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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00180
         (595 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)                50   1e-06
SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)              39   0.003
SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)              37   0.011
SB_47462| Best HMM Match : Ras (HMM E-Value=0)                         37   0.011
SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)            37   0.014
SB_14527| Best HMM Match : SAP (HMM E-Value=1.6e-13)                   35   0.057
SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)              34   0.099
SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)               33   0.13 
SB_23555| Best HMM Match : No HMM Matches (HMM E-Value=.)              31   0.70 
SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)              30   1.6  
SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)                29   2.1  
SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)             29   2.1  
SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)                 29   2.8  
SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)      29   2.8  
SB_4913| Best HMM Match : CBF (HMM E-Value=2.9)                        28   4.9  
SB_32709| Best HMM Match : Spectrin (HMM E-Value=8.8)                  28   4.9  
SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)          28   6.5  
SB_42415| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   8.6  
SB_9139| Best HMM Match : No HMM Matches (HMM E-Value=.)               27   8.6  
SB_54514| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   8.6  
SB_38755| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   8.6  

>SB_41644| Best HMM Match : Tetraspannin (HMM E-Value=0)
          Length = 252

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 24/83 (28%), Positives = 38/83 (45%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           FL CCGA++ES CM+ TY                  + Y E I++++   +     +   
Sbjct: 74  FLACCGALKESQCMLGTYFGLVTAIFCLEVTAIVLAYVYRERIEKNLHGDIRSTMNEYHL 133

Query: 182 ANADEAAEAVFSELQRQFECCGN 250
           +  D    A+  E+ R F+CCGN
Sbjct: 134 SGHDAVTRAI-DEIHRDFKCCGN 155



 Score = 29.9 bits (64), Expect = 1.6
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 3/75 (4%)
 Frame = +1

Query: 280 LPESCCVKKSILSTFAGNNCTVDAAN---PGCGPKIGELYQKWNKPIAGVAIGVACVEVV 450
           +P SCC   S   +    N + D       GC   + +        I+G  I +A ++++
Sbjct: 174 VPRSCCKDPS--DSKCNENISKDPTKIYRRGCYMFVKKYLSNNLHVISGFGIWIAVIQLM 231

Query: 451 GALFALCLANSIRNM 495
           G +FA CL   IR +
Sbjct: 232 GIIFATCLCCHIRTI 246


>SB_23554| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 514

 Score = 38.7 bits (86), Expect = 0.003
 Identities = 17/82 (20%), Positives = 34/82 (41%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           F GCCGAI+E  C++ T+                  + Y   ++E +   +        +
Sbjct: 341 FFGCCGAIKEHRCLLATFFALLFIILAVEITGTALGYVYRNKVEEQLGKDMNDTIVSYGE 400

Query: 182 ANADEAAEAVFSELQRQFECCG 247
              +   +A +  +Q++ +CCG
Sbjct: 401 KGKEGITDA-YDAIQKKEKCCG 421



 Score = 33.1 bits (72), Expect = 0.17
 Identities = 20/69 (28%), Positives = 33/69 (47%)
 Frame = +1

Query: 280 LPESCCVKKSILSTFAGNNCTVDAANPGCGPKIGELYQKWNKPIAGVAIGVACVEVVGAL 459
           +P+SCC  K++      N    D    GC   +  L +     I G+ IGVA ++++  +
Sbjct: 441 VPDSCC--KTVSKGCGINFKENDIYTKGCFDTVKTLLKDNLIIIGGIGIGVAVIQILAMI 498

Query: 460 FALCLANSI 486
           FA+ L   I
Sbjct: 499 FAMVLVCKI 507


>SB_15887| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 333

 Score = 37.1 bits (82), Expect = 0.011
 Identities = 20/82 (24%), Positives = 39/82 (47%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           F GC GA+RE+ C++  ++                 F + + ++  I++ +     K  D
Sbjct: 62  FSGCIGALRENICLLKFFSVVLAIIFFLQLALGVFVFVFQDKVEAVIVEKLQTAVTKYRD 121

Query: 182 ANADEAAEAVFSELQRQFECCG 247
            NAD   + +   +Q++F+CCG
Sbjct: 122 -NAD--LQNLIDGVQQEFKCCG 140


>SB_47462| Best HMM Match : Ras (HMM E-Value=0)
          Length = 385

 Score = 37.1 bits (82), Expect = 0.011
 Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 2/74 (2%)
 Frame = +1

Query: 277 TLPESCCVKKSILSTFAGNNCTVDAANP--GCGPKIGELYQKWNKPIAGVAIGVACVEVV 450
           ++PESCC    + S         +  N   GC     + ++     I GVA+G+A  +++
Sbjct: 305 SVPESCCKDTKVKSCNLEVLSHPETINSEQGCYGAAVKYFEDKLVIIGGVALGLAVFQLI 364

