Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= heS00089
(731 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g39410.1 68418.m04774 expressed protein 93 2e-19
At3g18890.1 68416.m02399 expressed protein similar to UV-B and o... 35 0.064
At1g22380.1 68414.m02799 UDP-glucoronosyl/UDP-glucosyl transfera... 33 0.20
At4g02570.1 68417.m00351 cullin family protein similar to cullin... 30 1.8
At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transfera... 29 2.4
At3g53540.1 68416.m05912 expressed protein 29 3.2
At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S ri... 29 4.2
At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family... 29 4.2
At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase ... 28 5.6
At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase ... 28 5.6
At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 28 7.3
At3g22420.1 68416.m02829 protein kinase family protein contains ... 28 7.3
At1g08540.1 68414.m00946 RNA polymerase sigma subunit SigB (sigB... 28 7.3
At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 / cy... 27 9.7
>At5g39410.1 68418.m04774 expressed protein
Length = 454
Score = 92.7 bits (220), Expect = 2e-19
Identities = 39/85 (45%), Positives = 62/85 (72%), Gaps = 2/85 (2%)
Frame = +2
Query: 260 GPYRLYGEQVVKAAIESKTHYVDVSGEPEFMELMQLNYNEQAKDAGVYVISACGFDSIPC 439
GP+R++G+ VV A +S Y+D+SGEPEFME M+ NY+++A++ G ++SACGFDSIP
Sbjct: 100 GPFRIHGDPVVSACADSGCDYLDISGEPEFMERMEANYHDRAEETGSLIVSACGFDSIPA 159
Query: 440 DLGVIHLMKNF--EGVLNSVESYIT 508
+LG++ K + V N +E+Y++
Sbjct: 160 ELGLLFNAKQWVSPSVPNQIEAYLS 184
Score = 51.2 bits (117), Expect = 7e-07
Identities = 27/90 (30%), Positives = 49/90 (54%), Gaps = 3/90 (3%)
Frame = +3
Query: 3 DLIIFGATGFTGS---RAVLEMIRLSKKYPALTWGIAGRSEKKLEELKQEATQKSGEDQT 173
D++I GA+GFTG R L+ ++ P + +AGR+ +L + + A + + +
Sbjct: 12 DMVILGASGFTGKYVVREALKFLQTPSSSPLKSLALAGRNPTRLTQSLEWAARPN-PPPS 70
Query: 174 NLKTFIADVSDEKSLRSMCEQARVVVNVAG 263
++ AD SD SLR +C Q ++++N G
Sbjct: 71 SVAILTADTSDPDSLRRLCTQTKLILNCVG 100
Score = 29.1 bits (62), Expect = 3.2
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = +1
Query: 559 HYGTWESLVHSLANSHKLKPLRK 627
++GT+ES V +AN+ KLK LR+
Sbjct: 194 NFGTYESAVLGVANAEKLKELRR 216
>At3g18890.1 68416.m02399 expressed protein similar to UV-B and
ozone similarly regulated protein 1 UOS1 [Pisum sativum]
GI:20339364
Length = 641
Score = 34.7 bits (76), Expect = 0.064
Identities = 24/93 (25%), Positives = 46/93 (49%), Gaps = 3/93 (3%)
Frame = +3
Query: 3 DLI-IFGATGFTGSRAVLEMIRLSKKYPALTWGI--AGRSEKKLEELKQEATQKSGEDQT 173
DL+ + GATG GSR V E+++L + A AG + ++E+K + T + +
Sbjct: 82 DLVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVE 141
Query: 174 NLKTFIADVSDEKSLRSMCEQARVVVNVAGRTD 272
L+ D+ + S++ A V++ G ++
Sbjct: 142 KLEIVECDLEKKDSIQPALGNASVIICCIGASE 174
