133 1e-32 SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosacch... 133 1e-32 SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like pro... 85 9e-18 SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosacc... 43 3e-05 SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pomb... 41 1e-04 SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyc... 40 2e-04 SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schi... 29 0.34 SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharom... 27 2.4 SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit ... 26 3.2 SPBC25H2.14 |mug16||UNC-50 family protein|Schizosaccharomyces po... 26 3.2 SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr... 26 3.2 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 26 3.2 SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 25 7.3 SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Sch... 25 7.3 SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces po... 25 9.7 SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomy... 25 9.7 SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyc... 25 9.7 SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|c... 25 9.7 >SPAC1805.12c |uep1|ubi2|ribosomal-ubiquitin fusion protein Ubi2|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 3 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 62 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 63 KESTLHLV 70 >SPAC11G7.04 |ubi1||ribosomal-ubiquitin fusion protein Ubi1|Schizosaccharomyces pombe|chr 1|||Manual Length = 128 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 3 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 62 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 63 KESTLHLV 70 >SPAC6G10.11c |ubi3||ribosomal ubiquitin fusion protein Ubi3|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 3 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 62 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 63 KESTLHLV 70 Score = 63.3 bits (147), Expect = 2e-11 Identities = 27/49 (55%), Positives = 35/49 (71%) Frame = +3 Query: 291 LTVLRFYKVDEHGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTM 437 L VL++YKV++ G + RLRREC CGA FMA +DR YCG+CH T+ Sbjct: 100 LAVLKYYKVEDDGSVKRLRRECPN--CGASTFMANHKDRLYCGRCHLTL 146 >SPBC337.08c |ubi4||ubiquitin|Schizosaccharomyces pombe|chr 2|||Manual Length = 382 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 3 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 62 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 63 KESTLHLV 70 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 79 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 138 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 139 KESTLHLV 146 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 155 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 214 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 215 KESTLHLV 222 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 231 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 290 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 291 KESTLHLV 298 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 307 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 366 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 367 KESTLHLV 374 >SPAC589.10c |||ribomal-ubiquitin fusion protein Ubi5|Schizosaccharomyces pombe|chr 