BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00159 (496 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si... 105 2e-23 At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id... 102 2e-22 At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si... 44 6e-05 At2g16100.1 68415.m01846 hypothetical protein 29 2.3 At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d... 28 4.0 At4g36080.1 68417.m05136 FAT domain-containing protein / phospha... 27 6.9 >At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637 GI:1710530 from [Arabidopsis thaliana] Length = 146 Score = 105 bits (251), Expect = 2e-23 Identities = 47/80 (58%), Positives = 53/80 (66%) Frame = +2 Query: 14 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 193 MAT+ KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR Sbjct: 1 MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60 Query: 194 FHFRKNKNFCPVLNLDKLWT 253 FH +NK FCP++NLDKLW+ Sbjct: 61 FHKLRNKFFCPIVNLDKLWS 80 >At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) identical to 60S ribosomal protein L27A GB:P49637 [Arabidopsis thaliana] Length = 146 Score = 102 bits (244), Expect = 2e-22 Identities = 46/80 (57%), Positives = 51/80 (63%) Frame = +2 Query: 14 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 193 M T KK RK RGHVS NAGG HHHRI DKYHPGYFGK+GMR Sbjct: 1 MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60 Query: 194 FHFRKNKNFCPVLNLDKLWT 253 FH +NK FCP++NLDKLW+ Sbjct: 61 FHKLRNKFFCPIVNLDKLWS 80 >At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) similar to GB:BAA96068 from [Panax ginseng] Length = 104 Score = 44.0 bits (99), Expect = 6e-05 Identities = 31/82 (37%), Positives = 39/82 (47%), Gaps = 2/82 (2%) Frame = +2 Query: 14 MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 187 M TS+KKTR LR HVS H R + PG G +GM Sbjct: 1 MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39 Query: 188 RNFHFRKNKNFCPVLNLDKLWT 253 R FH +NK +C ++NLDKLW+ Sbjct: 40 RYFHKLRNKFYCQIVNLDKLWS 61 >At2g16100.1 68415.m01846 hypothetical protein Length = 250 Score = 28.7 bits (61), Expect = 2.3 Identities = 9/19 (47%), Positives = 13/19 (68%) Frame = +1 Query: 127 APSQNQHGQVPSWILWQTW 183 +P +Q Q+P WILW+ W Sbjct: 20 SPRTSQFHQLPLWILWRIW 38 >At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-dependent DNA polymerase, putative similar to reverse transcriptase [Arabidopsis thaliana] GI:976278; contains Pfam profile PF00075: RNase H Length = 575 Score = 27.9 bits (59), Expect = 4.0 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%) Frame = +1 Query: 130 PSQNQHGQVPSWILWQTWYEKFPL-*KEQEFLSSFELR*ALDIVSEQTRLKYASAPDGKV 306 P + Q+ W+LW+ W + L + +EF + LR A D + E+ R++ + G Sbjct: 351 PQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDL-EEWRIRTEAESCGTK 409 Query: 307 PVIN 318 P +N Sbjct: 410 PQVN 413 >At4g36080.1 68417.m05136 FAT domain-containing protein / phosphatidylinositol 3- and 4-kinase family protein contains Pfam profiles PF00454: Phosphatidylinositol 3- and 4-kinase, PF02259: FAT domain, PF02260: FATC domain Length = 3839 Score = 27.1 bits (57), Expect = 6.9 Identities = 12/43 (27%), Positives = 23/43 (53%) Frame = +3 Query: 240 ISFGHSL*TDEAEVCICSRWQGPRHQYCQSWILQVARQRQTPQ 368 + FG S T+ E+ + S W+ P Y + ++ A+ +TP+ Sbjct: 2786 VDFGKS--TENYEILLDSLWKAPDWTYLKDHVIPKAQVEETPK 2826 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,828,762 Number of Sequences: 28952 Number of extensions: 194061 Number of successful extensions: 475 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 456 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 474 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 868578304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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