Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00124X
(524 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) simi... 103 6e-23
At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S ... 102 1e-22
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 98 3e-21
At2g02640.1 68415.m00203 DC1 domain-containing protein contain... 31 0.47
At1g05440.1 68414.m00552 expressed protein ; expression supporte... 30 1.1
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 29 1.4
At1g16850.1 68414.m02033 expressed protein 29 1.4
At4g03470.1 68417.m00474 ankyrin repeat family protein contains ... 29 2.5
At3g28415.1 68416.m03551 P-glycoprotein, putative contains ATP-b... 28 3.3
At3g28390.1 68416.m03547 P-glycoprotein, putative similar to P-g... 28 3.3
At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pf... 28 3.3
At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to hom... 28 3.3
At5g46840.1 68418.m05771 RNA recognition motif (RRM)-containing ... 28 4.4
At4g35730.1 68417.m05071 expressed protein contains Pfam profil... 27 5.8
At1g32190.1 68414.m03959 expressed protein 27 5.8
At3g28380.1 68416.m03546 P-glycoprotein, putative similar to P-g... 27 7.7
At3g28345.1 68416.m03541 ABC transporter family protein similar ... 27 7.7
>At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) similar
to GB:P42791
Length = 187
Score = 103 bits (248), Expect = 6e-23
Identities = 51/87 (58%), Positives = 65/87 (74%)
Frame = +2
Query: 263 IAVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVL 442
IAV+VGT+T+D+R+++IP M V AL TE+ARARI AGGE LTFDQLALRAP G+ TVL
Sbjct: 78 IAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNTVL 137
Query: 443 VQGQRNAREADASLWPCSRSSASHTKP 523
++G +N+REA P SH+KP
Sbjct: 138 LRGPKNSREAVKHFGPAPGVPHSHSKP 164
Score = 57.2 bits (132), Expect = 6e-09
Identities = 29/71 (40%), Positives = 41/71 (57%), Gaps = 1/71 (1%)
Frame = +3
Query: 30 MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 206
MGID I +K +RT KS D+ TN+KFN ++L+RLFMS++N+
Sbjct: 1 MGIDLIAGGKSKKTKRTAPKSDDVYLKLTVKLYRFLVRRTNSKFNGVILKRLFMSKVNKA 60
Query: 207 PISVSRLARHM 239
P+S+SRL M
Sbjct: 61 PLSLSRLVEFM 71
>At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S
ribosomal protein L18, Arabidopsis thaliana,
SWISSPROT:RL18_ARATH
Length = 187
Score = 102 bits (245), Expect = 1e-22
Identities = 51/87 (58%), Positives = 65/87 (74%)
Frame = +2
Query: 263 IAVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVL 442
IAV+VGT+T+D+R+++IP M V AL TE+ARARI AGGE LTFDQLALRAP G+ TVL
Sbjct: 78 IAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAGGECLTFDQLALRAPLGQNTVL 137
Query: 443 VQGQRNAREADASLWPCSRSSASHTKP 523
++G +N+REA P S+TKP
Sbjct: 138 LRGPKNSREAVKHFGPAPGVPHSNTKP 164
Score = 54.0 bits (124), Expect = 6e-08
Identities = 27/71 (38%), Positives = 40/71 (56%), Gaps = 1/71 (1%)
Frame = +3
Query: 30 MGID-INHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRP 206
MGID I +K +RT KS D+ +N+ FN ++L+RLFMS++N+
Sbjct: 1 MGIDLIAGGKSKKTKRTAPKSDDVYLKLLVKLYRFLVRRSNSNFNAVILKRLFMSKVNKA 60
Query: 207 PISVSRLARHM 239
P+S+SRL M
Sbjct: 61 PLSLSRLVEFM 71
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 98.3 bits (234), Expect = 3e-21
Identities = 52/90 (57%), Positives = 61/90 (67%), Gaps = 1/90 (1%)
Frame = +2
Query: 257 GLIAVVVGTVTNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPT-GKK 433
G IAV+VGTVT+DVR+ +P +TV AL TE ARARI AGGE LTFDQLAL PT +
Sbjct: 23 GKIAVIVGTVTDDVRIEDVPALTVTALRFTESARARIHKAGGECLTFDQLALPCPTWSEN 82
Query: 434 TVLVQGQRNAREADASLWPCSRSSASHTKP 523
TVL++G +N REA P SHTKP
Sbjct: 83 TVLLRGPKNTREAVKHFGPAPGVPHSHTKP 112
Score = 28.3 bits (60), Expect = 3.3
Identities = 10/18 (55%), Positives = 16/18 (88%)
Frame = +3
Query: 186 MSRINRPPISVSRLARHM 239
MS++N+ P+S+SRL R+M
Sbjct: 1 MSKVNKAPLSLSRLVRYM 18
>At2g02640.1 68415.m00203 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 627
Score = 31.1 bits (67), Expect = 0.47
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +1
Query: 331 CSSCYRKSSCTHFGCWRRNSYF 396
CS+C RKS+ + C+ RN YF
Sbjct: 423 CSACMRKSNGFGYSCYNRNCYF 444
>At1g05440.1 68414.m00552 expressed protein ; expression supported
by MPSS
Length = 393
Score = 29.9 bits (64), Expect = 1.1
Identities = 15/42 (35%), Positives = 25/42 (59%)
Frame = -2
Query: 475 ICLTSISLTLYQYCLLASRSTKSQLIKSKNFSSSSQNACTSF 350
ICL S++ TL L +S+ ++L + NF S+ ++ TSF
Sbjct: 70 ICLDSLNATLDLMPLSVQKSSLTKLSSASNFKSTVESTPTSF 111
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 29.5 bits (63), Expect = 1.4
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +2