Query: 451 GALFALCLANSIRN 492
           G  F+ CLA+++ N
Sbjct: 365 GIAFSCCLASTLHN 378



 Score = 29.5 bits (63), Expect = 2.1
 Identities = 9/17 (52%), Positives = 14/17 (82%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVT 52
           F+GCCGA++E  C++ T
Sbjct: 232 FIGCCGALKEHTCLLKT 248


>SB_46721| Best HMM Match : Tetraspannin (HMM E-Value=4e-35)
          Length = 642

 Score = 36.7 bits (81), Expect = 0.014
 Identities = 20/82 (24%), Positives = 33/82 (40%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           F GCCGA +E+ CM++ +                  +   + I+  + D +    K+   
Sbjct: 422 FFGCCGAWKENKCMLILFFILLLIVLGLEIAAAVLGYINKDKIQSDLTDDIIRNIKEYPK 481

Query: 182 ANADEAAEAVFSELQRQFECCG 247
            N     +A    +Q  FECCG
Sbjct: 482 NN-----KAAIDAMQMDFECCG 498


>SB_14527| Best HMM Match : SAP (HMM E-Value=1.6e-13)
          Length = 347

 Score = 34.7 bits (76), Expect = 0.057
 Identities = 16/42 (38%), Positives = 22/42 (52%)
 Frame = -1

Query: 127 ALAVGEEEDADHHLHNDDHQQKDCVRDDHAVTLAYRSTASQE 2
           AL  GE+ + D  L NDDH + D + DD    L    + S+E
Sbjct: 47  ALHEGEDVEGDDDLDNDDHDEDDVLADDSVDDLVAEESLSEE 88


>SB_58401| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 426

 Score = 33.9 bits (74), Expect = 0.099
 Identities = 11/17 (64%), Positives = 14/17 (82%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVT 52
           F GCCGA++ES CM+ T
Sbjct: 329 FFGCCGAVKESRCMLAT 345


>SB_1751| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 341

 Score = 33.5 bits (73), Expect = 0.13
 Identities = 18/82 (21%), Positives = 35/82 (42%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           FLGCCGA +E+ CM++ +                  +   + I+  +   +      ++ 
Sbjct: 69  FLGCCGAWKENKCMLICFFAFLLLLLILEIVGGALAYNNKDKIENRLDKDI-----LKAI 123

Query: 182 ANADEAAEAVFSELQRQFECCG 247
            N     E   +++Q +F+CCG
Sbjct: 124 ENYPGKNEKSINDMQTKFKCCG 145



 Score = 31.9 bits (69), Expect = 0.40
 Identities = 18/57 (31%), Positives = 26/57 (45%)
 Frame = +1

Query: 277 TLPESCCVKKSILSTFAGNNCTVDAANPGCGPKIGELYQKWNKPIAGVAIGVACVEV 447
           ++P+SCC  +       G     D    GC   I    +K  KPI G+AI V  ++V
Sbjct: 163 SVPDSCCKSEKAGCGVGGVKDPKDIYTKGCFTIIKGEIEKSLKPIGGLAIAVLVIQV 219


>SB_23555| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 140

 Score = 31.1 bits (67), Expect = 0.70
 Identities = 10/15 (66%), Positives = 13/15 (86%)
 Frame = +2

Query: 2  FLGCCGAIRESHCMV 46
          F GCCGAI+ES C++
Sbjct: 41 FFGCCGAIKESRCLL 55


>SB_22250| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 313

 Score = 29.9 bits (64), Expect = 1.6
 Identities = 17/86 (19%), Positives = 32/86 (37%), Gaps = 1/86 (1%)
 Frame = +2

Query: 8   GCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGV-GVLFKKRSDA 184
           GC GA+RE+ C++  Y                  F Y + ++  +   +   +   R   
Sbjct: 121 GCIGALRENRCLLTAYIWMCGIILAGMIVCGGLGFYYKDVLETKVTAQLKDAIVLYRDPT 180

Query: 185 NADEAAEAVFSELQRQFECCGNTALS 262
             D     +   +Q + +CCG   L+
Sbjct: 181 KGD--LHLIIDTVQTELQCCGVQGLN 204


>SB_31428| Best HMM Match : Tetraspannin (HMM E-Value=0)
          Length = 280

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 18/82 (21%), Positives = 33/82 (40%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           F GC GA+RE+ C +  Y                  +T  + + +++ + +    KK  D
Sbjct: 93  FFGCVGALRENTCFLTVYGTSVFLCLILLIACGAVAYTQKDKV-QALKERLKQDLKKYLD 151

