Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00175
         (682 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|...    62   7e-11
SPAC10F6.15 |||S. pombe specific UPF0300 family protein 1|Schizo...    27   2.5  
SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa...    26   4.4  
SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|c...    26   5.8  
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    25   7.7  
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c...    25   7.7  

>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 565

 Score = 62.1 bits (144), Expect = 7e-11
 Identities = 32/72 (44%), Positives = 45/72 (62%)
 Frame = +1

Query: 274 VRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTITQSMLD 453
           V  I++TD E ILG+GD G  G+ I V K  L T   G+ P++ LPI +DVGT  ++   
Sbjct: 149 VEYIIITDSEGILGIGDQGVGGVLISVAKGHLMTLCAGLDPNRFLPIVLDVGTNNETHRK 208

Query: 454 DPLYIGLRQRRV 489
           +  Y+GLR+ RV
Sbjct: 209 NHQYMGLRKDRV 220



 Score = 60.5 bits (140), Expect = 2e-10
 Identities = 26/62 (41%), Positives = 40/62 (64%)
 Frame = +2

Query: 38  ENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFI 217
           + PL K +YL  L   N+ LFY  ++ ++ EM+PI+YTPT G A ++F  +YR P G ++
Sbjct: 67  DEPLQKNLYLSQLSVTNQTLFYALISQHLIEMIPIIYTPTEGDAIKQFSDIYRYPEGCYL 126

Query: 218 TI 223
            I
Sbjct: 127 DI 128



 Score = 54.0 bits (124), Expect = 2e-08
 Identities = 27/57 (47%), Positives = 35/57 (61%)
 Frame = +2

Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
           LD  ++A+ R       + FEDFG ANA R+L+ Y      FNDDIQGT AVA+A +
Sbjct: 229 LDNVIKAI-REVFPEAFIHFEDFGLANAKRILDHYRPDIACFNDDIQGTGAVALAAI 284


>SPAC10F6.15 |||S. pombe specific UPF0300 family protein
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 432

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 14/43 (32%), Positives = 18/43 (41%)
 Frame = +3

Query: 315 PGRFRRVRDGHPCGQTRALHRARRHQASSVSTHHYRRGYDHPV 443
           P +   + D H C QT   H  R HQA       Y R  + P+
Sbjct: 128 PDQVYHLIDNHSCIQTAVSHLLRNHQALFKYLCDYLRSGEFPL 170


>SPAC23C4.19 |spt5||transcription elongation factor
           Spt5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 990

 Score = 26.2 bits (55), Expect = 4.4
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = -2

Query: 429 THVDSDG*TLMRLDASERGVEREFAHRD 346
           T V SDG   +RLD   RG+ + F H D
Sbjct: 501 TMVSSDG---LRLDVPSRGLRKRFRHGD 525


>SPAC14C4.12c |||SWIRM domain protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 297

 Score = 25.8 bits (54), Expect = 5.8
 Identities = 12/21 (57%), Positives = 13/21 (61%)
 Frame = -1

Query: 322 RPGPKYVLRRLQQWRGRPSPA 260
           R  PK  LRR +QW  R SPA
Sbjct: 85  RDSPKIWLRRERQWLQRHSPA 105


>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 676

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 11/29 (37%), Positives = 16/29 (55%), Gaps = 2/29 (6%)
 Frame = +3

Query: 45  HLTSTSILWGSWTAMSICS--TVSSRITW 125
           HL  TS++ G+W  +   S   VS  +TW
Sbjct: 71  HLNHTSVMTGNWNILPYPSFGKVSPNVTW 99


>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1111

 Score = 25.4 bits (53), Expect = 7.7
 Identities = 13/45 (28%), Positives = 26/45 (57%), Gaps = 5/45 (11%)
 Frame = +2

Query: 533 VVRRYGQNCLVQ-----FEDFGNANAFRLLEKYMNKYCTFNDDIQ 652
           V+ R G +C+V      F D+G A+   + EK +++  TF+ +++
Sbjct: 538 VISRSGDDCIVALCDQWFLDYGEASWKAVTEKALDRLNTFSPEVR 582


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,734,354
Number of Sequences: 5004
Number of extensions: 57669
Number of successful extensions: 189
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 313902888
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)