Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00175
(682 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondr... 180 4e-44
UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Ma... 151 2e-35
UniRef50_A0L5P5 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 146 4e-34
UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|R... 136 4e-31
UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187; cell... 136 4e-31
UniRef50_Q0AIF8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 134 2e-30
UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella ve... 130 4e-29
UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52; cell... 129 7e-29
UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,... 126 7e-28
UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63; Euka... 126 7e-28
UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplas... 120 3e-26
UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Mali... 120 4e-26
UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole geno... 118 1e-25
UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondri... 118 2e-25
UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 117 2e-25
UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon nigrovirid... 115 9e-25
UniRef50_A7IMB8 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 113 3e-24
UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:... 110 3e-23
UniRef50_A7CWP9 Cluster: Malate dehydrogenase (Oxaloacetate-deca... 109 5e-23
UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2; Ostre... 107 2e-22
UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20; Trypanoso... 106 4e-22
UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila melanogas... 105 8e-22
UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor circinelloides... 105 8e-22
UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep: ... 105 1e-21
UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;... 104 2e-21
UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;... 104 2e-21
UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic... 103 3e-21
UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 103 3e-21
UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2; Ostr... 103 4e-21
UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Ma... 100 3e-20
UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus californi... 100 4e-20
UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep... 100 4e-20
UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isofo... 99 5e-20
UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 99 1e-19
UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 96 8e-19
UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:... 94 2e-18
UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 94 3e-18
UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase (... 93 4e-18
UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2; Mucoromyco... 91 2e-17
UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2; Aeromo... 90 4e-17
UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:... 89 7e-17
UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon nigrovirid... 87 3e-16
UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2; ... 87 3e-16
UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n... 86 9e-16
UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6; Deinococci|... 84 3e-15
UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme; ... 83 5e-15
UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:... 83 6e-15
UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella neofo... 82 1e-14
UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep: ... 81 2e-14
UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Ma... 81 3e-14
UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10; Franc... 80 4e-14
UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium globosum|... 78 2e-13
UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|R... 77 4e-13
UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia intestinalis... 77 5e-13
UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep: ... 77 5e-13
UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Mali... 73 9e-12
UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondri... 71 3e-11
UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococc... 70 6e-11
UniRef50_Q5C370 Cluster: Malic enzyme; n=1; Schistosoma japonicu... 66 7e-10
UniRef50_Q27113 Cluster: Malic enzyme; n=1; Trichomonas vaginali... 62 1e-08
UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3; Schizo... 62 1e-08
UniRef50_A7R628 Cluster: Chromosome undetermined scaffold_1141, ... 54 2e-06
UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15... 54 4e-06
UniRef50_A5B3Q7 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 49 1e-04
UniRef50_Q8U225 Cluster: Malate oxidoreductase; n=41; cellular o... 43 0.008
UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n... 41 0.024
UniRef50_Q3W7J3 Cluster: Malate dehydrogenase; n=1; Frankia sp. ... 41 0.032
UniRef50_A5C5G3 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep: ... 39 0.098
UniRef50_Q5Y0M3 Cluster: Malate oxidoreductase; n=8; cellular or... 39 0.098
UniRef50_P54572 Cluster: Probable NAD-dependent malic enzyme 1; ... 39 0.098
UniRef50_A0DE35 Cluster: Malic enzyme; n=3; Paramecium tetraurel... 38 0.17
UniRef50_Q4SN44 Cluster: Chromosome 8 SCAF14543, whole genome sh... 37 0.40
UniRef50_Q8R7R6 Cluster: Malic enzyme; n=10; cellular organisms|... 37 0.52
UniRef50_Q2KKD0 Cluster: Oxalacetate decarboxylase; n=11; Lactob... 36 0.69
UniRef50_Q73UK5 Cluster: Putative uncharacterized protein; n=1; ... 36 0.91
UniRef50_Q9HKY7 Cluster: Malate oxidoreductase (Malic enzyme) re... 36 0.91
UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putativ... 36 1.2
UniRef50_UPI00003ABEA3 Cluster: PREDICTED: hypothetical protein;... 35 2.1
UniRef50_Q1GLN4 Cluster: Putative uncharacterized protein; n=1; ... 35 2.1
UniRef50_Q8PVS2 Cluster: Conserved protein; n=3; Methanosarcina|... 35 2.1
UniRef50_Q580K5 Cluster: Putative uncharacterized protein; n=3; ... 34 2.8
UniRef50_Q7URP6 Cluster: Putative uncharacterized protein; n=1; ... 34 3.7
UniRef50_Q3I797 Cluster: Putative uncharacterized protein; n=10;... 33 4.9
UniRef50_UPI00015555BF Cluster: PREDICTED: similar to leishmanol... 33 6.4
UniRef50_Q47IV3 Cluster: Putative uncharacterized protein; n=1; ... 33 8.5
UniRef50_Q08WN0 Cluster: Putative uncharacterized protein; n=1; ... 33 8.5
UniRef50_A7CYR8 Cluster: D-isomer specific 2-hydroxyacid dehydro... 33 8.5
UniRef50_A0T6Y3 Cluster: Putative uncharacterized protein; n=1; ... 33 8.5
>UniRef50_Q16798 Cluster: NADP-dependent malic enzyme, mitochondrial
precursor; n=15; Bilateria|Rep: NADP-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 604
Score = 180 bits (437), Expect = 4e-44
Identities = 104/222 (46%), Positives = 133/222 (59%), Gaps = 7/222 (3%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
++ L+KYI LM L DRNE LFYR + +V + MPIVYTPTVGLACQ +GL +RRPRGLFI
Sbjct: 101 QSDLDKYIILMTLQDRNEKLFYRVLTSDVEKFMPIVYTPTVGLACQHYGLTFRRPRGLFI 160
Query: 218 TIHDKGHVYDVLRT-GRRRTSAPLL*PTENV-----FWAWAISARAGWASLW-ANSRSTP 376
TIHDKGH+ +L + A ++ E + + + G +L+ A P
Sbjct: 161 TIHDKGHLATMLNSWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNP 220
Query: 377 RSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVVRRYGQN 556
+ L+ V + +R P G + L LDEFM+AV ++G N
Sbjct: 221 QQCLPVLLDVGTNNEELLRDPLYIGLKH---QRVHGKAYDDL---LDEFMQAVTDKFGIN 274
Query: 557 CLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
CL+QFEDF NANAFRLL KY NKYC FNDDIQGTA+VAVAG+
Sbjct: 275 CLIQFEDFANANAFRLLNKYRNKYCMFNDDIQGTASVAVAGI 316
Score = 124 bits (300), Expect = 2e-27
Identities = 53/78 (67%), Positives = 65/78 (83%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
+WPE +++A+VVTDGERILGLGD G GMGIPVGKLALYTA GG+ P QCLP+ +DVGT
Sbjct: 174 SWPEDNIKAVVVTDGERILGLGDLGCYGMGIPVGKLALYTACGGVNPQQCLPVLLDVGTN 233
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L DPLYIGL+ +RV
Sbjct: 234 NEELLRDPLYIGLKHQRV 251
>UniRef50_A2ZQ54 Cluster: Malic enzyme; n=9; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 613
Score = 151 bits (366), Expect = 2e-35
Identities = 83/228 (36%), Positives = 122/228 (53%), Gaps = 1/228 (0%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ ++ Y PL +Y+ +M L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+
Sbjct: 139 QVKKIMHNLRQYSVPLQRYMAMMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKY 198
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRT-GRRRTSAPLL*PTENVFWAWAISARAGWASLWA 358
G ++R+P+GL++++ DKG V DVLR R ++ E + + + +
Sbjct: 199 GSIFRQPQGLYVSLKDKGKVLDVLRNWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGK 258
Query: 359 NSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVV 538
S T + ++ + A L++EFM AV
Sbjct: 259 LSLYTALGGVRPSACLPITIDVGTNNEQLLNDEFYIGLRQRRATGKEYHELMEEFMSAVK 318
Query: 539 RRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ YG+ L+QFEDF N NAF LL KY + FNDDIQGTA+V +AGL
Sbjct: 319 QIYGEKVLIQFEDFANHNAFDLLAKYSKSHLVFNDDIQGTASVVLAGL 366
Score = 126 bits (305), Expect = 4e-28
Identities = 56/77 (72%), Positives = 65/77 (84%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPE +++ IVVTDGERILGLGD G GMGIPVGKL+LYTALGG++P CLPITIDVGT
Sbjct: 224 NWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPITIDVGTN 283
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+D YIGLRQRR
Sbjct: 284 NEQLLNDEFYIGLRQRR 300
>UniRef50_A0L5P5 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Magnetococcus sp. MC-1|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Magnetococcus
sp. (strain MC-1)
Length = 556
Score = 146 bits (354), Expect = 4e-34
Identities = 92/229 (40%), Positives = 131/229 (57%), Gaps = 15/229 (6%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
N L KYI+L GL +RNE LFYR V N+ EM+PI+YTPTVG ACQ +G ++RRP+G+FI+
Sbjct: 71 NDLEKYIFLTGLQERNETLFYRLVMTNIEEMLPIIYTPTVGKACQTYGHIFRRPQGMFIS 130
Query: 221 IHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWANSRSTPRSEASSLI 400
I+DKG + ++L + ++ T+ S G L A+ P + +
Sbjct: 131 INDKGRIAELLGNWVHK-DVRVIVVTDG-------SRILGLGDLGAHGMGIPVGK----L 178
Query: 401 SVYPSLSTWVRSPSRCWTTRCTSGSGS----------GACWPRLR-RLLDE----FMRAV 535
++Y +L+ P C G+ + G PR+ + DE F+ AV
Sbjct: 179 ALYTALAG--IPPIHCLPVTLDMGTNNEALRNDPLYVGLPQPRVTGEIYDEMIEEFVLAV 236
Query: 536 VRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++ ++QFEDF N NAFRLLEKY ++ CTFNDDIQGTAAVA+AG+
Sbjct: 237 QEQF-PRAMIQFEDFSNDNAFRLLEKYQDRICTFNDDIQGTAAVALAGI 284
Score = 118 bits (284), Expect = 1e-25
Identities = 54/78 (69%), Positives = 62/78 (79%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NW DVR IVVTDG RILGLGD GA GMGIPVGKLALYTAL GI P CLP+T+D+GT
Sbjct: 143 NWVHKDVRVIVVTDGSRILGLGDLGAHGMGIPVGKLALYTALAGIPPIHCLPVTLDMGTN 202
Query: 436 TQSMLDDPLYIGLRQRRV 489
+++ +DPLY+GL Q RV
Sbjct: 203 NEALRNDPLYVGLPQPRV 220
>UniRef50_Q89G76 Cluster: Malic enzyme; n=3; cellular organisms|Rep:
Malic enzyme - Bradyrhizobium japonicum
Length = 531
Score = 136 bits (330), Expect = 4e-31
Identities = 91/241 (37%), Positives = 126/241 (52%), Gaps = 14/241 (5%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ ++ L KY+ L L DRNE LF+R V DN+ E+ PI+YTPTVGLACQK+
Sbjct: 39 QVDRVLTNLRMLPTDLEKYVALNALHDRNEALFFRVVVDNIDEIQPIIYTPTVGLACQKY 98
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWAN 361
GL+++RPRG+FI+ D+G + ++L+ ++ E + G L AN
