Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00138
(419 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 142 7e-35
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 142 7e-35
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 72 1e-13
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 72 2e-13
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 70 6e-13
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 66 1e-11
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 64 3e-11
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 60 8e-10
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 57 4e-09
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 54 3e-08
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 54 4e-08
At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid t... 47 5e-06
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 42 2e-04
At3g04370.1 68416.m00462 hypothetical protein contains Pfam prof... 27 3.9
At3g17770.1 68416.m02267 dihydroxyacetone kinase family protein ... 26 9.0
At2g17230.1 68415.m01990 phosphate-responsive 1 family protein s... 26 9.0
At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15) ... 26 9.0
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 142 bits (345), Expect = 7e-35
Identities = 61/91 (67%), Positives = 79/91 (86%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDL 182
QSGGVRPFGVSLL+ G+++ P L+Q DPSG+YF+WKA+AMGKN +N KTFLEKRYTED+
Sbjct: 123 QSGGVRPFGVSLLVAGYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDM 182
Query: 183 ELDDAVHTAILTLKEGFEGQMTADT*KLASV 275
ELDDA+HTAILTLKEGFEG++++ ++ +
Sbjct: 183 ELDDAIHTAILTLKEGFEGEISSKNIEIGKI 213
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 142 bits (345), Expect = 7e-35
Identities = 61/91 (67%), Positives = 79/91 (86%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDL 182
QSGGVRPFGVSLL+ G+++ P L+Q DPSG+YF+WKA+AMGKN +N KTFLEKRYTED+
Sbjct: 123 QSGGVRPFGVSLLVAGYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDM 182
Query: 183 ELDDAVHTAILTLKEGFEGQMTADT*KLASV 275
ELDDA+HTAILTLKEGFEG++++ ++ +
Sbjct: 183 ELDDAIHTAILTLKEGFEGEISSKNIEIGKI 213
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 72.1 bits (169), Expect = 1e-13
Identities = 40/80 (50%), Positives = 49/80 (61%), Gaps = 2/80 (2%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWEEGR--PYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTE 176
QSGGVRPFG+S LI G++ P L+Q DPSG + AWKA A G+N N+ + FLEK Y E
Sbjct: 121 QSGGVRPFGLSTLIVGFDPYTRIPALYQTDPSGTFSAWKANATGRNSNSIREFLEKNYKE 180
Query: 177 DLELDDAVHTAILTLKEGFE 236
+ V AI L E E
Sbjct: 181 SAG-QETVKLAIRALLEVVE 199
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 71.7 bits (168), Expect = 2e-13
Identities = 39/80 (48%), Positives = 49/80 (61%), Gaps = 2/80 (2%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWE--EGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTE 176
QSGGVRPFG+S LI G++ P L+Q DPSG + AWKA A G+N N+ + FLEK Y E
Sbjct: 121 QSGGVRPFGLSTLIVGFDPYSRLPSLYQTDPSGTFSAWKANATGRNSNSIREFLEKNYKE 180
Query: 177 DLELDDAVHTAILTLKEGFE 236
+ + AI L E E
Sbjct: 181 S-SGQETIKLAIRALLEVVE 199
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 70.1 bits (164), Expect = 6e-13
Identities = 35/93 (37%), Positives = 55/93 (59%), Gaps = 2/93 (2%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWEEGRPY-LFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTED 179
Q GG+RPFGVS L GW++ + L+ DPSG Y WKA A+G N ++ L++ Y +D
Sbjct: 123 QFGGLRPFGVSFLFAGWDKHHGFQLYMSDPSGNYGGWKAAAVGANNQAAQSILKQDYKDD 182
Query: 180 LELDDAVHTAILTLKEGFEG-QMTADT*KLASV 275
++AV A+ L + + +T++ +LA V
Sbjct: 183 ATREEAVELALKVLTKTMDSTSLTSEKLELAEV 215
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 65.7 bits (153), Expect = 1e-11
Identities = 33/89 (37%), Positives = 51/89 (57%)
Frame = +3
Query: 18 RPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDA 197
RPFGVSLLI G +E P L+ DPSG ++ A A+G + L++++ +DL L +A
Sbjct: 133 RPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDLSLQEA 192
Query: 198 VHTAILTLKEGFEGQMTADT*KLASVTRA 284
A+ LK+ E ++T + +A V A
Sbjct: 193 ETIAVSILKQVMEEKVTPNNVDIAKVAPA 221
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 64.5 bits (150), Expect = 3e-11
Identities = 32/89 (35%), Positives = 51/89 (57%)
Frame = +3
Query: 18 RPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDA 197
RPFGVSLLI G +E P L+ DPSG ++ A A+G + L++++ +D+ L +A
Sbjct: 133 RPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDITLQEA 192
Query: 198 VHTAILTLKEGFEGQMTADT*KLASVTRA 284
A+ LK+ E ++T + +A V A
Sbjct: 193 ETIAVSILKQVMEEKVTPNNVDIAKVAPA 221
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 59.7 bits (138), Expect = 8e-10
Identities = 29/77 (37%), Positives = 46/77 (59%), Gaps = 2/77 (2%)
