Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00182
(787 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g62580.1 68416.m07030 expressed protein 50 1e-06
At1g72100.1 68414.m08334 late embryogenesis abundant domain-cont... 42 4e-04
At1g22600.1 68414.m02822 hypothetical protein 38 0.008
At2g44190.1 68415.m05497 expressed protein contains Pfam profil... 32 0.50
At5g22000.3 68418.m02560 zinc finger (C3HC4-type RING finger) fa... 31 0.66
At5g22000.2 68418.m02559 zinc finger (C3HC4-type RING finger) fa... 31 0.66
At5g22000.1 68418.m02558 zinc finger (C3HC4-type RING finger) fa... 31 0.66
At5g45400.1 68418.m05579 replication protein, putative similar t... 31 1.1
At5g27630.1 68418.m03310 acyl-CoA binding family protein similar... 30 1.5
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 29 4.6
At1g64050.1 68414.m07255 expressed protein 29 4.6
At4g27460.1 68417.m03946 CBS domain-containing protein 28 6.1
>At3g62580.1 68416.m07030 expressed protein
Length = 213
Score = 50.4 bits (115), Expect = 1e-06
Identities = 19/38 (50%), Positives = 28/38 (73%)
Frame = +1
Query: 394 ATHLGAQIWMTLVSGIVLYFSLPRHEFGRVQTILFPIY 507
AT GA +W T + GI+++ +LPRH+FG +Q+ LFP Y
Sbjct: 50 ATAWGAALWATFIGGIIMFKNLPRHQFGNLQSKLFPAY 87
>At1g72100.1 68414.m08334 late embryogenesis abundant
domain-containing protein / LEA domain-containing
protein low similarity to embryogenic gene [Betula
pendula] GI:4539485; contains Pfam profile PF02987: Late
embryogenesis abundant protein
Length = 480
Score = 42.3 bits (95), Expect = 4e-04
Identities = 18/43 (41%), Positives = 27/43 (62%)
Frame = +1
Query: 391 VATHLGAQIWMTLVSGIVLYFSLPRHEFGRVQTILFPIYSRST 519
+A G +W+T VS VL L R +FG VQ+ L+P+Y ++T
Sbjct: 318 IAAAFGTSVWVTFVSSYVLASVLGRQQFGVVQSKLYPVYFKAT 360
>At1g22600.1 68414.m02822 hypothetical protein
Length = 385
Score = 37.9 bits (84), Expect = 0.008
Identities = 16/42 (38%), Positives = 26/42 (61%)
Frame = +1
Query: 391 VATHLGAQIWMTLVSGIVLYFSLPRHEFGRVQTILFPIYSRS 516
+A G +W+T VSG VL L +FG VQ+ ++P+Y ++
Sbjct: 201 IAAAYGMCVWVTFVSGYVLASVLGEQQFGVVQSKMYPVYFKA 242
>At2g44190.1 68415.m05497 expressed protein contains Pfam profile:
PF04484 family of unknown function (DUF566)
Length = 474
Score = 31.9 bits (69), Expect = 0.50
Identities = 21/62 (33%), Positives = 32/62 (51%)
Frame = -1
Query: 748 SSPNIRRPTSWPPPADSSICVLTCLARSMGRTSRSLT*ASILNTANSSASCIHEVFSNSV 569
S+P I P PPP+ V +R M S S + ++++SSA +H++ SNS
Sbjct: 13 STPAINAPAPVPPPSTRRPRVREVSSRFMSPISSSSS-----SSSSSSAGDLHQLTSNSP 67
Query: 568 RH 563
RH
Sbjct: 68 RH 69
>At5g22000.3 68418.m02560 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 375
Score = 31.5 bits (68), Expect = 0.66
Identities = 22/92 (23%), Positives = 42/92 (45%), Gaps = 3/92 (3%)
Frame = +2
Query: 32 CNMCTRDVTLEAPTPPKVSRTLERMK-LKHNITTDPVNEEKQREVEYQPDLLAVSTQYTK 208
C MC + ++L+ PT ++ +E+ + + N T + +++ L V +
Sbjct: 70 CPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAE 129
Query: 209 IAYDVFKQ--AVVRLQE*RHTL*RHGHRSRST 298
I + + A + RH + R GHRSRS+
Sbjct: 130 IEERIIQHLAAAAAMGRARHGVRREGHRSRSS 161
>At5g22000.2 68418.m02559 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 375
Score = 31.5 bits (68), Expect = 0.66
Identities = 22/92 (23%), Positives = 42/92 (45%), Gaps = 3/92 (3%)
Frame = +2
Query: 32 CNMCTRDVTLEAPTPPKVSRTLERMK-LKHNITTDPVNEEKQREVEYQPDLLAVSTQYTK 208
C MC + ++L+ PT ++ +E+ + + N T + +++ L V +
Sbjct: 70 CPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAE 129
Query: 209 IAYDVFKQ--AVVRLQE*RHTL*RHGHRSRST 298