Sbjct: 99 GLIFQRPRGMFISSRDRGQIAEILKNWPYPARLIVVTDGERIL---------GLGDLGAN 149
Query: 362 SRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWP-------RLRRL--- 511
P + +S+Y S V P C G+ + R RRL
Sbjct: 150 GMGIPVGK----LSLY-SACAGVH-PEHCLPIVLDVGTNNEELLNDPYYLGLRERRLTGE 203
Query: 512 -LDEFM---RAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
D F+ R+ L+QFEDF N +AF+LL KY ++ C FNDDIQGTAAVA+AG
Sbjct: 204 AYDSFVDEFMVAARKTFPGVLIQFEDFANHSAFKLLHKYRDEACVFNDDIQGTAAVALAG 263
Query: 680 L 682
L
Sbjct: 264 L 264
Score = 112 bits (270), Expect = 7e-24
Identities = 51/78 (65%), Positives = 61/78 (78%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP R IVVTDGERILGLGD GA GMGIPVGKL+LY+A G+ P CLPI +DVGT
Sbjct: 124 NWPYP-ARLIVVTDGERILGLGDLGANGMGIPVGKLSLYSACAGVHPEHCLPIVLDVGTN 182
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L+DP Y+GLR+RR+
Sbjct: 183 NEELLNDPYYLGLRERRL 200
>UniRef50_Q8D911 Cluster: NAD-dependent malic enzyme; n=187;
cellular organisms|Rep: NAD-dependent malic enzyme -
Vibrio vulnificus
Length = 562
Score = 136 bits (330), Expect = 4e-31
Identities = 79/228 (34%), Positives = 114/228 (50%), Gaps = 1/228 (0%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE + +E+ ++K+IYL + D NE LFYR V +++ EMMPI+YTPTVG AC+ F
Sbjct: 53 QVERAYMQYKAFESDMDKHIYLRNIQDTNETLFYRLVQNHITEMMPIIYTPTVGAACENF 112
Query: 182 GLVYRRPRGLFITIHDKGHVYDVL-RTGRRRTSAPLL*PTENVFWAWAISARAGWASLWA 358
+YRR RGLF++ ++ + D+L ++ E + +
Sbjct: 113 SNIYRRGRGLFVSYANRDRIDDILNNASNHNVKVIVVTDGERILGLGDQGIGGMGIPIGK 172
Query: 359 NSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVV 538
S T S ++ L +P R ++EF++AV
Sbjct: 173 LSLYTACGGISPAYTLPIVLDVGTNNPQRLADPMYMGWRHPRITGADYDAFVEEFIQAVQ 232
Query: 539 RRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
RR+ + L+QFEDF NA LLE+Y N+ C FNDDIQGTAAV V L
Sbjct: 233 RRW-PDALIQFEDFAQKNAMPLLERYKNRICCFNDDIQGTAAVTVGSL 279
Score = 97.9 bits (233), Expect = 2e-19
Identities = 46/73 (63%), Positives = 54/73 (73%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
+V+ IVVTDGERILGLGD G GMGIP+GKL+LYTA GGI P LPI +DVGT L
Sbjct: 143 NVKVIVVTDGERILGLGDQGIGGMGIPIGKLSLYTACGGISPAYTLPIVLDVGTNNPQRL 202
Query: 451 DDPLYIGLRQRRV 489
DP+Y+G R R+
Sbjct: 203 ADPMYMGWRHPRI 215
>UniRef50_Q0AIF8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Nitrosomonas|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Nitrosomonas
eutropha (strain C71)
Length = 536
Score = 134 bits (323), Expect = 2e-30
Identities = 87/227 (38%), Positives = 122/227 (53%), Gaps = 15/227 (6%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
+ KYIYL LL+RN+ LFYR + D++ E+MP+VYTPTVG AC K ++R+P+G +IT
Sbjct: 60 IEKYIYLNDLLERNQQLFYRTLVDHIGEIMPLVYTPTVGEACVKLSHIFRKPQGFYITPE 119
Query: 227 DKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWANSRSTPRSEASSLISV 406
D+G + L+ T ++ T+ G L AN P + IS+
Sbjct: 120 DRGEIISRLKNW-PETDVQIIVVTDG-------ERILGLGDLGANGMGIPIGK----ISL 167
Query: 407 YPSLSTWVRSPSRCWTTRCTSGSGS----------GACWPRL-----RRLLDEFMRAVVR 541
Y + + P RC G+G+ G +PRL L+DEF+ AV
Sbjct: 168 YVACAGIY--PDRCMPVMLDVGTGNQALREDPLYLGYPYPRLVGQDYLSLVDEFVEAVQH 225
Query: 542 RYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
RY + L+QFEDF + NAF LL++Y FNDDIQGTAAV +AG+
Sbjct: 226 RY-PDALIQFEDFSSRNAFTLLDRYAASVRCFNDDIQGTAAVTLAGI 271
Score = 114 bits (274), Expect = 2e-24
Identities = 49/79 (62%), Positives = 63/79 (79%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPETDV+ IVVTDGERILGLGD GA GMGIP+GK++LY A GI P +C+P+ +DVGT
Sbjct: 130 NWPETDVQIIVVTDGERILGLGDLGANGMGIPIGKISLYVACAGIYPDRCMPVMLDVGTG 189
Query: 436 TQSMLDDPLYIGLRQRRVL 492
Q++ +DPLY+G R++
Sbjct: 190 NQALREDPLYLGYPYPRLV 208
>UniRef50_A7T6B8 Cluster: Predicted protein; n=1; Nematostella
vectensis|Rep: Predicted protein - Nematostella
vectensis
Length = 365
Score = 130 bits (313), Expect = 4e-29
Identities = 56/77 (72%), Positives = 64/77 (83%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP T+V+AIV+TDGERILGLGD G CGMGIPVGKLALYT GGI P CLP+ IDVGT
Sbjct: 139 NWPTTEVKAIVMTDGERILGLGDLGCCGMGIPVGKLALYTVCGGIDPEGCLPVMIDVGTN 198
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +LDDP YIG+RQ+R
Sbjct: 199 NEELLDDPFYIGVRQKR 215
Score = 112 bits (269), Expect = 9e-24
Identities = 47/71 (66%), Positives = 60/71 (84%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
N L KYI LM LL+RNE LF+R + D E+MPIVYTPTVGLAC+K+G+++RRPRGLFI+
Sbjct: 67 NDLEKYIQLMALLERNESLFFRVLFDYTEELMPIVYTPTVGLACRKYGMIFRRPRGLFIS 126
Query: 221 IHDKGHVYDVL 253
IHDKGH+ D++
Sbjct: 127 IHDKGHIRDIV 137
>UniRef50_P06801 Cluster: NADP-dependent malic enzyme; n=52;
cellular organisms|Rep: NADP-dependent malic enzyme -
Mus musculus (Mouse)
Length = 572
Score = 129 bits (311), Expect = 7e-29
Identities = 59/77 (76%), Positives = 64/77 (83%)
Frame = +1
Query: 259 WPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTIT 438
WPE V+AIVVTDGERILGLGD G GMGIPVGKLALYTA GG+ P QCLPIT+DVGT
Sbjct: 140 WPEDVVKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGVNPQQCLPITLDVGTEN 199
Query: 439 QSMLDDPLYIGLRQRRV 489
+ +L DPLYIGLR RRV
Sbjct: 200 EELLKDPLYIGLRHRRV 216
Score = 103 bits (247), Expect = 4e-21
Identities = 46/63 (73%), Positives = 49/63 (77%)
Frame = +2
Query: 494 PRLRRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAV 673
P LDEFM A +YG NCL+QFEDF N NAFRLL KY NKYCTFNDDIQGTA+VAV
Sbjct: 219 PEYDAFLDEFMEAASSKYGMNCLIQFEDFANRNAFRLLNKYRNKYCTFNDDIQGTASVAV 278
Query: 674 AGL 682
AGL
Sbjct: 279 AGL 281
Score = 99.1 bits (236), Expect = 9e-20
Identities = 42/77 (54%), Positives = 57/77 (74%)
Frame = +2
Query: 23 SIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP 202
+ + + ++Y+ LM L DRNE LFY + +V + MPIVYTPTVGLACQ++ L +R+P
Sbjct: 61 NFERLNSDFDRYLLLMDLQDRNEKLFYSVLMSDVEKFMPIVYTPTVGLACQQYSLAFRKP 120
Query: 203 RGLFITIHDKGHVYDVL 253
RGLFI+IHDKGH+ VL
Sbjct: 121 RGLFISIHDKGHIASVL 137
>UniRef50_UPI0000D9F768 Cluster: PREDICTED: similar to Y48B6A.12,
partial; n=1; Macaca mulatta|Rep: PREDICTED: similar to
Y48B6A.12, partial - Macaca mulatta
Length = 456
Score = 126 bits (303), Expect = 7e-28
Identities = 83/236 (35%), Positives = 115/236 (48%), Gaps = 9/236 (3%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q C L N + KYIYL L DRNE L+++ + D+VAEMMPIVYTP VG ACQ F
Sbjct: 46 QSRRCYLQFSQNSNDIEKYIYLESLHDRNETLYFKLLVDHVAEMMPIVYTPVVGKACQLF 105
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWAN 361
G ++R RGL+ + +KG+ +++ R A ++ T+ S G L N
Sbjct: 106 GHIFRNARGLYFNLSEKGNFKEMVWNSNVR-DADIIVVTDG-------SRILGLGDLGTN 157
Query: 362 SRSTPRSEASSLI---------SVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLL 514
P + S + +V +L +P +
Sbjct: 158 GMGIPIGKLSLYVACAGINPGRTVPVTLDVGTNNPDLLNDDMYLGERHKRVDGEEYYAAV 217
Query: 515 DEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
DEF+ AV R+ L+QFEDF N +AF LL++Y FNDDIQGT +VA+AGL
Sbjct: 218 DEFVDAVRSRW-PGVLIQFEDFTNDHAFPLLKRYQENILCFNDDIQGTGSVALAGL 272
Score = 93.9 bits (223), Expect = 3e-18
Identities = 42/73 (57%), Positives = 53/73 (72%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D IVVTDG RILGLGD G GMGIP+GKL+LY A GI P + +P+T+DVGT +L
Sbjct: 136 DADIIVVTDGSRILGLGDLGTNGMGIPIGKLSLYVACAGINPGRTVPVTLDVGTNNPDLL 195
Query: 451 DDPLYIGLRQRRV 489
+D +Y+G R +RV
Sbjct: 196 NDDMYLGERHKRV 208
>UniRef50_P48163 Cluster: NADP-dependent malic enzyme; n=63;
Eukaryota|Rep: NADP-dependent malic enzyme - Homo
sapiens (Human)
Length = 572
Score = 126 bits (303), Expect = 7e-28
Identities = 56/77 (72%), Positives = 64/77 (83%)
Frame = +1
Query: 259 WPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTIT 438
WPE ++AIVVTDGERILGLGD G GMGIPVGKLALYTA GG+ P +CLP+ +DVGT
Sbjct: 140 WPEDVIKAIVVTDGERILGLGDLGCNGMGIPVGKLALYTACGGMNPQECLPVILDVGTEN 199
Query: 439 QSMLDDPLYIGLRQRRV 489
+ +L DPLYIGLRQRRV
Sbjct: 200 EELLKDPLYIGLRQRRV 216
Score = 103 bits (246), Expect = 5e-21
Identities = 43/68 (63%), Positives = 56/68 (82%)
Frame = +2
Query: 50 NKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHD 229
++Y+ LM L DRNE LFYR + ++ + MPIVYTPTVGLACQ++ LV+R+PRGLFITIHD
Sbjct: 70 DRYLLLMDLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYSLVFRKPRGLFITIHD 129
Query: 230 KGHVYDVL 253
+GH+ VL
Sbjct: 130 RGHIASVL 137
Score = 102 bits (245), Expect = 7e-21
Identities = 45/57 (78%), Positives = 49/57 (85%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
LDEFM AV +YG NCL+QFEDF N NAFRLL KY N+YCTFNDDIQGTA+VAVAGL
Sbjct: 225 LDEFMEAVSSKYGMNCLIQFEDFANVNAFRLLNKYRNQYCTFNDDIQGTASVAVAGL 281
>UniRef50_P16243 Cluster: NADP-dependent malic enzyme, chloroplast
precursor; n=79; Magnoliophyta|Rep: NADP-dependent malic
enzyme, chloroplast precursor - Zea mays (Maize)
Length = 636
Score = 120 bits (289), Expect = 3e-26
Identities = 54/77 (70%), Positives = 62/77 (80%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP +++ I VTDGERILGLGD G GMGIPVGKLALYTALGG+ P CLPITIDVGT
Sbjct: 221 NWPHRNIQVICVTDGERILGLGDLGCQGMGIPVGKLALYTALGGVDPSVCLPITIDVGTN 280
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+D YIGLRQ+R
Sbjct: 281 NEFLLNDEFYIGLRQKR 297
Score = 98.3 bits (234), Expect = 2e-19
Identities = 41/90 (45%), Positives = 63/90 (70%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q++ ++ Y+ PL +YI +M L + +E LFY+ + DNV E++P VYTPTVG ACQK+
Sbjct: 136 QIKKFMNTLRQYQTPLQRYIAMMNLQETDERLFYKLLIDNVVELLPFVYTPTVGEACQKY 195
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRR 271
G ++ RP+GL++++ DKG V +VLR R
Sbjct: 196 GSIFGRPQGLYVSLKDKGKVLEVLRNWPHR 225
Score = 79.4 bits (187), Expect = 7e-14
Identities = 36/58 (62%), Positives = 44/58 (75%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L++EFM AV + YG+ L+QFEDF N NAF LLEKY + FNDDIQGTA+V +AGL
Sbjct: 306 LIEEFMSAVKQFYGEKVLIQFEDFANHNAFDLLEKYSKSHLVFNDDIQGTASVVLAGL 363
>UniRef50_A1ZAF7 Cluster: Malic enzyme; n=5; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 603
Score = 120 bits (288), Expect = 4e-26
Identities = 70/226 (30%), Positives = 104/226 (46%), Gaps = 1/226 (0%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q+ + + + ++ YL + R+ L+YRF+ +N+ + +PIVYTPTVG +
Sbjct: 77 QMNAALANFEARPTDIARFTYLSAVHHRHRRLYYRFIKENIEKSLPIVYTPTVGDVVATY 136
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTG-RRRTSAPLL*PTENVFWAWAISARAGWASLWA 358
GL +++ LFI+IHDKGH+ D++ A + V + A A SL
Sbjct: 137 GLNFQQAISLFISIHDKGHIRDLMHNWVDEGVKAICVTDGGRVLGLGDMGANAMGISLGK 196
Query: 359 NSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVV 538
T + L + + P L+DEFM + V
Sbjct: 197 MILYTALGSIPPSTLMPVCLDVGTDNQALLQDPLYVGARIPRVKGPEYDELVDEFMESAV 256
Query: 539 RRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
+ +G N + FEDF NA + LEKY KYC FNDDIQGT A +A
Sbjct: 257 KCFGNNTFIHFEDFATPNALKFLEKYQYKYCCFNDDIQGTGATGLA 302
Score = 99.1 bits (236), Expect = 9e-20
Identities = 45/78 (57%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NW + V+AI VTDG R+LGLGD GA MGI +GK+ LYTALG I P +P+ +DVGT
Sbjct: 162 NWVDEGVKAICVTDGGRVLGLGDMGANAMGISLGKMILYTALGSIPPSTLMPVCLDVGTD 221
Query: 436 TQSMLDDPLYIGLRQRRV 489
Q++L DPLY+G R RV
Sbjct: 222 NQALLQDPLYVGARIPRV 239
>UniRef50_A7PC00 Cluster: Chromosome chr2 scaffold_11, whole genome
shotgun sequence; n=3; Vitis vinifera|Rep: Chromosome
chr2 scaffold_11, whole genome shotgun sequence - Vitis
vinifera (Grape)
Length = 573
Score = 118 bits (284), Expect = 1e-25
Identities = 52/77 (67%), Positives = 61/77 (79%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPE ++ IVVT+GERILGLGD G GMGIPVGKL+LYT LGG+ P CLP+TIDVGT
Sbjct: 321 NWPERSIQVIVVTNGERILGLGDLGCQGMGIPVGKLSLYTTLGGLHPSVCLPVTIDVGTN 380
