Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00175
(682 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79750.1 68414.m09304 malate oxidoreductase, putative similar... 126 1e-29
At2g19900.1 68415.m02326 malate oxidoreductase, putative similar... 124 4e-29
At5g11670.1 68418.m01364 malate oxidoreductase, putative similar... 124 7e-29
At5g25880.1 68418.m03071 malate oxidoreductase, putative similar... 120 7e-28
At2g13560.1 68415.m01495 malate oxidoreductase, putative similar... 101 3e-22
At4g00570.1 68417.m00080 malate oxidoreductase, putative similar... 98 4e-21
At2g35110.1 68415.m04307 HEM protein-related weak similarity to ... 30 1.6
At5g67440.1 68418.m08503 phototropic-responsive NPH3 family prot... 29 2.8
At1g78750.1 68414.m09178 F-box family protein contains F-box dom... 29 2.8
>At1g79750.1 68414.m09304 malate oxidoreductase, putative similar to
malate oxidoreductase (NADP-dependent malic enzyme)
GB:P34105 (Populus balsamifera subsp. trichocarpa)
Length = 646
Score = 126 bits (304), Expect = 1e-29
Identities = 55/77 (71%), Positives = 65/77 (84%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPE +++ IVVTDGERILGLGD G GMGIPVGKL+LYTALGG++P CLP+TIDVGT
Sbjct: 231 NWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTN 290
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+D YIGLRQRR
Sbjct: 291 NEKLLNDEFYIGLRQRR 307
Score = 98.3 bits (234), Expect = 4e-21
Identities = 40/85 (47%), Positives = 64/85 (75%)
Frame = +2
Query: 2 QVELCKLSIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKF 181
QV+ ++ Y+ PL KY+ +M L + NE LFY+ + D+V E++P++YTPTVG ACQK+
Sbjct: 146 QVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKY 205
Query: 182 GLVYRRPRGLFITIHDKGHVYDVLR 256
G ++ RP+GLFI++ +KG +++VLR
Sbjct: 206 GSIFLRPQGLFISLKEKGKIHEVLR 230
Score = 74.5 bits (175), Expect = 6e-14
Identities = 34/58 (58%), Positives = 42/58 (72%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L+ EFM AV + YG+ ++QFEDF N NAF LL KY + FNDDIQGTA+V +AGL
Sbjct: 316 LMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGL 373
>At2g19900.1 68415.m02326 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P51615) {Vitis vinifera}
Length = 581
Score = 124 bits (300), Expect = 4e-29
Identities = 54/77 (70%), Positives = 65/77 (84%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWPE +++ IVVTDGERILGLGD G GMGIPVGKLALY+ALGG++P CLP+TIDVGT
Sbjct: 166 NWPERNIQVIVVTDGERILGLGDLGCQGMGIPVGKLALYSALGGVRPSACLPVTIDVGTN 225
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+D YIGLRQ+R
Sbjct: 226 NEKLLNDEFYIGLRQKR 242
Score = 106 bits (254), Expect = 2e-23
Identities = 47/83 (56%), Positives = 62/83 (74%)
Frame = +2
Query: 23 SIDTYENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRP 202
+I Y+ PL KY+ L L +RNE LFY+ + DNV E++PIVYTPTVG ACQKFG ++RRP
Sbjct: 88 NIRQYQFPLQKYMALTELQERNERLFYKLLIDNVEELLPIVYTPTVGEACQKFGSIFRRP 147
Query: 203 RGLFITIHDKGHVYDVLRTGRRR 271
+GLFI++ DKG + DVL+ R
Sbjct: 148 QGLFISLKDKGKILDVLKNWPER 170
Score = 79.4 bits (187), Expect = 2e-15
Identities = 36/58 (62%), Positives = 44/58 (75%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
LL+EFM AV + YG+ L+QFEDF N NAF LL KY + + FNDDIQGTA+V +AGL
Sbjct: 251 LLNEFMSAVKQNYGEKVLIQFEDFANHNAFELLAKYSDTHLVFNDDIQGTASVVLAGL 308
>At5g11670.1 68418.m01364 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP|P12628) {Phaseolus vulgaris}
Length = 588