Frame = +3
Query: 18 RPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTE--DLELD 191
RP+GV LL+ G +E +L+ PSG YF ++A A+G KT+LE+R+ D +
Sbjct: 126 RPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFGDSSRE 185
Query: 192 DAVHTAILTLKEGFEGQ 242
D + AIL ++E +G+
Sbjct: 186 DLIKDAILAVRETLQGE 202
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 57.2 bits (132), Expect = 4e-09
Identities = 26/77 (33%), Positives = 47/77 (61%), Gaps = 2/77 (2%)
Frame = +3
Query: 18 RPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYT--EDLELD 191
RP+GV LL+ G +E +L+ PSG YF ++A A+G KT+LE+++ ++ +
Sbjct: 126 RPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQESSKE 185
Query: 192 DAVHTAILTLKEGFEGQ 242
D + AI+ ++E +G+
Sbjct: 186 DLIKDAIMAIRETLQGE 202
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 54.4 bits (125), Expect = 3e-08
Identities = 29/77 (37%), Positives = 43/77 (55%), Gaps = 3/77 (3%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGWEEGR-PYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTED 179
Q +RP GV ++ G +E R P L++CDP+G ++ KAT+ G FLEK+ E+
Sbjct: 127 QHAYMRPLGVVAMVLGIDEERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKEN 186
Query: 180 --LELDDAVHTAILTLK 224
D+ V TAI L+
Sbjct: 187 PAFTYDETVQTAISALQ 203
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 54.0 bits (124), Expect = 4e-08
Identities = 29/77 (37%), Positives = 42/77 (54%), Gaps = 3/77 (3%)
Frame = +3
Query: 3 QSGGVRPFGVSLLICGW-EEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTED 179
Q +RP GV ++ G EE P L++CDP+G ++ KAT+ G FLEK+ E+
Sbjct: 127 QHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKEN 186
Query: 180 --LELDDAVHTAILTLK 224
D+ V TAI L+
Sbjct: 187 PSFTFDETVQTAISALQ 203
>At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
SP|Q00451|PRF1_LYCES 36.4 kDa proline-rich protein
Lycopersicon esculentum, proline-rich cell wall protein
[Medicago sativa] GI:3818416; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 428
Score = 47.2 bits (107), Expect = 5e-06
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = +3
Query: 30 VSLLICGWEEGRPY-LFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDAVHT 206
V+ + GW+ + L+ DPSG Y W+A A+G N ++ L++ Y +D ++ V
Sbjct: 354 VACAVAGWDRNHGFQLYMSDPSGNYGGWQAAAVGANNQAAQSILKQDYKDDATREEVVQL 413
Query: 207 AILTLKE 227
AI L +
Sbjct: 414 AIKVLSK 420
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 41.9 bits (94), Expect = 2e-04
Identities = 18/62 (29%), Positives = 33/62 (53%)
Frame = +3
Query: 15 VRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDD 194
+RPFG +++ G++ P L+ +PSG + + A+GK KT +EK ++ +
Sbjct: 129 LRPFGCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGKQAAKTEIEKLNLSEMTCKE 188
Query: 195 AV 200
V
Sbjct: 189 GV 190
>At3g04370.1 68416.m00462 hypothetical protein contains Pfam profile
PF01657: Domain of unknown function
Length = 332
Score = 27.5 bits (58), Expect = 3.9
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = +3
Query: 78 QCDPSGAYFAWKATAMGKNFNNGKTFL 158
QCDP A KAT N++N FL
Sbjct: 38 QCDPLDANILQKATTKSPNYSNQNLFL 64
>At3g17770.1 68416.m02267 dihydroxyacetone kinase family protein
contains Pfam domains, PF02733: DAK1 domain and PF02734:
DAK2 domain
Length = 595
Score = 26.2 bits (55), Expect = 9.0
Identities = 13/23 (56%), Positives = 15/23 (65%)
Frame = +2
Query: 68 LPVPV*PLRSLFCMESHSHGKEL 136
+PVPV P RS+ MES S EL
Sbjct: 354 IPVPVPPSRSIKSMESQSRPLEL 376
>At2g17230.1 68415.m01990 phosphate-responsive 1 family protein
similar to phi-1 (phosphate-induced gene) [Nicotiana
tabacum] GI:3759184; contains Pfam profile PF04674:
Phosphate-induced protein 1 conserved region
Length = 363
Score = 26.2 bits (55), Expect = 9.0
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -3
Query: 186 APSLLYIFFQGKFFHY*SSFPWLWLSMQNRLRRGHTGTGKDVLLPS 49
+P+ FF FF SSF ++ S+QN HT T + +PS
Sbjct: 3 SPATTITFF---FFFTLSSFFYITSSLQNNNNNKHTATVNSLNIPS 45
>At1g17110.1 68414.m02085 ubiquitin-specific protease 15 (UBP15)
almost identical to ubiquitin-specific protease 15
GI:11993475 [Arabidopsis thaliana], 7 amino acid
difference
Length = 924
Score = 26.2 bits (55), Expect = 9.0
Identities = 13/43 (30%), Positives = 20/43 (46%)
Frame = -1
Query: 218 GQDSCVNCVI*LQVFCISFFKESFSIIKVLSHGCGFPCKIGSG 90
G+D C+ C + V + S ++LSH C+IG G
Sbjct: 477 GKDWCLMCELEQHVMMLRESGGPLSASRILSHMRSINCQIGDG 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,698,993
Number of Sequences: 28952
Number of extensions: 208006
Number of successful extensions: 530
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 517
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 527
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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