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L D YIGL+QRR
Sbjct: 381 NEQLLKDEFYIGLKQRR 397
Score = 89.0 bits (211), Expect = 9e-17
Identities = 37/80 (46%), Positives = 56/80 (70%)
Frame = +2
Query: 35 YENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLF 214
Y+ PL +Y+ +M +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++RRP+ L+
Sbjct: 247 YKVPLQRYMAMMDFQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRRPQSLY 306
Query: 215 ITIHDKGHVYDVLRTGRRRT 274
I G + +VL+ R+
Sbjct: 307 IIDFFMGKILEVLKNWPERS 326
>UniRef50_P23368 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=53; Eumetazoa|Rep: NAD-dependent malic
enzyme, mitochondrial precursor - Homo sapiens (Human)
Length = 584
Score = 118 bits (283), Expect = 2e-25
Identities = 52/77 (67%), Positives = 61/77 (79%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPE V+A+VVTDGERILGLGD G GMGIPVGKL LYTA GI+P +CLP+ IDVGT
Sbjct: 149 NWPENHVKAVVVTDGERILGLGDLGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTD 208
Query: 436 TQSMLDDPLYIGLRQRR 486
++L DP Y+GL Q+R
Sbjct: 209 NIALLKDPFYMGLYQKR 225
Score = 105 bits (253), Expect = 8e-22
Identities = 42/71 (59%), Positives = 59/71 (83%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
+PL KYIY+MG+ +RNE LFYR + D++ +MPIVYTPTVGLAC ++G ++RRP+GLFI+
Sbjct: 77 SPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACSQYGHIFRRPKGLFIS 136
Query: 221 IHDKGHVYDVL 253
I D+GHV ++
Sbjct: 137 ISDRGHVRSIV 147
Score = 101 bits (241), Expect = 2e-20
Identities = 44/58 (75%), Positives = 50/58 (86%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+DEFM+A+ RYG+N L+QFEDFGN NAFR L KY KYCTFNDDIQGTAAVA+AGL
Sbjct: 234 LIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYREKYCTFNDDIQGTAAVALAGL 291
>UniRef50_Q00XN9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus tauri
Length = 639
Score = 117 bits (282), Expect = 2e-25
Identities = 73/221 (33%), Positives = 111/221 (50%), Gaps = 6/221 (2%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
E PL+K+ +L L DRNE LFYR V D++ E+ PI+YTPTVG AC F + RR RG++
Sbjct: 132 ERPLDKWQHLQDLKDRNETLFYRLVHDHIEELAPIIYTPTVGDACLNFSKLLRRARGMYF 191
Query: 218 TIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARA-----GWASLW-ANSRSTPR 379
++ D+G + ++ +R S ++ + + G L+ A P+
Sbjct: 192 SVDDRGDINSMMFNWKRSVSVIVVTDGSRILGLGDLGTNGMGISQGKVDLYVAGGGFDPQ 251
Query: 380 SEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVVRRYGQNC 559
++ V + + P R ++EF+R V R+ N
Sbjct: 252 HVLPVVLDVGTNNEDLLNDPYYLGVPRKRLEG------EEYYAFIEEFIRGVRNRW-PNA 304
Query: 560 LVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+QFEDF +A ++L++Y N FNDDIQGTAAV +AGL
Sbjct: 305 LIQFEDFQTKHANKILQRYRNDVLCFNDDIQGTAAVVLAGL 345
Score = 88.6 bits (210), Expect = 1e-16
Identities = 41/78 (52%), Positives = 53/78 (67%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NW + V IVVTDG RILGLGD G GMGI GK+ LY A GG P LP+ +DVGT
Sbjct: 205 NWKRS-VSVIVVTDGSRILGLGDLGTNGMGISQGKVDLYVAGGGFDPQHVLPVVLDVGTN 263
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L+DP Y+G+ ++R+
Sbjct: 264 NEDLLNDPYYLGVPRKRL 281
>UniRef50_Q4S0L0 Cluster: Malic enzyme; n=2; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 694
Score = 115 bits (277), Expect = 9e-25
Identities = 50/79 (63%), Positives = 63/79 (79%)
Frame = +2
Query: 23 SIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP 202
S +T NPL+KYI LM L DRNE LFYR + ++ + MPIVYTPTVGLACQ++GL +RRP
Sbjct: 108 SYETRSNPLDKYILLMTLQDRNEKLFYRVLTSDIEKFMPIVYTPTVGLACQQYGLAFRRP 167
Query: 203 RGLFITIHDKGHVYDVLRT 259
RGLFITIHD+GH+ +L +
Sbjct: 168 RGLFITIHDRGHIATMLNS 186
Score = 88.2 bits (209), Expect = 2e-16
Identities = 35/49 (71%), Positives = 43/49 (87%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQG 655
L++EFM+AV +YG NCL+QFEDF N+NAFR+L KY N+YCTFNDDIQG
Sbjct: 281 LIEEFMQAVTDKYGMNCLIQFEDFANSNAFRILNKYRNRYCTFNDDIQG 329
Score = 87.4 bits (207), Expect = 3e-16
Identities = 46/88 (52%), Positives = 57/88 (64%), Gaps = 10/88 (11%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALY-----TALGGIKPHQC----- 405
+WPE D++A+VVTDGERILGLGD G+ GMGIPVGKLAL A + P
Sbjct: 186 SWPEEDIKAVVVTDGERILGLGDLGSYGMGIPVGKLALLHRLRRRAAAAVPPGAAGRGHR 245
Query: 406 LPITIDVGTITQSMLDDPLYIGLRQRRV 489
P ++ G Q +LDDPLYIGL+ +RV
Sbjct: 246 QPGSVPGGVCVQVLLDDPLYIGLKHKRV 273
>UniRef50_A7IMB8 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=2;
Alphaproteobacteria|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Xanthobacter
sp. (strain Py2)
Length = 550
Score = 113 bits (273), Expect = 3e-24
Identities = 78/233 (33%), Positives = 121/233 (51%), Gaps = 6/233 (2%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE +D ++ L++Y YLM L RNE +FY+ V + +PI+Y PTV AC+ F
Sbjct: 58 QVERVLDHLDHVKDELDQYSYLMDLEARNETVFYKAVMSDPKRFIPILYDPTVADACEAF 117
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PT-ENVFWAWAISARA-----GW 343
G +YRRPRG++IT H KG + +VLR ++ + T + I A G
Sbjct: 118 GNLYRRPRGMYITRHMKGRMAEVLRNWPQKDIRFVCVSTGGRILGLGDIGANGMGIPIGK 177
Query: 344 ASLWANSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEF 523
L+ + P ++ + + +R+ TR S + L L +EF
Sbjct: 178 LQLYTACAAVPPDVLLPVLFDIGTSNEHLRADPFYLGTREAPVSEA-----ELDELTEEF 232
Query: 524 MRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++AV + C V FED+ +A R+L++Y +K +NDDIQGTA+VA+AG+
Sbjct: 233 VQAVNEVF-PGCCVHFEDWKGTDAIRMLDRYKDKILCYNDDIQGTASVALAGI 284
Score = 93.9 bits (223), Expect = 3e-18
Identities = 40/78 (51%), Positives = 52/78 (66%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP+ D+R + V+ G RILGLGD GA GMGIP+GKL LYTA + P LP+ D+GT
Sbjct: 143 NWPQKDIRFVCVSTGGRILGLGDIGANGMGIPIGKLQLYTACAAVPPDVLLPVLFDIGTS 202
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ + DP Y+G R+ V
Sbjct: 203 NEHLRADPFYLGTREAPV 220
>UniRef50_A6SA55 Cluster: Malic enzyme; n=2; Sclerotiniaceae|Rep:
Malic enzyme - Botryotinia fuckeliana B05.10
Length = 685
Score = 110 bits (265), Expect = 3e-23
Identities = 70/235 (29%), Positives = 115/235 (48%), Gaps = 8/235 (3%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q C ++ + P+ KY+YL L + N HLFYR V +++ ++ P++YTPTVG AC ++
Sbjct: 129 QTTRCLQQLNAKKTPIEKYMYLSNLRNNNVHLFYRLVQEHLTDITPLIYTPTVGEACLRW 188
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWAN 361
+Y++P GL+++ HD+G++ +VL R++ + T+ S G L N
Sbjct: 189 SEIYQQPEGLYLSYHDRGNLEEVLGNW-RQSDVEMTVVTDG-------SRILGLGDLGVN 240
Query: 362 SRSTPRSEAS---SLISVYPSLSTWV-----RSPSRCWTTRCTSGSGSGACWPRLRRLLD 517
P + S ++PS + + + + G+ +
Sbjct: 241 GMGIPVGKLSLYTGCAGIHPSKTLPITLDLGTNNEKFLKDPLYMGNRMKRVSTKEEAEFM 300
Query: 518 EFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
E + + + +VQ+EDF N F L KY NKY FNDDIQGT AV +AG+
Sbjct: 301 EELMVALNKMWPGIIVQYEDF--KNPFPSLAKYQNKYPCFNDDIQGTGAVILAGI 353
Score = 105 bits (253), Expect = 8e-22
Identities = 48/78 (61%), Positives = 57/78 (73%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NW ++DV VVTDG RILGLGD G GMGIPVGKL+LYT GI P + LPIT+D+GT
Sbjct: 214 NWRQSDVEMTVVTDGSRILGLGDLGVNGMGIPVGKLSLYTGCAGIHPSKTLPITLDLGTN 273
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ L DPLY+G R +RV
Sbjct: 274 NEKFLKDPLYMGNRMKRV 291
>UniRef50_A7CWP9 Cluster: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=1;
Opitutaceae bacterium TAV2|Rep: Malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)) - Opitutaceae
bacterium TAV2
Length = 561
Score = 109 bits (263), Expect = 5e-23
Identities = 49/78 (62%), Positives = 59/78 (75%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
+WP TDVR IVVTDGERILGLGD G GMGIPVGKLALY+A G+ P CLPI +D G
Sbjct: 148 HWPITDVRMIVVTDGERILGLGDLGVLGMGIPVGKLALYSACAGLHPSYCLPIALDAGID 207
Query: 436 TQSMLDDPLYIGLRQRRV 489
+++ +DPLY+G +RV
Sbjct: 208 NETLRNDPLYLGHPMKRV 225
Score = 91.5 bits (217), Expect = 2e-17
Identities = 39/70 (55%), Positives = 53/70 (75%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
+ KYIYL L RNE LFYR + ++ EM+P+VYTPTVG AC ++G +RRPRGLFI+I
Sbjct: 78 IEKYIYLTTLQSRNETLFYRLLTNHAEEMIPLVYTPTVGQACLEYGANFRRPRGLFISIK 137
Query: 227 DKGHVYDVLR 256
D+G + ++LR
Sbjct: 138 DRGRIAEILR 147
Score = 68.9 bits (161), Expect = 1e-10
Identities = 32/57 (56%), Positives = 45/57 (78%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+EF+ AV + + ++Q+EDFGN NAFRLL + ++ C+FNDDIQGTAAVA+AG+
Sbjct: 234 LNEFVEAVKTVFPKT-MIQWEDFGNTNAFRLLHENRSRVCSFNDDIQGTAAVALAGV 289
>UniRef50_Q016K2 Cluster: NADP dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP dependent malic enzyme -
Ostreococcus tauri
Length = 641
Score = 107 bits (257), Expect = 2e-22
Identities = 71/219 (32%), Positives = 110/219 (50%), Gaps = 7/219 (3%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
+ K+ +L L +RNE LFYR V D++ E++P++ PTV C++ GL+YR+PRGL++++
Sbjct: 137 VEKHAWLPALYERNERLFYRVVKDHLEELLPVLAEPTVWQVCREAGLMYRQPRGLYVSMQ 196
Query: 227 DKGHVYDVLRTGR-RRTSAPLL*PTENV-----FWAWAISARAGWASLW-ANSRSTPRSE 385
DKG VY +L+ R A +L + V + A ASL+ A P
Sbjct: 197 DKGSVYRLLKNWPVRNVKAVVLTDGQRVTGIGDLGVQGMPAAVSKASLFTALGGLDPADV 256
Query: 386 ASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVVRRYGQNCLV 565
I V T + + G + LLDE + + RR+G L+
Sbjct: 257 LPICIDVGTDNQTLLEDKFYIGLRQKRVGGEA------YEELLDEVVSGLKRRFGPRVLL 310
Query: 566 QFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
FE+F NANA +LL +Y + DD+QG AA+ +A +
Sbjct: 311 CFEEFSNANAKKLLSRYNRDSVAYVDDLQGVAAITLAAV 349
Score = 101 bits (242), Expect = 2e-20
Identities = 46/86 (53%), Positives = 61/86 (70%)
Frame = +1
Query: 232 GTCIRCS*NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLP 411
G+ R NWP +V+A+V+TDG+R+ G+GD G GM V K +L+TALGG+ P LP
Sbjct: 199 GSVYRLLKNWPVRNVKAVVLTDGQRVTGIGDLGVQGMPAAVSKASLFTALGGLDPADVLP 258
Query: 412 ITIDVGTITQSMLDDPLYIGLRQRRV 489
I IDVGT Q++L+D YIGLRQ+RV
Sbjct: 259 ICIDVGTDNQTLLEDKFYIGLRQKRV 284
>UniRef50_Q4QAQ6 Cluster: Malic enzyme, putative; n=20;
Trypanosomatidae|Rep: Malic enzyme, putative -
Leishmania major
Length = 573
Score = 106 bits (255), Expect = 4e-22
Identities = 73/234 (31%), Positives = 117/234 (50%), Gaps = 8/234 (3%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE ++ + P+N+Y L + + N L+Y + + + +PIVYTPTVG ACQ++
Sbjct: 58 QVERYWDQLNRFNEPINRYQLLRNVQNTNVTLYYAILTRYLKQTLPIVYTPTVGEACQRY 117
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENV-------FWAWAISARAG 340
G +Y++ GL++ + KG V +++ R+T+ ++ T+ + I G
Sbjct: 118 GDLYQKDHGLYLDVASKGKVRRLIQ-NLRKTNIDVIVITDGSRILGLGDLGSNGIGISIG 176
Query: 341 WASLWANSRSTPRSEASSLISVYPSLSTWVR-SPSRCWTTRCTSGSGSGACWPRLRRLLD 517
SL+ + S ++ + + +R +P + G LLD
Sbjct: 177 KCSLYVAAGGVKPSRVLPVVMDVGTNNLELRNNPLYLGLRKPRCGDAD------FYALLD 230
Query: 518 EFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
EFM AV + + +VQFEDF N + F +LE+Y KY FNDDIQGT AV AG
Sbjct: 231 EFMEAVKDTW-PSAVVQFEDFSNNHCFDMLERYQKKYRCFNDDIQGTGAVIAAG 283
Score = 92.3 bits (219), Expect = 1e-17
Identities = 40/77 (51%), Positives = 57/77 (74%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
N +T++ IV+TDG RILGLGD G+ G+GI +GK +LY A GG+KP + LP+ +DVGT
Sbjct: 143 NLRKTNIDVIVITDGSRILGLGDLGSNGIGISIGKCSLYVAAGGVKPSRVLPVVMDVGTN 202
Query: 436 TQSMLDDPLYIGLRQRR 486
+ ++PLY+GLR+ R
Sbjct: 203 NLELRNNPLYLGLRKPR 219
>UniRef50_Q86NT5 Cluster: Malic enzyme; n=2; Drosophila
melanogaster|Rep: Malic enzyme - Drosophila melanogaster
(Fruit fly)
Length = 610
Score = 105 bits (253), Expect = 8e-22
Identities = 51/79 (64%), Positives = 59/79 (74%), Gaps = 1/79 (1%)
Frame = +1
Query: 256 NWP-ETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGT 432
NWP V+AI VTDG+RILGLGD GA GMGI VGK+ LYTAL GI P LPI +DVGT
Sbjct: 170 NWPMRRSVKAICVTDGQRILGLGDLGANGMGIAVGKMELYTALAGIPPSMLLPICLDVGT 229