>At1g22380.1 68414.m02799 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 467
Score = 33.1 bits (72), Expect = 0.20
Identities = 16/56 (28%), Positives = 32/56 (57%)
Frame = +3
Query: 93 WGIAGRSEKKLEELKQEATQKSGEDQTNLKTFIADVSDEKSLRSMCEQARVVVNVA 260
WG+A ++ L ++ ++ +GE+ K F+A+ +D + L S C Q +V+ + A
Sbjct: 320 WGLAATGKEFLWVMRPDSV--AGEEAVIPKEFLAETADRRMLTSWCPQEKVLSHPA 373
>At4g02570.1 68417.m00351 cullin family protein similar to cullin 3
[Homo sapiens] GI:3639052; contains Pfam profile
PF00888: Cullin family
Length = 738
Score = 29.9 bits (64), Expect = 1.8
Identities = 13/38 (34%), Positives = 20/38 (52%)
Frame = +2
Query: 251 ERCGPYRLYGEQVVKAAIESKTHYVDVSGEPEFMELMQ 364
+ C Y L E+ +K E HY+ S EP+ +E +Q
Sbjct: 215 DSCPDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQ 252
>At1g22400.1 68414.m02801 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 489
Score = 29.5 bits (63), Expect = 2.4
Identities = 16/63 (25%), Positives = 30/63 (47%)
Frame = +3
Query: 72 KKYPALTWGIAGRSEKKLEELKQEATQKSGEDQTNLKTFIADVSDEKSLRSMCEQARVVV 251
K+ WG+AG ++ L ++ + +GE+ F+ + D L S C Q +V+
Sbjct: 314 KQLVEFAWGLAGSGKEFLWVIRPDLV--AGEEAMVPPDFLMETKDRSMLASWCPQEKVLS 371
Query: 252 NVA 260
+ A
Sbjct: 372 HPA 374
>At3g53540.1 68416.m05912 expressed protein
Length = 924
Score = 29.1 bits (62), Expect = 3.2
Identities = 18/60 (30%), Positives = 30/60 (50%)
Frame = -1
Query: 353 P*TQAPRSRQHSESLIRWLPSRPVLRIVGTARNVHHNSGLFAHAPQRLLIRYISYKRFEI 174
P ++P S++H +SL R +LR + R+ H N G + P R R+ SY ++
Sbjct: 237 PSLKSPNSQRHVDSLKTQKVDRDLLR--KSHRSPHRNGGGGSGCPSRSHTRHASYDTIDL 294
>At5g59240.1 68418.m07424 40S ribosomal protein S8 (RPS8B) 40S
ribosomal protein S8, Prunus armeniaca, EMBL:AF071889
Length = 210
Score = 28.7 bits (61), Expect = 4.2
Identities = 16/55 (29%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 14 IRRDRFHRQPSGAGDDQAVQEVSRFNLGHRWA-----LREETRRIKTRGYSEKWR 163
I RD H++ + G + ++ ++ LG + A + RRI+ RG + KWR
Sbjct: 3 ISRDSIHKRRATGGKQKMWRKKRKYELGRQPANTKLSSNKTVRRIRVRGGNVKWR 57
>At1g63540.1 68414.m07183 hydroxyproline-rich glycoprotein family
protein
Length = 635
Score = 28.7 bits (61), Expect = 4.2
Identities = 28/100 (28%), Positives = 43/100 (43%), Gaps = 14/100 (14%)
Frame = -3
Query: 453 ITPKSQG-----MLSNPQALMTYTPASLACSL*FSCINSINSGSPLTST**VFDSMAAFT 289
ITP S G P+ L+ + + + + S + ++S SP + + AA T
Sbjct: 101 ITPVSSGPAPSPTFGEPRILLATSGSGASATSTSSTSSPLHSSSPFSFG----SAPAAIT 156
Query: 288 TCSPYSRYGPQRSPQLWLVR---------TCSSTTSHPIH 196
+ S P SP+LW+ R T SS+TS P H
Sbjct: 157 SVSSGPAQSPASSPRLWIDRFATSSSASATSSSSTSSPFH 196
>At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 28.3 bits (60), Expect = 5.6
Identities = 17/57 (29%), Positives = 31/57 (54%), Gaps = 5/57 (8%)
Frame = +3
Query: 54 EMIRLSKKYPALTWGIAGRSEKKLEELKQEATQK-----SGEDQTNLKTFIADVSDE 209
E+ ++S + + +GR+ K+EE++ A S E +TN KT +A ++DE
