Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00154
(772 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) 148 5e-36
At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) riboso... 145 3e-35
At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A) 145 3e-35
At3g06190.2 68416.m00712 speckle-type POZ protein-related simila... 33 0.21
At3g06190.1 68416.m00711 speckle-type POZ protein-related simila... 33 0.21
At5g66850.1 68418.m08428 protein kinase family protein contains ... 30 1.5
At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR... 29 3.4
At5g19000.1 68418.m02257 speckle-type POZ protein-related contai... 29 4.5
At4g36190.1 68417.m05149 serine carboxypeptidase S28 family prot... 29 4.5
At2g25150.1 68415.m03008 transferase family protein similar to 1... 28 7.9
At1g27430.1 68414.m03343 GYF domain-containing protein contains ... 28 7.9
At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PU... 28 7.9
>At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C)
Length = 248
Score = 148 bits (358), Expect = 5e-36
Identities = 69/86 (80%), Positives = 76/86 (88%)
Frame = +3
Query: 252 RQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSG 431
+ SVELYAEKVA RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSG
Sbjct: 81 QDSVELYAEKVANRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSG 140
Query: 432 KLRGQRAKSMKFVDGLMIHSGDPCND 509
KLR RAKSMKF DG M+ SG P +
Sbjct: 141 KLRAARAKSMKFKDGYMVSSGQPTKE 166
Score = 134 bits (325), Expect = 5e-32
Identities = 66/78 (84%), Positives = 71/78 (91%)
Frame = +1
Query: 25 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 204
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 205 RIRELTSVVQKRFNIPDN 258
RIRELTS+VQKRF P +
Sbjct: 65 RIRELTSLVQKRFKFPQD 82
Score = 79.4 bits (187), Expect = 2e-15
Identities = 34/65 (52%), Positives = 46/65 (70%)
Frame = +2
Query: 494 RPLQ*YVNTATRHVLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLE 673
+P + Y++ A RHVLLRQGVLG+KVKIML WD +GK GP P PD +++ PK++ V +
Sbjct: 162 QPTKEYIDAAVRHVLLRQGVLGLKVKIMLDWDPKGKQGPMTPLPDVVIIHTPKEDDVYIA 221
Query: 674 PTSEV 688
P V
Sbjct: 222 PAQVV 226
>At3g53870.1 68416.m05951 40S ribosomal protein S3 (RPS3B) ribosomal
protein S3a - Xenopus laevis, PIR:R3XL3A
Length = 249
Score = 145 bits (351), Expect = 3e-35
Identities = 68/84 (80%), Positives = 74/84 (88%)
Frame = +3
Query: 258 SVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKL 437
SVELYAEKV RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSGKL
Sbjct: 83 SVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKL 142
Query: 438 RGQRAKSMKFVDGLMIHSGDPCND 509
R RAKSMKF DG M+ SG P +
Sbjct: 143 RAARAKSMKFKDGYMVSSGQPTKE 166
Score = 134 bits (324), Expect = 6e-32
Identities = 66/76 (86%), Positives = 70/76 (92%)
Frame = +1
Query: 25 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 204
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 205 RIRELTSVVQKRFNIP 252
RIRELTS+VQKRF P
Sbjct: 65 RIRELTSLVQKRFKFP 80
Score = 77.8 bits (183), Expect = 7e-15
Identities = 33/61 (54%), Positives = 45/61 (73%)
Frame = +2
Query: 494 RPLQ*YVNTATRHVLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLE 673
+P + Y+++A RHVLLRQGVLGIKVK+ML WD +G +GPK P PD +++ PK+E
Sbjct: 162 QPTKEYIDSAVRHVLLRQGVLGIKVKVMLDWDPKGISGPKTPLPDVVIIHSPKEEEAIYA 221
Query: 674 P 676
P
Sbjct: 222 P 222
>At2g31610.1 68415.m03862 40S ribosomal protein S3 (RPS3A)
Length = 250
Score = 145 bits (351), Expect = 3e-35
Identities = 68/84 (80%), Positives = 74/84 (88%)
Frame = +3
Query: 258 SVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGVLRFIMESGARGCEVVVSGKL 437
SVELYAEKV RGLCAIAQAESLRYKL+GGLAVRRACYGVLRF+MESGA+GCEV+VSGKL
Sbjct: 83 SVELYAEKVNNRGLCAIAQAESLRYKLLGGLAVRRACYGVLRFVMESGAKGCEVIVSGKL 142
Query: 438 RGQRAKSMKFVDGLMIHSGDPCND 509
R RAKSMKF DG M+ SG P +
Sbjct: 143 RAARAKSMKFKDGYMVSSGQPTKE 166
Score = 134 bits (324), Expect = 6e-32
Identities = 66/76 (86%), Positives = 70/76 (92%)
Frame = +1
Query: 25 ISKKRKFVGDGVFKAELNEFLTRELAEDGYSGVEVRVTPIRSEIIIMATRTQSVLGEKGR 204
ISKKRKFV DGVF AELNE LTRELAEDGYSGVEVRVTP+R+EIII ATRTQ+VLGEKGR
Sbjct: 5 ISKKRKFVADGVFYAELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGR 64
Query: 205 RIRELTSVVQKRFNIP 252
RIRELTS+VQKRF P
Sbjct: 65 RIRELTSLVQKRFKFP 80
Score = 81.0 bits (191), Expect = 8e-16