Query: 433 ITQSMLDDPLYIGLRQRRV 489
+S+ +DPLYIGLR R+
Sbjct: 230 NNKSLHEDPLYIGLRDERL 248
Score = 81.4 bits (192), Expect = 2e-14
Identities = 36/90 (40%), Positives = 57/90 (63%)
Frame = +2
Query: 32 TYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGL 211
++E+ + +Y YL L E L+++FV+ NV ++PI+YTPTVGLAC +G++YR G+
Sbjct: 95 SFESNVGRYRYLRALRQGYERLYFQFVSKNVHAVLPIIYTPTVGLACTVYGMLYRGMTGI 154
Query: 212 FITIHDKGHVYDVLRTGRRRTSAPLL*PTE 301
IT HD+GH+ +L R S + T+
Sbjct: 155 HITKHDRGHMKQILSNWPMRRSVKAICVTD 184
Score = 75.4 bits (177), Expect = 1e-12
Identities = 33/57 (57%), Positives = 39/57 (68%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+DEFM AVV +G L+ FEDF NAF L +Y + YC FNDDIQGTAAV + GL
Sbjct: 257 VDEFMEAVVSTFGDQTLIHFEDFATPNAFMFLNRYQHCYCHFNDDIQGTAAVGLGGL 313
>UniRef50_Q875H8 Cluster: Malic enzyme; n=1; Mucor
circinelloides|Rep: Malic enzyme - Mucor circinelloides
Length = 617
Score = 105 bits (253), Expect = 8e-22
Identities = 46/78 (58%), Positives = 57/78 (73%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP V IVVTDG R+LGLGD GA GM IP+GKL+LY A GGI+P LP+ +DVGT
Sbjct: 174 NWPHDKVDVIVVTDGSRVLGLGDLGANGMEIPIGKLSLYVAAGGIRPRAVLPVVLDVGTN 233
Query: 436 TQSMLDDPLYIGLRQRRV 489
Q +L+DPLY+G+ R+
Sbjct: 234 NQDLLNDPLYLGMSHPRL 251
Score = 77.4 bits (182), Expect = 3e-13
Identities = 32/75 (42%), Positives = 51/75 (68%)
Frame = +2
Query: 17 KLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR 196
K ++D E PL K+++L L DRNE L+Y+ + +++ E+ I+YTPTVGLA Q +YR
Sbjct: 94 KRNLDACETPLAKFVFLAALHDRNETLYYKIIMEHLEELAGIIYTPTVGLASQMSHSIYR 153
Query: 197 RPRGLFITIHDKGHV 241
R RG++ + D+G +
Sbjct: 154 RSRGMYFSSQDRGQM 168
Score = 57.2 bits (132), Expect = 3e-07
Identities = 25/57 (43%), Positives = 40/57 (70%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+DE++ A+ R+ + L+QFEDF +A+ LL KY N+ FNDDIQ T+++ +AG+
Sbjct: 260 IDEWVTAITSRW-PDTLIQFEDFKYPHAYNLLAKYRNRITCFNDDIQSTSSITLAGI 315
>UniRef50_Q9HE50 Cluster: Malic enzyme; n=6; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 1023
Score = 105 bits (252), Expect = 1e-21
Identities = 47/78 (60%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP VVTDG RILGLGD G G+GIP+GKLALYTA GI P + LPI +D GT
Sbjct: 596 NWPYPQPEICVVTDGSRILGLGDLGVNGVGIPIGKLALYTAAAGIHPDKTLPIVLDCGTA 655
Query: 436 TQSMLDDPLYIGLRQRRV 489
++ L DPLY+GLRQ+R+
Sbjct: 656 NETNLKDPLYLGLRQKRI 673
Score = 76.2 bits (179), Expect = 7e-13
Identities = 33/85 (38%), Positives = 52/85 (61%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE I T + +++Y+YL + +N LFYR + D+ EMMP+VYTPT+G C ++
Sbjct: 511 QVERALKMIRTRQTDIDRYLYLSTIKSQNVDLFYRLLMDHAKEMMPLVYTPTIGDVCLQY 570
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLR 256
+Y RP L+I+I + + +LR
Sbjct: 571 STLYTRPEALYISIKQRKSIRTILR 595
Score = 60.1 bits (139), Expect = 5e-08
Identities = 28/59 (47%), Positives = 39/59 (66%)
Frame = +2
Query: 503 RRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
+ +DEFM A + + +VQFEDF + AF L++Y ++Y FNDDIQGT AV +AG
Sbjct: 679 QEFMDEFMEAAAEVF-PDMVVQFEDFESEKAFNYLDRYRDQYKCFNDDIQGTGAVVLAG 736
>UniRef50_Q4X1Z2 Cluster: NADP-dependent malic enzyme MaeA; n=11;
Pezizomycotina|Rep: NADP-dependent malic enzyme MaeA -
Aspergillus fumigatus (Sartorya fumigata)
Length = 661
Score = 104 bits (249), Expect = 2e-21
Identities = 43/78 (55%), Positives = 59/78 (75%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP+ +V +TDG RILGLGD G GMGIP+GKLALYTA GI+P LP+T+D+GT
Sbjct: 206 NWPQPNVEITCITDGSRILGLGDLGINGMGIPIGKLALYTACAGIRPEATLPLTLDLGTS 265
Query: 436 TQSMLDDPLYIGLRQRRV 489
+++ +DPLY+G R+ ++
Sbjct: 266 NKALREDPLYMGTRRDKI 283
Score = 78.2 bits (184), Expect = 2e-13
Identities = 30/80 (37%), Positives = 53/80 (66%)
Frame = +2
Query: 14 CKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVY 193
C + + ++K++YL L N HLFYR V D++ E+ P++YTP VG ACQK+ +Y
Sbjct: 125 CLAQLALKQTAIDKFLYLSTLRKNNVHLFYRLVTDHLKELTPLIYTPVVGEACQKWSEIY 184
Query: 194 RRPRGLFITIHDKGHVYDVL 253
++P G++++ D+G++ V+
Sbjct: 185 QQPEGMYLSWEDRGNLAAVI 204
Score = 55.6 bits (128), Expect = 1e-06
Identities = 28/60 (46%), Positives = 37/60 (61%)
Frame = +2
Query: 503 RRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
R +DE M A+ R+ ++QFEDF N F LE+Y + Y FNDDIQGT AV + G+
Sbjct: 289 REFMDELMAALTERW-PGIVIQFEDF--KNPFPALERYRDAYTCFNDDIQGTGAVILGGV 345
>UniRef50_P78715 Cluster: Malic enzyme, hydrogenosomal precursor;
n=1; Neocallimastix frontalis|Rep: Malic enzyme,
hydrogenosomal precursor - Neocallimastix frontalis
(Rumen fungus)
Length = 592
Score = 104 bits (249), Expect = 2e-21
Identities = 47/78 (60%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP DV IVVTDG RILGLGD GA GM IP+GKL LY GGI P LPI +DVGT
Sbjct: 171 NWPYDDVDVIVVTDGSRILGLGDLGAGGMQIPIGKLTLYVCGGGINPRNVLPIVLDVGTN 230
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L+DPLY+G++ R+
Sbjct: 231 NKELLNDPLYLGMQHPRL 248
Score = 85.0 bits (201), Expect = 2e-15
Identities = 34/80 (42%), Positives = 54/80 (67%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q + CK ++D +PL K+IYL L +RNE L+Y+ + +N E+ PI+YTP VG ACQKF
Sbjct: 86 QYKRCKTNLDKISDPLEKFIYLNHLQNRNETLYYKMILENFVELAPIIYTPVVGEACQKF 145
Query: 182 GLVYRRPRGLFITIHDKGHV 241
++ + RG++ + D+G +
Sbjct: 146 HKIFTQTRGMYFSTADRGQM 165
Score = 54.0 bits (124), Expect = 3e-06
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+DE++ A+ R+ + ++QFEDF NA LL KY ++ C FNDDIQ T A+ +A +
Sbjct: 257 VDEWVSAITDRFPK-AVIQFEDFMMPNALDLLLKYKDQICMFNDDIQSTGAITLASV 312
>UniRef50_Q8I8I4 Cluster: Malic enzyme; n=4; Eukaryota|Rep: Malic
enzyme - Mastigamoeba balamuthi (Phreatamoeba balamuthi)
Length = 568
Score = 103 bits (248), Expect = 3e-21
Identities = 47/78 (60%), Positives = 59/78 (75%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP +V IVVTDG RILGLGD G+ GMGIP+GKL LY A G +P + LP+ IDVGT
Sbjct: 159 NWPH-EVEIIVVTDGGRILGLGDLGSNGMGIPIGKLHLYIACAGFRPDRTLPVMIDVGTN 217
Query: 436 TQSMLDDPLYIGLRQRRV 489
Q +LDDP+Y+G+R+ R+
Sbjct: 218 RQELLDDPMYLGVRKARL 235
Score = 82.2 bits (194), Expect = 1e-14
Identities = 37/70 (52%), Positives = 47/70 (67%)
Frame = +2
Query: 44 PLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 223
PL KY+YL L RN+ LF+ V +V E +P+VYTPTVG C KF +R P GL+IT
Sbjct: 88 PLLKYLYLSQLSQRNQTLFFYLVQHHVEECVPLVYTPTVGEGCTKFSAEFRNPTGLYITP 147
Query: 224 HDKGHVYDVL 253
DKGHV ++L
Sbjct: 148 EDKGHVAEIL 157
Score = 63.7 bits (148), Expect = 4e-09
Identities = 31/58 (53%), Positives = 41/58 (70%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
LL+EFM AV ++ + CLVQFEDF N F+LL+ + + FNDDIQGT AV +AG+
Sbjct: 243 LLEEFMTAVRAKWPR-CLVQFEDFPNPRCFQLLDTWFGRQFCFNDDIQGTGAVILAGV 299
>UniRef50_Q5BX10 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 216
Score = 103 bits (248), Expect = 3e-21
Identities = 44/84 (52%), Positives = 62/84 (73%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV ++ + L +YIYL L DRNE LFY+ V ++V MP++YTPTVGLACQ++
Sbjct: 93 QVSKMLANLKNMNDNLQRYIYLTSLQDRNEALFYKLVIEHVEYCMPLIYTPTVGLACQRY 152
Query: 182 GLVYRRPRGLFITIHDKGHVYDVL 253
G+V+RRPRGL+ITIHD+ H+ ++L
Sbjct: 153 GVVFRRPRGLYITIHDRHHIPEIL 176
Score = 62.1 bits (144), Expect = 1e-08
Identities = 27/34 (79%), Positives = 29/34 (85%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVG 357
NWPE V+AIV TDGERILGLGD GA GMGIP+G
Sbjct: 178 NWPEPIVKAIVFTDGERILGLGDLGAYGMGIPIG 211
>UniRef50_Q01AM5 Cluster: NADP+-dependent malic enzyme; n=2;
Ostreococcus|Rep: NADP+-dependent malic enzyme -
Ostreococcus tauri
Length = 580
Score = 103 bits (247), Expect = 4e-21
Identities = 48/77 (62%), Positives = 57/77 (74%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP TDV+ V+TDGERILGLGD GA GMGI GK +Y A G+ P LPI +D GT
Sbjct: 170 NWPATDVKIAVITDGERILGLGDQGANGMGISAGKSMVYAAC-GVPPSALLPIQVDTGTN 228
Query: 436 TQSMLDDPLYIGLRQRR 486
Q++LDDPLYIGL+Q+R
Sbjct: 229 NQTLLDDPLYIGLKQKR 245
Score = 70.5 bits (165), Expect = 3e-11
Identities = 31/73 (42%), Positives = 46/73 (63%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
E P KY L+ L +E FYR + ++PI+YTPTVG AC KFG + +RP GL++
Sbjct: 97 ETPFEKYKQLVALQMTDESTFYRMLRSQTETLLPILYTPTVGEACVKFGTLVQRPMGLWV 156
Query: 218 TIHDKGHVYDVLR 256
+ +D G+V ++R
Sbjct: 157 SSNDAGNVKQLIR 169
Score = 65.7 bits (153), Expect = 1e-09
Identities = 32/60 (53%), Positives = 42/60 (70%), Gaps = 2/60 (3%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMN--KYCTFNDDIQGTAAVAVAGL 682
LLDE + AV RYGQ ++ +EDF NAFR+L ++ + T+NDDIQGTAAV V+GL
Sbjct: 254 LLDEIVVAVRSRYGQKTIIHWEDFAPKNAFRVLRRFQQAPEVVTYNDDIQGTAAVTVSGL 313
>UniRef50_A3BK03 Cluster: Malic enzyme; n=2; Oryza sativa|Rep: Malic
enzyme - Oryza sativa subsp. japonica (Rice)
Length = 635
Score = 100 bits (240), Expect = 3e-20
Identities = 45/78 (57%), Positives = 55/78 (70%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP V IVVTDG RILGLGD G G+GI +GKL LY A GI P + LP+ IDVGT
Sbjct: 217 NWPADQVDMIVVTDGSRILGLGDLGVHGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTN 276
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L DPLY+GL++ R+
Sbjct: 277 NEKLLKDPLYLGLQEHRL 294
Score = 73.7 bits (173), Expect = 4e-12
Identities = 35/77 (45%), Positives = 51/77 (66%), Gaps = 2/77 (2%)
Frame = +2
Query: 29 DTYENPLNKYIYLMGLLDRNEHLFYR--FVADNVAEMMPIVYTPTVGLACQKFGLVYRRP 202
DTY PL K+ L L DRNE ++Y+ + DN+ E PIVYTPTVGL CQ + ++RRP
Sbjct: 141 DTY--PLAKWRILNRLHDRNETMYYKAEVLIDNIEEHAPIVYTPTVGLVCQNYSGLFRRP 198
Query: 203 RGLFITIHDKGHVYDVL 253
RG++ + D+G + ++
Sbjct: 199 RGMYFSAEDRGEMMSMV 215
Score = 55.2 bits (127), Expect = 1e-06
Identities = 26/48 (54%), Positives = 34/48 (70%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQ 652
++DEFM AV R+ N +VQFEDF + AFRLL++Y Y FNDD+Q
Sbjct: 302 IIDEFMEAVFARW-PNVIVQFEDFQSKWAFRLLQRYRKTYRMFNDDVQ 348
>UniRef50_A3QW96 Cluster: Malic enzyme; n=10; Tigriopus
californicus|Rep: Malic enzyme - Tigriopus californicus
(Marine copepod)
Length = 322
Score = 100 bits (239), Expect = 4e-20
Identities = 42/78 (53%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NW +V A+V+TDG RILGLGD G G+GI +GKL LY A GG P + LP+ +D+GT
Sbjct: 188 NWESDEVDAVVITDGSRILGLGDLGLGGLGISIGKLDLYVAAGGFHPRRVLPVVLDIGTN 247
Query: 436 TQSMLDDPLYIGLRQRRV 489
Q +L+DP Y+GL+Q R+
Sbjct: 248 NQKLLNDPNYLGLKQPRI 265
Score = 76.6 bits (180), Expect = 5e-13
Identities = 32/65 (49%), Positives = 44/65 (67%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
+ K+ L + DRNE LFYR + DN +M PI+YTPTVG AC F +YRRPRG++ +
Sbjct: 118 IRKWKVLQSVQDRNETLFYRILMDNFQDMAPIIYTPTVGWACSHFSQLYRRPRGMYFSHG 177
Query: 227 DKGHV 241
D+G +
Sbjct: 178 DRGEM 182
Score = 51.2 bits (117), Expect = 2e-05
Identities = 24/51 (47%), Positives = 35/51 (68%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTA 661
++DE M A R+ + L+Q EDF + +A LL++Y ++Y FNDDIQGTA
Sbjct: 273 IMDELMAATKLRWPR-ALIQHEDFXSKHAVNLLKRYRSEYLMFNDDIQGTA 322
>UniRef50_A2EKE3 Cluster: Malic enzyme; n=14; Trichomonadidae|Rep:
Malic enzyme - Trichomonas vaginalis G3
Length = 567
Score = 100 bits (239), Expect = 4e-20
Identities = 46/78 (58%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
N+P D+R IVVTD RILGLGD GA G+GIPVGKL LYT +G + P Q LP+ +D+GT
Sbjct: 149 NYPRQDIRCIVVTDAGRILGLGDLGASGLGIPVGKLMLYTLIGQVDPDQTLPVQLDMGTD 208
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L DPLY G R RV
Sbjct: 209 RKEILADPLYHGWRHPRV 226
Score = 67.3 bits (157), Expect = 3e-10
Identities = 34/77 (44%), Positives = 48/77 (62%), Gaps = 1/77 (1%)
Frame = +2
Query: 44 PLNKYIYLMGLLDRNEHLFYRFVADNVAE-MMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
PL KYI+L ++N F+RF+ + E MPI+YTPTVG ACQK+ + RG++IT
