Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00123
(832 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g30980.1 68415.m03778 shaggy-related protein kinase delta / A... 37 0.019
At1g06390.2 68414.m00676 shaggy-related protein kinase iota / AS... 34 0.13
At1g06390.1 68414.m00675 shaggy-related protein kinase iota / AS... 34 0.13
At1g22015.1 68414.m02754 galactosyltransferase family protein co... 30 2.2
At4g27370.1 68417.m03929 myosin family protein contains Pfam pro... 29 2.9
At1g57870.1 68414.m06566 shaggy-related protein kinase kappa, pu... 29 2.9
At5g26751.1 68418.m03187 shaggy-related protein kinase alpha / A... 29 5.0
At3g05840.2 68416.m00656 shaggy-related protein kinase gamma / A... 28 6.6
At3g05840.1 68416.m00655 shaggy-related protein kinase gamma / A... 28 6.6
At5g14640.1 68418.m01715 protein kinase family protein similar t... 28 8.7
At5g05080.1 68418.m00539 ubiquitin-conjugating enzyme, putative ... 28 8.7
At1g03000.1 68414.m00271 AAA-type ATPase family protein contains... 28 8.7
>At2g30980.1 68415.m03778 shaggy-related protein kinase delta /
ASK-delta / ASK-dzeta (ASK4) identical to shaggy-related
protein kinase delta (ASK-delta) (ASK- dzeta)
[Arabidopsis thaliana] SWISS-PROT:Q39010
Length = 412
Score = 36.7 bits (81), Expect = 0.019
Identities = 21/87 (24%), Positives = 42/87 (48%), Gaps = 3/87 (3%)
Frame = -3
Query: 485 GIYHLKLAKSYCHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYI 315
G+ ++ A CH ++P + + L H+ +L + K+L++G +I ++ Y
Sbjct: 183 GLAYIHTAPGVCHRDVKPQNLLVDPLTHQCKLCDFGSAKVLVKGEANISYICSRYYRAPE 242
Query: 314 LINPALQGRQSLEGYYCSCITAAAL*G 234
LI A + S++ + C+ A L G
Sbjct: 243 LIFGATEYTSSIDIWSAGCVLAELLLG 269
>At1g06390.2 68414.m00676 shaggy-related protein kinase iota /
ASK-iota (ASK9) (GSK1) identical to shaggy-related
protein kinase iota (ASK-iota) [Arabidopsis thaliana]
SWISS-PROT:Q39012
Length = 407
Score = 33.9 bits (74), Expect = 0.13
Identities = 20/87 (22%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = -3
Query: 485 GIYHLKLAKSYCHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYI 315
G+ ++ CH ++P + + L H+ +L + K+L++G +I ++ Y
Sbjct: 181 GLAYIHTVPGVCHRDVKPQNLLVDPLTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPE 240
Query: 314 LINPALQGRQSLEGYYCSCITAAAL*G 234
LI A + S++ + C+ A L G
Sbjct: 241 LIFGATEYTASIDIWSAGCVLAELLLG 267
>At1g06390.1 68414.m00675 shaggy-related protein kinase iota /
ASK-iota (ASK9) (GSK1) identical to shaggy-related
protein kinase iota (ASK-iota) [Arabidopsis thaliana]
SWISS-PROT:Q39012
Length = 407
Score = 33.9 bits (74), Expect = 0.13
Identities = 20/87 (22%), Positives = 41/87 (47%), Gaps = 3/87 (3%)
Frame = -3
Query: 485 GIYHLKLAKSYCHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYI 315
G+ ++ CH ++P + + L H+ +L + K+L++G +I ++ Y
Sbjct: 181 GLAYIHTVPGVCHRDVKPQNLLVDPLTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPE 240
Query: 314 LINPALQGRQSLEGYYCSCITAAAL*G 234
LI A + S++ + C+ A L G
Sbjct: 241 LIFGATEYTASIDIWSAGCVLAELLLG 267
>At1g22015.1 68414.m02754 galactosyltransferase family protein
contains Pfam profile: PF01762 galactosyltransferase
Length = 398
Score = 29.9 bits (64), Expect = 2.2
Identities = 20/63 (31%), Positives = 30/63 (47%), Gaps = 2/63 (3%)
Frame = -2
Query: 378 NINTRQNTVSSHKSKTLLYIYTNKSG--IARKTKPRRLLLFLYNGRRTVGSCAHIASVVY 205
N+ T +T++SH+SK +YI KSG + +KT R F G H +Y
Sbjct: 237 NLGTLASTLASHRSKPRVYIGCMKSGPVLTKKTAKYREPEFWKFGEEGNKYFRHATGQIY 296
Query: 204 FLS 196
+S
Sbjct: 297 AIS 299
>At4g27370.1 68417.m03929 myosin family protein contains Pfam
profiles: PF00063 myosin head (motor domain), PF00612 IQ
calmodulin-binding motif
Length = 1126
Score = 29.5 bits (63), Expect = 2.9
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +1
Query: 403 WYNSIIYTPFGRMCSWQ*DLANFKWYIPKVNKNSS 507
W NS Y ++C W AN +W++ K++ SS
Sbjct: 101 WINSAEYFVREKLCVWCRVAANGQWHLGKIHSTSS 135
>At1g57870.1 68414.m06566 shaggy-related protein kinase kappa,
putative / ASK-kappa, putative similar to shaggy-related
protein kinase kappa SP:Q39019 GI:717180 from
[Arabidopsis thaliana]
Length = 420
Score = 29.5 bits (63), Expect = 2.9
Identities = 21/108 (19%), Positives = 50/108 (46%), Gaps = 5/108 (4%)
Frame = -3
