BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00111 (720 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) riboso... 143 1e-34 At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) 143 1e-34 At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contai... 143 1e-34 At4g10710.1 68417.m01751 transcriptional regulator-related simil... 31 0.77 At1g29760.1 68414.m03639 expressed protein 31 0.77 At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putativ... 29 2.3 At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, ... 29 3.1 At1g14210.1 68414.m01682 ribonuclease T2 family protein contains... 28 5.4 At5g63000.1 68418.m07904 expressed protein 28 7.2 At3g56260.1 68416.m06252 expressed protein 28 7.2 At4g08180.3 68417.m01353 oxysterol-binding family protein simila... 27 9.5 At4g08180.2 68417.m01352 oxysterol-binding family protein simila... 27 9.5 At4g08180.1 68417.m01351 oxysterol-binding family protein simila... 27 9.5 >At5g58420.1 68418.m07315 40S ribosomal protein S4 (RPS4D) ribosomal protein S4, Arabidopsis thaliana, PIR:T48480 Length = 262 Score = 143 bits (346), Expect = 1e-34 Identities = 64/84 (76%), Positives = 73/84 (86%) Frame = +2 Query: 2 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 181 RG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ Sbjct: 3 RGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVI 62 Query: 182 KIVKQRLIKVDGKVRTDPTYPAGY 253 I+ QR I+VDGKVRTD TYPAG+ Sbjct: 63 SILMQRHIQVDGKVRTDKTYPAGF 86 Score = 123 bits (296), Expect = 1e-28 Identities = 56/94 (59%), Positives = 69/94 (73%) Frame = +1 Query: 256 DVVSIEKTNELYRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 435 DVVSI KTNE +RL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147 Query: 436 RYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV 537 RYPDPLIK ND+I+LD+ KI++ G V Sbjct: 148 RYPDPLIKPNDTIKLDLEANKIVEFIKFDVGNVV 181 Score = 91.5 bits (217), Expect = 5e-19 Identities = 39/61 (63%), Positives = 49/61 (80%) Frame = +3 Query: 510 IKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKAR 689 IKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGT 232 Query: 690 R 692 + Sbjct: 233 K 233 >At5g07090.1 68418.m00804 40S ribosomal protein S4 (RPS4B) Length = 262 Score = 143 bits (346), Expect = 1e-34 Identities = 64/84 (76%), Positives = 73/84 (86%) Frame = +2 Query: 2 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 181 RG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ Sbjct: 3 RGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVI 62 Query: 182 KIVKQRLIKVDGKVRTDPTYPAGY 253 I+ QR I+VDGKVRTD TYPAG+ Sbjct: 63 SILMQRHIQVDGKVRTDKTYPAGF 86 Score = 122 bits (295), Expect = 2e-28 Identities = 56/94 (59%), Positives = 69/94 (73%) Frame = +1 Query: 256 DVVSIEKTNELYRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 435 DVVSI KTNE +RL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147 Query: 436 RYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV 537 RYPDPLIK ND+I+LD+ KI++ G V Sbjct: 148 RYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181 Score = 91.5 bits (217), Expect = 5e-19 Identities = 39/61 (63%), Positives = 49/61 (80%) Frame = +3 Query: 510 IKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKAR 689 IKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGT 232 Query: 690 R 692 + Sbjct: 233 K 233 >At2g17360.1 68415.m02005 40S ribosomal protein S4 (RPS4A) contains ribosomal protein S4 signature from residues 8 to 22 Length = 261 Score = 143 bits (346), Expect = 1e-34 Identities = 64/84 (76%), Positives = 73/84 (86%) Frame = +2 Query: 2 RGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNEVL 181 RG KKHLKRLNAPK WMLDKLGG +AP+PS+GPHK RECLPLV+ +RNRLKYALT EV+ Sbjct: 3 RGLKKHLKRLNAPKHWMLDKLGGAFAPKPSSGPHKSRECLPLVLIIRNRLKYALTYREVI 62 Query: 182 KIVKQRLIKVDGKVRTDPTYPAGY 253 I+ QR I+VDGKVRTD TYPAG+ Sbjct: 63 SILMQRHIQVDGKVRTDKTYPAGF 86 Score = 122 bits (295), Expect = 2e-28 Identities = 56/94 (59%), Positives = 69/94 (73%) Frame = +1 Query: 256 DVVSIEKTNELYRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYLVTHDGRTI 435 DVVSI KTNE +RL+YD KGRF +H I EEAK+KLCKV+ + G K +PYL T+DGRTI Sbjct: 88 DVVSIPKTNENFRLLYDTKGRFRLHSIKDEEAKFKLCKVRSIQFGQKGIPYLNTYDGRTI 147 Query: 436 RYPDPLIKVNDSIQLDIATTKIMDSSSLSPGTCV 537 RYPDPLIK ND+I+LD+ KI++ G V Sbjct: 148 RYPDPLIKPNDTIKLDLEENKIVEFIKFDVGNVV 181 Score = 91.5 bits (217), Expect = 5e-19 Identities = 39/61 (63%), Positives = 49/61 (80%) Frame = +3 Query: 510 IKFESGNLCMITGGRNLGRVGTIVSRERHPGSFDIVHIKDSTGHTFATRLNNVFIIGKAR 689 IKF+ GN+ M+TGGRN GRVG I +RE+H GSF+ +HI+DSTGH FATRL NV+ IGK Sbjct: 173 IKFDVGNVVMVTGGRNRGRVGVIKNREKHKGSFETIHIQDSTGHEFATRLGNVYTIGKGT 232 