BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00113 (750 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 138 6e-34 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 135 5e-33 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 123 3e-29 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 123 3e-29 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 115 8e-27 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 62 6e-11 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 30 0.31 SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 29 0.94 SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.94 SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.6 SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pom... 28 1.6 SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharom... 28 1.6 SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 3.8 SPAC6F6.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.6 SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 6.6 SPBC776.10c |cog6||Golgi transport complex peripheral subunit Co... 26 6.6 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 138 bits (335), Expect = 6e-34 Identities = 67/80 (83%), Positives = 72/80 (90%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSI 689 FNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+ Sbjct: 148 FNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSL 207 Query: 690 LTIEDGIFEVKSTAGGHPLG 749 LTIE+GIFEVK+TAG LG Sbjct: 208 LTIEEGIFEVKATAGDTHLG 227 Score = 130 bits (315), Expect = 2e-31 Identities = 60/86 (69%), Positives = 69/86 (80%) Frame = +1 Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSM 429 PHNTIFDAKRLIGRKF+D VQ+DMKHWPF+ KP ++V YKGE KT PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSM 120 Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507 VL KM+ETAEAYLG V +AV+TVPA Sbjct: 121 VLMKMRETAEAYLGGKVTDAVVTVPA 146 Score = 112 bits (269), Expect = 6e-26 Identities = 51/58 (87%), Positives = 55/58 (94%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 135 bits (327), Expect = 5e-33 Identities = 66/80 (82%), Positives = 71/80 (88%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSI 689 FNDSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+ Sbjct: 148 FNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSL 207 Query: 690 LTIEDGIFEVKSTAGGHPLG 749 LTIE+GIFEVK+TAG LG Sbjct: 208 LTIEEGIFEVKATAGDTHLG 227 Score = 124 bits (300), Expect = 1e-29 Identities = 57/86 (66%), Positives = 68/86 (79%) Frame = +1 Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSM 429 PHNTIFDAKRLIGR+F D VQ+DMKHWPF+ KP I+V +KGE KT PEE+SSM Sbjct: 61 PHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSM 120 Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507 VL KM+E+AEA+LG V +AV+TVPA Sbjct: 121 VLLKMRESAEAFLGGKVTDAVVTVPA 146 Score = 112 bits (269), Expect = 6e-26 Identities = 51/58 (87%), Positives = 55/58 (94%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 ++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 123 bits (296), Expect = 3e-29 Identities = 56/80 (70%), Positives = 73/80 (91%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSI 689 FND+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+ Sbjct: 181 FNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSL 239 Query: 690 LTIEDGIFEVKSTAGGHPLG 749 L+I++G+FEV +T+G LG Sbjct: 240 LSIDNGVFEVLATSGDTHLG 259 Score = 103 bits (246), Expect = 4e-23 Identities = 47/86 (54%), Positives = 63/86 (73%) Frame = +1 Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSM 429 P NTIFD KRLIGRKF++ T+ D+K +PF +P ++V G+ K PEE+S+M Sbjct: 94 PENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAM 153 Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507 +L+KMK+TAEAYLGK V +AV+TVPA Sbjct: 154 ILSKMKQTAEAYLGKPVTHAVVTVPA 179 Score = 98.3 bits (234), Expect = 1e-21 Identities = 43/57 (75%), Positives = 50/57 (87%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 +GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ NP Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNP 94 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 123 bits (296), Expect = 3e-29 Identities = 60/80 (75%), Positives = 67/80 (83%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSI 689 F+DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+ Sbjct: 152 FSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSL 211 Query: 690 LTIEDGIFEVKSTAGGHPLG 749 L I+ G+FEV +TAG LG Sbjct: 212 LKIQGGVFEVLATAGDTHLG 231 Score = 106 bits (255), Expect = 3e-24 Identities = 50/86 (58%), Positives = 61/86 (70%) Frame = +1 Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSM 429 P NT+FDAKRLIGR++ED Q D+KHWPF+ P I+V Y GE K +E+S+M Sbjct: 65 PRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAM 124 Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507 VLTKMKE +EA L K V+ AVITVPA Sbjct: 125 VLTKMKEISEAKLNKRVEKAVITVPA 150 Score = 100 bits (239), Expect = 3e-22 Identities = 45/58 (77%), Positives = 51/58 (87%) Frame = +2 Query: 80 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 253 A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ AMNP Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 115 bits (276), Expect = 8e-27 Identities = 55/80 (68%), Positives = 66/80 (82%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSI 689 FNDSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SI Sbjct: 194 FNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKK--NDAIVAVFDLGGGTFDISI 251 Query: 690 LTIEDGIFEVKSTAGGHPLG 749 L + +G+FEV+ST G LG Sbjct: 252 LELNNGVFEVRSTNGDTHLG 271 Score = 74.9 bits (176), Expect = 1e-14 Identities = 37/86 (43%), Positives = 51/86 (59%) Frame = +1 Query: 250 PHNTIFDAKRLIGRKFEDATVQADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSM 429 P NT F KRLIGR+F++ VQ D+K P++ + KT P ++ Sbjct: 110 PENTFFATKRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGF 166 Query: 430 VLTKMKETAEAYLGKTVQNAVITVPA 