Sbjct: 77 PLLKYIFLANEREKNSQSFWRFLFTHPPEETMPILYTPTVGEACQKWATHRQSYRGIYIT 136
Query: 221 IHDKGHVYDVLRTGRRR 271
D G + D+LR R+
Sbjct: 137 PEDSGKIKDILRNYPRQ 153
Score = 62.1 bits (144), Expect = 1e-08
Identities = 30/59 (50%), Positives = 39/59 (66%)
Frame = +2
Query: 506 RLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ + EF+ AV +G CLVQFEDF AF+LL+ + + FNDDI+GTAAVA A L
Sbjct: 233 KFVTEFVEAVKEVFGDTCLVQFEDFEMETAFKLLDHFRWRCNCFNDDIEGTAAVAAATL 291
>UniRef50_P37221 Cluster: NAD-dependent malic enzyme 62 kDa isoform,
mitochondrial precursor; n=41; Eukaryota|Rep:
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor - Solanum tuberosum (Potato)
Length = 626
Score = 99 bits (238), Expect = 5e-20
Identities = 45/78 (57%), Positives = 55/78 (70%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP V IVVTDG RILGLGD G G+GI +GKL LY A GI P + LP+ IDVGT
Sbjct: 183 NWPADQVDMIVVTDGSRILGLGDLGIQGIGIAIGKLDLYVAAAGINPQRVLPVMIDVGTD 242
Query: 436 TQSMLDDPLYIGLRQRRV 489
+++L DPLY+GL+ R+
Sbjct: 243 NENLLKDPLYLGLQDHRL 260
Score = 76.6 bits (180), Expect = 5e-13
Identities = 34/87 (39%), Positives = 55/87 (63%), Gaps = 5/87 (5%)
Frame = +2
Query: 8 ELCKLSIDTYENPLNKYIY-----LMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLAC 172
+L +L + + P + Y+ L L DRNE L+Y+ + +N+ E PIVYTPTVGL C
Sbjct: 95 DLKRLEVQARDGPSDPYVLAKWRILNRLHDRNETLYYKVLMENIEEYAPIVYTPTVGLVC 154
Query: 173 QKFGLVYRRPRGLFITIHDKGHVYDVL 253
QK+ ++RRPRG++ + D+G + ++
Sbjct: 155 QKYSGLFRRPRGMYFSAEDRGEMMSMV 181
Score = 70.9 bits (166), Expect = 3e-11
Identities = 34/58 (58%), Positives = 44/58 (75%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++DEFM AV R+ + +VQFEDF + AF+LL++Y N Y FNDDIQGTA VA+AGL
Sbjct: 268 VIDEFMEAVFTRW-PHVIVQFEDFQSKWAFKLLQRYRNNYRMFNDDIQGTAGVAIAGL 324
>UniRef50_Q5K758 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 600
Score = 98.7 bits (235), Expect = 1e-19
Identities = 44/69 (63%), Positives = 54/69 (78%)
Frame = +1
Query: 283 IVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLDDPL 462
+VVTDG RILGLGD G GMGI VGKL LY A GG+ PH CLP+ +D+GT +++ +DPL
Sbjct: 187 LVVTDGSRILGLGDLGIGGMGISVGKLNLYVAGGGVNPHGCLPVVLDMGTNNEAVRNDPL 246
Query: 463 YIGLRQRRV 489
YIGL+Q RV
Sbjct: 247 YIGLKQPRV 255
Score = 73.7 bits (173), Expect = 4e-12
Identities = 32/89 (35%), Positives = 51/89 (57%)
Frame = +2
Query: 17 KLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYR 196
K + PL K+++L + + +LF+ + D + E+ PIVYTPTVG ACQK+ +Y
Sbjct: 95 KAHLQNLPTPLLKHVHLSKIRREDPNLFFSVMRDELTELAPIVYTPTVGEACQKYSQIYS 154
Query: 197 RPRGLFITIHDKGHVYDVLRTGRRRTSAP 283
P GL++ I DK + ++L + AP
Sbjct: 155 GPEGLYLNIEDKDRIPEILHQYASKLVAP 183
Score = 49.2 bits (112), Expect = 9e-05
Identities = 24/56 (42%), Positives = 34/56 (60%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
+D FM A + + ++Q EDF + AF L +Y KY FNDDI+GT +V +AG
Sbjct: 264 MDAFMAAASEAFPK-AVIQHEDFYSEAAFDFLARYKEKYRMFNDDIEGTGSVILAG 318
>UniRef50_A4RZU1 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 539
Score = 95.9 bits (228), Expect = 8e-19
Identities = 44/72 (61%), Positives = 52/72 (72%)
Frame = +1
Query: 274 VRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
V VVTDGERILGLGD GA GMGI GK+ LYT G++P CLP+ +DVGT Q +LD
Sbjct: 124 VAVAVVTDGERILGLGDLGAGGMGISEGKILLYTVCAGVRPSACLPVCLDVGTNNQRLLD 183
Query: 454 DPLYIGLRQRRV 489
DP Y GLR+ R+
Sbjct: 184 DPNYKGLRRTRL 195
Score = 78.2 bits (184), Expect = 2e-13
Identities = 38/58 (65%), Positives = 44/58 (75%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+DEFMR + R + CLVQFEDFGNANAFR+LEKY FNDDIQGTAA+ +A L
Sbjct: 203 LVDEFMREM-RAWQPRCLVQFEDFGNANAFRILEKYRTTGPCFNDDIQGTAAITLAAL 259
Score = 62.1 bits (144), Expect = 1e-08
Identities = 32/80 (40%), Positives = 44/80 (55%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
+PL +Y+YL L + FYR + E+MP VYTPTVG AC+K+ + G++IT
Sbjct: 16 DPLERYVYLRELQRASAETFYRALVREPLELMPFVYTPTVGEACEKYHRLGIETNGVYIT 75
Query: 221 IHDKGHVYDVLRTGRRRTSA 280
D G V LR R +A
Sbjct: 76 ADDAGRVGAKLRGHWDRAAA 95
>UniRef50_Q5CS07 Cluster: Malic enzyme; n=2; Cryptosporidium|Rep:
Malic enzyme - Cryptosporidium parvum Iowa II
Length = 614
Score = 94.3 bits (224), Expect = 2e-18
Identities = 41/73 (56%), Positives = 55/73 (75%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D+ IV+TDG RILGLGD G GMGIP+GKL+LY LGGI P + LPI++D+GT T +L
Sbjct: 199 DIEIIVLTDGGRILGLGDLGLNGMGIPMGKLSLYITLGGIDPSKVLPISLDIGTNTNDIL 258
Query: 451 DDPLYIGLRQRRV 489
D Y+G+ ++R+
Sbjct: 259 SDKYYLGICEKRI 271
Score = 72.5 bits (170), Expect = 9e-12
Identities = 64/240 (26%), Positives = 109/240 (45%), Gaps = 13/240 (5%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV +I+ ++ + KY +L + + LF+ + ++ P+VYTPTVG C +F
Sbjct: 105 QVSRLWTAINKIDSNIGKYTFLENIRSSSFILFHSLLDKYFKDLTPLVYTPTVGEGCIEF 164
Query: 182 GL--VYRR--PRGLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWAS 349
R GL++ KG +Y++L+ + ++ T+ G
Sbjct: 165 SRNPTIRNWLGSGLYLNKSHKGRIYEILKDFKS-DDIEIIVLTDG-------GRILGLGD 216
Query: 350 LWANSRSTPRSEASSLIS---VYPSLSTWVRSPSRCWTTRCTSGSGS-GACWPRLRR--- 508
L N P + S I+ + PS + T S G C R+
Sbjct: 217 LGLNGMGIPMGKLSLYITLGGIDPSKVLPISLDIGTNTNDILSDKYYLGICEKRIDNDEY 276
Query: 509 --LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+DE +A+ +R+ N ++Q+ED + A +L+ Y +K+ FNDDIQGTA++ +AGL
Sbjct: 277 FPLMDEITKAIFKRW-PNTVLQWEDLTTSRAIDILDIYQDKFRCFNDDIQGTASIVLAGL 335
>UniRef50_A5C6I9 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 498
Score = 93.9 bits (223), Expect = 3e-18
Identities = 43/72 (59%), Positives = 53/72 (73%)
Frame = +1
Query: 274 VRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
V IV+TDG RILGLGD G G+GIP+GKL +Y A GI P + LPI +DVGT Q +L+
Sbjct: 41 VDMIVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRILPIMLDVGTNNQRLLE 100
Query: 454 DPLYIGLRQRRV 489
D LY+GLRQ R+
Sbjct: 101 DRLYLGLRQPRL 112
Score = 56.0 bits (129), Expect = 8e-07
Identities = 25/39 (64%), Positives = 29/39 (74%)
Frame = +2
Query: 566 QFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
QFEDF AF L++Y K+C FNDDIQGTA VA+AGL
Sbjct: 167 QFEDFQMKWAFETLQRYRKKFCMFNDDIQGTAGVALAGL 205
>UniRef50_A1SVL3 Cluster: Malic enzyme aka malate dehydrogenase
(Oxaloacetate-decarboxylating) (NADP(+)); n=4;
Gammaproteobacteria|Rep: Malic enzyme aka malate
dehydrogenase (Oxaloacetate-decarboxylating) (NADP(+)) -
Psychromonas ingrahamii (strain 37)
Length = 571
Score = 93.5 bits (222), Expect = 4e-18
Identities = 39/73 (53%), Positives = 55/73 (75%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D++ IVVTD + ILG+GD G GMGIP+GKL+LYT GI P C+PI +D+GT Q +L
Sbjct: 153 DIQIIVVTDSQGILGIGDQGVGGMGIPIGKLSLYTLGAGIHPDHCMPIALDIGTDNQDLL 212
Query: 451 DDPLYIGLRQRRV 489
DP+Y+G+ ++R+
Sbjct: 213 ADPMYLGIPKKRL 225
Score = 74.5 bits (175), Expect = 2e-12
Identities = 35/66 (53%), Positives = 45/66 (68%), Gaps = 3/66 (4%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT-- 220
+ KY YL L DRNE LFY ++ N+ EM PI+YTPTVG ACQ+F ++ RGL++T
Sbjct: 78 IEKYQYLRALQDRNETLFYALISRNIEEMTPIIYTPTVGKACQEFSHRFQIARGLYLTTD 137
Query: 221 -IHDKG 235
IHD G
Sbjct: 138 NIHDVG 143
Score = 48.4 bits (110), Expect = 2e-04
Identities = 21/49 (42%), Positives = 33/49 (67%)
Frame = +2
Query: 536 VRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
V+ + ++Q+EDF +NAF L Y ++ +FNDD+QGT AV +AG+
Sbjct: 241 VKLHFPKAVLQWEDFSKSNAFDNLSDYEDELPSFNDDVQGTGAVVLAGI 289
>UniRef50_A6XP71 Cluster: Malic enzyme protein 2; n=2;
Mucoromycotina|Rep: Malic enzyme protein 2 - Mortierella
alpina (Mortierella renispora)
Length = 669
Score = 91.1 bits (216), Expect = 2e-17
Identities = 42/75 (56%), Positives = 51/75 (68%)
Frame = +1
Query: 262 PETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQ 441
P + V+TDG RILGLGD G GMGIPVGKL LY A GI P + LPIT+D+GT +
Sbjct: 235 PSLTPQIAVITDGSRILGLGDLGVGGMGIPVGKLQLYVAGAGIDPRRTLPITLDLGTNNE 294
Query: 442 SMLDDPLYIGLRQRR 486
L D Y+GLR++R
Sbjct: 295 DKLKDEFYLGLREKR 309
Score = 64.5 bits (150), Expect = 2e-09
Identities = 31/89 (34%), Positives = 51/89 (57%), Gaps = 6/89 (6%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVY------RRPRG 208
+ KY++L L + N LFY V D + E +P++YTPTVG ACQ + +Y +P G
Sbjct: 151 IEKYVFLAWLRNTNVRLFYGLVGDQLEETLPLIYTPTVGTACQNYSSIYPFLAPPGQPDG 210
Query: 209 LFITIHDKGHVYDVLRTGRRRTSAPLL*P 295
LF++I+D ++ +++ + P L P
Sbjct: 211 LFLSINDLPNLTQIIQNYKPFPQDPSLTP 239
Score = 56.8 bits (131), Expect = 5e-07
Identities = 28/56 (50%), Positives = 38/56 (67%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
+D + A+ Y + L+QFEDF + +AF+LL+KY K FNDDIQGT AV +AG
Sbjct: 319 VDAVIEALTSLYPK-LLIQFEDFSSEHAFQLLDKYQPKKFCFNDDIQGTGAVILAG 373
>UniRef50_A4SKB8 Cluster: NAD-dependent malic enzyme; n=2;
Aeromonas|Rep: NAD-dependent malic enzyme - Aeromonas
salmonicida (strain A449)
Length = 516
Score = 90.2 bits (214), Expect = 4e-17
Identities = 41/75 (54%), Positives = 54/75 (72%)
Frame = +1
Query: 265 ETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQS 444
E +V IV++DGER+LGLGD G GMGI +GKLALY+A GGI P + LP+ +DVGT
Sbjct: 121 EQEVDVIVISDGERVLGLGDLGIGGMGICIGKLALYSAAGGINPARTLPLCVDVGTNNPE 180
Query: 445 MLDDPLYIGLRQRRV 489
+L+D Y+G + RV
Sbjct: 181 LLEDDSYLGWQANRV 195
Score = 89.8 bits (213), Expect = 5e-17
Identities = 55/217 (25%), Positives = 100/217 (46%)
Frame = +2
Query: 26 IDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPR 205
+ ++P ++++ L L + N LFY V ++ E++PI+YTP VG ACQ+ +Y R
Sbjct: 42 VSAMQSPFDQHLLLRQLQEDNPVLFYDLVRHHLPELLPIIYTPVVGEACQRHSDLYLRSH 101
Query: 206 GLFITIHDKGHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWANSRSTPRSE 385
GL+++ HD+ + + + ++ E V + + + +
Sbjct: 102 GLYLSWHDRDDLDAIFAAVEQEVDVIVISDGERVLGLGDLGIGGMGICIGKLALYSAAGG 161
Query: 386 ASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWPRLRRLLDEFMRAVVRRYGQNCLV 565
+ ++ + +P + +D+ + A+ R+ Q ++
Sbjct: 162 INPARTLPLCVDVGTNNPELLEDDSYLGWQANRVDGESYYHFMDKVVAAIRTRWPQ-VVL 220
Query: 566 QFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
QFEDF +A LL +Y ++ C FNDDIQGTAAVA A
Sbjct: 221 QFEDFAGKHAANLLARYRDELCMFNDDIQGTAAVASA 257
>UniRef50_Q4PC56 Cluster: Malic enzyme; n=1; Ustilago maydis|Rep:
Malic enzyme - Ustilago maydis (Smut fungus)
Length = 634
Score = 89.4 bits (212), Expect = 7e-17
Identities = 43/79 (54%), Positives = 52/79 (65%), Gaps = 2/79 (2%)
Frame = +1
Query: 256 NWPET--DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVG 429
NWP R V+TDG RILGLGD G G GI +GKL+LY A GI P LPI +D+G
Sbjct: 201 NWPVPAGSPRIAVITDGSRILGLGDLGWNGQGISIGKLSLYVAGAGIHPRATLPIVVDLG 260
Query: 430 TITQSMLDDPLYIGLRQRR 486
T + L+DPLY+GLR+ R
Sbjct: 261 TNNKKNLEDPLYLGLRRER 279
Score = 77.8 bits (183), Expect = 2e-13
Identities = 32/70 (45%), Positives = 46/70 (65%)
Frame = +2
Query: 44 PLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 223
PL K++ L L N L+Y + N E++P++YTPTVG ACQKF +YRRP GL I++
Sbjct: 130 PLEKHVMLASLRQTNTRLYYATILANKEEILPLIYTPTVGEACQKFSHIYRRPEGLSISL 189
Query: 224 HDKGHVYDVL 253
DKG + ++
Sbjct: 190 EDKGKIASIV 199
Score = 68.5 bits (160), Expect = 1e-10
Identities = 32/56 (57%), Positives = 40/56 (71%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAG 679
+DE M A+ RY N ++QFEDF + NAFR LE+Y KY FNDDIQGT +V +AG
Sbjct: 289 MDEVMHALHTRY-PNLIIQFEDFTSENAFRFLERYQGKYPMFNDDIQGTGSVILAG 343
>UniRef50_Q4T5P0 Cluster: Malic enzyme; n=1; Tetraodon
nigroviridis|Rep: Malic enzyme - Tetraodon nigroviridis
(Green puffer)
Length = 306
Score = 87.4 bits (207), Expect = 3e-16
Identities = 38/47 (80%), Positives = 41/47 (87%)
Frame = +2
Query: 542 RYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
