BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= heS00096 (769 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 123 3e-29 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 31 0.14 SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 2.2 SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 27 3.0 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 5.2 SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Sch... 26 6.8 SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Sc... 26 6.8 SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 25 9.0 SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomy... 25 9.0 SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1||... 25 9.0 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 9.0 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 123 bits (296), Expect = 3e-29 Identities = 58/82 (70%), Positives = 68/82 (82%) Frame = +1 Query: 256 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAG 435 DP LD+ K+L+I+I P+K L+I DTGIGMTK DL+NNLG IAKSGTK FMEA +G Sbjct: 53 DPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAAASG 112 Query: 436 ADISMIGQFGVGFYSSYLVADR 501 ADISMIGQFGVGFYS+YLVAD+ Sbjct: 113 ADISMIGQFGVGFYSAYLVADK 134 Score = 85.8 bits (203), Expect = 6e-18 Identities = 41/50 (82%), Positives = 46/50 (92%) Frame = +2 Query: 104 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESL 253 + ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIRY+SL Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQSL 51 Score = 83.4 bits (197), Expect = 3e-17 Identities = 41/80 (51%), Positives = 54/80 (67%), Gaps = 1/80 (1%) Frame = +3 Query: 513 SKHNDDEQYVWESSAGGSFTVRPDSGEP-LGRGTKIVLHVKEDLAEFMXXXXXXXXXXXX 689 SKHNDDEQY+WESSAGGSFTV D+ P L RGT+I L +KED +++ Sbjct: 139 SKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVKKH 198 Query: 690 SQFIGYPIKLMVEKNAKKEL 749 S+FI YPI+L+V + +KE+ Sbjct: 199 SEFISYPIQLVVTREVEKEV 218 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 31.5 bits (68), Expect = 0.14 Identities = 23/66 (34%), Positives = 36/66 (54%), Gaps = 5/66 (7%) Frame = +2 Query: 176 SNKEIFLRELISNSSDALDKIRYESLRIRQNSI-VAKSCTSRSFPTRTRALLRSSI---- 340 S+ +L +L+++ D L K+R E LRIR+ I V S T TRA+++ + Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRP 384 Query: 341 PVLV*P 358 PVL+ P Sbjct: 385 PVLMVP 390 >SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.5 bits (58), Expect = 2.2 Identities = 20/53 (37%), Positives = 30/53 (56%) Frame = -3 Query: 413 KALVPDFAMVPKLFTKSALVIPIPVSMIVRVPSFLLGMILMYSSLPLSSFDGS 255 KA++P F + +AL+ PI ++ I +F+ IL + S PLS FDGS Sbjct: 486 KAVIPIFTNQVS-YINNALIRPI-IAYINSTRTFI--PILCHVSKPLSDFDGS 534 >SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 940 Score = 27.1 bits (57), Expect = 3.0 Identities = 12/40 (30%), Positives = 26/40 (65%), Gaps = 5/40 (12%) Frame = +2 Query: 191 FLRELISNSSDALDKIRYESLRI-----RQNSIVAKSCTS 295 F+R+++ +D L+K+R++SL + +S+V ++ TS Sbjct: 224 FIRKVVLTKADGLEKLRFQSLLVSLTATNNSSVVFEAATS 263 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 26.2 bits (55), Expect = 5.2 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = -2 Query: 390 DGSQIVHQIGLGHTNTGIDDRKSALVLVGNDLDVQL 283 DG V I G +DDR+ L+ + NDL+V L Sbjct: 2 DGKPQVEVIVNGQVVPNLDDREYRLIKLENDLEVLL 37 >SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 1|||Manual Length = 221 Score = 25.8 bits (54), Expect = 6.8 Identities = 14/55 (25%), Positives = 23/55 (41%) Frame = -2 Query: 360 LGHTNTGIDDRKSALVLVGNDLDVQLFATIEF*RILRDSYLILSKASDELEISSR 196 LG G+D+ + LV N+ + +E RI + YL+ D + R Sbjct: 36 LGRKRAGVDEFPLCIHLVSNEYEQITSEALEAARICANKYLVKIGGKDSFHLRVR 90 >SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 2|||Manual Length = 221 Score = 25.8 bits (54), Expect = 6.8 Identities = 14/55 (25%), Positives = 23/55 (41%) Frame = -2 Query: 360 LGHTNTGIDDRKSALVLVGNDLDVQLFATIEF*RILRDSYLILSKASDELEISSR 196 LG G+D+ + LV N+ + +E RI + YL+ D + R Sbjct: 36 LGRKRAGVDEFPLCIHLVSNEYEQITSEALEAARICANKYLVKIGGKDSFHLRVR 90 >SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 25.4 bits (53), Expect = 9.0 Identities = 8/29 (27%), Positives = 16/29 (55%) Frame = +3 Query: 294 QDHSQQERGHSYDHRYRYWYDQGRFGEQF 380 +DH++++ H+ +Y YD R E + Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIWENY 631 >SPAC26A3.17c ||SPAC8E11.11|N-methyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 357 Score = 25.4 bits (53), Expect = 9.0 Identities = 15/40 (37%), Positives = 19/40 (47%) Frame = +2 Query: 158 IINTFYSNKEIFLRELISNSSDALDKIRYESLRIRQNSIV 277 II+TF KE L +I D L +R R+N IV Sbjct: 198 IIDTFLQEKEPSLHVIIEPHPDVLKHMRKNGWMDRENVIV 237 >SPAC19D5.04 |ptr1||HECT domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 3227 Score = 25.4 bits (53), Expect = 9.0 Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%) Frame = +2 Query: 245 ESLRIRQNSIVAKSCTSRSFPTRTRALLR-SSIP 343 E+LR+ + I SC+SR+F + SSIP Sbjct: 2617 ENLRLAASLITTDSCSSRTFQNALSVMFHLSSIP 2650 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 25.4 bits (53), Expect = 9.0 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = +1 Query: 256 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLG 384 DPSK ++Y K+ + + L D+GI ++ L+ +G Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIG 2514 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,204,204 Number of Sequences: 5004 Number of extensions: 69988 Number of successful extensions: 198 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 192 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 197 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 369323696 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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