BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00130 (697 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pomb... 39 6e-04 SPAC3A12.18 |zwf1|SPAC9.01|glucose-6-phosphate 1-dehydrogenase |... 27 3.4 SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 26 4.5 SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex lar... 26 4.5 SPBC3B9.22c |dad4||DASH complex subunit Dad4|Schizosaccharomyces... 26 4.5 SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|S... 26 4.5 >SPBPB2B2.12c |||UDP-glucose 4-epimerase|Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 39.1 bits (87), Expect = 6e-04 Identities = 23/62 (37%), Positives = 30/62 (48%), Gaps = 6/62 (9%) Frame = +2 Query: 341 FSIDGTTYQLANNIG-KDHLHGGINGFNKANWNSTV-----DGTKVIFSYLSKDGEEGYP 502 F +DG Y L N K LHGG NGF+K + + D + F + KDG G+P Sbjct: 433 FEVDGHLYTLCKNENNKTTLHGGNNGFDKQFFLGPIARQYEDYNTLEFILVDKDGNNGFP 492 Query: 503 GD 508 D Sbjct: 493 SD 494 Score = 26.2 bits (55), Expect = 4.5 Identities = 13/35 (37%), Positives = 18/35 (51%), Gaps = 1/35 (2%) Frame = +3 Query: 246 SGLDELDGNVNRNTPHLGTTVGRCANRI-GGQISV 347 +G ++ + P G T+GR ANRI GQ V Sbjct: 401 NGFNDFSRYKLKENPFFGATIGRFANRIANGQFEV 435 >SPAC3A12.18 |zwf1|SPAC9.01|glucose-6-phosphate 1-dehydrogenase |Schizosaccharomyces pombe|chr 1|||Manual Length = 500 Score = 26.6 bits (56), Expect = 3.4 Identities = 9/21 (42%), Positives = 14/21 (66%) Frame = -2 Query: 558 DMQGIVLGNFISNVSNKSPGY 496 D++ IVLG ++ + K PGY Sbjct: 283 DLKDIVLGQYVKSKDGKKPGY 303 >SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1666 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/31 (35%), Positives = 20/31 (64%) Frame = -2 Query: 681 SVYNKDMVVDLLDTSFVASQIKIRMVCQIYN 589 +VYN + V +LL + +A Q+ + +VC Y+ Sbjct: 749 NVYNPEKVKNLLKEAKLADQLPLILVCDRYD 779 >SPBC4C3.05c |nuc1|rpa1|DNA-directed RNA polymerase I complex large subunit Nuc1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1689 Score = 26.2 bits (55), Expect = 4.5 Identities = 17/45 (37%), Positives = 25/45 (55%) Frame = -2 Query: 534 NFISNVSNKSPGYPSSPSFDR*LNITLVPSTVEFQFALLNPLIPP 400 N + N +K PG + D L I+L+P T+E + AL N L+ P Sbjct: 529 NAVINGPHKWPGASHIQNEDGTL-ISLMPLTIEQRTALANQLLTP 572 >SPBC3B9.22c |dad4||DASH complex subunit Dad4|Schizosaccharomyces pombe|chr 2|||Manual Length = 72 Score = 26.2 bits (55), Expect = 4.5 Identities = 11/17 (64%), Positives = 13/17 (76%) Frame = +1 Query: 52 RLNHSF*IINMVTLNVE 102 RLNHS +INM +NVE Sbjct: 29 RLNHSLQLINMSNMNVE 45 >SPAC3A11.14c |pkl1|klp1, SPAC3H5.03c|kinesin-like protein Pkl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 832 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/24 (41%), Positives = 16/24 (66%) Frame = +1 Query: 124 ETVSKFTWRASDGFSVSVISYGAT 195 E +S+ A DG++VS+ +YG T Sbjct: 554 EEISQLIQSAIDGYNVSIFAYGQT 577 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,940,863 Number of Sequences: 5004 Number of extensions: 62227 Number of successful extensions: 150 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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