Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00165
(705 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 56 7e-09
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 47 2e-06
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 46 5e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 36 0.004
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 33 0.030
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 33 0.040
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 32 0.092
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 31 0.12
SPCC1259.02c |||aminopeptidase |Schizosaccharomyces pombe|chr 3|... 29 0.49
SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr 2||... 27 3.5
SPCC1442.02 ||SPCC1450.18|DUF1760 family protein|Schizosaccharom... 26 4.6
SPCC757.07c |ctt1|cta1|catalase|Schizosaccharomyces pombe|chr 3|... 26 6.0
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 26 6.0
SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr 1|... 25 8.0
SPAC16.03c |ura2||dihydroorotase Ura2 |Schizosaccharomyces pombe... 25 8.0
SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca... 25 8.0
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 55.6 bits (128), Expect = 7e-09
Identities = 22/31 (70%), Positives = 23/31 (74%)
Frame = +3
Query: 423 VFDTTKKVLVEFYAPWCGHCKQLVPIYDNSA 515
V D TK VLVEFYAPWCGHCK L P Y+ A
Sbjct: 369 VMDETKDVLVEFYAPWCGHCKNLAPTYEKLA 399
Score = 54.4 bits (125), Expect = 2e-08
Identities = 25/67 (37%), Positives = 43/67 (64%), Gaps = 2/67 (2%)
Frame = +2
Query: 494 PYLRQLGEHFENDDDVIIAKIDATANELEHTKITSFPTIKLYSKDNQVHD--YNGERTLA 667
P +L E + +D +V++AKIDAT N++ I+ FPTI + +++V+ Y G+RTL
Sbjct: 393 PTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMFFKANDKVNPVRYEGDRTLE 451
Query: 668 GLTKFVE 688
L+ F++
Sbjct: 452 DLSAFID 458
Score = 47.6 bits (108), Expect = 2e-06
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +3
Query: 438 KKVLVEFYAPWCGHCKQLVPIYDNSASTL 524
K ++V+FYAPWCGHCK L P Y+++A L
Sbjct: 40 KVLMVKFYAPWCGHCKALAPEYESAADEL 68
Score = 28.3 bits (60), Expect = 1.1
Identities = 12/53 (22%), Positives = 25/53 (47%), Gaps = 2/53 (3%)
Frame = +2
Query: 533 DDVIIAKIDAT--ANELEHTKITSFPTIKLYSKDNQVHDYNGERTLAGLTKFV 685
D + + ++D T + I +PT+ ++ Q+ Y+G R L K++
Sbjct: 71 DGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYM 123
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 47.2 bits (107), Expect = 2e-06
Identities = 18/28 (64%), Positives = 21/28 (75%)
Frame = +3
Query: 423 VFDTTKKVLVEFYAPWCGHCKQLVPIYD 506
V D K VLVEFYA WCG+CK+L P Y+
Sbjct: 154 VMDDKKDVLVEFYADWCGYCKRLAPTYE 181
Score = 45.2 bits (102), Expect = 9e-06
Identities = 15/23 (65%), Positives = 18/23 (78%)
Frame = +3
Query: 438 KKVLVEFYAPWCGHCKQLVPIYD 506
K L+EFYA WCGHCK L P+Y+
Sbjct: 40 KGALIEFYATWCGHCKSLAPVYE 62
Score = 41.5 bits (93), Expect = 1e-04
Identities = 24/71 (33%), Positives = 39/71 (54%), Gaps = 3/71 (4%)
Frame = +2
Query: 482 QTAGPYLRQLGEHFENDDDVIIAKIDATANE--LEHTKITSFPTIKLYSKD-NQVHDYNG 652
++ P +LG FE+ +DV+I KIDA + + IT FPT+ + D ++ Y+
Sbjct: 55 KSLAPVYEELGALFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSN 114
Query: 653 ERTLAGLTKFV 685
R + LT+FV
Sbjct: 115 ARDVDSLTQFV 125
Score = 40.3 bits (90), Expect = 3e-04
Identities = 24/68 (35%), Positives = 40/68 (58%), Gaps = 4/68 (5%)
Frame = +2
Query: 494 PYLRQLGEHFENDDDVIIAKIDAT--ANELEHTKITSFPTIKLYSKDNQVHD--YNGERT 661
P LG+ F+N+ +V I KI+A A+ ++ SFPTIK + KD++ Y G+R+
Sbjct: 178 PTYETLGKVFKNEPNVEIVKINADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRS 237
Query: 662 LAGLTKFV 685
L L +++
Sbjct: 238 LESLIEYI 245
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 46.0 bits (104), Expect = 5e-06
Identities = 18/26 (69%), Positives = 20/26 (76%)
Frame = +3
Query: 447 LVEFYAPWCGHCKQLVPIYDNSASTL 524
LV FYAPWCG+CK+LVP Y AS L
Sbjct: 52 LVVFYAPWCGYCKKLVPTYQKLASNL 77
Score = 29.9 bits (64), Expect = 0.37
Identities = 23/71 (32%), Positives = 33/71 (46%), Gaps = 7/71 (9%)
Frame = +2
Query: 494 PYLRQLGEHFENDDDVIIAKIDATANEL--EHTKITSFPTIKLY---SKDNQVH--DYNG 652
P ++L + + V DA N ++ FPTIKL SK + + DYNG
Sbjct: 68 PTYQKLASNLHSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNG 127
Query: 653 ERTLAGLTKFV 685
+R+ L KFV
Sbjct: 128 DRSYKSLQKFV 138
