BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00162 (609 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|... 124 1e-29 SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12... 124 1e-29 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 31 0.13 SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces... 27 1.6 SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 27 2.1 SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosacchar... 26 5.0 SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 26 5.0 SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynei... 25 6.5 SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharom... 25 8.7 >SPCC31H12.04c |rpl1202|rpl12-2|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 124 bits (299), Expect = 1e-29 Identities = 57/90 (63%), Positives = 78/90 (86%) Frame = +1 Query: 238 EGSQDHVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVIGIA 417 +G + V+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL+++I +A Sbjct: 54 KGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEIIEVA 113 Query: 418 KIMRNRSMARYLSGSVKEILGTAQSVGCTV 507 + MR +S+A+ LSG+VKEILGTA SVGCTV Sbjct: 114 RTMRFKSLAKELSGTVKEILGTAFSVGCTV 143 Score = 102 bits (245), Expect = 4e-23 Identities = 44/58 (75%), Positives = 51/58 (87%) Frame = +2 Query: 80 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKI 253 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++ Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRV 58 >SPCC16C4.13c |rpl1201|rpl12-1, rpl12.1|60S ribosomal protein L12.1/L12A|Schizosaccharomyces pombe|chr 3|||Manual Length = 165 Score = 124 bits (299), Expect = 1e-29 Identities = 57/90 (63%), Positives = 78/90 (86%) Frame = +1 Query: 238 EGSQDHVQLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVIGIA 417 +G + V+LT+QNRQA ++VVPSA+AL+I+ALKEP RDRKK KN+ H+GN+SL+++I +A Sbjct: 54 KGLRVTVKLTIQNRQAAVSVVPSASALVIKALKEPARDRKKDKNVAHSGNVSLDEIIEVA 113 Query: 418 KIMRNRSMARYLSGSVKEILGTAQSVGCTV 507 + MR +S+A+ LSG+VKEILGTA SVGCTV Sbjct: 114 RTMRFKSLAKELSGTVKEILGTAFSVGCTV 143 Score = 102 bits (245), Expect = 4e-23 Identities = 44/58 (75%), Positives = 51/58 (87%) Frame = +2 Query: 80 MPPKFDPNEIKIVNLRCVGGEVGATSSLAPKIGPLGLSPKKVGDDIAKATSDWKGLKI 253 MPPKFDPNE+K + +R VGGEV S+LAPKIGPLGLSPKKVG+DIAKAT DWKGL++ Sbjct: 1 MPPKFDPNEVKTIFMRAVGGEVAGGSTLAPKIGPLGLSPKKVGEDIAKATKDWKGLRV 58 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 31.1 bits (67), Expect = 0.13 Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Frame = -2 Query: 509 PTVHPTDCAVPRISFTEPERYRAIDLFLMIFAIPI-TSSREMLPL 378 P++ P V R+ ++PE DLFL I A+ + TSS+ +LPL Sbjct: 1466 PSLPPLIDLVKRLITSDPEGVLKKDLFLFIRALALRTSSKHLLPL 1510 >SPAPB21F2.03 |||ribosome biogenesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 172 Score = 27.5 bits (58), Expect = 1.6 Identities = 17/72 (23%), Positives = 30/72 (41%) Frame = +1 Query: 259 QLTVQNRQAQIAVVPSAAALIIRALKEPPRDRKKQKNIKHNGNISLEDVIGIAKIMRNRS 438 Q ++ N + V ++ L LKE +D++K ++ S + A+ RNR Sbjct: 27 QASIPNVELSSTVTSNSQVLNNDPLKETKKDKRKDRHFNWQQKFSNPKISASARKRRNRK 86 Query: 439 MARYLSGSVKEI 474 L +V I Sbjct: 87 ARENLKVNVSSI 98 >SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 874 Score = 27.1 bits (57), Expect = 2.1 Identities = 13/39 (33%), Positives = 20/39 (51%) Frame = -3 Query: 553 TPLLMSSIRSCGGLPPQYIQLTVLCQESLLLSQKGTGPL 437 TP+ M +CG +P +L V + + +KGT PL Sbjct: 440 TPIPMVHCETCGAVPVPESELPVKLPDLDKIYEKGTSPL 478 >SPAC6F12.05c |tnr3||thiamine diphosphokinase Tnr3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 569 Score = 25.8 bits (54), Expect = 5.0 Identities = 8/25 (32%), Positives = 15/25 (60%) Frame = -3 Query: 277 CFELSAAHDLETLPVTCGLGNVITH 203 C ++ H ++T+ V CG+G + H Sbjct: 437 CHKIIKEHGIDTIFVLCGMGGRVDH 461 >SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual Length = 380 Score = 25.8 bits (54), Expect = 5.0 Identities = 16/44 (36%), Positives = 19/44 (43%) Frame = +3 Query: 153 HHLWPLKSVLLVCLLKRWVMTLPRPQVTGRVSRSCAADSSKQTS 284 H W V+ V + + T P VTGRV R CA K S Sbjct: 288 HKGWGQSIVIGVAAAGQEISTRPFQLVTGRVWRGCAFGGVKGRS 331 >SPBC216.02 |mcp5|num1, mug21|cortical anchoring factor for dynein Mcp5/Num1|Schizosaccharomyces pombe|chr 2|||Manual Length = 968 Score = 25.4 bits (53), Expect = 6.5 Identities = 12/37 (32%), Positives = 18/37 (48%) Frame = -1 Query: 360 LFTITRRLLKGSDDKGCCRGNNSYLGLSVLNCQLHMI 250 +FT+ R LK + CR N + GLS C + + Sbjct: 805 VFTLPRNNLKSKTNTKKCRDNLNLSGLSSSTCNANSV 841 >SPBC725.14 |arg6||acetylglutamate synthase Arg6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 500 Score = 25.0 bits (52), Expect = 8.7 Identities = 10/18 (55%), Positives = 13/18 (72%) Frame = -1 Query: 159 DDVAPTSPPTHRKFTILI 106 D++A T PP HRK IL+ Sbjct: 249 DELAKTLPPYHRKNLILV 266 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,573,143 Number of Sequences: 5004 Number of extensions: 53490 Number of successful extensions: 126 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 126 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 268287866 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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