SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00159
         (496 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB) si...   105   2e-23
At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC) id...   102   2e-22
At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA) si...    44   6e-05
At2g16100.1 68415.m01846 hypothetical protein                          29   2.3  
At4g29090.1 68417.m04163 reverse transcriptase, putative / RNA-d...    28   4.0  
At4g36080.1 68417.m05136 FAT domain-containing protein / phospha...    27   6.9  

>At1g23290.1 68414.m02913 60S ribosomal protein L27A (RPL27aB)
           similar to 60S RIBOSOMAL PROTEIN L27A GB:P49637
           GI:1710530 from [Arabidopsis thaliana]
          Length = 146

 Score =  105 bits (251), Expect = 2e-23
 Identities = 47/80 (58%), Positives = 53/80 (66%)
 Frame = +2

Query: 14  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 193
           MAT+ KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MATALKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 194 FHFRKNKNFCPVLNLDKLWT 253
           FH  +NK FCP++NLDKLW+
Sbjct: 61  FHKLRNKFFCPIVNLDKLWS 80


>At1g70600.1 68414.m08133 60S ribosomal protein L27A (RPL27aC)
           identical to 60S ribosomal protein L27A GB:P49637
           [Arabidopsis thaliana]
          Length = 146

 Score =  102 bits (244), Expect = 2e-22
 Identities = 46/80 (57%), Positives = 51/80 (63%)
 Frame = +2

Query: 14  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGKLGMRN 193
           M T  KK RK RGHVS                 NAGG HHHRI  DKYHPGYFGK+GMR 
Sbjct: 1   MTTRFKKNRKKRGHVSAGHGRIGKHRKHPGGRGNAGGMHHHRILFDKYHPGYFGKVGMRY 60

Query: 194 FHFRKNKNFCPVLNLDKLWT 253
           FH  +NK FCP++NLDKLW+
Sbjct: 61  FHKLRNKFFCPIVNLDKLWS 80


>At1g12960.1 68414.m01505 60S ribosomal protein L27A (RPL27aA)
           similar to GB:BAA96068 from [Panax ginseng]
          Length = 104

 Score = 44.0 bits (99), Expect = 6e-05
 Identities = 31/82 (37%), Positives = 39/82 (47%), Gaps = 2/82 (2%)
 Frame = +2

Query: 14  MATSKKKTRKLRGHVSXXXXXXXXXXXXXXXXXNAGGEHHHRINMDKYHPGYFGK--LGM 187
           M TS+KKTR LR HVS                       H R    +  PG  G   +GM
Sbjct: 1   MTTSRKKTRNLREHVSVG---------------------HGRFGKHRKLPGSRGNAGVGM 39

Query: 188 RNFHFRKNKNFCPVLNLDKLWT 253
           R FH  +NK +C ++NLDKLW+
Sbjct: 40  RYFHKLRNKFYCQIVNLDKLWS 61


>At2g16100.1 68415.m01846 hypothetical protein 
          Length = 250

 Score = 28.7 bits (61), Expect = 2.3
 Identities = 9/19 (47%), Positives = 13/19 (68%)
 Frame = +1

Query: 127 APSQNQHGQVPSWILWQTW 183
           +P  +Q  Q+P WILW+ W
Sbjct: 20  SPRTSQFHQLPLWILWRIW 38


>At4g29090.1 68417.m04163 reverse transcriptase, putative /
           RNA-dependent DNA polymerase, putative similar to
           reverse transcriptase [Arabidopsis thaliana] GI:976278;
           contains Pfam profile PF00075: RNase H
          Length = 575

 Score = 27.9 bits (59), Expect = 4.0
 Identities = 18/64 (28%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
 Frame = +1

Query: 130 PSQNQHGQVPSWILWQTWYEKFPL-*KEQEFLSSFELR*ALDIVSEQTRLKYASAPDGKV 306
           P   +  Q+  W+LW+ W  +  L  + +EF +   LR A D + E+ R++  +   G  
Sbjct: 351 PQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDL-EEWRIRTEAESCGTK 409

Query: 307 PVIN 318
           P +N
Sbjct: 410 PQVN 413


>At4g36080.1 68417.m05136 FAT domain-containing protein /
            phosphatidylinositol 3- and 4-kinase family protein
            contains Pfam profiles PF00454: Phosphatidylinositol 3-
            and 4-kinase, PF02259: FAT domain, PF02260: FATC domain
          Length = 3839

 Score = 27.1 bits (57), Expect = 6.9
 Identities = 12/43 (27%), Positives = 23/43 (53%)
 Frame = +3

Query: 240  ISFGHSL*TDEAEVCICSRWQGPRHQYCQSWILQVARQRQTPQ 368
            + FG S  T+  E+ + S W+ P   Y +  ++  A+  +TP+
Sbjct: 2786 VDFGKS--TENYEILLDSLWKAPDWTYLKDHVIPKAQVEETPK 2826


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,828,762
Number of Sequences: 28952
Number of extensions: 194061
Number of successful extensions: 475
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 456
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 474
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 868578304
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -