BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00142 (842 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g38150.1 68415.m04683 alpha 1,4-glycosyltransferase family pr... 29 3.9 At3g59100.1 68416.m06589 glycosyl transferase family 48 protein ... 29 5.1 At3g23920.1 68416.m03005 beta-amylase, putative / 1,4-alpha-D-gl... 29 5.1 At2g38185.2 68415.m04690 zinc finger (C3HC4-type RING finger) fa... 28 6.8 At2g38185.1 68415.m04689 zinc finger (C3HC4-type RING finger) fa... 28 6.8 At5g37870.1 68418.m04561 seven in absentia (SINA) family protein... 28 8.9 >At2g38150.1 68415.m04683 alpha 1,4-glycosyltransferase family protein / glycosyltransferase sugar-binding DXD motif-containing protein low similarity to alpha-1,4-N-acetylglucosaminyltransferase, Homo sapiens [gi:5726306], Gb3 synthase, Rattus norvegicus [gi:9082162] ; contains Pfam profiles PF04572: Alpha 1,4-glycosyltransferase conserved region, PF04488: Glycosyltransferase sugar-binding region containing DXD motif Length = 736 Score = 29.1 bits (62), Expect = 3.9 Identities = 15/39 (38%), Positives = 21/39 (53%) Frame = -2 Query: 568 NSCGFTSLSEYLGIQEFRGKTSAKFLTLTFLVSRLCISA 452 ++C SLSE L +++FR S F T F V L + A Sbjct: 4 DNCNKKSLSEVLDVRQFRHTNSPVFSTAIFAVIMLLVVA 42 >At3g59100.1 68416.m06589 glycosyl transferase family 48 protein contains Pfam profile: PF02364 1,3-beta-glucan synthase Length = 1934 Score = 28.7 bits (61), Expect = 5.1 Identities = 10/24 (41%), Positives = 15/24 (62%), Gaps = 2/24 (8%) Frame = +2 Query: 674 INLH--HYHWHLVYPFTANDLSIV 739 +NLH HY WH +P N++ +V Sbjct: 715 MNLHIGHYQWHEFFPHATNNIGVV 738 >At3g23920.1 68416.m03005 beta-amylase, putative / 1,4-alpha-D-glucan maltohydrolase, putative similar to beta-amylase enzyme [Arabidopsis thaliana] GI:6065749, beta-amylase PCT-BMYI from [Solanum tuberosum]; contains Pfam profile PF01373: Glycosyl hydrolase family 14 Length = 575 Score = 28.7 bits (61), Expect = 5.1 Identities = 16/58 (27%), Positives = 32/58 (55%), Gaps = 2/58 (3%) Frame = +3 Query: 267 IATQLPKDAEFSLFLPKHQEMANE--LLGVLMDVPENELQDLLSTCAFARVNLNPQLF 434 +A ++P E +L P++ + A+E L +++ +N + CAF + +NP+LF Sbjct: 468 LAAEVPLAGENAL--PRYDDYAHEQILKASALNLDQNNEGEPREMCAFTYLRMNPELF 523 >At2g38185.2 68415.m04690 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 441 Score = 28.3 bits (60), Expect = 6.8 Identities = 17/62 (27%), Positives = 31/62 (50%) Frame = +3 Query: 438 YCYSVALMHRRDTRKVRVKNFAEVFPLNSWIPKYSLKLVKPQLLFHQTFHAYLLSFHETT 617 YC+S+AL+ + ++N P N W+ S LV+P +F ++ +L + E Sbjct: 63 YCFSLALLIWFFASFILIENLYG--PKNVWLGPSSSILVEPSSIFVKSIKVKVLDYSEPG 120 Query: 618 LR 623 L+ Sbjct: 121 LQ 122 >At2g38185.1 68415.m04689 zinc finger (C3HC4-type RING finger) family protein contains Pfam profile PF00097: Zinc finger, C3HC4 type (RING finger) Length = 441 Score = 28.3 bits (60), Expect = 6.8 Identities = 17/62 (27%), Positives = 31/62 (50%) Frame = +3 Query: 438 YCYSVALMHRRDTRKVRVKNFAEVFPLNSWIPKYSLKLVKPQLLFHQTFHAYLLSFHETT 617 YC+S+AL+ + ++N P N W+ S LV+P +F ++ +L + E Sbjct: 63 YCFSLALLIWFFASFILIENLYG--PKNVWLGPSSSILVEPSSIFVKSIKVKVLDYSEPG 120 Query: 618 LR 623 L+ Sbjct: 121 LQ 122 >At5g37870.1 68418.m04561 seven in absentia (SINA) family protein similar to SIAH1 protein [Brassica napus var. napus] GI:7657876; contains Pfam profile PF03145: Seven in absentia protein family Length = 281 Score = 27.9 bits (59), Expect = 8.9 Identities = 17/52 (32%), Positives = 28/52 (53%) Frame = +3 Query: 231 TPQEPQQTPEFKIATQLPKDAEFSLFLPKHQEMANELLGVLMDVPENELQDL 386 T Q P+ K+++Q PKD S L H + LLG+++ + NEL+ + Sbjct: 235 TYQSPKVKKVLKVSSQRPKD---SFMLIPHSLLCGPLLGMMLCI--NELKQM 281 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,357,275 Number of Sequences: 28952 Number of extensions: 353156 Number of successful extensions: 907 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 880 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 907 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1950880000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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