BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00108 (594 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB17E12.13 |rpl1802|rpl18-2|60S ribosomal protein L18|Schizos... 108 7e-25 SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S... 108 7e-25 SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces po... 29 0.51 SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|c... 28 0.89 SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe... 28 1.2 SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr ... 28 1.2 SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces ... 27 1.6 SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase... 27 2.1 SPAC14C4.10c |||Nudix family hydrolase|Schizosaccharomyces pombe... 27 2.1 SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces... 27 2.1 SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces ... 26 3.6 SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|... 26 4.8 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 26 4.8 SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosacchar... 25 6.3 SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subuni... 25 8.3 SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces p... 25 8.3 >SPAPB17E12.13 |rpl1802|rpl18-2|60S ribosomal protein L18|Schizosaccharomyces pombe|chr 1|||Manual Length = 187 Score = 108 bits (259), Expect = 7e-25 Identities = 53/90 (58%), Positives = 67/90 (74%) Frame = +3 Query: 243 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 422 G +D RL +PK++VAAL T+ ARARIL AGGE+LT DQLALRAPTG TVL++G++ Sbjct: 86 GTVTDDERLLTVPKLSVAALRFTKSARARILKAGGEVLTLDQLALRAPTGSNTVLLRGKK 145 Query: 423 NAREAVRHFGPAPGAPRSHTKPYVRTKGQK 512 +AREA RHFG P H PYVR++G+K Sbjct: 146 HAREAYRHFG---FGPHKHKAPYVRSEGRK 172 Score = 63.7 bits (148), Expect = 2e-11 Identities = 33/87 (37%), Positives = 50/87 (57%), Gaps = 2/87 (2%) Frame = +1 Query: 1 IDINHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPIS 180 IDI H RK +R++ S+++ T+++FN+ +L+RLF S+ NRPPIS Sbjct: 3 IDIERHHVRKSQRSKPASENVYLKLLVKLYRFLARRTDSRFNKAILKRLFQSKTNRPPIS 62 Query: 181 VSRLA--RHMKKPPREGLIAVVVGTVT 255 +S++A K EG V+VGTVT Sbjct: 63 ISKIAALTSRKSASLEGKTTVIVGTVT 89 >SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|Schizosaccharomyces pombe|chr 2|||Manual Length = 187 Score = 108 bits (259), Expect = 7e-25 Identities = 52/90 (57%), Positives = 67/90 (74%) Frame = +3 Query: 243 GDSHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQR 422 G +D R+ +PK+++AAL T+ ARARIL AGGE+LT DQLALRAPTG TVLV+G++ Sbjct: 86 GTVTDDERMLTVPKLSIAALRFTKSARARILKAGGEVLTLDQLALRAPTGSNTVLVRGKK 145 Query: 423 NAREAVRHFGPAPGAPRSHTKPYVRTKGQK 512 +AREA RHFG P H PYVR++G+K Sbjct: 146 HAREAYRHFG---FGPHKHKAPYVRSEGRK 172 Score = 59.7 bits (138), Expect = 3e-10 Identities = 31/87 (35%), Positives = 49/87 (56%), Gaps = 2/87 (2%) Frame = +1 Query: 1 IDINHKHDRKVRRTEVKSQDIXXXXXXXXXXXXXXXTNAKFNQIVLRRLFMSRINRPPIS 