1|||Manual Length = 150 Score = 133 bits (322), Expect = 1e-32 Identities = 64/68 (94%), Positives = 67/68 (98%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 IFVKTLTGKTITLEVE SDTI+NVK+KIQDKEGIPP+QQRLIFAGKQLEDGRTLSDYNIQ Sbjct: 3 IFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQ 62 Query: 181 KESTLHLV 204 KESTLHLV Sbjct: 63 KESTLHLV 70 Score = 63.3 bits (147), Expect = 2e-11 Identities = 27/49 (55%), Positives = 35/49 (71%) Frame = +3 Query: 291 LTVLRFYKVDEHGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTM 437 L VL++YKV++ G + RLRREC CGA FMA +DR YCG+CH T+ Sbjct: 100 LAVLKYYKVEDDGSVKRLRRECPN--CGASTFMANHKDRLYCGRCHLTL 146 >SPBC12D12.08c |ned8|nedd8, ubl1, SPBC24C6.01c|ubiquitin-like protein modifier Ned8|Schizosaccharomyces pombe|chr 2|||Manual Length = 78 Score = 84.6 bits (200), Expect = 9e-18 Identities = 35/68 (51%), Positives = 52/68 (76%) Frame = +1 Query: 1 IFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQ 180 I VKTLTGK I L+++P+D + +K ++++KEGIPP+QQRLI+AGKQ+ D + Y+++ Sbjct: 3 IKVKTLTGKEIELDIDPNDKVSRIKERVEEKEGIPPSQQRLIYAGKQMADDKNAESYHLE 62 Query: 181 KESTLHLV 204 S LHLV Sbjct: 63 GGSVLHLV 70 >SPAC6G9.08 |ubp6||ubiquitin C-terminal hydrolase Ubp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 467 Score = 43.2 bits (97), Expect = 3e-05 Identities = 20/58 (34%), Positives = 33/58 (56%) Frame = +1 Query: 22 GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTL 195 GK LE+EP++T +K ++ +PP +Q++I G QL+D L I+ +TL Sbjct: 10 GKKYDLEIEPNETGSTLKHQLYSLTQVPPERQKVIVKGGQLKDDVLLGSVGIKPNATL 67 >SPBC2D10.12 |rhp23||Rad23 homolog Rhp23|Schizosaccharomyces pombe|chr 2|||Manual Length = 368 Score = 40.7 bits (91), Expect = 1e-04 Identities = 17/54 (31%), Positives = 33/54 (61%) Frame = +1 Query: 25 KTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 186 K + +V I +K KIQ ++ +Q+LI++G+ L D +T+ +YNI+++ Sbjct: 12 KFVISDVSADTKISELKEKIQTQQNYEVERQKLIYSGRILADDKTVGEYNIKEQ 65 >SPAC26A3.16 |dph1|ucp5|UBA domain protein Dph1|Schizosaccharomyces pombe|chr 1|||Manual Length = 354 Score = 39.9 bits (89), Expect = 2e-04 Identities = 20/66 (30%), Positives = 34/66 (51%) Frame = +1 Query: 7 VKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKE 186 +K + + V+ ++ +K I I +QRLI+AG+ L+D +L Y IQ Sbjct: 8 IKAANDQKYAVTVDSESSVLALKEAIAPVADIEKERQRLIYAGRVLKDEESLKTYKIQDG 67 Query: 187 STLHLV 204 ++HLV Sbjct: 68 HSIHLV 73 >SPBC31E1.03 |hub1|ubl4|ubiquitin-like protein modifier Hub1|Schizosaccharomyces pombe|chr 2|||Manual Length = 73 Score = 29.5 bits (63), Expect = 0.34 Identities = 16/60 (26%), Positives = 28/60 (46%) Frame = +1 Query: 22 GKTITLEVEPSDTIENVKAKIQDKEGIPPNQQRLIFAGKQLEDGRTLSDYNIQKESTLHL 201 GK + ++ P DT+ + K + + G P + L +D TL+DY I +L + Sbjct: 11 GKKVRVKCMPDDTVGDFKKLVAAQTGTDPRRIVLKKWHSVFKDNITLADYEIHDGMSLEM 70 >SPBC1718.06 |msp1|mgm1|mitochondrial GTPase Msp1|Schizosaccharomyces pombe|chr 2|||Manual Length = 903 Score = 26.6 bits (56), Expect = 2.4 Identities = 10/29 (34%), Positives = 17/29 (58%) Frame = -2 Query: 184 PSGYCSPRGYVHLPAAFQQRSTSVDWEGF 98 PS + PRG V+ P+ ++R + + GF Sbjct: 17 PSSFLKPRGLVYRPSQIRRRVSLLSLSGF 45 >SPBC3B8.11 |rrn6||RNA polymerase I transcription factor subunit Rrn6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 868 Score = 26.2 bits (55), Expect = 3.2 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = -1 Query: 542 SDTVSRFYLKSTYTAVSSVLYRLEGTYL 459 S+ F LK T+T V+ ++ LEGT L Sbjct: 