Identities = 36/61 (59%), Positives = 45/61 (73%)
Frame = +2
Query: 494 RPLQ*YVNTATRHVLLRQGVLGIKVKIMLPWDQQGKNGPKKPQPDHILVTEPKDEPVPLE 673
+P + Y++ A RHVLLRQGVLGIKVKIML WD GK+GPK P PD +++ PKD+ V
Sbjct: 162 QPTKEYIDAAVRHVLLRQGVLGIKVKIMLDWDPTGKSGPKTPLPDVVIIHAPKDDVVYSA 221
Query: 674 P 676
P
Sbjct: 222 P 222
>At3g06190.2 68416.m00712 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 295
Score = 33.1 bits (72), Expect = 0.21
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -2
Query: 222 SELTDSASFLSEHTLCPGGHNNDLRADGSD 133
SEL + + LSEH+L GH +L ADG D
Sbjct: 255 SELLEYVARLSEHSLTSSGHRKELFADGCD 284
>At3g06190.1 68416.m00711 speckle-type POZ protein-related similar
to SPOP (novel nuclear speckle-type protein)
(SP:O43791) [Homo sapiens]; contains Pfam PF00651 :
BTB/POZ domain; contains Pfam PF00917: MATH domain
Length = 406
Score = 33.1 bits (72), Expect = 0.21
Identities = 15/30 (50%), Positives = 19/30 (63%)
Frame = -2
Query: 222 SELTDSASFLSEHTLCPGGHNNDLRADGSD 133
SEL + + LSEH+L GH +L ADG D
Sbjct: 366 SELLEYVARLSEHSLTSSGHRKELFADGCD 395
>At5g66850.1 68418.m08428 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain; identical
to cDNA MAP3K gamma protein kinase GI:2315152
Length = 716
Score = 30.3 bits (65), Expect = 1.5
Identities = 12/22 (54%), Positives = 17/22 (77%)
Frame = -1
Query: 538 KHMSGSSVDVSLQGSPEWIMSP 473
KH++G D+SL+GSP W M+P
Sbjct: 495 KHLTGQRADLSLKGSPYW-MAP 515
>At1g64070.1 68414.m07258 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 997
Score = 29.1 bits (62), Expect = 3.4
Identities = 18/49 (36%), Positives = 25/49 (51%)
Frame = +3
Query: 231 TEAIQHSRQSVELYAEKVATRGLCAIAQAESLRYKLIGGLAVRRACYGV 377
T+AI+ SR S+ L ++ A+ G C E L+ K G V YGV
Sbjct: 61 TQAIRESRISIVLLSKNYASSGWCLDELLEILKCKDDMGQIVMTVFYGV 109
>At5g19000.1 68418.m02257 speckle-type POZ protein-related contains
Pfam PF00651 : BTB/POZ domain; contains Pfam PF00917:
MATH domain; similar to Speckle-type POZ protein
(SP:O43791) [Homo sapiens]
Length = 442
Score = 28.7 bits (61), Expect = 4.5
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -2
Query: 222 SELTDSASFLSEHTLCPGGHNNDLRADGSD 133
+EL + LSEH++ GH ++ ADG D
Sbjct: 402 TELLQYVARLSEHSVIVSGHRKEIFADGCD 431
>At4g36190.1 68417.m05149 serine carboxypeptidase S28 family protein
contains Pfam PF05577: Serine carboxypeptidase S28
Length = 493
Score = 28.7 bits (61), Expect = 4.5
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +2
Query: 26 FQRSENLLEMGFSRQNSMSSSLGSWPRTATPAWKCGSLPSA 148
F RS N+ F S S +L +W R P CGSL S+
Sbjct: 161 FNRSSNVENPWFFFGVSYSGALSAWFRLKFPHLTCGSLASS 201
>At2g25150.1 68415.m03008 transferase family protein similar to
10-deacetylbaccatin III-10-O-acetyl transferase
[gi:6746554], 2-debenzoyl-7,13-diacetylbaccatin
III-2-O-benzoyl transferase [gi:11559716] from Taxus
cuspidata; contains Pfam transferase family domain
PF00248; contains EST gb:R65039
Length = 461
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = -2
Query: 153 LRADGSDPHFHAGVAVLGQLPSEELIEF 70
L+ SDP GV V+ +LP + ++EF
Sbjct: 418 LKPSKSDPSMEGGVKVIMKLPRDAMVEF 445
>At1g27430.1 68414.m03343 GYF domain-containing protein contains
Pfam profile: PF02213 GYF domain
Length = 1531
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 2/62 (3%)
Frame = -1
Query: 571 FDL--DS*YSLSEKHMSGSSVDVSLQGSPEWIMSPSTNFIDLAR*PRSLPDTTTSQPRAP 398
FDL D +S S + + D S+ SP+W+ + S+ + R P +P S P
Sbjct: 6 FDLPDDLIFSKSSDQLKELASDNSIPLSPQWLYTKSSEYKMDVRSPTPVPMGNPSDPNPK 65
Query: 397 DS 392
D+
Sbjct: 66 DA 67
>At1g09830.1 68414.m01105 phosphoribosylamine--glycine ligase (PUR2)
Identical to phosphoribosylamine--glycine ligase,
chloroplast [precursor] SP:P52420 from [Arabidopsis
thaliana]
Length = 532
Score = 27.9 bits (59), Expect = 7.9
Identities = 12/26 (46%), Positives = 15/26 (57%)
Frame = +3
Query: 399 GARGCEVVVSGKLRGQRAKSMKFVDG 476
G+ GC+VVV L G+ A VDG
Sbjct: 273 GSAGCQVVVEEFLEGEEASFFALVDG 298
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,606,230
Number of Sequences: 28952
Number of extensions: 360107
Number of successful extensions: 1106
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1044
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1106
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1716774400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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