Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= heS00138
         (419 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...   134   7e-33
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc...    79   2e-16
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch...    73   2e-14
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    66   2e-12
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc...    50   2e-07
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc...    48   6e-07
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom...    48   8e-07
SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...    27   1.2  
SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting...    25   3.6  
SPCC285.05 |||purine nucleoside transporter |Schizosaccharomyces...    25   4.8  
SPCC1235.08c |pdh1||DUF1751 family protein|Schizosaccharomyces p...    25   6.3  
SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein |Schizosacch...    25   6.3  

>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score =  134 bits (323), Expect = 7e-33
 Identities = 62/92 (67%), Positives = 74/92 (80%)
 Frame = +3

Query: 3   QSGGVRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDL 182
           QSGGVRPFGVSLL+ G +E  P L+Q DPSG YFAWKATA+GK+    KTFLEKRY ++L
Sbjct: 123 QSGGVRPFGVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDEL 182

Query: 183 ELDDAVHTAILTLKEGFEGQMTADT*KLASVT 278
           ELDDAVHTAIL LKE FEG++T D  ++A V+
Sbjct: 183 ELDDAVHTAILALKETFEGELTEDNIEIAVVS 214


>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 259

 Score = 79.4 bits (187), Expect = 2e-16
 Identities = 37/77 (48%), Positives = 52/77 (67%), Gaps = 2/77 (2%)
 Frame = +3

Query: 3   QSGGVRPFGVSLLICGWEEG--RPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTE 176
           QSGGVRPFGVS LI G++ G   P ++Q +P+G Y AWKATA+G+     + +LEK + E
Sbjct: 121 QSGGVRPFGVSTLIAGFDVGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNWKE 180

Query: 177 DLELDDAVHTAILTLKE 227
            L  D+ +H A+ +L E
Sbjct: 181 GLSRDETIHLAVSSLLE 197


>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
           3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 248

 Score = 72.9 bits (171), Expect = 2e-14
 Identities = 35/95 (36%), Positives = 59/95 (62%), Gaps = 2/95 (2%)
 Frame = +3

Query: 3   QSGGVRPFGVSLLICGWEEGRPY-LFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTED 179
           Q GG+RPFGVS L  GW+  R Y LFQ +PSG Y +W+A ++G N  + ++ + + Y +D
Sbjct: 123 QYGGLRPFGVSFLYAGWDHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYKDD 182

Query: 180 LELDDAVHTAILTLKEGFE-GQMTADT*KLASVTR 281
           + LD+A   A+  L +  +   +T +  + A++T+
Sbjct: 183 INLDEASAMAVKFLSKTLDSNSLTHEKIEFATITK 217


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 66.5 bits (155), Expect = 2e-12
 Identities = 33/90 (36%), Positives = 53/90 (58%)
 Frame = +3

Query: 18  RPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDA 197
           RPFGV+LLI G +E  P L+  +PSG YF ++A A+G      K+ L K + +D+ L++A
Sbjct: 136 RPFGVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEA 195

Query: 198 VHTAILTLKEGFEGQMTADT*KLASVTRAG 287
               +  L++  E ++ +   +LA VT  G
Sbjct: 196 EVLILKVLRQVMEEKLDSKNVQLAKVTAEG 225


>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
           Pre5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 49.6 bits (113), Expect = 2e-07
 Identities = 22/77 (28%), Positives = 42/77 (54%), Gaps = 2/77 (2%)
 Frame = +3

Query: 3   QSGGVRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYT--E 176
           Q  G RP+GV  L+ G++E  P+L +  PSG    +  T+MG    + +T++E+      
Sbjct: 121 QEYGRRPYGVGFLVIGYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFP 180

Query: 177 DLELDDAVHTAILTLKE 227
           D   ++ + +A+  L++
Sbjct: 181 DSSREELILSALRALRD 197


>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 244

 Score = 48.0 bits (109), Expect = 6e-07
 Identities = 29/77 (37%), Positives = 41/77 (53%), Gaps = 4/77 (5%)
 Frame = +3

