BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00146X (585 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizo... 157 1e-39 SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizo... 156 3e-39 SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 2.0 SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|... 27 2.7 SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr ... 26 3.5 SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 25 8.1 >SPBC1711.06 |rpl401|rpl4-1, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 157 bits (380), Expect = 1e-39 Identities = 82/170 (48%), Positives = 100/170 (58%) Frame = +2 Query: 5 KSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRA 184 K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AGHQTSAESWGTGRA Sbjct: 13 KDGSVSSETLALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRA 72 Query: 185 VARIPRVRGGGTHRSGQGAFGNMFVVDVCSPPRSPGGVGTVASTSDSGERPWRQRCCYRS 364 +ARIPRV GGGTHRSGQ AFGNM P V + Sbjct: 73 LARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKRYAIASAVAASG 132 Query: 365 PSARSX*RTHY*KDSPSFPLVVADKVQEINKTKQAVIFLRRLKAWSDILR 514 + R H ++ P PLVV D VQ KTK+AV L+ +KA+ D+++ Sbjct: 133 VPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVIK 182 Score = 63.3 bits (147), Expect = 3e-11 Identities = 35/110 (31%), Positives = 49/110 (44%) Frame = +1 Query: 256 RGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXQESQRSFXLEDTLLKRFPELPTGCSR 435 R GRMFAPTK WR+WH ++ PE+P Sbjct: 97 RSGRMFAPTKTWRKWHVKVNQNEKRYAIASAVAASGVPSLLLARGHRIEEIPEVPLVVDD 156 Query: 436 QSPGDQQDQTGCHLPEAPQGMV*YLKVYKSQRLRAGKGKMRNRRRIQRKG 585 Q+ + L + + +KV S++LRAGKGK+RNRR +QR+G Sbjct: 157 AVQSFQKTKEAVALLKEIKAYRDVIKVANSRKLRAGKGKLRNRRHVQRRG 206 >SPBP8B7.03c |rpl402|rpl4-2, rpl4|60S ribosomal protein L2|Schizosaccharomyces pombe|chr 2|||Manual Length = 363 Score = 156 bits (378), Expect = 3e-39 Identities = 82/170 (48%), Positives = 100/170 (58%) Frame = +2 Query: 5 KSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRA 184 K +V LPFVFKAPIRPDLV VH +++KN RQPY VS++AGHQTSAESWGTGRA Sbjct: 13 KDGSVSSETIALPFVFKAPIRPDLVRSVHTAVAKNKRQPYAVSEKAGHQTSAESWGTGRA 72 Query: 185 VARIPRVRGGGTHRSGQGAFGNMFVVDVCSPPRSPGGVGTVASTSDSGERPWRQRCCYRS 364 +ARIPRV GGGTHRSGQ AFGNM P V + Sbjct: 73 LARIPRVGGGGTHRSGQAAFGNMCRSGRMFAPTKTWRKWHVKVNQNEKRYAISSAVAASG 132 Query: 365 PSARSX*RTHY*KDSPSFPLVVADKVQEINKTKQAVIFLRRLKAWSDILR 514 + R H ++ P PLVV D VQ KTK+AV L+ +KA+ D+++ Sbjct: 133 VPSLLLARGHRIEEIPEVPLVVDDAVQSFQKTKEAVALLKEIKAYRDVVK 182 Score = 62.9 bits (146), Expect = 3e-11 Identities = 35/110 (31%), Positives = 49/110 (44%) Frame = +1 Query: 256 RGGRMFAPTKPWRRWHXXXXXXXXXXXXXXXXXXQESQRSFXLEDTLLKRFPELPTGCSR 435 R GRMFAPTK WR+WH ++ PE+P Sbjct: 97 RSGRMFAPTKTWRKWHVKVNQNEKRYAISSAVAASGVPSLLLARGHRIEEIPEVPLVVDD 156 Query: 436 QSPGDQQDQTGCHLPEAPQGMV*YLKVYKSQRLRAGKGKMRNRRRIQRKG 585 Q+ + L + + +KV S++LRAGKGK+RNRR +QR+G Sbjct: 157 AVQSFQKTKEAVALLKEIKAYRDVVKVANSRKLRAGKGKLRNRRHVQRRG 206 >SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 1611 Score = 27.1 bits (57), Expect = 2.0 Identities = 12/42 (28%), Positives = 17/42 (40%) Frame = +3 Query: 267 YVRPHEALAALAPSRQPPTAESGLGGSVAATGVPALVXARGH 392 + PH L + AP QP E +V P+ +GH Sbjct: 854 FYEPHSYLESPAPEPQPSYEEESFNATVIHAPTPSTATFQGH 895 >SPBC28F2.12 |rpb1||DNA-directed RNA polymerase II large subunit|Schizosaccharomyces pombe|chr 2|||Manual Length = 1752 Score = 26.6 bits (56), Expect = 2.7 Identities = 16/53 (30%), Positives = 22/53 (41%) Frame = +2 Query: 170 GTGRAVARIPRVRGGGTHRSGQGAFGNMFVVDVCSPPRSPGGVGTVASTSDSG 328 G+ A A P V+GG R G G +G + +SPG +S G Sbjct: 1498 GSPDAAAFSPLVQGGSEGREGFGDYGLLGAASPYKGVQSPGYTSPFSSAMSPG 1550 >SPAC11E3.02c |||C2 domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1237 Score = 26.2 bits (55), Expect = 3.5 Identities = 22/89 (24%), Positives = 35/89 (39%), Gaps = 7/89 (7%) Frame = +2 Query: 17 VQGAAKPLPFVFKAPIRPDLVNDVHVSM-------SKNSRQPYCVSKEAGHQTSAESWGT 175 + G+ + LPF F +P L+N++H +M K Q + + T Sbjct: 745 LSGSNEVLPFRF-SPTMCILLNNIHFAMHAYEELEQKIDLQRLIEALDVAESTKRHKISA 803 Query: 176 GRAVARIPRVRGGGTHRSGQGAFGNMFVV 262 + I VRG G H G G ++V Sbjct: 804 TNYLYTIKVVRGEGLHPDGAGKIRTSYIV 832 >SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.0 bits (52), Expect = 8.1 Identities = 22/95 (23%), Positives = 39/95 (41%), Gaps = 7/95 (7%) Frame = +2 Query: 71 DLVNDVHVSMSKNSRQPYCVSKEAGHQTSAESWGTGRAVARIPRVRGGGTHR-------S 229 DLV+ + N + P GH S +S GR + + + + S Sbjct: 161 DLVSVPEIFEQSNPK-PVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANS 219 Query: 230 GQGAFGNMFVVDVCSPPRSPGGVGTVASTSDSGER 334 G+G+F +++D R+ G VAST+ ++ Sbjct: 220 GKGSFSYAWLLDTTEEERARGVTMDVASTTFESDK 254 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,360,056 Number of Sequences: 5004 Number of extensions: 45355 Number of successful extensions: 127 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 115 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 252150250 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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