Query: 690 R 692 + Sbjct: 233 K 233 >At4g10710.1 68417.m01751 transcriptional regulator-related similar to chromatin-specific transcription elongation factor FACT 140 kDa subunit (GI:5499741) [Homo sapiens] Length = 1074 Score = 31.1 bits (67), Expect = 0.77 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = -1 Query: 120 KHSRNLWGPVDGLGAYTPPSLSNIHAL 40 KHS +LWG D L TPP+ ++ L Sbjct: 44 KHSADLWGSADALAIATPPASDDLRYL 70 >At1g29760.1 68414.m03639 expressed protein Length = 526 Score = 31.1 bits (67), Expect = 0.77 Identities = 14/31 (45%), Positives = 17/31 (54%) Frame = -1 Query: 231 SVLTFPSTFMRRCFTIFRTSFPVKAYFRRFL 139 S+LTFP +R CF F F + RRFL Sbjct: 195 SLLTFPPWLLRNCFLFFFDPFSTIRFGRRFL 225 >At4g38880.1 68417.m05506 amidophosphoribosyltransferase, putative / glutamine phosphoribosylpyrophosphate amidotransferase, putative / phosphoribosyldiphosphate 5-amidotransferase, putative similar to amidophosphoribosyltransferase [Arabidopsis thaliana] GI:469195; contains Pfam profiles PF00156: Phosphoribosyl transferase domain, PF00310: Glutamine amidotransferases class-II Length = 532 Score = 29.5 bits (63), Expect = 2.3 Identities = 28/91 (30%), Positives = 40/91 (43%) Frame = +3 Query: 396 ECSVLGNA*WPHHPLSRPTYQSQRFHPVRHCNYEDYGLIKFESGNLCMITGGRNLGRVGT 575 EC V+G P SR +Y + H ++H E G++ L ITG + V T Sbjct: 76 ECGVVGIHGDPE--ASRLSYLA--LHALQHRGQEGAGIVAANQNGLESITGVGLVSDVFT 131 Query: 576 IVSRERHPGSFDIVHIKDSTGHTFATRLNNV 668 PG I H++ ST + A+ L NV Sbjct: 132 ESKLNNLPGDIAIGHVRYST--SGASMLKNV 160 >At2g38050.1 68415.m04671 3-oxo-5-alpha-steroid 4-dehydrogenase, putative / steroid 5-alpha-reductase, putative identical to gi:1280611; contains a steroid 5-alpha reductase, C-terminal domain Length = 262 Score = 29.1 bits (62), Expect = 3.1 Identities = 15/39 (38%), Positives = 19/39 (48%) Frame = -1 Query: 210 TFMRRCFTIFRTSFPVKAYFRRFLRKITRGKHSRNLWGP 94 TF R C + P A +FL+ GKH+R WGP Sbjct: 8 TFFRYCLLTLIFAGPPTAVLLKFLQA-PYGKHNRTGWGP 45 >At1g14210.1 68414.m01682 ribonuclease T2 family protein contains similarity to RNase GI:7768564 from [Nicotiana tabacum]; contains Pfam profile PF00445: Ribonuclease T2 family Length = 247 Score = 28.3 bits (60), Expect = 5.4 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = +1 Query: 493 TKIMDSSSLSPGTCV*SREAVTWGVWAPSCPARD 594 T + D S +S C ++ WGVWA CP+ + Sbjct: 79 TNLFDISKISDLVCQMEKKWTEWGVWA--CPSNE 110 >At5g63000.1 68418.m07904 expressed protein Length = 201 Score = 27.9 bits (59), Expect = 7.2 Identities = 18/48 (37%), Positives = 23/48 (47%) Frame = -2 Query: 638 SRGVLDVHNVEGAGMSLAGHDGAHTPQVTASRDHTQVPGLKLDESIIF 495 +RGV DV NV GAG + A G P A R + G L ++ F Sbjct: 110 TRGVHDVFNVVGAGSATAAVFGLIMPGSLAWRARNVLLGSVLGATVCF 157 >At3g56260.1 68416.m06252 expressed protein Length = 176 Score = 27.9 bits (59), Expect = 7.2 Identities = 21/73 (28%), Positives = 35/73 (47%), Gaps = 2/73 (2%) Frame = +3 Query: 426 PHHPLSRPTYQSQRFHPVRHCNYEDYGLIKFESGNLCMITGGRNLGRVGTI--VSRERHP 599 P H L + ++ P+ + NYE+ L + LC GGR LG ++ V+R Sbjct: 95 PPHSLKKRMSHDKK-SPLLYANYEEDELRSSPTSTLCCSNGGR-LGCSSSMGSVTRALFS 152 Query: 600 GSFDIVHIKDSTG 638 GS + + ++TG Sbjct: 153 GSMEGMKRHETTG 165 >At4g08180.3 68417.m01353 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 27.5 bits (58), Expect = 9.5 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 143 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 241 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735 >At4g08180.2 68417.m01352 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 813 Score = 27.5 bits (58), Expect = 9.5 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 143 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 241 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 703 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 735 >At4g08180.1 68417.m01351 oxysterol-binding family protein similar to SP|Q969R2 Oxysterol-binding protein 2 {Homo sapiens}; contains Pfam profiles PF00169: PH domain, PF01237: Oxysterol-binding protein Length = 814 Score = 27.5 bits (58), Expect = 9.5 Identities = 12/33 (36%), Positives = 20/33 (60%) Frame = +2 Query: 143 NRLKYALTGNEVLKIVKQRLIKVDGKVRTDPTY 241 N ++A+T NE+ +K+RL D ++R D Y Sbjct: 704 NLTRFAITLNELTPGLKERLPPTDSRLRPDQRY 736 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,659,899 Number of Sequences: 28952 Number of extensions: 401023 Number of successful extensions: 1191 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 1124 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1187 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1565336320 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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