Query: 542 SRIKRFSTINLHELFLFTL--GIYHLKLAKSYCHEHMRPNGVYIMELYHRRELVE---NK 378
SR+ + + +L+ + + G+ +L CH ++P + + H+ ++ + K
Sbjct: 172 SRMNQLMPLIYVKLYTYQICRGLAYLHNCCGLCHRDIKPQNLLVNPHTHQLKICDFGSAK 231
Query: 377 ILIRGRIQSRHIRAKRYYTYILINPALQGRQSLEGYYCSCITAAAL*G 234
+L++G +I ++ Y LI A + +++ + C+ A L G
Sbjct: 232 VLVKGEPNISYICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLG 279
>At5g26751.1 68418.m03187 shaggy-related protein kinase alpha /
ASK-alpha (ASK1) identical to shaggy-related protein
kinase alpha SP:P43288 GI:460832 from [Arabidopsis
thaliana]
Length = 405
Score = 28.7 bits (61), Expect = 5.0
Identities = 25/112 (22%), Positives = 50/112 (44%), Gaps = 11/112 (9%)
Frame = -3
Query: 536 IKRFSTINLHELFLFT-LGIYHLKLAKSY-------CHEHMRPNGVYIMELYHRRELVE- 384
IK ++ +N ++ L Y + A SY CH ++P + + H+ +L +
Sbjct: 155 IKHYNKLNQRMPLIYVKLYTYQIFRALSYIHRCIGVCHRDIKPQNLLVNPHTHQVKLCDF 214
Query: 383 --NKILIRGRIQSRHIRAKRYYTYILINPALQGRQSLEGYYCSCITAAAL*G 234
K+L++G +I ++ Y LI A + +++ + C+ A L G
Sbjct: 215 GSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTAIDVWSAGCVLAELLLG 266
>At3g05840.2 68416.m00656 shaggy-related protein kinase gamma /
ASK-gamma (ASK3) identical to shaggy-related protein
kinase gamma SP:P43289 GI:456509 from [Arabidopsis
thaliana]
Length = 409
Score = 28.3 bits (60), Expect = 6.6
Identities = 17/76 (22%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = -3
Query: 452 CHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYILINPALQGRQS 282
CH ++P + + H+ +L + K+L++G +I ++ Y LI A + +
Sbjct: 195 CHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTA 254
Query: 281 LEGYYCSCITAAAL*G 234
++ + C+ A L G
Sbjct: 255 IDVWSAGCVLAELLLG 270
>At3g05840.1 68416.m00655 shaggy-related protein kinase gamma /
ASK-gamma (ASK3) identical to shaggy-related protein
kinase gamma SP:P43289 GI:456509 from [Arabidopsis
thaliana]
Length = 409
Score = 28.3 bits (60), Expect = 6.6
Identities = 17/76 (22%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = -3
Query: 452 CHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYILINPALQGRQS 282
CH ++P + + H+ +L + K+L++G +I ++ Y LI A + +
Sbjct: 195 CHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTA 254
Query: 281 LEGYYCSCITAAAL*G 234
++ + C+ A L G
Sbjct: 255 IDVWSAGCVLAELLLG 270
>At5g14640.1 68418.m01715 protein kinase family protein similar to
glycogen synthase kinase-3 homolog MsK-3 SP:P51139 from
[Medicago sativa]; contains Pfam profile PF00069:
Protein kinase domain
Length = 410
Score = 27.9 bits (59), Expect = 8.7
Identities = 17/76 (22%), Positives = 36/76 (47%), Gaps = 3/76 (3%)
Frame = -3
Query: 452 CHEHMRPNGVYIMELYHRRELVE---NKILIRGRIQSRHIRAKRYYTYILINPALQGRQS 282
CH ++P + + H+ +L + K+L++G +I ++ Y LI A + +
Sbjct: 196 CHRDIKPQNLLVNPHTHQVKLCDFGSAKVLVKGEPNISYICSRYYRAPELIFGATEYTTT 255
Query: 281 LEGYYCSCITAAAL*G 234
++ + C+ A L G
Sbjct: 256 IDIWSAGCVLAELLLG 271
>At5g05080.1 68418.m00539 ubiquitin-conjugating enzyme, putative
similar to SP|Q16763 Ubiquitin-conjugating enzyme E2-24
kDa (EC 6.3.2.19) (Ubiquitin- protein ligase) (Ubiquitin
carrier protein) {Homo sapiens}; contains Pfam profile
PF00179: Ubiquitin-conjugating enzyme
Length = 251
Score = 27.9 bits (59), Expect = 8.7
Identities = 9/28 (32%), Positives = 18/28 (64%)
Frame = -3
Query: 485 GIYHLKLAKSYCHEHMRPNGVYIMELYH 402
G++ +KLA S+ H P G ++ +++H
Sbjct: 57 GLFRMKLALSHDFPHSPPKGYFMTKIFH 84
>At1g03000.1 68414.m00271 AAA-type ATPase family protein contains
Pfam domain, PF00004: ATPase, AAA family ('A'TPases
'A'ssociated with diverse cellular 'A'ctivities)
Length = 941
Score = 27.9 bits (59), Expect = 8.7
Identities = 19/50 (38%), Positives = 25/50 (50%)
Frame = +2
Query: 302 PDLLVYMYNSVLLLCDETVFCLVLIFYFLPTLCDGIIPLYTLRLAACVHG 451
PDLLVY + L +ETV L + P LC + LR+A +HG
Sbjct: 338 PDLLVYRSKVPMPLQEETVNILASV--LSPPLCPSAL-ASKLRVAVLLHG 384
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,430,374
Number of Sequences: 28952
Number of extensions: 360824
Number of successful extensions: 805
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 778
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 805
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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