Query: 182 ANADEAAEAVFSELQRQFECCG 247
              D   + +   +Q   +CCG
Sbjct: 152 ---DPLNQDLIDFMQTLLKCCG 170


>SB_2984| Best HMM Match : Tetraspannin (HMM E-Value=9e-06)
          Length = 187

 Score = 29.5 bits (63), Expect = 2.1
 Identities = 9/17 (52%), Positives = 14/17 (82%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVT 52
           F+GCCGA++E  C++ T
Sbjct: 90  FIGCCGALKEHTCLLKT 106


>SB_47590| Best HMM Match : TSP_3 (HMM E-Value=7.6e-09)
          Length = 669

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 8/16 (50%), Positives = 14/16 (87%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVV 49
           F+GCCGA++E+ C ++
Sbjct: 214 FVGCCGAVKENKCFLL 229


>SB_26670| Best HMM Match : Tetraspannin (HMM E-Value=1.09301e-43)
          Length = 211

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 20/91 (21%), Positives = 35/91 (38%), Gaps = 9/91 (9%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTY-GES--------IKESIMDGV 154
           F GC GA +E+ CM+ T+                  + Y G++        +K+ I   +
Sbjct: 71  FFGCLGAWKENRCMLGTFFVLLLILLTLEIAAAVLAYNYRGKNSLNSDSVKVKDEIESDL 130

Query: 155 GVLFKKRSDANADEAAEAVFSELQRQFECCG 247
               K    ++  +     F  LQ + +CCG
Sbjct: 131 TKALKGDYGSSGQDGVTKAFDALQEKQKCCG 161


>SB_4913| Best HMM Match : CBF (HMM E-Value=2.9)
          Length = 328

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 143 MDGVGVLFKKRSDANADEAAEAVFSELQR 229
           + G+G++FK+R+  N       +F ELQR
Sbjct: 55  LKGLGLMFKQRTGRNLPMTHPVIFDELQR 83


>SB_32709| Best HMM Match : Spectrin (HMM E-Value=8.8)
          Length = 230

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 11/29 (37%), Positives = 18/29 (62%)
 Frame = +2

Query: 143 MDGVGVLFKKRSDANADEAAEAVFSELQR 229
           + G+G++FK+R+  N       +F ELQR
Sbjct: 55  LKGLGLMFKQRTGRNLPMTHPVIFDELQR 83


>SB_31429| Best HMM Match : Tetraspannin (HMM E-Value=5.9e-35)
          Length = 286

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 17/82 (20%), Positives = 30/82 (36%)
 Frame = +2

Query: 2   FLGCCGAIRESHCMVVTYAXXXXXXXXXXXXXXXXXFTYGESIKESIMDGVGVLFKKRSD 181
           F GC GA++E+   + +Y                  F      +E ++D    L      
Sbjct: 88  FFGCVGALKENTLFLASYGIFIFCCLIPIIAAGVLAFLQ----REKLVDPKARLEDGLKS 143

Query: 182 ANADEAAEAVFSELQRQFECCG 247
              D + +     +Q +F+CCG
Sbjct: 144 YQQDSSKQEFIDFIQTRFKCCG 165


>SB_42415| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 806

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 10/25 (40%), Positives = 13/25 (52%), Gaps = 1/25 (4%)
 Frame = +1

Query: 328 GNNCTVDAANPGCGPKI-GELYQKW 399
           G  C ++  NPGCG K   +   KW
Sbjct: 535 GGRCPIECQNPGCGIKFCSDCEHKW 559


>SB_9139| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 157

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 11/19 (57%), Positives = 17/19 (89%)
 Frame = +2

Query: 539 TPKMKTSRDEHSNKKKKKK 595
           TPK +TS++ ++NKK+KKK
Sbjct: 88  TPKNETSQNLNNNKKEKKK 106


>SB_54514| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 189

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +1

Query: 364 CGPKIGELYQKWNKPIAGVAIGVACVEVVGALFA 465
           C  K+ +L Q+ +KP   + +GV C E + A+ A
Sbjct: 81  CFIKVVQLVQQASKPQEAIFVGVGCFESMTAVLA 114


>SB_38755| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 471

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 115 GEEEDADHHLHNDDHQQKDCVRDDHAVTLA 26
           G+++DAD    NDD  + D V DD  + LA
Sbjct: 122 GDDDDADDDNGNDDEDKSD-VDDDDVIPLA 150


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,612,823
Number of Sequences: 59808
Number of extensions: 317701
Number of successful extensions: 1090
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 934
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1085
length of database: 16,821,457
effective HSP length: 79
effective length of database: 12,096,625
effective search space used: 1427401750
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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