RYG +CL+QFEDF N NAFRLL KY + YCTFNDDIQGTAAVAVAGL
Sbjct: 1 RYGMDCLIQFEDFANINAFRLLSKYRDMYCTFNDDIQGTAAVAVAGL 47
>UniRef50_P45868 Cluster: Probable NAD-dependent malic enzyme 2;
n=37; Bacteria|Rep: Probable NAD-dependent malic enzyme
2 - Bacillus subtilis
Length = 582
Score = 87.4 bits (207), Expect = 3e-16
Identities = 38/73 (52%), Positives = 52/73 (71%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D+ IV TD E ILG+GD+G G+ I +GKLA+YTA GI P + +P+ +DVGT + +L
Sbjct: 166 DIDLIVATDSESILGIGDWGVGGINIAIGKLAVYTAAAGIDPSRVIPVVLDVGTNNEKLL 225
Query: 451 DDPLYIGLRQRRV 489
+DPLYIG + RV
Sbjct: 226 NDPLYIGNKHERV 238
Score = 68.5 bits (160), Expect = 1e-10
Identities = 28/59 (47%), Positives = 45/59 (76%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITI 223
L + +YL L +RNE LFY+ + +++ EM+P+VYTPTVG A Q++ YRRP+G++++I
Sbjct: 89 LRQNVYLSDLANRNEVLFYKLLKNHLREMLPVVYTPTVGEAIQEYSHEYRRPQGIYLSI 147
Score = 56.0 bits (129), Expect = 8e-07
Identities = 24/62 (38%), Positives = 42/62 (67%)
Frame = +2
Query: 497 RLRRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
R +D +++A ++ + + L+ +ED GN NA +++KY ++ TFNDDIQGT A+ +A
Sbjct: 242 RYEAFIDAYVKAALKFFPK-ALLHWEDLGNKNARNIMKKYNHEILTFNDDIQGTGAITLA 300
Query: 677 GL 682
G+
Sbjct: 301 GV 302
>UniRef50_Q5KBK5 Cluster: Nad-dependent malic enzyme, putative; n=2;
Filobasidiella neoformans|Rep: Nad-dependent malic
enzyme, putative - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 584
Score = 85.8 bits (203), Expect = 9e-16
Identities = 37/73 (50%), Positives = 54/73 (73%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
++ IVV+DGE ILG+GD G+ G+GI K +Y+ + G+ P +CL IT+DVGT Q +L
Sbjct: 158 ELELIVVSDGEAILGIGDQGSGGIGISGAKAVIYSLIAGVDPAKCLAITLDVGTNNQDLL 217
Query: 451 DDPLYIGLRQRRV 489
+DPLYIG R++R+
Sbjct: 218 NDPLYIGYREKRL 230
Score = 45.6 bits (103), Expect = 0.001
Identities = 22/61 (36%), Positives = 34/61 (55%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
E + K +L + +N LFY + ++ EM PIVYTPT A + ++RR GL++
Sbjct: 79 ETNILKNSFLQSMKAQNWTLFYALLQAHLVEMFPIVYTPTEADAIADYSHLFRRSEGLYL 138
Query: 218 T 220
T
Sbjct: 139 T 139
Score = 35.5 bits (78), Expect = 1.2
Identities = 21/62 (33%), Positives = 35/62 (56%)
Frame = +2
Query: 497 RLRRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
R + +D+F+ +V+++ CL+ FED + L + + FNDDIQGT AV +A
Sbjct: 234 RYDQFVDKFV-GLVKKHQPECLLHFEDLVSP---MLNDSSEDIGARFNDDIQGTGAVTLA 289
Query: 677 GL 682
+
Sbjct: 290 AV 291
>UniRef50_Q9RYN4 Cluster: Malate oxidoreductase; n=6;
Deinococci|Rep: Malate oxidoreductase - Deinococcus
radiodurans
Length = 580
Score = 83.8 bits (198), Expect = 3e-15
Identities = 38/78 (48%), Positives = 51/78 (65%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
N P DVR IV TD ILG+GD G GM I +GKL+LYT GG+ P + LP+ +DVGT
Sbjct: 161 NVPLNDVRIIVATDSSAILGIGDQGFGGMAISIGKLSLYTVAGGVGPDKTLPVELDVGTG 220
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +DP Y+G++ R+
Sbjct: 221 RADLREDPHYLGVKHERL 238
Score = 73.3 bits (172), Expect = 5e-12
Identities = 33/58 (56%), Positives = 43/58 (74%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
L K++YL L DRNE LFY ++ +V EM+PIVYTPTVG A +KF +YR PRGL ++
Sbjct: 91 LEKHVYLRNLQDRNEVLFYALLSHHVEEMLPIVYTPTVGDAVKKFSQIYRYPRGLTLS 148
Score = 56.0 bits (129), Expect = 8e-07
Identities = 25/57 (43%), Positives = 39/57 (68%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+D+F+ A ++RY + ++Q+EDF AF +L +Y +FNDDIQGT AV +AG+
Sbjct: 247 IDKFVEATIQRYPK-AIIQWEDFSKDAAFEVLARYRKVVPSFNDDIQGTGAVVLAGV 302
>UniRef50_Q6AL43 Cluster: Related to NAD-dependent malic enzyme;
n=1; Desulfotalea psychrophila|Rep: Related to
NAD-dependent malic enzyme - Desulfotalea psychrophila
Length = 578
Score = 83.4 bits (197), Expect = 5e-15
Identities = 38/74 (51%), Positives = 53/74 (71%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D+R VVTD + ILG+GD GA G+ I +GKL LYT GI P CLPI++D+GT +++L
Sbjct: 154 DIRVAVVTDNQGILGIGDQGAGGIAICLGKLMLYTQGAGIAPWHCLPISLDIGTDNEALL 213
Query: 451 DDPLYIGLRQRRVL 492
D Y+G RQ+R++
Sbjct: 214 ADKHYLGWRQKRLV 227
Score = 66.1 bits (154), Expect = 7e-10
Identities = 31/85 (36%), Positives = 48/85 (56%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE C + + E+ KYI++ L DRN L + + ++ + M I+YTPTVGLA QK+
Sbjct: 64 QVENCHIKLGEKESEEEKYIFIRSLFDRNVTLAHALIQSDLEKFMGIIYTPTVGLAVQKY 123
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLR 256
++R+ GL + D+LR
Sbjct: 124 SAMFRQANGLHFSPDTIDQAEDILR 148
Score = 52.4 bits (120), Expect = 1e-05
Identities = 24/57 (42%), Positives = 34/57 (59%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+D F RA R N L Q+EDF NAF + +KY + +FNDD+QGT A+ + +
Sbjct: 235 IDRFARAF-RNVFPNALCQWEDFSKQNAFDIRDKYRHSMVSFNDDVQGTGAITFSAI 290
>UniRef50_Q7SHJ8 Cluster: Malic enzyme; n=12; Pezizomycotina|Rep:
Malic enzyme - Neurospora crassa
Length = 611
Score = 83.0 bits (196), Expect = 6e-15
Identities = 40/75 (53%), Positives = 52/75 (69%)
Frame = +1
Query: 262 PETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQ 441
PE D+ IVVTDGE ILG+GD G G+ I + KL L T GGI P++ LP+ +D GT +
Sbjct: 163 PE-DIDYIVVTDGEEILGIGDQGCGGILISIAKLVLMTICGGIHPNRVLPVVLDCGTNNE 221
Query: 442 SMLDDPLYIGLRQRR 486
+L D LY+GLRQ+R
Sbjct: 222 ELLKDDLYLGLRQKR 236
Score = 64.1 bits (149), Expect = 3e-09
Identities = 28/67 (41%), Positives = 43/67 (64%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
N L K ++ + ++NE LFYR + D++ EM +VYTPT G A Q + ++RRP G+F+
Sbjct: 87 NDLAKNTFMTSMKEQNEVLFYRLLHDHLDEMFSVVYTPTEGEAIQNYSRLFRRPEGVFLN 146
Query: 221 IHDKGHV 241
I+D V
Sbjct: 147 INDMDSV 153
Score = 49.2 bits (112), Expect = 9e-05
Identities = 24/57 (42%), Positives = 35/57 (61%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++ F++A Y + + FEDFG NA RLLE+Y + FNDD+QGT V +A +
Sbjct: 246 VETFIKAARELYPR-AYIHFEDFGLDNARRLLERYRPQMPCFNDDVQGTGCVTLAAI 301
>UniRef50_Q5KEY3 Cluster: Malic enzyme; n=1; Filobasidiella
neoformans|Rep: Malic enzyme - Cryptococcus neoformans
(Filobasidiella neoformans)
Length = 629
Score = 82.2 bits (194), Expect = 1e-14
Identities = 70/232 (30%), Positives = 109/232 (46%), Gaps = 22/232 (9%)
Frame = +2
Query: 53 KYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIHDK 232
K+ +L L D+N+ LFYR + D + E++ ++YTP A + ++RRP G +I+ ++
Sbjct: 108 KHAFLASLRDQNQVLFYRLMQDRLKELLGVLYTPGAAEAVAGYSSLFRRPVGCYISFPNQ 167
Query: 233 --------GHVYDVLRTGRRRTSAPLL*PTENVFWAWAISARAGWASLWANSRSTPRSEA 388
GH+ DV RT + P + + A A L +
Sbjct: 168 DGMRAQLEGHLTDVNRTADVAYDSNK--PDDAIDLVVVTDAEA---ILGIGDQGVGGITI 222
Query: 389 SSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGAC--------WPRLR---RLLDEFMRAV 535
S+ + +L + +P+R G+ + A W R R + D+F+
Sbjct: 223 STSKAALYTLGAGI-NPNRILPVVLDCGTDNHALFSDPLYMGWKRTRIRGKNYDQFIDRF 281
Query: 536 V---RRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ R N ++ FEDFG ANA+RL+EKY N FNDDIQGT AVA+A L
Sbjct: 282 ITNCRELFPNAIIHFEDFGMANAYRLMEKYKN-IPMFNDDIQGTGAVALAAL 332
Score = 76.2 bits (179), Expect = 7e-13
Identities = 34/78 (43%), Positives = 49/78 (62%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
N P+ + +VVTD E ILG+GD G G+ I K ALYT GI P++ LP+ +D GT
Sbjct: 192 NKPDDAIDLVVVTDAEAILGIGDQGVGGITISTSKAALYTLGAGINPNRILPVVLDCGTD 251
Query: 436 TQSMLDDPLYIGLRQRRV 489
++ DPLY+G ++ R+
Sbjct: 252 NHALFSDPLYMGWKRTRI 269
>UniRef50_Q8Y5Y8 Cluster: Lmo1915 protein; n=15; Firmicutes|Rep:
Lmo1915 protein - Listeria monocytogenes
Length = 547
Score = 81.4 bits (192), Expect = 2e-14
Identities = 37/78 (47%), Positives = 55/78 (70%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
N P D+ IV+TDGE +LG+GD+ G+ I VGKLA+YT G+ P + LP+ ID GT
Sbjct: 135 NNPNIDM--IVITDGEGVLGIGDWSVNGVKIAVGKLAVYTVAAGLAPDRVLPVVIDAGTN 192
Query: 436 TQSMLDDPLYIGLRQRRV 489
+++L+DPLY+G ++ R+
Sbjct: 193 NETLLNDPLYLGNKRPRL 210
Score = 58.4 bits (135), Expect = 1e-07
Identities = 25/50 (50%), Positives = 34/50 (68%)
Frame = +2
Query: 533 VVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
V++ ++ +EDFG ANA R+L Y +K CTFNDDIQGT A+ VA +
Sbjct: 225 VMKEVFPKAILHWEDFGRANASRILHNYRDKICTFNDDIQGTGAMVVAAV 274
Score = 51.2 bits (117), Expect = 2e-05
Identities = 25/65 (38%), Positives = 37/65 (56%), Gaps = 1/65 (1%)
Frame = +2
Query: 26 IDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP- 202
I+ E PL+K+ L L + N L+Y V NV + +PI+YTPT+G A ++ Y P
Sbjct: 53 IENLETPLHKHRLLTNLYNENRTLYYYVVTKNVTDYLPIIYTPTIGDAVIQYHKDYTAPD 112
Query: 203 RGLFI 217
LF+
Sbjct: 113 EALFV 117
>UniRef50_A4RQC9 Cluster: Malic enzyme; n=2; Ostreococcus|Rep: Malic
enzyme - Ostreococcus lucimarinus CCE9901
Length = 549
Score = 80.6 bits (190), Expect = 3e-14
Identities = 35/78 (44%), Positives = 49/78 (62%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP + IVVTDG R+LGLGD G GM I VGK++LY A GG P + +P+ +D+GT
Sbjct: 133 NWPSPETEIIVVTDGGRVLGLGDLGTNGMAISVGKVSLYVASGGFDPAKSMPVCLDLGTS 192
Query: 436 TQSMLDDPLYIGLRQRRV 489
++ Y+G + R+
Sbjct: 193 NVALRAHDFYLGADEPRL 210
Score = 70.9 bits (166), Expect = 3e-11
Identities = 32/78 (41%), Positives = 49/78 (62%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ + +E +N+YI+L L +NE LFYR + +++ ++MPIVYTPTVG AC F
Sbjct: 48 QVKRAWTQLCAFEEDMNRYIFLENLHMQNERLFYRVLVEHLEDLMPIVYTPTVGEACINF 107
Query: 182 GLVYRRPRGLFITIHDKG 235
+YR G++ + D G
Sbjct: 108 DALYRNRCGMYFSRLDSG 125
Score = 59.7 bits (138), Expect = 6e-08
Identities = 29/58 (50%), Positives = 39/58 (67%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++ EF AV ++ NCL+QFEDF +AFR+L+ +K FNDDIQGT A +AGL
Sbjct: 218 VVTEFCLAVKDKW-PNCLIQFEDFKTEDAFRILDHMRHKVLCFNDDIQGTGATILAGL 274
>UniRef50_A0Q531 Cluster: NAD-dependent malic enzyme; n=10;
Francisella tularensis|Rep: NAD-dependent malic enzyme -
Francisella tularensis subsp. novicida (strain U112)
Length = 604
Score = 80.2 bits (189), Expect = 4e-14
Identities = 37/76 (48%), Positives = 50/76 (65%)
Frame = +2
Query: 26 IDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPR 205
+D N L KY++L L D N LFY FV +N+ E+MPI+YTPTVG A QK+ +R+
Sbjct: 74 LDLKPNSLEKYVFLNRLHDLNTTLFYHFVRENLEEIMPIIYTPTVGEAVQKYSSSFRKQS 133
Query: 206 GLFITIHDKGHVYDVL 253
GLFI+I K H+ +L
Sbjct: 134 GLFISISHKKHIARIL 149
Score = 74.1 bits (174), Expect = 3e-12
Identities = 32/69 (46%), Positives = 48/69 (69%)
Frame = +1
Query: 283 IVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLDDPL 462
++VTDGE +LG+GD G GM I +GK+ +Y A GI P + LP+ +D+GT ++L+ P
Sbjct: 160 VLVTDGEAVLGIGDQGIGGMNISIGKIMVYVAASGIDPARVLPVQLDMGTNNDALLNAPG 219
Query: 463 YIGLRQRRV 489
Y+G+R RV
Sbjct: 220 YLGVRLPRV 228
Score = 60.1 bits (139), Expect = 5e-08
Identities = 28/55 (50%), Positives = 37/55 (67%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
++EF+ V R+ N + +ED G NA R+LEKY +K CTFNDDI+GT VA A
Sbjct: 237 IEEFVTQVKARF-PNVFLHWEDLGRDNATRILEKYKDKLCTFNDDIEGTGIVATA 290
>UniRef50_Q2HCG7 Cluster: Malic enzyme; n=1; Chaetomium
globosum|Rep: Malic enzyme - Chaetomium globosum (Soil
fungus)
Length = 586
Score = 78.2 bits (184), Expect = 2e-13
Identities = 35/65 (53%), Positives = 48/65 (73%)
Frame = +1
Query: 295 DGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLDDPLYIGL 474
DGE ILG+GD G G+ I V KLAL T GI P++ LP+ +D GT +++L+DPLY+GL
Sbjct: 182 DGEEILGIGDQGCGGILISVAKLALMTLCAGIHPNRVLPVVLDCGTDNETLLNDPLYLGL 241
Query: 475 RQRRV 489
R++RV
Sbjct: 242 REKRV 246
Score = 47.6 bits (108), Expect = 3e-04
Identities = 23/66 (34%), Positives = 39/66 (59%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
L K +L + ++NE L+++ M +VYTPT G A + F ++RRP+G+F+ +H
Sbjct: 87 LAKNTFLTSMKEQNEVLYFK--------MFSVVYTPTEGDAIENFSRLFRRPQGVFLNVH 138
Query: 227 DKGHVY 244