Score = 124 bits (298), Expect = 7e-29
Identities = 55/77 (71%), Positives = 64/77 (83%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP+ ++ IVVTDGERILGLGD G GMGIPVGKL+LYTALGGI+P CLPITIDVGT
Sbjct: 173 NWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTN 232
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L+D YIGL+QRR
Sbjct: 233 NEKLLNDEFYIGLKQRR 249
Score = 99 bits (238), Expect = 1e-21
Identities = 40/79 (50%), Positives = 62/79 (78%)
Frame = +2
Query: 35 YENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLF 214
Y PL +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G ++R+P+GL+
Sbjct: 99 YTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIFRKPQGLY 158
Query: 215 ITIHDKGHVYDVLRTGRRR 271
I++++KG + +VL+ +R
Sbjct: 159 ISLNEKGKILEVLKNWPQR 177
Score = 76.6 bits (180), Expect = 1e-14
Identities = 36/57 (63%), Positives = 42/57 (73%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L EFM AV + YG+ LVQFEDF N NAF LL KY + + FNDDIQGTA+V +AGL
Sbjct: 259 LHEFMCAVKQNYGEKVLVQFEDFANHNAFDLLSKYSDSHLVFNDDIQGTASVVLAGL 315
>At5g25880.1 68418.m03071 malate oxidoreductase, putative similar to
NADP-dependent malic enzyme (EC 1.1.1.40) (NADP-ME)
(SP:P12628) {Phaseolus vulgaris}
Length = 588
Score = 120 bits (290), Expect = 7e-28
Identities = 53/77 (68%), Positives = 64/77 (83%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP+ ++ IVVTDGERILGLGD G GMGIPVGKL+LYTALGGI+P CLPITIDVGT
Sbjct: 173 NWPQRGIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGIRPSACLPITIDVGTN 232
Query: 436 TQSMLDDPLYIGLRQRR 486
+ +L++ YIGL+Q+R
Sbjct: 233 NEKLLNNEFYIGLKQKR 249
Score = 101 bits (243), Expect = 3e-22
Identities = 42/79 (53%), Positives = 61/79 (77%)
Frame = +2
Query: 35 YENPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLF 214
Y PL +Y+ LM L +RNE LFY+ + DNV E++P+VYTPTVG ACQK+G +YRRP+GL+
Sbjct: 99 YTVPLQRYMALMDLQERNERLFYKLLIDNVEELLPVVYTPTVGEACQKYGSIYRRPQGLY 158
Query: 215 ITIHDKGHVYDVLRTGRRR 271
I++ +KG + +VL+ +R
Sbjct: 159 ISLKEKGKILEVLKNWPQR 177
Score = 75.8 bits (178), Expect = 3e-14
Identities = 35/57 (61%), Positives = 42/57 (73%)
Frame = +2
Query: 512 LDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
L EFM AV + YG+ LVQFEDF N +AF LL KY + + FNDDIQGTA+V +AGL
Sbjct: 259 LQEFMCAVKQNYGEKVLVQFEDFANHHAFELLSKYCSSHLVFNDDIQGTASVVLAGL 315
>At2g13560.1 68415.m01495 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 62 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37221) {Solanum
tuberosum}
Length = 623
Score = 101 bits (243), Expect = 3e-22
Identities = 46/78 (58%), Positives = 56/78 (71%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP V IVVTDG RILGLGD G G+GI VGKL LY A GI P + LP+ IDVGT
Sbjct: 180 NWPAEQVDMIVVTDGSRILGLGDLGVHGIGIAVGKLDLYVAAAGINPQRVLPVMIDVGTN 239
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ + +DP+Y+GL+QRR+
Sbjct: 240 NEKLRNDPMYLGLQQRRL 257
Score = 74.5 bits (175), Expect = 6e-14
Identities = 31/71 (43%), Positives = 48/71 (67%)
Frame = +2
Query: 41 NPLNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFIT 220
N L K+ L L DRNE ++Y+ + +N+ E PIVYTPTVGL CQ + ++RRPRG++ +
Sbjct: 108 NALAKWRILNRLHDRNETMYYKVLINNIEEYAPIVYTPTVGLVCQNYSGLFRRPRGMYFS 167
Query: 221 IHDKGHVYDVL 253
D+G + ++
Sbjct: 168 AEDRGEMMSMV 178
Score = 66.5 bits (155), Expect = 2e-11
Identities = 32/58 (55%), Positives = 43/58 (74%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++DEFM AV R+ + +VQFEDF + AF+LL++Y Y FNDD+QGTA VA+AGL