180 IDI H +K +R++ S+++ T+++FN+ +L+RLF S+ NRPPIS Sbjct: 3 IDIERHHVKKSQRSKPASENVYLKLLVKLYRFLARRTDSRFNKAILKRLFQSKTNRPPIS 62 Query: 181 VSRLA--RHMKKPPREGLIAVVVGTVT 255 +S++A K + VVVGTVT Sbjct: 63 ISKIAALTSRKSASSQNKTTVVVGTVT 89 >SPBC29A10.09c |||CAF1 family ribonuclease|Schizosaccharomyces pombe|chr 2|||Manual Length = 427 Score = 29.1 bits (62), Expect = 0.51 Identities = 15/39 (38%), Positives = 24/39 (61%) Frame = -2 Query: 356 KNFSSSSQNACTSFFGNMKSSHRHLRYLVQSHVIVTVPT 240 KN+ SS + TS + N+ +S+R +R +QS V + PT Sbjct: 170 KNWLSSELSHSTSKYLNITTSNRFIRKAIQSLVKIEFPT 208 >SPBC16A3.17c |||membrane transporter|Schizosaccharomyces pombe|chr 2|||Manual Length = 599 Score = 28.3 bits (60), Expect = 0.89 Identities = 24/73 (32%), Positives = 34/73 (46%), Gaps = 4/73 (5%) Frame = -2 Query: 518 LLFLSLGANIGFSVRARCSWSRAKVTHCLTSISLTLYQYCLLASRSTKSQLIKS----KN 351 LL +SLGA+ G W RA + C I L+ + + TK + KN Sbjct: 304 LLGISLGASSG-------KWRRANIL-CYLIIGGCLFVFAFIYDTFTKRNAVLPPPFFKN 355 Query: 350 FSSSSQNACTSFF 312 SS++ AC+SFF Sbjct: 356 RSSAALLACSSFF 368 >SPAC16A10.01 |||DUF1212 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 830 Score = 27.9 bits (59), Expect = 1.2 Identities = 17/55 (30%), Positives = 27/55 (49%) Frame = -2 Query: 515 LFLSLGANIGFSVRARCSWSRAKVTHCLTSISLTLYQYCLLASRSTKSQLIKSKN 351 LFLS G IG ++ C++ I+++ Y Y LL T S LI +++ Sbjct: 630 LFLSFGITIGSALYGWMDKDATDADTCMSIIAVSPYWYILLIPIFTLSLLIVTQS 684 >SPAC5D6.07c |||PXA domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 495 Score = 27.9 bits (59), Expect = 1.2 Identities = 12/38 (31%), Positives = 21/38 (55%) Frame = +3 Query: 258 DVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQL 371 D+ Y+IP + + + TE+A+ R G+IL D + Sbjct: 59 DLLFYEIPFLLIKHIENTEEAKLRFALPQGQILEIDTI 96 >SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 504 Score = 27.5 bits (58), Expect = 1.6 Identities = 12/25 (48%), Positives = 17/25 (68%) Frame = -2 Query: 359 SKNFSSSSQNACTSFFGNMKSSHRH 285 SKN SS N+ TSFF ++ + +RH Sbjct: 2 SKNSFSSMANSVTSFFQSLTTPNRH 26 >SPBC3B8.07c |dsd1|SDCB3B8.07c|dihydroceramide delta-4 desaturase|Schizosaccharomyces pombe|chr 2|||Manual Length = 362 Score = 27.1 bits (57), Expect = 2.1 Identities = 10/41 (24%), Positives = 22/41 (53%) Frame = -1 Query: 327 VHELFR*HEEQPPSSSVSCTVSRHCDCPHYHGNQTLTRWLL 205 VH+ + + E+P S + + H + +G + LT+W++ Sbjct: 26 VHQFYWTYTEEPHKSRRAAILKAHPEIASLNGYEPLTKWIV 66 >SPAC14C4.10c |||Nudix family hydrolase|Schizosaccharomyces pombe|chr 1|||Manual Length = 329 Score = 27.1 bits (57), Expect = 2.1 Identities = 14/51 (27%), Positives = 27/51 (52%), Gaps = 4/51 (7%) Frame = +1 Query: 337 LLEEKFLLLISWLFVLRLARRQY----WYKVSEMLVRQCVTLALLQEHRAL 477 LL F+ ++ ++ LRL + WY ++++L+ +C T + RAL Sbjct: 134 LLLSSFVFILPYMPSLRLQESEVFSAQWYPLADLLLPECQTRIQIDSSRAL 184 >SPBC15D4.10c |amo1||nuclear rim protein Amo1|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 27.1 bits (57), Expect = 