D V+
Sbjct: 139 DCDRVH 144
>UniRef50_A2QY66 Cluster: Malic enzyme; n=2; cellular organisms|Rep:
Malic enzyme - Aspergillus niger
Length = 609
Score = 77.0 bits (181), Expect = 4e-13
Identities = 39/73 (53%), Positives = 49/73 (67%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
+V IVV+DGE ILG+GD G + I V KLAL T GI P + LP+ +D GT +S+L
Sbjct: 187 EVDYIVVSDGEEILGIGDQGVGAILISVAKLALTTLCAGIHPSRQLPVVLDCGTDNESLL 246
Query: 451 DDPLYIGLRQRRV 489
D LY+GLRQ RV
Sbjct: 247 TDELYLGLRQSRV 259
Score = 60.1 bits (139), Expect = 5e-08
Identities = 26/84 (30%), Positives = 48/84 (57%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ + +N L K ++ + +NE L+Y+ + ++ EM+ I+YTPT G A Q +
Sbjct: 96 QVQRAYEQYSSRDNDLAKNTFMASMKAQNEVLYYKLIDTHLKEMLSIIYTPTEGDAIQNY 155
Query: 182 GLVYRRPRGLFITIHDKGHVYDVL 253
++R+P G F+ I D+ + + L
Sbjct: 156 SRLFRKPEGCFLNIRDQDRIEECL 179
Score = 50.4 bits (115), Expect = 4e-05
Identities = 23/59 (38%), Positives = 38/59 (64%)
Frame = +2
Query: 506 RLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ +++F+ +R+ + + FEDFG NA R+L+K+ ++ FNDDIQGT V +A L
Sbjct: 266 QFVEKFVETARKRFPR-AYIHFEDFGLHNAKRILDKFKSRIPCFNDDIQGTGCVTLAAL 323
>UniRef50_Q95061 Cluster: Malic enzyme; n=2; Giardia
intestinalis|Rep: Malic enzyme - Giardia lamblia
(Giardia intestinalis)
Length = 557
Score = 76.6 bits (180), Expect = 5e-13
Identities = 38/76 (50%), Positives = 47/76 (61%)
Frame = +1
Query: 259 WPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTIT 438
WP + R IV TDG RILGLGD G G I VGKL LY+ GG P LPI+ D G T
Sbjct: 131 WPYSP-RIIVATDGTRILGLGDLGTGGHQICVGKLTLYSLGGGFAPEHTLPISFDFGCNT 189
Query: 439 QSMLDDPLYIGLRQRR 486
+ +DP Y+G+ ++R
Sbjct: 190 DKIREDPHYLGIPEKR 205
Score = 65.3 bits (152), Expect = 1e-09
Identities = 31/84 (36%), Positives = 46/84 (54%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q+ C+ D P K++YL L + NE LF F + E++PIVYTPTVG AC +
Sbjct: 44 QISRCRAQFDVLTTPTEKWLYLTRLQEVNETLFSGFCLKYLKEVLPIVYTPTVGTACSNY 103
Query: 182 GLVYR-RPRGLFITIHDKGHVYDV 250
L+++ PRG ++ G V +
Sbjct: 104 SLLWQGYPRGFYLNRTHLGKVKQI 127
Score = 52.0 bits (119), Expect = 1e-05
Identities = 25/56 (44%), Positives = 36/56 (64%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
++ E + AV ++ +C+ QFEDF N AF LL+K+ + FNDDIQGT +A A
Sbjct: 214 IIKETIEAVHSKW-PDCIFQFEDFSNDTAFTLLKKHRDAGPVFNDDIQGTGCIAAA 268
>UniRef50_Q48796 Cluster: Malolactic enzyme; n=49; Bacteria|Rep:
Malolactic enzyme - Oenococcus oeni (Leuconostoc oenos)
Length = 541
Score = 76.6 bits (180), Expect = 5e-13
Identities = 34/73 (46%), Positives = 48/73 (65%)
Frame = +1
Query: 271 DVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSML 450
D++ +VV+D E ILG+GD+G G+ I VGKL +YT GI P L + ID GT + +L
Sbjct: 135 DIKLLVVSDAEGILGIGDWGVQGVDIAVGKLMVYTVAAGIDPSTVLAVVIDAGTNNEKLL 194
Query: 451 DDPLYIGLRQRRV 489
DP+Y+G + RV
Sbjct: 195 KDPMYLGNKFNRV 207
Score = 52.0 bits (119), Expect = 1e-05
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = +2
Query: 554 NCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
N + +EDFG +NA +L Y +K TFNDDIQGT V +AG+
Sbjct: 229 NLYLHWEDFGRSNASNILNSYKDKIATFNDDIQGTGIVVLAGV 271
Score = 50.0 bits (114), Expect = 5e-05
Identities = 26/97 (26%), Positives = 47/97 (48%), Gaps = 1/97 (1%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ + + L K ++LM + + N LFY+ + +V E MPIVY PT+ + +
Sbjct: 43 QVDQTYAQFQSKVSNLEKRLFLMEIFNTNHVLFYKLFSQHVVEFMPIVYDPTIADTIENY 102
Query: 182 GLVYRRPRG-LFITIHDKGHVYDVLRTGRRRTSAPLL 289
++ P+G F+ I+ ++ L+ LL
Sbjct: 103 SELFVEPQGAAFLDINHPENIQSTLKNAANGRDIKLL 139
>UniRef50_Q7K3R0 Cluster: Malic enzyme; n=2; Sophophora|Rep: Malic
enzyme - Drosophila melanogaster (Fruit fly)
Length = 633
Score = 72.5 bits (170), Expect = 9e-12
Identities = 33/77 (42%), Positives = 46/77 (59%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP VR ++V++G +L +GD G M I L GGI P CL + +DVGT
Sbjct: 196 NWPFRCVRCLLVSNGASVLSVGDLGVDEMPILFSNLHQNVVYGGIHPAYCLAVMLDVGTN 255
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+DP+Y GLR+RR
Sbjct: 256 NEELLNDPMYTGLRERR 272
Score = 48.8 bits (111), Expect = 1e-04
Identities = 25/84 (29%), Positives = 41/84 (48%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
Q E+C ++++ N + +YIYL L RN LFY + N +P+
Sbjct: 111 QAEICSNLLESFTNNVQQYIYLTYLSRRNRRLFYYLLLSNPDRFVPMTDASGSIDLLMVH 170
Query: 182 GLVYRRPRGLFITIHDKGHVYDVL 253
+++ +GL+I I D GHV +L
Sbjct: 171 RMIHSMGQGLYICIKDLGHVSQIL 194
Score = 44.4 bits (100), Expect = 0.003
Identities = 22/59 (37%), Positives = 35/59 (59%)
Frame = +2
Query: 506 RLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+L +EF +V+++YG L+ +DF A + LE Y + C + D Q AAVA+AG+
Sbjct: 280 QLFEEFTLSVMQQYGPRALILCKDFEAQKAKKQLELYRERQCIVDVDFQCFAAVALAGV 338
>UniRef50_P36013 Cluster: NAD-dependent malic enzyme, mitochondrial
precursor; n=15; Saccharomycetales|Rep: NAD-dependent
malic enzyme, mitochondrial precursor - Saccharomyces
cerevisiae (Baker's yeast)
Length = 669
Score = 70.5 bits (165), Expect = 3e-11
Identities = 35/75 (46%), Positives = 48/75 (64%)
Frame = +1
Query: 265 ETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQS 444
+ DV IVV+D E ILG+GD G G+ I + KLAL T GGI P + LP+ +DVGT +
Sbjct: 228 DKDVDYIVVSDSEGILGIGDQGIGGVRIAISKLALMTLCGGIHPGRVLPVCLDVGTNNKK 287
Query: 445 MLDDPLYIGLRQRRV 489
+ D LY+G + R+
Sbjct: 288 LARDELYMGNKFSRI 302
Score = 59.3 bits (137), Expect = 9e-08
Identities = 30/57 (52%), Positives = 41/57 (71%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L++F++AV + Y + ++ FEDFG NA RLLEKY + +FNDDIQGT AV +A L
Sbjct: 311 LEKFIKAVKKVY-PSAVLHFEDFGVKNARRLLEKYRYELPSFNDDIQGTGAVVMASL 366
Score = 48.0 bits (109), Expect = 2e-04
Identities = 20/62 (32%), Positives = 38/62 (61%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
+ PL K ++ L +N+ L++ + ++ E++PI+YTPT G A + +R+P G+F+
Sbjct: 151 KTPLAKNDFMTSLRVQNKVLYFALIRRHIKELVPIIYTPTEGDAIAAYSHRFRKPEGVFL 210
Query: 218 TI 223
I
Sbjct: 211 DI 212
>UniRef50_A3YYQ0 Cluster: Malate oxidoreductase; n=1; Synechococcus
sp. WH 5701|Rep: Malate oxidoreductase - Synechococcus
sp. WH 5701
Length = 517
Score = 69.7 bits (163), Expect = 6e-11
Identities = 33/72 (45%), Positives = 48/72 (66%)
Frame = +1
Query: 274 VRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
V I++TD + ILG+GD G G+ I +GKLA+YT G+ P + LP+ +DVGT +L+
Sbjct: 99 VDLILITDSQGILGIGDQGIGGIEICLGKLAVYTLCAGLDPARVLPLVLDVGTDRVELLE 158
Query: 454 DPLYIGLRQRRV 489
+PLY G R R+
Sbjct: 159 NPLYPGWRHPRL 170
Score = 65.7 bits (153), Expect = 1e-09
Identities = 35/85 (41%), Positives = 50/85 (58%), Gaps = 1/85 (1%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QVE + + N L ++ + + L N LF+RF+AD++ +MPIVYTPTVG A Q+F
Sbjct: 7 QVERHWQAFLSLRNDLERFRFAVALRQANLTLFHRFLADHIEAVMPIVYTPTVGAAIQRF 66
Query: 182 GLVYRRPR-GLFITIHDKGHVYDVL 253
L YR P G+F+ D + VL
Sbjct: 67 SLDYRTPSGGVFLAAPDLERIESVL 91
Score = 48.4 bits (110), Expect = 2e-04
Identities = 25/57 (43%), Positives = 36/57 (63%)
Frame = +2
Query: 506 RLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
R L + A+ R++ L+ +EDFG +A +LLE+Y + +FNDDIQGT VA A
Sbjct: 177 RFLARSVEALQRQF-PGALLHWEDFGTGHAHQLLERYRLQLPSFNDDIQGTRGVACA 232
>UniRef50_Q5C370 Cluster: Malic enzyme; n=1; Schistosoma
japonicum|Rep: Malic enzyme - Schistosoma japonicum
(Blood fluke)
Length = 264
Score = 66.1 bits (154), Expect = 7e-10
Identities = 29/57 (50%), Positives = 39/57 (68%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+DE + ++ YG N + F D N+NAF+LLE+Y Y TFNDDIQG+A V V+GL
Sbjct: 10 MDEVVESLSAHYGPNVCLHFADLKNSNAFQLLERYRPNYITFNDDIQGSAVVIVSGL 66
>UniRef50_Q27113 Cluster: Malic enzyme; n=1; Trichomonas
vaginalis|Rep: Malic enzyme - Trichomonas vaginalis
Length = 434
Score = 62.1 bits (144), Expect = 1e-08
Identities = 30/59 (50%), Positives = 39/59 (66%)
Frame = +2
Query: 506 RLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ + EF+ AV +G CLVQFEDF AF+LL+ + + FNDDI+GTAAVA A L
Sbjct: 108 KFVTEFVEAVKEVFGDTCLVQFEDFEMETAFKLLDHFRWRCNCFNDDIEGTAAVAAATL 166
>UniRef50_P40375 Cluster: NAD-dependent malic enzyme; n=3;
Schizosaccharomyces pombe|Rep: NAD-dependent malic
enzyme - Schizosaccharomyces pombe (Fission yeast)
Length = 565
Score = 62.1 bits (144), Expect = 1e-08
Identities = 32/72 (44%), Positives = 45/72 (62%)
Frame = +1
Query: 274 VRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
V I++TD E ILG+GD G G+ I V K L T G+ P++ LPI +DVGT ++
Sbjct: 149 VEYIIITDSEGILGIGDQGVGGVLISVAKGHLMTLCAGLDPNRFLPIVLDVGTNNETHRK 208
Query: 454 DPLYIGLRQRRV 489
+ Y+GLR+ RV
Sbjct: 209 NHQYMGLRKDRV 220
Score = 60.5 bits (140), Expect = 4e-08
Identities = 26/62 (41%), Positives = 40/62 (64%)
Frame = +2
Query: 38 ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
+ PL K +YL L N+ LFY ++ ++ EM+PI+YTPT G A ++F +YR P G ++
Sbjct: 67 DEPLQKNLYLSQLSVTNQTLFYALISQHLIEMIPIIYTPTEGDAIKQFSDIYRYPEGCYL 126
Query: 218 TI 223
I
Sbjct: 127 DI 128
Score = 54.0 bits (124), Expect = 3e-06
Identities = 27/57 (47%), Positives = 35/57 (61%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
LD ++A+ R + FEDFG ANA R+L+ Y FNDDIQGT AVA+A +
Sbjct: 229 LDNVIKAI-REVFPEAFIHFEDFGLANAKRILDHYRPDIACFNDDIQGTGAVALAAI 284
>UniRef50_A7R628 Cluster: Chromosome undetermined scaffold_1141,
whole genome shotgun sequence; n=3; Vitis vinifera|Rep:
Chromosome undetermined scaffold_1141, whole genome
shotgun sequence - Vitis vinifera (Grape)
Length = 236
Score = 54.4 bits (125), Expect = 2e-06
Identities = 27/57 (47%), Positives = 36/57 (63%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L EFM AV + YG+ + +FED+ N NAF LL +Y + N DIQGT V ++GL
Sbjct: 134 LHEFMSAVNQNYGEK-VTRFEDYANHNAFELLARYNTTHLVPNGDIQGTTYVVLSGL 189
>UniRef50_Q9S4T5 Cluster: NAD-malate oxidoreductase homolog; n=15;
Legionellales|Rep: NAD-malate oxidoreductase homolog -
Legionella pneumophila
Length = 117
Score = 53.6 bits (123), Expect = 4e-06
Identities = 23/56 (41%), Positives = 37/56 (66%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLA 169
QV+ L +Y L ++IYL L D+N+ +FY+ ++ ++ EM+PI+YTP VG A
Sbjct: 62 QVKRAYLQYSSYTTRLQQHIYLNNLHDKNQIVFYKLLSRHLGEMLPIIYTPIVGAA 117
>UniRef50_A5B3Q7 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 176
Score = 48.8 bits (111), Expect = 1e-04
Identities = 24/57 (42%), Positives = 34/57 (59%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L +FM V + YG+ + +FED+ N NAF LL +Y + N DIQ T V ++GL
Sbjct: 85 LHKFMSTVKQNYGEK-VTRFEDYANHNAFELLARYNTTHLVPNGDIQXTTYVVLSGL 140
>UniRef50_Q8U225 Cluster: Malate oxidoreductase; n=41; cellular
organisms|Rep: Malate oxidoreductase - Pyrococcus
furiosus
Length = 435
Score = 42.7 bits (96), Expect = 0.008
Identities = 25/55 (45%), Positives = 31/55 (56%), Gaps = 1/55 (1%)
Frame = +1
Query: 286 VVTDGERILGLGDFGA-CGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSM 447
VVTDG ILGLG+ G GM + GK L+ AL GI L T DV I +++
Sbjct: 77 VVTDGSAILGLGNIGVLAGMPVMEGKCVLFKALAGIDAFPILIDTQDVEKIVETI 131
>UniRef50_Q8S484 Cluster: Putative NADP-dependent malic enzyme; n=1;
Zea mays|Rep: Putative NADP-dependent malic enzyme - Zea
mays (Maize)
Length = 309
Score = 41.1 bits (92), Expect = 0.024
Identities = 16/25 (64%), Positives = 19/25 (76%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFG 330
NWP +++ I VTD ERILGLGD G
Sbjct: 257 NWPHRNIQVIFVTDSERILGLGDLG 281
Score = 35.1 bits (77), Expect = 1.6
Identities = 13/24 (54%), Positives = 18/24 (75%)
Frame = +2
Query: 86 NEHLFYRFVADNVAEMMPIVYTPT 157
+E LFY+ + DNV E++P VYT T
Sbjct: 225 DERLFYKLLIDNVVELLPFVYTTT 248
>UniRef50_Q3W7J3 Cluster: Malate dehydrogenase; n=1; Frankia sp.