Sbjct: 265 VIDEFMEAVYTRW-PHVIVQFEDFQSKWAFKLLQRYRCTYRMFNDDVQGTAGVAIAGL 321
>At4g00570.1 68417.m00080 malate oxidoreductase, putative similar to
NAD-dependent malic enzyme 59 kDa isoform, mitochondrial
precursor (EC 1.1.1.39) (NAD-ME) (SP:P37225) {Solanum
tuberosum}
Length = 607
Score = 98.3 bits (234), Expect = 4e-21
Identities = 43/78 (55%), Positives = 55/78 (70%)
Frame = +1
Query: 256 NWPETDVRAIVVTDGERILGLGDFGACGMGIPVGKLALYTALGGIKPHQCLPITIDVGTI 435
NWP V IV+TDG RILGLGD G G+GIP+GKL +Y A GI P + LPI +DVGT
Sbjct: 173 NWPAPQVDMIVITDGSRILGLGDLGVQGIGIPIGKLDMYVAAAGINPQRVLPIMLDVGTN 232
Query: 436 TQSMLDDPLYIGLRQRRV 489
+ +L + LY+G+RQ R+
Sbjct: 233 NEKLLQNDLYLGVRQPRL 250
Score = 78.2 bits (184), Expect = 5e-15
Identities = 33/69 (47%), Positives = 47/69 (68%)
Frame = +2
Query: 47 LNKYIYLMGLLDRNEHLFYRFVADNVAEMMPIVYTPTVGLACQKFGLVYRRPRGLFITIH 226
L K+ L L DRNE L+YR + DN+ + PI+YTPTVGL CQ + +YRRPRG++ +
Sbjct: 103 LAKWRMLNRLHDRNETLYYRVLIDNIKDFAPIIYTPTVGLVCQNYSGLYRRPRGMYFSAK 162
Query: 227 DKGHVYDVL 253
DKG + ++
Sbjct: 163 DKGEMMSMI 171
Score = 67.3 bits (157), Expect = 9e-12
Identities = 32/58 (55%), Positives = 41/58 (70%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVAGL 682
++DEFM A R+ + +VQFEDF AF LE+Y K+C FNDD+QGTA VA+AGL
Sbjct: 258 IIDEFMEAAFTRWPK-AVVQFEDFQAKWAFGTLERYRKKFCMFNDDVQGTAGVALAGL 314
>At2g35110.1 68415.m04307 HEM protein-related weak similarity to
Membrane-associated protein Hem (Dhem-2)
(Swiss-Prot:P55162) [Drosophila melanogaster]; weak
similarity to Nck-associated protein 1 (NAP 1) (p125Nap1)
(Membrane-associated protein HEM-2) (Swiss-Prot:P55161)
[Rattus norvegicus]
Length = 1339
Score = 29.9 bits (64), Expect = 1.6
Identities = 21/52 (40%), Positives = 29/52 (55%)
Frame = +2
Query: 332 RAGWASLWANSRSTPRSEASSLISVYPSLSTWVRSPSRCWTTRCTSGSGSGA 487
R+ + ++N+ STP S AS S PS+S SPS +T GSGSG+
Sbjct: 1200 RSIYTQYYSNTPSTPLSTASPYHS--PSVSLIHASPSMKNSTTPQRGSGSGS 1249
Score = 27.9 bits (59), Expect = 6.6
Identities = 21/84 (25%), Positives = 27/84 (32%), Gaps = 2/84 (2%)
Frame = +3
Query: 45 HLTSTSILWGSWTAMSICSTVSSRITWLK*CQLCTLRRLAWPVRSSGLCTADQGASSSRS 224
HL S G + C R WL + T R + +R T G S
Sbjct: 535 HLESIPKAQGENVSAITCDLSDFRKDWLSILMIVTSSRSSINIRHLEKATVSTGKEGLLS 594
Query: 225 TIRDMYTMFLE--LAGDGRPRHCC 290
Y +GRP+HCC
Sbjct: 595 EGNAAYNWSRNTMFGPEGRPQHCC 618
>At5g67440.1 68418.m08503 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 579
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/56 (23%), Positives = 26/56 (46%)
Frame = +2
Query: 509 LLDEFMRAVVRRYGQNCLVQFEDFGNANAFRLLEKYMNKYCTFNDDIQGTAAVAVA 676
L+DEF+ +R +C D AN +L++ Y+ + ++ T + +A
Sbjct: 347 LIDEFVTQTEKRDELDCSDDINDSSKANVAKLIDGYLAEISRIETNLSTTKFITIA 402
>At1g78750.1 68414.m09178 F-box family protein contains F-box domain
Pfam:PF00646
Length = 458
Score = 29.1 bits (62), Expect = 2.8
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -1
Query: 610 LEEPKSVSVPEVLELHETVLPVAAHDGAHELVEQ 509
L PK VS+P + ELH +++ A H L+ Q
Sbjct: 165 LASPKFVSLPSLKELHLSIVKFADHMALETLISQ 198
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,705,227
Number of Sequences: 28952
Number of extensions: 326070
Number of successful extensions: 1021
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1020
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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