2.1 Identities = 16/58 (27%), Positives = 30/58 (51%) Frame = -2 Query: 404 YCLLASRSTKSQLIKSKNFSSSSQNACTSFFGNMKSSHRHLRYLVQSHVIVTVPTTTA 231 Y + +ST + + S F+ +QN+ + F N +S + + Q++ I PTT+A Sbjct: 160 YKSIFDKSTSNSTVTSNQFNKPTQNSPFNSFSNNNNSFNNNQ---QANDIFGAPTTSA 214 >SPAC1A6.10 ||SPAC30D11.15c|Moeb/ThiF domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 485 Score = 26.2 bits (55), Expect = 3.6 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 3/48 (6%) Frame = +1 Query: 397 RQYWYKVSEMLVRQCVTLALLQ--EHRAL-TLNPMFAPRDRKSKAQSS 531 + +W VS + CV L L+ +HR++ L M P KS SS Sbjct: 30 KSFWIAVSSSVTTACVILGTLEFRKHRSIRRLQSMIVPEAGKSIQLSS 77 >SPCC1682.11c |||DUF580 family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 574 Score = 25.8 bits (54), Expect = 4.8 Identities = 12/51 (23%), Positives = 24/51 (47%) Frame = -2 Query: 305 MKSSHRHLRYLVQSHVIVTVPTTTAIKPSRGGFFMWRAKRDTEIGGRLIRL 153 +K+S HL +Q +++ + P +++WR + E +IRL Sbjct: 231 LKASRIHLETSIQPKLMLLTGIILLVAPILLSYYVWRRRIHFETSFNIIRL 281 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 25.8 bits (54), Expect = 4.8 Identities = 21/77 (27%), Positives = 33/77 (42%) Frame = -2 Query: 464 SWSRAKVTHCLTSISLTLYQYCLLASRSTKSQLIKSKNFSSSSQNACTSFFGNMKSSHRH 285 S S + + TS S + +S S+ S S + SSSS + +S + +SHR Sbjct: 122 SSSSSSLVSSTTSSSSSATPSTTSSSSSSSSSSSSSSSKSSSSSSKSSSRSSSRTTSHRT 181 Query: 284 LRYLVQSHVIVTVPTTT 234 + S+ P TT Sbjct: 182 TSHKSSSYRPTVFPYTT 198 >SPAC343.18 |rfp2||ubiquitin-protein ligase E3 Rfp2|Schizosaccharomyces pombe|chr 1|||Manual Length = 205 Score = 25.4 bits (53), Expect = 6.3 Identities = 17/62 (27%), Positives = 28/62 (45%) Frame = +3 Query: 249 SHNDVRLYKIPKMTVAALHVTEKARARILAAGGEILTFDQLALRAPTGKKTVLVQGQRNA 428 +H D L +P + VT R + GG + R+ T ++T+L G RN+ Sbjct: 58 NHLDANLSNVPTINSIPSPVT---RPPVAVGGGIFYGARRTRNRSQTQRRTLLENGFRNS 114 Query: 429 RE 434 R+ Sbjct: 115 RK 116 >SPCC297.06c ||SPCC737.01c|mitochondrial ribosomal protein subunit 8|Schizosaccharomyces pombe|chr 3|||Manual Length = 230 Score = 25.0 bits (52), Expect = 8.3 Identities = 9/32 (28%), Positives = 18/32 (56%) Frame = +3 Query: 363 DQLALRAPTGKKTVLVQGQRNAREAVRHFGPA 458 D++ + P G K + Q ++ R A++H+ A Sbjct: 120 DEILPKEPAGSKEAMQQKKKEKRAALKHYREA 151 >SPAC20G8.09c |||N-acetyltransferase Nat10 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1033 Score = 25.0 bits (52), Expect = 8.3 Identities = 12/30 (40%), Positives = 18/30 (60%) Frame = +1 Query: 397 RQYWYKVSEMLVRQCVTLALLQEHRALTLN 486 R +YK SE ++ Q + +LQ+ ALT N Sbjct: 104 RYCYYKESEKILGQTYGMLVLQDFEALTPN 133 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,417,976 Number of Sequences: 5004 Number of extensions: 49929 Number of successful extensions: 144 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 134 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 142 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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