Query: 3   QSGGVRPFGVSL-LICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTED 179
           Q   +RP GV++ L+   +E  P LF+ DP+G Y  +KAT+ G        +LEKR+ +D
Sbjct: 125 QRAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKRFKKD 184

Query: 180 ---LELDDAVHTAILTL 221
                L D V T I  L
Sbjct: 185 GIPSNLTDTVETGIQAL 201


>SPCC1795.04c |||20S proteasome component alpha
           7|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 253

 Score = 47.6 bits (108), Expect = 8e-07
 Identities = 22/65 (33%), Positives = 34/65 (52%)
 Frame = +3

Query: 15  VRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDD 194
           VRPFGV   +  ++   P+L+  +P+G Y+ +   A GK     +  LEK     L++ D
Sbjct: 128 VRPFGVMSFVATYDSEGPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEKLNFSSLKMKD 187

Query: 195 AVHTA 209
           AV  A
Sbjct: 188 AVKEA 192


>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score = 27.1 bits (57), Expect = 1.2
 Identities = 21/76 (27%), Positives = 33/76 (43%)
 Frame = +3

Query: 27  GVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDAVHT 206
           G  L++ G++   P+LF     G+       A+G       + LE +Y  DLE     H 
Sbjct: 130 GAYLVLGGYDCKGPHLFTIAAHGSSDKLPYVALGSGSLAAISVLETKYQPDLER----HE 185

Query: 207 AILTLKEGFEGQMTAD 254
           A+  +KE  E  +  D
Sbjct: 186 AMELVKEAIEAGIFND 201


>SPAC19G12.01c |cut20|lid1, apc4, SPAPJ698.04c|anaphase-promoting
           complex subunit Apc4|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 719

 Score = 25.4 bits (53), Expect = 3.6
 Identities = 13/41 (31%), Positives = 20/41 (48%)
 Frame = -3

Query: 153 KFFHY*SSFPWLWLSMQNRLRRGHTGTGKDVLLPSHRSIEI 31
           K F Y     + W++  NR+ R  +G  + +L PS   I I
Sbjct: 4   KSFKYPKKHKFEWINETNRVERVGSGLKRVILCPSMELIAI 44


>SPCC285.05 |||purine nucleoside transporter |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 348

 Score = 25.0 bits (52), Expect = 4.8
 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 3/61 (4%)
 Frame = +3

Query: 66  PYLFQCDPSGAYFAWKATAMG---KNFNNGKTFLEKRYTEDLELDDAVHTAILTLKEGFE 236
           P++ QCD +     W  T MG    N  N  T     Y    + D+A  TA+   +  F+
Sbjct: 227 PFITQCDTATGDNYWAGTYMGDFVSNITNVLTNSTGHYCTTQQEDNASLTAL--TRASFD 284

Query: 237 G 239
           G
Sbjct: 285 G 285


>SPCC1235.08c |pdh1||DUF1751 family protein|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 226

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 9/28 (32%), Positives = 15/28 (53%)
 Frame = -3

Query: 189 LAPSLLYIFFQGKFFHY*SSFPWLWLSM 106
           L+  L+Y+F     F+   SFPW +  +
Sbjct: 166 LSKYLIYLFLSIHLFYVFQSFPWTYFCL 193


>SPAC18B11.11 ||SPAC1F5.01|GTPase activating protein
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1294

 Score = 24.6 bits (51), Expect = 6.3
 Identities = 18/49 (36%), Positives = 22/49 (44%)
 Frame = -1

Query: 263  LLCICSHLTFKTFLKGQDSCVNCVI*LQVFCISFFKESFSIIKVLSHGC 117
            L C+    T K  L G    VN     +VFC+     S+SII  L  GC
Sbjct: 862  LFCLKVIRTKKEELDGHKKLVNPNT--KVFCLYLVSFSYSIISNLFLGC 908


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,820,409
Number of Sequences: 5004
Number of extensions: 37688
Number of successful extensions: 88
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 85
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 86
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 148351622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)