634 SELYDGFPLKGTFTNVAEIISHLEGTEL 661 >SPBC25H2.14 |mug16||UNC-50 family protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 235 Score = 26.2 bits (55), Expect = 3.2 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -1 Query: 527 RFYLKSTYTAVSSVLYRLEGTYLFVV 450 RF +KS T +SS Y+LE Y F V Sbjct: 112 RFLMKSNDTILSSTDYQLEWNYCFDV 137 >SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochromatin assembly Hrr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1015 Score = 26.2 bits (55), Expect = 3.2 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = -1 Query: 428 VTLPAVVTVLHHRHEHTGAALLAC 357 VTL A+ T+L + H H L+AC Sbjct: 402 VTLKAIETLLENTHSHVLPILVAC 425 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 26.2 bits (55), Expect = 3.2 Identities = 27/73 (36%), Positives = 33/73 (45%) Frame = +1 Query: 316 STSMVRSTVFGASAQASSAAPVCSWR*WRTVTTAGSVTARWSSKTTTNK*VPSSL*RTLE 495 STS V T A A+SAA + S + T S TA SS T SS+ T + Sbjct: 1100 STSSVEGTASSTIAAAASAATLSSDAASGSSTVTSSATASSSSSAATT--ADSSV--TTD 1155 Query: 496 TAVYVDFK*NLDT 534 T DF N+DT Sbjct: 1156 TPSN-DFNANVDT 1167 >SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1082 Score = 25.0 bits (52), Expect = 7.3 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +1 Query: 310 TRSTSMVRSTVFGASAQASSAAPVCS 387 T+S S VRST ++ Q +SA+P+ S Sbjct: 344 TQSFSTVRSTSSSSTDQLTSASPISS 369 >SPAC688.10 |rev3||DNA polymerase zeta catalytic subunit Rev3|Schizosaccharomyces pombe|chr 1|||Manual Length = 1480 Score = 25.0 bits (52), Expect = 7.3 Identities = 12/24 (50%), Positives = 15/24 (62%) Frame = +1 Query: 106 PNQQRLIFAGKQLEDGRTLSDYNI 177 PNQ AGK+L+ R L DY+I Sbjct: 476 PNQGDTRKAGKRLKYSRNLDDYHI 499 >SPAC17G6.03 |||phosphoprotein phosphatase|Schizosaccharomyces pombe|chr 1|||Manual Length = 635 Score = 24.6 bits (51), Expect = 9.7 Identities = 13/26 (50%), Positives = 15/26 (57%) Frame = -1 Query: 83 ILAFTFSMVSDGSTSRVMVLPVRVFT 6 ILAF F G+T V VLPV F+ Sbjct: 182 ILAFGFVNSFSGATDTVRVLPVSQFS 207 >SPBC1E8.02 |||ubiquitin family protein, unknown|Schizosaccharomyces pombe|chr 2|||Manual Length = 603 Score = 24.6 bits (51), Expect = 9.7 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +2 Query: 227 RNARRGITPPQEDQAQEEERQTNRP 301 R A+R + P E Q ++E+ TN P Sbjct: 543 RTAQRVVEIPNETQTEDEQDGTNTP 567 >SPAC29B12.06c |rcd1||RNA-binding protein Rcd1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 283 Score = 24.6 bits (51), Expect = 9.7 Identities = 13/54 (24%), Positives = 24/54 (44%), Gaps = 1/54 (1%) Frame = +3 Query: 297 VLRFY-KVDEHGKIHRLRRECTGEQCGAGVFMAVMEDRHYCGKCHSTMVFKDDD 455 V+R Y ++ ++ + R C E F V++D H KC + ++ D Sbjct: 224 VIRCYLRLSDNPRAREALRHCLPEPLRDATFAQVLKDDHNTKKCLAQLLINLSD 277 >SPCC338.16 |pof3||F-box protein Pof3|Schizosaccharomyces pombe|chr 3|||Manual Length = 577 Score = 24.6 bits (51), Expect = 9.7 Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 2/34 (5%) Frame = +1 Query: 4 FVKTLTGKTITLEVEPSDTIE--NVKAKIQDKEG 99 F LT T T+E EP+ TI+ ++A++ +K G Sbjct: 22 FEDALTFITKTIEQEPNPTIDLFELRAQVYEKSG 55 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,270,649 Number of Sequences: 5004 Number of extensions: 46165 Number of successful extensions: 134 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 132 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 227943826 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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