Sbjct: 8 ELAKVSSSSSSSSSSSSGRASVKIEEIEGGAAASGVVIVSEELETNPKTVVASIADE 64
>At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 28.3 bits (60), Expect = 5.6
Identities = 17/57 (29%), Positives = 31/57 (54%), Gaps = 5/57 (8%)
Frame = +3
Query: 54 EMIRLSKKYPALTWGIAGRSEKKLEELKQEATQK-----SGEDQTNLKTFIADVSDE 209
E+ ++S + + +GR+ K+EE++ A S E +TN KT +A ++DE
Sbjct: 8 ELAKVSSSSSSSSSSSSGRASVKIEEIEGGAAASGVVIVSEELETNPKTVVASIADE 64
>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
protein S8 - Zea mays, PIR:T04088
Length = 222
Score = 27.9 bits (59), Expect = 7.3
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 5/55 (9%)
Frame = +2
Query: 14 IRRDRFHRQPSGAGDDQAVQEVSRFNLGHRWA-----LREETRRIKTRGYSEKWR 163
I RD H++ + G + ++ ++ +G + A + RRI+ RG + KWR
Sbjct: 3 ISRDSIHKRRATGGKQKQWRKKRKYEMGRQPANTKLSSNKTVRRIRVRGGNVKWR 57
>At3g22420.1 68416.m02829 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 568
Score = 27.9 bits (59), Expect = 7.3
Identities = 19/51 (37%), Positives = 23/51 (45%), Gaps = 2/51 (3%)
Frame = +2
Query: 296 AAIESKTHYVDVSGEPEFM--ELMQLNYNEQAKDAGVYVISACGFDSIPCD 442
AAI K+H V G PEFM E+ YNE VY C + + D
Sbjct: 175 AAILRKSHAVRCVGTPEFMAPEVYDEEYNEL---VDVYAFGMCVLEMVTFD 222
>At1g08540.1 68414.m00946 RNA polymerase sigma subunit SigB (sigB) /
sigma factor 2 (SIG2) identical to sigma factor SigB
[Arabidopsis thaliana] GI:5478446, plastid RNA
polymerase sigma-subunit [Arabidopsis thaliana]
GI:2879922, sigma factor [Arabidopsis thaliana]
GI:2597831, sigma factor 2 (SIG2) [Arabidopsis thaliana]
GI:2353173; contains Pfam profiles PF04545: Sigma-70,
region 4, PF04539: Sigma-70 region 3, PF04542: Sigma-70
region 2
Length = 572
Score = 27.9 bits (59), Expect = 7.3
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +3
Query: 120 KLEELKQEATQKSGEDQTNLKTFIADVSDEKSLR 221
KLE L+ E T++SG T + A D+KSLR
Sbjct: 290 KLERLQTELTERSGRQPTFAQWASAAGVDQKSLR 323
>At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 /
cytokinin synthase (IPT1) identical to adenylate
isopentenyltransferase (IPT1) [Arabidopsis thaliana]
GI:14279054
Length = 357
Score = 27.5 bits (58), Expect = 9.7
Identities = 17/50 (34%), Positives = 23/50 (46%), Gaps = 1/50 (2%)
Frame = +3
Query: 90 TWGIAGRSEKKLEELKQEATQKSGEDQT-NLKTFIADVSDEKSLRSMCEQ 236
TW +A R KK+E LK + D T + K + S EK R E+
Sbjct: 291 TWTLAKRQVKKIEMLKDAGWEIERVDATASFKAVMMKSSSEKKWRENWEE 340
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,879,839
Number of Sequences: 28952
Number of extensions: 317253
Number of successful extensions: 972
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 949
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 972
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1604469728
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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