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 36.3 bits (80), Expect = 0.004
Identities = 13/34 (38%), Positives = 22/34 (64%)
Frame = +3
Query: 420 SVFDTTKKVLVEFYAPWCGHCKQLVPIYDNSAST 521
S+ K V+V+F+A WCG CK + P ++ ++T
Sbjct: 13 SIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNT 46
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 33.5 bits (73), Expect = 0.030
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3
Query: 438 KKVLVEFYAPWCGHCKQLVPIYD 506
K +V+FYA WCG CK L P +
Sbjct: 36 KVTVVDFYADWCGPCKYLKPFLE 58
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 33.1 bits (72), Expect = 0.040
Identities = 11/24 (45%), Positives = 16/24 (66%)
Frame = +3
Query: 444 VLVEFYAPWCGHCKQLVPIYDNSA 515
+L+ FYAPW CKQ+ ++D A
Sbjct: 23 ILLNFYAPWAAPCKQMNQVFDQFA 46
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 31.9 bits (69), Expect = 0.092
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = +3
Query: 450 VEFYAPWCGHCKQLVPIYDNSAS 518
V+ YA WCG CK + P++ AS
Sbjct: 24 VDCYADWCGPCKAISPLFSQLAS 46
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 31.5 bits (68), Expect = 0.12
Identities = 10/20 (50%), Positives = 16/20 (80%)
Frame = +3
Query: 450 VEFYAPWCGHCKQLVPIYDN 509
+++Y P CG CK+L P++DN
Sbjct: 47 IKYYLPSCGACKRLGPMWDN 66
>SPCC1259.02c |||aminopeptidase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 822
Score = 29.5 bits (63), Expect = 0.49
Identities = 16/31 (51%), Positives = 19/31 (61%), Gaps = 1/31 (3%)
Frame = -3
Query: 193 LQDPLVFIFVGIDGDGHDP-VAVGLRDRLQI 104
L+ P+ F FVG+DG GH P A RDR I
Sbjct: 762 LEAPIYFDFVGVDGVGHYPSKASEGRDRASI 792
>SPBC609.01 |||ribonuclease II |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1157
Score = 26.6 bits (56), Expect = 3.5
Identities = 22/62 (35%), Positives = 28/62 (45%)
Frame = -1
Query: 234 RRADGTSSFFIPKNSKILWCSSSSASMATVMILSR*VFATGFKSSRYFSKSPFFLDKKIR 55
RR TSS K KI+W S + + I S V T F ++ F K FL I+
Sbjct: 484 RRNSSTSSGETGKGPKIVWFKPSDKRIPLIAISSDQVPPTFFTNNDDF-KDKVFL-AGIK 541
Query: 54 RW 49
RW
Sbjct: 542 RW 543
>SPCC1442.02 ||SPCC1450.18|DUF1760 family
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 562
Score = 26.2 bits (55), Expect = 4.6
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = +2
Query: 599 FPTIKLYSKDNQVHDYNGERTLAGLTKFVE 688
FPT+ +Q+++Y GE GL ++E
Sbjct: 526 FPTVYFTKISDQINNYEGELPTDGLKYYIE 555
>SPCC757.07c |ctt1|cta1|catalase|Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 6.0
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +3
Query: 657 GHWPASPSSLRPTVK 701
G+WP PSS+RP K
Sbjct: 384 GNWPNYPSSIRPLAK 398
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 25.8 bits (54), Expect = 6.0
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +1
Query: 22 QDLWWKDQIPPSYLLIQEKW 81
+ +WW QIP +L ++KW
Sbjct: 441 RQIWWGHQIPVWKILEEDKW 460
>SPAC13G7.12c |||choline kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 25.4 bits (53), Expect = 8.0
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +2
Query: 77 NGDFEKYLEDLKPVAKTYRDRIMTVAI 157
NG FE+Y+E KT RD +++ +
Sbjct: 130 NGRFEQYMESTTLTCKTIRDPKLSIYV 156
>SPAC16.03c |ura2||dihydroorotase Ura2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 337
Score = 25.4 bits (53), Expect = 8.0
Identities = 24/66 (36%), Positives = 33/66 (50%)
Frame = +2
Query: 35 GKIKYHLLIFLSKKNGDFEKYLEDLKPVAKTYRDRIMTVAIDADEDEHQRILEFFGMKKD 214
G I H L +L++K+ + Y KPVAKT RDR I+A ++ + FFG D
Sbjct: 189 GTITAHHL-YLTQKDWQDDPYCF-CKPVAKTERDR--RALIEAATSKNPKF--FFG--SD 240
Query: 215 EVPSAR 232
P R
Sbjct: 241 SAPHPR 246
>SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non
catalytic subunit Arm1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 811
Score = 25.4 bits (53), Expect = 8.0
Identities = 14/52 (26%), Positives = 26/52 (50%)
Frame = +2
Query: 50 HLLIFLSKKNGDFEKYLEDLKPVAKTYRDRIMTVAIDADEDEHQRILEFFGM 205
H L+ S NGDFEK + K + + ++ + ++E H ++L G+
Sbjct: 611 HSLVQDSIVNGDFEKAVLSAKEMEVLCENNDLSKQLTSEEIVHMKLLIQLGL 662
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,677,853
Number of Sequences: 5004
Number of extensions: 53031
Number of successful extensions: 211
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 192
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 210
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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