507 +L+KM+ETA YLGK V+NAV+TVPA Sbjct: 167 ILSKMRETASTYLGKDVKNAVVTVPA 192 Score = 73.3 bits (172), Expect = 3e-14 Identities = 34/66 (51%), Positives = 45/66 (68%), Gaps = 1/66 (1%) Frame = +2 Query: 71 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAM 247 K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q + Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108 Query: 248 NPTTQY 265 NP + Sbjct: 109 NPENTF 114 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 62.5 bits (145), Expect = 6e-11 Identities = 35/84 (41%), Positives = 46/84 (54%), Gaps = 4/84 (4%) Frame = +3 Query: 510 FNDSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTF 677 F D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + Sbjct: 152 FTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNY 211 Query: 678 DVSILTIEDGIFEVKSTAGGHPLG 749 VSI+ G F +KST LG Sbjct: 212 SVSIVEFSRGQFHIKSTVCDRNLG 235 Score = 51.2 bits (117), Expect = 2e-07 Identities = 21/61 (34%), Positives = 39/61 (63%) Frame = +2 Query: 68 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAM 247 ++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEAS 62 Query: 248 N 250 N Sbjct: 63 N 63 Score = 35.9 bits (79), Expect = 0.006 Identities = 24/85 (28%), Positives = 40/85 (47%), Gaps = 1/85 (1%) Frame = +1 Query: 256 NTIFDAKRLIGRKFEDATVQ-ADMKHWPFEXXXXXXKPKIKVAYKGEDKTIFPEEVSSMV 432 NT+ KRL GR ++D ++ + + KV Y E+ ++ + Sbjct: 66 NTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAY 125 Query: 433 LTKMKETAEAYLGKTVQNAVITVPA 507 TK+K AEA L +V + VI++PA Sbjct: 126 FTKIKAIAEAELIGSVSDVVISIPA 150 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 41.5 bits (93), Expect = 1e-04 Identities = 17/56 (30%), Positives = 31/56 (55%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMN 250 +GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ N Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVRN 82 Score = 28.3 bits (60), Expect = 1.2 Identities = 13/38 (34%), Positives = 22/38 (57%) Frame = +1 Query: 391 EDKTIFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVP 504 ++K + E S L ++ E+AE +LG V V++VP Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVP 171 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 30.3 bits (65), Expect = 0.31 Identities = 21/67 (31%), Positives = 31/67 (46%), Gaps = 1/67 (1%) Frame = +2 Query: 83 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPTT 259 + ID GT ++ + + G +EI+ R S VAF ER+ G A N P Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83 Query: 260 QYSMPNV 280 +S+ NV Sbjct: 84 -HSIRNV 89 >SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 2244 Score = 28.7 bits (61), Expect = 0.94 Identities = 15/45 (33%), Positives = 25/45 (55%) Frame = +2 Query: 179 PSYVAFTDTERLIGDAAKNQVAMNPTTQYSMPNVSSDVSSKMLLC 313 P +A T ER++ AAK A+N T Y++ ++ D K++ C Sbjct: 1252 PQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIEC 1296 >SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosaccharomyces pombe|chr 1|||Manual Length = 378 Score = 28.7 bits (61), Expect = 0.94 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = +3 Query: 75 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 170 HP S W+P LVS+ W +SP+++ T Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314 >SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 574 Score = 27.9 bits (59), Expect = 1.6 Identities = 16/44 (36%), Positives = 17/44 (38%) Frame = -3 Query: 748 PSGCPPAVDFTSKIPSSMVRMDTSKVPPPRSKISTFRSPVPFLS 617 P PP + IP PPPRS ST R P P S Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSS 381 >SPAC212.11 |tlh1||RecQ type DNA helicase|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 1887 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -2 Query: 551 GTCIFCGLSLRVIEDAGTVITAFCTVLPR*ASAVSFIF 438 G C+FCGL+ D+ T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 >SPBCPT2R1.08c |tlh2||RecQ type DNA helicase Tlh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1919 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/38 (31%), Positives = 20/38 (52%) Frame = -2 Query: 551 GTCIFCGLSLRVIEDAGTVITAFCTVLPR*ASAVSFIF 438 G C+FCGL+ D+ T+ C R A+ +S ++ Sbjct: 1827 GLCLFCGLTKFEHADSDMAYTSDCRSWARKANLISLVY 1864 >SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 26.6 bits (56), Expect = 3.8 Identities = 18/53 (33%), Positives = 27/53 (50%) Frame = +3 Query: 108 TLALVSSSTGRWRSSPTTRATGPLRLMLRSQTPSVSSEMPPRTRWR*TPQHNI 266 T +L S+STG+ T++ T L + PS S+ P T + TP +NI Sbjct: 282 TSSLTSNSTGK-----TSKTTDLLIAASKKSLPSNSTPSKPNTSFFETPHNNI 329 >SPAC6F6.16c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 282 Score = 25.8 bits (54), Expect = 6.6 Identities = 9/25 (36%), Positives = 16/25 (64%) Frame = -3 Query: 736 PPAVDFTSKIPSSMVRMDTSKVPPP 662 PPA++FTS I + + ++ + P P Sbjct: 187 PPAINFTSDICNEQIELEYKRKPIP 211 >SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosaccharomyces pombe|chr 1|||Manual Length = 411 Score = 25.8 bits (54), Expect = 6.6 Identities = 16/53 (30%), Positives = 26/53 (49%) Frame = -2 Query: 161 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 3 G W SP S + H ++++ +F +RVL C+ L K +K+A L Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323 >SPBC776.10c |cog6||Golgi transport complex peripheral subunit Cog6 |Schizosaccharomyces pombe|chr 2|||Manual Length = 675 Score = 25.8 bits (54), Expect = 6.6 Identities = 10/28 (35%), Positives = 16/28 (57%) Frame = +2 Query: 224 AAKNQVAMNPTTQYSMPNVSSDVSSKML 307 A K Q+ +NP + +PNV D + +L Sbjct: 323 ARKRQIQLNPFPPWDVPNVLEDQMNSLL 350 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,306,278 Number of Sequences: 5004 Number of extensions: 70874 Number of successful extensions: 259 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 226 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 253 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 357280532 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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