I + + A + RH + R GHRSRS+
Sbjct: 130 IEERIIQHLAAAAAMGRARHGVRREGHRSRSS 161
>At5g22000.1 68418.m02558 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 375
Score = 31.5 bits (68), Expect = 0.66
Identities = 22/92 (23%), Positives = 42/92 (45%), Gaps = 3/92 (3%)
Frame = +2
Query: 32 CNMCTRDVTLEAPTPPKVSRTLERMK-LKHNITTDPVNEEKQREVEYQPDLLAVSTQYTK 208
C MC + ++L+ PT ++ +E+ + + N T + +++ L V +
Sbjct: 70 CPMCWQSISLKDPTSQELLEAVEQERNFRFNPTRNATIFRHPTLGDFELQHLPVGVDNAE 129
Query: 209 IAYDVFKQ--AVVRLQE*RHTL*RHGHRSRST 298
I + + A + RH + R GHRSRS+
Sbjct: 130 IEERIIQHLAAAAAMGRARHGVRREGHRSRSS 161
>At5g45400.1 68418.m05579 replication protein, putative similar to
replication protein A 70kDa [Oryza sativa (japonica
cultivar-group)] GI:13536993; contains InterPro entry
IPR004365: OB-fold nucleic acid binding domain, PF04057:
Replication factor-A protein 1, N-terminal domain
Length = 853
Score = 30.7 bits (66), Expect = 1.1
Identities = 23/86 (26%), Positives = 38/86 (44%)
Frame = -1
Query: 619 TANSSASCIHEVFSNSVRHCVRRYARSPSKLMQALNASR*GTGWSARGRTRVSEGRSIGR 440
+ANS + S++ R+CV + P + + +A R G ++ R V GR
Sbjct: 159 SANSPQPQVVHNSSDAGRYCVSANSPQPQVVHSSSDAGRYGVSANSPQRQVVHNSPDAGR 218
Query: 439 YRTQASSISELRGEWPRPPRTAPSSR 362
Y +S+ G P T+PS+R
Sbjct: 219 Y--GQPQVSQRYGTGSGYPETSPSTR 242
>At5g27630.1 68418.m03310 acyl-CoA binding family protein similar to
RING finger rngB protein, cytosolic - Dictyostelium
discoideum, PIR:S68824; contains Pfam profiles PF01344:
Kelch motif, PF00887: Acyl CoA binding protein (ACBP)
Length = 648
Score = 30.3 bits (65), Expect = 1.5
Identities = 15/66 (22%), Positives = 32/66 (48%)
Frame = +2
Query: 14 DVLNRMCNMCTRDVTLEAPTPPKVSRTLERMKLKHNITTDPVNEEKQREVEYQPDLLAVS 193
D + + N TRD+ E K R + +K + +N+EK + ++ + +L +
Sbjct: 502 DSFSAVNNATTRDIESEIKVEGKADRIITTLKSEKEEVEASLNKEKIQTLQLKEELAEID 561
Query: 194 TQYTKI 211
T+ T++
Sbjct: 562 TRNTEL 567
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 28.7 bits (61), Expect = 4.6
Identities = 20/76 (26%), Positives = 38/76 (50%), Gaps = 7/76 (9%)
Frame = +2
Query: 47 RDVTLEAPTPPKVS----RTLERMKLKHNITTDP---VNEEKQREVEYQPDLLAVSTQYT 205
+D +E P P +VS + ER+K + ++ + V EE++RE E + D +A +
Sbjct: 1115 QDAKVERPLPSRVSVQREKEAERLKRERDLEMEQLRKVEEEREREREREKDRMAFDQRAL 1174
Query: 206 KIAYDVFKQAVVRLQE 253
A + ++A +E
Sbjct: 1175 ADARERLEKACAEARE 1190
>At1g64050.1 68414.m07255 expressed protein
Length = 668
Score = 28.7 bits (61), Expect = 4.6
Identities = 18/59 (30%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
Frame = -1
Query: 727 PTSWPPPADSSICVLTCLARSMGRTSRSLT*ASILNTAN-SSASCIH-EVFSNSVRHCV 557
PT P + SS + TCL+R++G S++ A++ N+ N + + ++ S+S HC+
Sbjct: 269 PTKRPVQSQSSTELDTCLSRTLGSPPSSIS-ATLPNSENIDKVNALDGDLSSSSKDHCI 326
>At4g27460.1 68417.m03946 CBS domain-containing protein
Length = 391
Score = 28.3 bits (60), Expect = 6.1
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +2
Query: 485 RPSCSLSTRVQRLHELAGTPGVPPHTVPYGIREHLMDTASTTVSS 619
+PSCSL + + + A VP HT PY ++ D S T ++
Sbjct: 105 QPSCSLIEAIDLIIKGAQNLIVPIHTKPYTKKKQHNDNVSVTTTT 149
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,769,427
Number of Sequences: 28952
Number of extensions: 329936
Number of successful extensions: 974
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 934
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 974
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1765546400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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