Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00176
(788 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 125 6e-30
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 28 1.3
SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 2.3
SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 27 3.1
SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 5.4
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 26 7.1
SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 25 9.4
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 9.4
>SPAC926.04c |hsp90|swo1|heat shock protein
Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
Length = 704
Score = 125 bits (302), Expect = 6e-30
Identities = 59/85 (69%), Positives = 71/85 (83%)
Frame = +2
Query: 254 TLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEAL 433
+L+DP LD+ K+L+I+I P+K L+I DTGIGMTK DL+NNLG IAKSGTK FMEA
Sbjct: 50 SLSDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAA 109
Query: 434 QAGADISMIGQFGVGFYSSYLVADR 508
+GADISMIGQFGVGFYS+YLVAD+
Sbjct: 110 ASGADISMIGQFGVGFYSAYLVADK 134
Score = 86.6 bits (205), Expect = 4e-18
Identities = 44/84 (52%), Positives = 57/84 (67%), Gaps = 1/84 (1%)
Frame = +1
Query: 514 VHSKHNDDEQYVWESSAGGSFTVRPDSGEP-LGRGTKIVLHVKEDLAEFMXXXXXXXXXX 690
V SKHNDDEQY+WESSAGGSFTV D+ P L RGT+I L +KED +++
Sbjct: 137 VVSKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVK 196
Query: 691 XXSQFIGYPIKLMVEKEREKELSD 762
S+FI YPI+L+V +E EKE+ +
Sbjct: 197 KHSEFISYPIQLVVTREVEKEVPE 220
Score = 82.6 bits (195), Expect = 6e-17
Identities = 39/48 (81%), Positives = 44/48 (91%)
Frame = +3
Query: 111 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYE 254
+ ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIRY+
Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQ 49
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 28.3 bits (60), Expect = 1.3
Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 5/66 (7%)
Frame = +3
Query: 183 SNKEIFLRELISNSSDALDKIRYELSRIRQNSI-VAKSCTSRSFPTRTRALLRSSI---- 347
S+ +L +L+++ D L K+R E RIR+ I V S T TRA+++ +
Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRP 384
Query: 348 PVLV*P 365
PVL+ P
Sbjct: 385 PVLMVP 390
>SPAC3H1.04c |mdm31||mitochondrial inner membrane protein
Mdm31|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 27.5 bits (58), Expect = 2.3
Identities = 20/53 (37%), Positives = 30/53 (56%)
Frame = -3
Query: 420 KALVPDFAMVPKLFTKSALVIPIPVSMIVRVPSFLLGMILMYSSLPLSSFDGS 262
KA++P F + +AL+ PI ++ I +F+ IL + S PLS FDGS
Sbjct: 486 KAVIPIFTNQVS-YINNALIRPI-IAYINSTRTFI--PILCHVSKPLSDFDGS 534
>SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 376
Score = 27.1 bits (57), Expect = 3.1
Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +1
Query: 241 KSGMNSHGSVKTR*WQRAVHQDHSQ-QERGHSYDHRYRYWYDQGRFGEQF 387
+S H S K +R+ D + +ER H+ +R+R YD G F + +
Sbjct: 187 RSDNEQHSSDKREHSRRSYRNDRNNWRERTHNDRYRHRDKYDSGYFKKHY 236
>SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 26.2 bits (55), Expect = 5.4
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = -2
Query: 397 DGSQIVHQIGLGHTNTGIDDRKSALVLVGNDLDVQL 290
DG V I G +DDR+ L+ + NDL+V L
Sbjct: 2 DGKPQVEVIVNGQVVPNLDDREYRLIKLENDLEVLL 37
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 25.8 bits (54), Expect = 7.1
Identities = 14/64 (21%), Positives = 32/64 (50%)
Frame = +3
Query: 84 MPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYELSR 263
+ E++ + A+ E+F A+L +N NKE + +L + S+ +++ S+
Sbjct: 762 LTEKLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQ 821
Query: 264 IRQN 275
+ Q+
Sbjct: 822 LNQD 825
>SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 817
Score = 25.4 bits (53), Expect = 9.4
Identities = 8/29 (27%), Positives = 16/29 (55%)
Frame = +1
Query: 301 QDHSQQERGHSYDHRYRYWYDQGRFGEQF 387
+DH++++ H+ +Y YD R E +
Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIWENY 631
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 25.4 bits (53), Expect = 9.4
Identities = 12/43 (27%), Positives = 22/43 (51%)
Frame = +2
Query: 263 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLG 391
DPSK ++Y K+ + + L D+GI ++ L+ +G
Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIG 2514
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,252,555
Number of Sequences: 5004
Number of extensions: 70410
Number of successful extensions: 210
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 200
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 383374054
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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