Query: 383 EILTFDQLALRAPTGKKTVLVQGQRNAREADASL 484
E L FD L L+ P+GK LV G + + SL
Sbjct: 290 ETLIFDDLCLKIPSGKTVALVGGSGSGKSTVISL 323
>At1g16850.1 68414.m02033 expressed protein
Length = 152
Score = 29.5 bits (63), Expect = 1.4
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +3
Query: 405 WLFVLRLARRQYWYKVSEMLVRQMRHFGP 491
WL ++ + + YW KV E L R HF P
Sbjct: 50 WLTKIKRSGKNYWAKVRETLDRGQSHFFP 78
>At4g03470.1 68417.m00474 ankyrin repeat family protein contains
ankyrin repeats, Pfam domain PF00023
Length = 683
Score = 28.7 bits (61), Expect = 2.5
Identities = 17/63 (26%), Positives = 30/63 (47%)
Frame = -2
Query: 325 RHLRYLVQSHVICDCPHYHGNQTLRVGFFMWRAKRDTEIGGRLIRLIKSRRRTI*LNLAF 146
RHL Q + + + GN L + WR + E+GG+ LI++ + L++A
Sbjct: 389 RHLTAHEQINHLANEKDVDGNTPLHLATIYWRPRAVRELGGKKNLLIQNNNGLVALDIAE 448
Query: 145 VRL 137
+L
Sbjct: 449 SKL 451
>At3g28415.1 68416.m03551 P-glycoprotein, putative contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1221
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +2
Query: 395 FDQLALRAPTGKKTVLVQGQRNAREADASL 484
FD L LR P+GK LV G + + SL
Sbjct: 356 FDDLCLRIPSGKSVALVGGSGSGKSTVISL 385
>At3g28390.1 68416.m03547 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1225
Score = 28.3 bits (60), Expect = 3.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +2
Query: 395 FDQLALRAPTGKKTVLVQGQRNAREADASL 484
FD L LR P+GK LV G + + SL
Sbjct: 365 FDDLCLRVPSGKTVALVGGSGSGKSTVISL 394
>At3g15470.1 68416.m01962 WD-40 repeat family protein contains Pfam
PF00400: WD domain, G-beta repeat; similar to WD-repeat
protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
sapiens]
Length = 883
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 2/44 (4%)
Frame = +1
Query: 295 RETVQDTEDDGGCSSCY--RKSSCTHFGCWRRNSYF*SAGSSCS 420
R+ + +E GGC+SC KS T C R+ + S G+ CS
Sbjct: 145 RDHGECSESVGGCASCIVRSKSDITTSQCGDRDRRYTSPGNPCS 188
>At1g18750.1 68414.m02338 MADS-box protein (AGL65) similar to
homeodomain transcription factor (AGL30) GI:3461830 from
[Arabidopsis thaliana]; contains Pfam domain PF00319:
SRF-type transcription factor (DNA-binding and
dimerisation domain); PMID: 12837945
Length = 389
Score = 28.3 bits (60), Expect = 3.3
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = -1
Query: 473 LPHEHFADLVPVLSSCQSEHEEPADQ 396
LPH +PV SSC E +P DQ
Sbjct: 228 LPHREMDGSIPVYSSCFFESTKPEDQ 253
>At5g46840.1 68418.m05771 RNA recognition motif (RRM)-containing
protein contains InterPro entry IPR000504: RNA-binding
region RNP-1 (RNA recognition motif) (RRM)
Length = 501
Score = 27.9 bits (59), Expect = 4.4
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +2
Query: 398 DQLALRAPTGKKTVLVQGQRNAREADASLWPCSRSSA 508
D++ PTGK + QG R ++ D P +SSA
Sbjct: 388 DKVVTPTPTGKANLSYQGVRASKSGDDKKTPYQKSSA 424
>At4g35730.1 68417.m05071 expressed protein contains Pfam profile:
PF03398 eukaryotic protein of unknown function, DUF292
Length = 430
Score = 27.5 bits (58), Expect = 5.8
Identities = 16/44 (36%), Positives = 24/44 (54%)
Frame = +1
Query: 322 DGGCSSCYRKSSCTHFGCWRRNSYF*SAGSSCSDWQEDSTGTRS 453
D SS Y K + G RR+SY + G + SD++E+ T T +
Sbjct: 289 DSETSSYYAKPGAENRGMGRRHSYN-NPGINESDYEEEYTNTEA 331
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 27.5 bits (58), Expect = 5.8
Identities = 8/17 (47%), Positives = 10/17 (58%)
Frame = +1
Query: 331 CSSCYRKSSCTHFGCWR 381
CSSC+ K C CW+
Sbjct: 359 CSSCFGKPKCPKCSCWK 375
>At3g28380.1 68416.m03546 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1240
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +2
Query: 383 EILTFDQLALRAPTGKKTVLVQGQRNAREADASL 484
E FD L L+ P GK LV G + + SL
Sbjct: 373 ETTIFDDLCLKIPAGKTVALVGGSGSGKSTVISL 406
>At3g28345.1 68416.m03541 ABC transporter family protein similar to
P-glycoprotein [Arabidopsis thaliana] GI:3849833;
contains Pfam profiles PF00005: ABC transporter,
PF00664: ABC transporter transmembrane region
Length = 1240
Score = 27.1 bits (57), Expect = 7.7
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = +2
Query: 383 EILTFDQLALRAPTGKKTVLVQGQRNAREADASL 484
E FD LR P+GK LV G + + SL
Sbjct: 373 ETSIFDDFCLRVPSGKTVALVGGSGSGKSTVISL 406
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,140,845
Number of Sequences: 28952
Number of extensions: 260244
Number of successful extensions: 754
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 730
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 749
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 967280384
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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