EAN1pec|Rep: Malate dehydrogenase - Frankia sp. EAN1pec
Length = 464
Score = 40.7 bits (91), Expect = 0.032
Identities = 22/48 (45%), Positives = 29/48 (60%), Gaps = 1/48 (2%)
Frame = +1
Query: 283 IVVTDGERILGLGDFG-ACGMGIPVGKLALYTALGGIKPHQCLPITID 423
IVVTDG +LGLGD G A + + GK AL+ GG+ +PI +D
Sbjct: 142 IVVTDGTAVLGLGDIGPAAALPVMEGKAALFKHFGGV---DAVPICLD 186
>UniRef50_A5C5G3 Cluster: Malic enzyme; n=1; Vitis vinifera|Rep:
Malic enzyme - Vitis vinifera (Grape)
Length = 226
Score = 39.1 bits (87), Expect = 0.098
Identities = 18/41 (43%), Positives = 24/41 (58%)
Frame = +2
Query: 560 LVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
+ +FED N NAF LL +Y + N DIQGT ++GL
Sbjct: 5 VTRFEDSANHNAFELLARYHTTHLVPNGDIQGTTFXVLSGL 45
>UniRef50_Q5Y0M3 Cluster: Malate oxidoreductase; n=8; cellular
organisms|Rep: Malate oxidoreductase - uncultured
archaeon GZfos12E1
Length = 444
Score = 39.1 bits (87), Expect = 0.098
Identities = 20/47 (42%), Positives = 28/47 (59%), Gaps = 1/47 (2%)
Frame = +1
Query: 286 VVTDGERILGLGDFGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 423
+VTDG R+LGLGD GA + + GK L+ LGG+ P+ +D
Sbjct: 84 IVTDGTRVLGLGDVGAEAALPVMEGKAMLFKYLGGV---DAFPLCLD 127
>UniRef50_P54572 Cluster: Probable NAD-dependent malic enzyme 1;
n=4; Bacteria|Rep: Probable NAD-dependent malic enzyme 1
- Bacillus subtilis
Length = 439
Score = 39.1 bits (87), Expect = 0.098
Identities = 22/47 (46%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +1
Query: 286 VVTDGERILGLGDFGA-CGMGIPVGKLALYTALGGIKPHQCLPITID 423
+VTDG ILGLG+ G+ GM + GK AL+ L GI +PI +D
Sbjct: 142 IVTDGTAILGLGNIGSVAGMPVMEGKAALFDQLAGISG---IPILLD 185
>UniRef50_A0DE35 Cluster: Malic enzyme; n=3; Paramecium
tetraurelia|Rep: Malic enzyme - Paramecium tetraurelia
Length = 687
Score = 38.3 bits (85), Expect = 0.17
Identities = 18/38 (47%), Positives = 26/38 (68%), Gaps = 1/38 (2%)
Frame = +1
Query: 280 AIVVTDGERILGLGDFG-ACGMGIPVGKLALYTALGGI 390
A ++T+G ILGLG+ G + G+ + GK L+ ALGGI
Sbjct: 337 AAIITNGTAILGLGNIGPSAGLPVMEGKSVLFNALGGI 374
>UniRef50_Q4SN44 Cluster: Chromosome 8 SCAF14543, whole genome
shotgun sequence; n=1; Tetraodon nigroviridis|Rep:
Chromosome 8 SCAF14543, whole genome shotgun sequence -
Tetraodon nigroviridis (Green puffer)
Length = 212
Score = 37.1 bits (82), Expect = 0.40
Identities = 20/58 (34%), Positives = 28/58 (48%), Gaps = 2/58 (3%)
Frame = +2
Query: 308 FWAWAISARAGWASLWAN--SRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGS 475
+W+ A +A WA W+ SR R + + P+ W RS S W T C+SGS
Sbjct: 147 WWSAAGTAGGAWAGFWSRAWSRKRVRGALTGYTHMSPTPPRWGRSSSGSWAT-CSSGS 203
>UniRef50_Q8R7R6 Cluster: Malic enzyme; n=10; cellular
organisms|Rep: Malic enzyme - Thermoanaerobacter
tengcongensis
Length = 394
Score = 36.7 bits (81), Expect = 0.52
Identities = 21/57 (36%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +1
Query: 286 VVTDGERILGLGDFGA-CGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
VVTDG +LGLG+ G + + GK L+ GGI T DV I +++L+
Sbjct: 66 VVTDGSAVLGLGNIGPYAALPVMEGKAVLFKEFGGIDAIPICLATQDVDEIIRTVLN 122
>UniRef50_Q2KKD0 Cluster: Oxalacetate decarboxylase; n=11;
Lactobacillales|Rep: Oxalacetate decarboxylase -
Enterococcus faecalis (Streptococcus faecalis)
Length = 390
Score = 36.3 bits (80), Expect = 0.69
Identities = 19/62 (30%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +1
Query: 259 WPETDVRAIVVTDGERILGLGDFGA-CGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
W + V+TDG +LGLG+ G G+ I GK LY L G+ +P+ ++ ++
Sbjct: 55 WTISGKLIAVITDGSAVLGLGNMGTQAGLPIVEGKALLYKNLAGV---DAIPLALEQKSV 111
Query: 436 TQ 441
+
Sbjct: 112 DE 113
>UniRef50_Q73UK5 Cluster: Putative uncharacterized protein; n=1;
Mycobacterium avium subsp. paratuberculosis|Rep:
Putative uncharacterized protein - Mycobacterium
paratuberculosis
Length = 291
Score = 35.9 bits (79), Expect = 0.91
Identities = 25/62 (40%), Positives = 30/62 (48%), Gaps = 3/62 (4%)
Frame = +3
Query: 264 GDGRPRHCCNRRRTYFGPGRFRRV---RDGHPCGQTRALHRARRHQASSVSTHHYRRGYD 434
G GRPR R+ PGR +V R G P G A HR RR + HH +RG+
Sbjct: 143 GPGRPR----RQHLRVVPGRLPQVLRRRGGRPAGDLAA-HR-RRQARPVAADHHRQRGHH 196
Query: 435 HP 440
HP
Sbjct: 197 HP 198
>UniRef50_Q9HKY7 Cluster: Malate oxidoreductase (Malic enzyme)
related protein; n=17; Archaea|Rep: Malate
oxidoreductase (Malic enzyme) related protein -
Thermoplasma acidophilum
Length = 467
Score = 35.9 bits (79), Expect = 0.91
Identities = 19/48 (39%), Positives = 28/48 (58%), Gaps = 1/48 (2%)
Frame = +1
Query: 286 VVTDGERILGLGDFG-ACGMGIPVGKLALYTALGGIKPHQCLPITIDV 426
++TDG R+LGLG+ G M + GK ++ LGG+ +PI I V
Sbjct: 94 ILTDGTRVLGLGNIGPEAAMPVMEGKALIFNYLGGV---NAIPIPIRV 138
>UniRef50_A1CKF3 Cluster: Stress response protein (Ish1), putative;
n=5; Pezizomycotina|Rep: Stress response protein (Ish1),
putative - Aspergillus clavatus
Length = 516
Score = 35.5 bits (78), Expect = 1.2
Identities = 30/84 (35%), Positives = 40/84 (47%), Gaps = 9/84 (10%)
Frame = -1
Query: 271 PSPASSKNIVYMSLIVDRDEEAPW-SAVHKPELLTGQANRRSVHNWHHFSHVIRDETVEQ 95
P+PA K++ SL+ +A W S V KP GQA + HNWHH I D +
Sbjct: 50 PTPADRKDLE--SLV-----KANWDSKVQKP---LGQAAEHTTHNWHHAKEWIFDTWSDS 99
Query: 94 ML--------IAVQEPHKIDVLVK 47
L + V +P K DVL+K
Sbjct: 100 QLKAFLDRHGVPVPQPRKRDVLLK 123
>UniRef50_UPI00003ABEA3 Cluster: PREDICTED: hypothetical protein;
n=1; Gallus gallus|Rep: PREDICTED: hypothetical protein
- Gallus gallus
Length = 209
Score = 34.7 bits (76), Expect = 2.1
Identities = 26/73 (35%), Positives = 36/73 (49%), Gaps = 2/73 (2%)
Frame = +2
Query: 320 AISARA-GWASLWANSRSTPRSEASSLISVYPSLSTWVR-SPSRCWTTRCTSGSGSGACW 493
AIS + G +L +S PRS ++ ++ S S WVR P CW CT + S
Sbjct: 66 AISVHSTGEEALGLSSIPVPRSSGKTVKTLARSASPWVRLEPVPCWVKDCTGLACSADHG 125
Query: 494 PRLRRLLDEFMRA 532
PR R LL ++A
Sbjct: 126 PR-RHLLKANLQA 137
>UniRef50_Q1GLN4 Cluster: Putative uncharacterized protein; n=1;
Silicibacter sp. TM1040|Rep: Putative uncharacterized
protein - Silicibacter sp. (strain TM1040)
Length = 547
Score = 34.7 bits (76), Expect = 2.1
Identities = 19/57 (33%), Positives = 31/57 (54%)
Frame = -2
Query: 495 GQHAPLPEPDVQRVVQHRLGDRTHVDSDG*TLMRLDASERGVEREFAHRDAHPARAE 325
G+ A LP+PD R + + + T + T++R A ERG +R+ +A ARA+
Sbjct: 145 GEGAALPDPDQLRALVSTVREETSEARE--TILRAKAQERGFQRQMKDLEAELARAQ 199
>UniRef50_Q8PVS2 Cluster: Conserved protein; n=3;
Methanosarcina|Rep: Conserved protein - Methanosarcina
mazei (Methanosarcina frisia)
Length = 330
Score = 34.7 bits (76), Expect = 2.1
Identities = 17/53 (32%), Positives = 25/53 (47%)
Frame = +2
Query: 491 WPRLRRLLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDI 649
WPR+++ E + + L+ FG RLLE+Y+ Y F DDI
Sbjct: 6 WPRIKKTRTELDDRIFHLFESFLLIIIILFGIQAMIRLLEEYLGIYAEFTDDI 58
>UniRef50_Q580K5 Cluster: Putative uncharacterized protein; n=3;
Trypanosoma|Rep: Putative uncharacterized protein -
Trypanosoma brucei
Length = 564
Score = 34.3 bits (75), Expect = 2.8
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +1
Query: 262 PETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGI 390
P +DV + V D RI+ + ++GACG G K Y +G +
Sbjct: 376 PHSDVVCVNVDDSGRIVSIHNYGACGRGSDTDKPHAYHKIGDV 418
>UniRef50_Q7URP6 Cluster: Putative uncharacterized protein; n=1;
Pirellula sp.|Rep: Putative uncharacterized protein -
Rhodopirellula baltica
Length = 7223
Score = 33.9 bits (74), Expect = 3.7
Identities = 23/73 (31%), Positives = 37/73 (50%), Gaps = 2/73 (2%)
Frame = +1
Query: 262 PETDVRAIVVTDGE--RILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
P TD A D + R++ +G GA G + +L Y A+ G+ P Q L + T+
Sbjct: 4096 PPTDFPADFDEDAQDLRVIKIGLPGAAGASVDA-RLLTYDAVTGLAPVQTLATANGMLTL 4154
Query: 436 TQSMLDDPLYIGL 474
T S++ DP+ G+
Sbjct: 4155 TFSLVADPMNPGM 4167
>UniRef50_Q3I797 Cluster: Putative uncharacterized protein; n=10;
Trichoplax|Rep: Putative uncharacterized protein -
Trichoplax sp. BZ46
Length = 237
Score = 33.5 bits (73), Expect = 4.9
Identities = 27/106 (25%), Positives = 40/106 (37%), Gaps = 3/106 (2%)
Frame = +3
Query: 69 WGSWTAMSICST---VSSRITWLK*CQLCTLRRLAWPVRSSGLCTADQGASSSRSTIRDM 239
WGSW+A + CS V + K C++ RS T G +S ST
Sbjct: 100 WGSWSAATACSNSCGVGNLTEARKRCKVNDANHCEGETRSVACTTTCSGTGASTSTPSTA 159
Query: 240 YTMFLELAGDGRPRHCCNRRRTYFGPGRFRRVRDGHPCGQTRALHR 377
+ + GD C N T R +V D + C + L++
Sbjct: 160 WAQWSAYVGDACSNPCGNGTNTLI--RRRCKVDDTNNCENQQLLNQ 203
>UniRef50_UPI00015555BF Cluster: PREDICTED: similar to
leishmanolysin-like (metallopeptidase M8 family),
partial; n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
similar to leishmanolysin-like (metallopeptidase M8
family), partial - Ornithorhynchus anatinus
Length = 639
Score = 33.1 bits (72), Expect = 6.4
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = +2
Query: 350 LWANSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGACWP 496
LW+++ P ++ ++ +P W + SRCW C + SG P
Sbjct: 171 LWSSTYDLPAPREAAWLAWWPPSGFWWPTSSRCWPASCCACGTSGPVIP 219
>UniRef50_Q47IV3 Cluster: Putative uncharacterized protein; n=1;
Dechloromonas aromatica RCB|Rep: Putative
uncharacterized protein - Dechloromonas aromatica
(strain RCB)
Length = 555
Score = 32.7 bits (71), Expect = 8.5
Identities = 24/87 (27%), Positives = 43/87 (49%)
Frame = +2
Query: 260 GRRRTSAPLL*PTENVFWAWAISARAGWASLWANSRSTPRSEASSLISVYPSLSTWVRSP 439
GRR+ + +E+V + ARAG+ +W +++S + + S+ P L+ +SP
Sbjct: 306 GRRKYDEARIRNSESVL---DVVARAGFRVVWVDNQSGCKGVCKGVESIRPDLA---KSP 359
Query: 440 SRCWTTRCTSGSGSGACWPRLRRLLDE 520
C+ C GA L++L+DE
Sbjct: 360 DHCFGGECQ----DGALVDSLKQLVDE 382
>UniRef50_Q08WN0 Cluster: Putative uncharacterized protein; n=1;
Stigmatella aurantiaca DW4/3-1|Rep: Putative
uncharacterized protein - Stigmatella aurantiaca DW4/3-1
Length = 444
Score = 32.7 bits (71), Expect = 8.5
Identities = 31/100 (31%), Positives = 44/100 (44%), Gaps = 8/100 (8%)
Frame = +3
Query: 156 RLAWPVRSSG---LCTADQGASSSRSTIRDMYTMFLELAGDGRPRHCCNRRRTYFGPG-- 320
RLA P+R SG L G +R +R ++ L R R C+RRR G
Sbjct: 266 RLASPLRGSGGRRLIPPVPGLRIARRGVRGLWVGRRRL----RIRRGCDRRRVRHRRGLR 321
Query: 321 ---RFRRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGY 431
R RR G PC ++R L R + Q++ +RR +
Sbjct: 322 RMLRGRRHHRGRPCNRSRGLGRGQIQQSTHCWLLRHRRRF 361
>UniRef50_A7CYR8 Cluster: D-isomer specific 2-hydroxyacid
dehydrogenase NAD-binding precursor; n=1; Opitutaceae
bacterium TAV2|Rep: D-isomer specific 2-hydroxyacid
dehydrogenase NAD-binding precursor - Opitutaceae
bacterium TAV2
Length = 349
Score = 32.7 bits (71), Expect = 8.5
Identities = 17/63 (26%), Positives = 31/63 (49%), Gaps = 3/63 (4%)
Frame = -1
Query: 313 PKYVLRR---LQQWRGRPSPASSKNIVYMSLIVDRDEEAPWSAVHKPELLTGQANRRSVH 143
PK L R + W G +PA +++ + ++L+ R++ A +H P++ ANR
Sbjct: 105 PKRFLERGGIVSNWGGIAAPAVAEHALLLALMALRNQRAWTQIIHHPDITRPPANRLGTR 164
Query: 142 NWH 134
H
Sbjct: 165 TLH 167
>UniRef50_A0T6Y3 Cluster: Putative uncharacterized protein; n=1;
Burkholderia ambifaria MC40-6|Rep: Putative
uncharacterized protein - Burkholderia ambifaria MC40-6
Length = 748
Score = 32.7 bits (71), Expect = 8.5
Identities = 23/57 (40%), Positives = 26/57 (45%)
Frame = +3
Query: 255 ELAGDGRPRHCCNRRRTYFGPGRFRRVRDGHPCGQTRALHRARRHQASSVSTHHYRR 425
E A R R +RRR G R +RDGH A RARR +A VS RR
Sbjct: 123 EQAARTRRRFADHRRRLLRGRRDLRGIRDGHRRDVHPARDRARRGRADRVSRRVARR 179
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 688,718,713
Number of Sequences: 1657284
Number of extensions: 15282956
Number of successful extensions: 52844
Number of sequences better than 10.0: 88
Number of HSP's better than 10.0 without gapping: 50358
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 52806
length of database: 575,637,011
effective HSP length: 98
effective length of database: 413,223,179
effective search space used: 52892566912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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